Entomogenous fungi isolated from Cryptotympana atrata with antibacterial and antifungal activity.

Although many entomogenous fungi have been discovered over the years, few studies on the crude extracts of fungi isolated from Cryptotympana atrata with antibacterial and antifungal activity were reported. In this study, total twenty entomogenous fungi were isolated for the first time. And among of them, two pure cultures were identified as Purpureocillium lilacinum and Aspergillus fumigatus with apparent morphology, microscopic identification and 18S rRNA gene sequence. The active strains were fermented to optimize in six different culture media at three different pH values. The antibacterial and antifungal activities of the metabolites were more potent and efficient in Fungal medium 3# at a pH of 6.2 than in the other tested media or at the other tested pH values. Total seven human pathogens and one insect pathogen were used to evaluate the antibacterial and antifungal activity of crude extracts, among which 25% of the extracts exhibited antifungal activity against Verticillium lecanii, while 33.3% and 47.2% of the extracts exhibited antibacterial activity against the important human pathogens Staphylococcus aureus and Bacillus cereus, respectively. The range of the MICs was from 15.6 to 250 µg mL-1, and 35% of the fungal metabolites exhibited antibacterial activity against Pseudomonas aeruginosa, Bacillus thuringiensis and Enterobacter aerogenes at 1000 µg mL-1 except the previously described antibacterial activities. Furthermore, the phylogenetic relationships of the two identified fungi were also constructed. In brief, it is the first reporting about enthompathogenic fungi from Cryptotympana atrata and provides candidate strains with potential use as biological agents and against multidrug-resistant organisms.

Diversity and function of the Antarctic krill microorganisms from Euphausia superba.

The diversity and ecological function of microorganisms associated with Euphausia superba, still remain unknown. This study identified 75 microbial isolates from E. superba, that is 42 fungi and 33 bacteria including eight actinobacteria. And all the isolates showed NaF tolerance in conformity with the nature of the fluoride krill. The maximum concentration was 10%, 3% and 0.5% NaF for actinobacteria, bacteria and fungi, respectively. The results demonstrated that 82.4% bacteria, 81.3% actinobacteria and 12.3% fungi produced antibacterial metabolites against pathogenic bacteria without NaF; the MIC value reached to 3.9 µg/mL. In addition, more than 60% fungi produced cytotoxic metabolites against A549, MCF-7 or K562 cell lines. The presence of NaF led to a reduction in the producing antimicrobial compounds, but stimulated the production of cytotoxic compounds. Furthermore, seven cytotoxic compounds were identified from the metabolites of Penicillium citrinum OUCMDZ4136 under 0.5% NaF, with the IC50 values of 3.6-13.1 µM for MCF-7, 2.2-19.8 µM for A549 and 5.4-15.4 µM for K562, respectively. These results indicated that the krill microbes exert their chemical defense by producing cytotoxic compounds to the mammalians and antibacterial compounds to inhibiting the pathogenic bacteria.