RESUMO
The chemical composition and antibacterial activity of Thymus glabrescens Willd. (Lamiaceae) essential oil were examined, as well as the association between it and chloramphenicol. The antibacterial activities of geraniol and thymol, the main constituents of T. glabrescens oil, individually and in combination with chloramphenicol, were also determined. The interactions of the essential oil, geraniol, and thymol with chloramphenicol toward five selected strains were evaluated using the microdilution checkerboard assay in combination with chemometric methods. Oxygenated monoterpenes were the most abundant compound class in the oil, with geraniol (22.33%) as the major compound. The essential oil exhibited in vitro antibacterial activity against all tested bacterial strains, but the activities were lower than those of the standard antibiotic and thymol. A combination of T. glabrescens oil and chloramphenicol produced a strong synergistic interaction (FIC indices in the range 0.21-0.87) and a substantial reduction of the MIC value of chloramphenicol, thus minimizing its adverse side effects. The combinations geraniol-chloramphenicol and thymol-chloramphenicol produced synergistic interaction to a greater extent, compared with essential oil-chloramphenicol association, which may indicate that the activity of the thyme oil could be attributed to the presence of significant concentrations of geraniol and thymol.
Assuntos
Antibacterianos/farmacologia , Cloranfenicol/farmacologia , Óleos Voláteis/farmacologia , Timo/química , Cromatografia Gasosa , Sinergismo Farmacológico , Técnicas In Vitro , Testes de Sensibilidade MicrobianaRESUMO
As a tobacco producer, Serbia has to deal with large amounts of leftover tobacco stalks after harvesting. One option for this type of biomass is to burn it, but burning is not encouraged in Serbia, since the levels of its combustion products have not been investigated yet. The aim of this study was therefore to determine the elemental composition, ash and nicotine content, heat values, and composition of gaseous combustion products of tobacco stalk briquettes and to see if their mixing with other types of biomass available in Serbia could improve their ecological profile. We made 11 different types of briquettes: six of pure raw materials, including burley tobacco stalks, sunflower head remains, wheat straw, corncob, soy straw, and beech sawdust and five by mixing tobacco stalks with these other raw materials in a 50:50 mass ratio. All briquettes meet the ecological criteria regarding the emission limits for nitrogen oxides (NOx), sulphur dioxide, carbon monoxide, and carbon dioxide. Nicotine content in flue gases (<10 mg/kg) is far below the maximum level allowed by the European Union. Heat values of all biomass samples are acceptable, although lower than those specified for solid biofuels (≥16.0 MJ/kg), save for corncob and beech sawdust and their mixtures with tobacco stalks. Our findings therefore encourage the use of tobacco stalks as a viable biofuel.
Assuntos
Carvão Mineral , Nicotiana , Biomassa , Nicotina , Dióxido de EnxofreRESUMO
The antibacterial effects of Thymus vulgaris (Lamiaceae), Lavandula angustifolia (Lamiaceae), and Calamintha nepeta (Lamiaceae) Savi subsp. nepeta var. subisodonda (Borb.) Hayek essential oils on five different bacteria were estimated. Laboratory control strain and clinical isolates from different pathogenic media were researched by broth microdilution method, with an emphasis on a chemical composition-antibacterial activity relationship. The main constituents of thyme oil were thymol (59.95%) and p-cymene (18.34%). Linalool acetate (38.23%) and ß-linalool (35.01%) were main compounds in lavender oil. C. nepeta essential oil was characterized by a high percentage of piperitone oxide (59.07%) and limonene (9.05%). Essential oils have been found to have antimicrobial activity against all tested microorganisms. Classification and comparison of essential oils on the basis of their chemical composition and antibacterial activity were made by utilization of appropriate chemometric methods. The chemical principal component analysis (PCA) and hierachical cluster analysis (HCA) separated essential oils into two groups and two sub-groups. Thyme essential oil forms separate chemical HCA group and exhibits highest antibacterial activity, similar to tetracycline. Essential oils of lavender and C. nepeta in the same chemical HCA group were classified in different groups, within antibacterial PCA and HCA analyses. Lavender oil exhibits higher antibacterial ability in comparison with C. nepeta essential oil, probably based on the concept of synergistic activity of essential oil components.
Assuntos
Antibacterianos/química , Cromatografia Gasosa-Espectrometria de Massas/métodos , Lavandula/química , Óleos Voláteis/química , Óleos de Plantas/química , Timol/química , Thymus (Planta)/química , Antibacterianos/farmacologia , Bactérias/efeitos dos fármacos , Óleos Voláteis/farmacologia , Óleos de Plantas/farmacologiaRESUMO
Photocatalytic degradation of synthesized azo pyridone dye (5-(4-sulpho phenylazo)-6-hydroxy-4-methyl-3-cyano-2-pyridone), in aqueous solutions by simulated sunlight in the presence of commercial TiO(2), Aeroxide P25, was studied. The reaction kinetics analysis showed that photodegradation exhibits pseudo first-order kinetics according to Langmuir-Hinshelwood model. The effects of various process parameters on the photocatalytic degradation were investigated. The optimal catalyst content and pH were determined. A decrease in the reaction rate was observed upon the increase of the initial dye concentration. Degradation of the dye was enhanced by hydrogen peroxide, but it was inhibited by ethanol. The influence of temperature was studied, and the energy of activation was determined. According to total organic carbon (TOC) analysis, 54% of TOC remained when 100% of the dye was decolorized. Although the intermediates were not determined in this study, the TOC results clearly indicate their presence during the reaction. In addition, photocatalytic degradation of simulated dyehouse effluents, containing tested azo pyridone dye and associated auxiliary chemicals was investigated.
Assuntos
Compostos Azo/química , Corantes/química , Fotólise , Luz Solar , Titânio/químicaRESUMO
The main biologically active components of plants belonging to the genus Allium, responsible for their biological activities, including anti-inflammatory, antioxidant and immunomodulatory, are organosulfur compounds. The aim of this study was to synthetize the mixture of dipropyl polysulfides (DPPS) and to test their biological activity in acute hepatitis. C57BL/6 mice were administered orally with DPPS 6 h before intravenous injection of Concanavalin A (ConA). Liver inflammation, necrosis and hepatocytes apoptosis were determined by histological analyses. Cytokines in liver tissue were determined by ELISA, expression of adhesive molecules and enzymes by RT PCR, while liver mononuclear cells were analyzed by flow cytometry. DPPS pretreatment significantly attenuated liver inflammation and injury, as evidenced by biochemical and histopathological observations. In DPPS-pretreated mice, messenger RNA levels of adhesion molecules and NADPH oxidase complex were significantly reduced, while the expression of SOD enzymes was enhanced. DPPS pretreatment decreased protein level of inflammatory cytokines and increased percentage of T regulatory cells in the livers of ConA mice. DPPS showed hepatoprotective effects in ConA-induced hepatitis, characterized by attenuation of inflammation and affection of Th17/Treg balance in favor of T regulatory cells and implicating potential therapeutic usage of DPPS mixture in inflammatory liver diseases.
Assuntos
Anti-Inflamatórios/uso terapêutico , Hepatite/tratamento farmacológico , Fígado/efeitos dos fármacos , Propano/análogos & derivados , Sulfetos/uso terapêutico , Animais , Apoptose , Concanavalina A , Modelos Animais de Doenças , Hepatite/complicações , Hepatite/patologia , Hepatócitos/efeitos dos fármacos , Hepatócitos/patologia , Inflamação/etiologia , Inflamação/prevenção & controle , Fígado/patologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Necrose , Propano/uso terapêuticoRESUMO
It was demonstrated that during the growth of Aureobasidium pullulans strain CH-1 on the acid hydrolysate of peat from the Vlasina Lake, the content of metals (Cu, Fe, Zn, Mn, Pb, Cd, Ni and Cr) decreased due to biosorption. The reduction in the metal content was found to be in the range (%): 38.2-62.2, 67.7-97.3, 0.02-62.05, 0.05-23.97, 0.16-4.24, 3.45-51.72, 1.18-35.82, 0.86-44.44, for Cu, Fe, Zn, Mn, Pb, Cd, Ni and Cr, respectively. During this process, the metals were accumulated in the biomass, while pullulan, an extracellular polysaccharide produced by Aureobasidium pullulans strain CH-1, was found not to bind the above-mentioned metals.
Assuntos
Ascomicetos/metabolismo , Glucanos/biossíntese , Metais/metabolismo , Solo , Adsorção , Cromatografia Gasosa , Hidrólise , Espectrofotometria Atômica , Espectroscopia de Infravermelho com Transformada de FourierRESUMO
The composition and antimicrobial activity of the essential oil of Heracleum sibiricum L. (Apiaceae) was studied. The aerial part of plant was hydro-distilled and chemical composition of the essential oil was analyzed by GC and GC-MS. Forty-six compounds, corresponding to 95.12% of the total oil, were identified. Esters represented the major chemical class (69.55%) while the main constituents were octyl butanoate (36.82%), hexyl butanoate (16.08%), 1-octanol (13.62%) and octyl hexanoate (8.10%). Antibacterial activity of the essential oil and reference antibiotics against nine bacterial strains was tested by the broth microdilution method. The results of the bioassays showed that essential oil had slight antimicrobial activities against all tested microorganisms (MIC and MBC values were in the range of 2431.2 to 9724.8 microg/mL). Reference antibiotics were active in concentrations between 0.5 and 16.0 microg/mL. The results confirm that Gram-positive bacteria were more susceptible to the essential oil of H. sibiricum, in comparison with Gram-negative bacteria.