6-Nitro-1,3-benzothia-zole-2(3H)-thione.

In the title mol-ecule, C(7)H(4)N(2)O(2)S(2), the nitro group is twisted by 5.5 (1)° from the plane of the attached benzene ring. In the crystal, N-H⋯S hydrogen bonds link pairs of mol-ecules into inversion dimers, which are linked by weak C-H⋯O inter-actions into sheets parallel to (101). The crystal packing exhibits short inter-molecular S⋯O contacts of 3.054 (4) Šand π-π inter-actions of 3.588 (5) Šbetween the centroids of the five- and six-membered rings of neighbouring mol-ecules.

Leaf Functional Traits of Two Species Affected by Nitrogen Addition Rate and Period Not Nitrogen Compound Type in a Meadow Grassland.

Plasticity of plant functional traits plays an important role in plant growth and survival under changing climate. However, knowledge about how leaf functional traits respond to the multi-level N addition rates, multiple N compound and duration of N application remains lacking. This study investigated the effects of 2-year and 7-year N addition on the leaf functional traits of Leymus chinensis and Thermopsis lanceolata in a meadow grassland. The results showed that the type of N compounds had no significant effect on leaf functional traits regardless of duration of N application. N addition significantly increased the leaf total N content (LN) and specific leaf area (SLA), and decreased the leaf total P content (LP) and leaf dry matter content (LDMC) of the two species. Compared with short-term N addition, long-term N addition increased LN, LP, SLA, and plant height, but decreased the LDMC. In addition, the traits of the two species were differentially responsive to N addition, LN and LP of T. lanceolata were consistently higher than those of L. chinensis. N addition would make L. chinensis and T. lanceolata tend to "quick investment-return" strategy. Our results provide more robust and comprehensive predictions of the effects of N deposition on leaf traits.

Mowing Did Not Alleviate the Negative Effect of Nitrogen Addition on the Arbuscular Mycorrhizal Fungal Community in a Temperate Meadow Grassland.

As nitrogen deposition intensifies under global climate change, understanding the responses of arbuscular mycorrhizal (AM) fungi to nitrogen deposition and the associated mechanisms are critical for terrestrial ecosystems. In this study, the effects of nitrogen addition and mowing on AM fungal communities in soil and mixed roots were investigated in an Inner Mongolia grassland. The results showed that nitrogen addition reduced the α-diversity of AM fungi in soil rather than that of root. Besides, nitrogen addition altered the composition of AM fungal community in soil. Soil pH and inorganic nitrogen content were the main causes of changes in AM fungal communities affected by nitrogen addition. Mowing and the interaction of nitrogen addition and mowing had no significant effect on AM fungal community diversity. In contrast, while mowing may reduce the negative effects of nitrogen addition on the richness and diversity of plants by alleviating light limitation, it could not do so with the negative effects on AM fungal communities. Furthermore, AM fungal communities clustered phylogenetically in all treatments in both soil and roots, indicating that environmental filtering was the main driving force for AM fungal community assembly. Our results highlight the different responses of AM fungi in the soil and roots of a grassland ecosystem to nitrogen addition and mowing. The study will improve our understanding of the effects of nitrogen deposition on the function of ecosystem.