Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 21
Filtrar
1.
Zhongguo Zhong Yao Za Zhi ; 48(7): 1815-1823, 2023 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-37282956

RESUMO

It is generally believed that high-quality Bupleurum scorzonerifolium roots possess specific morphological characteristics, being red, robust, and long with strong odor. However, the scientific connotation of these characteristics has not been elucidated. According to the theory of "quality evaluation through morphological identification", we studied the correlations between appearance traits(the RGB value of root surface, root length, root diameter, dry weight, and ratio of phloem to xylem) and content of main chemical components(volatile oils, total saponins, total flavonoids, total polysaccharides, and seven saikosaponins) of B. scorzonerifolium roots. Epson Scanner and ImageJ were used to scan the root samples and measure the appearance traits. Ultraviolet spectrophotometry and HPLC were employed to determine the content of chemical components. The correlation, regression, and cluster analyses were performed to study the correlations between the appearance traits and the content of chemical components. The results showed that the content of volatile oils and saikosaponins were significantly correlated with RGB value, root length, and root diameter, indicating that within a certain range, the roots being redder, longer, and thicker had higher content of volatile oils and saikosaponins. According to the appearance traits and chemical component content, the 14 samples from different producing areas were classified into four grades, and the differences in morphological traits and chemical component content were consistent among different grades. The findings in this study demonstrate that appearance traits(RGB value, root length, and root diameter) can be used to evaluate the quality of B. scorzonerifolium roots. Meanwhile, this study lays a foundation for establishing an objective quality evaluation method for B. scorzonerifolium roots.


Assuntos
Bupleurum , Óleos Voláteis , Ácido Oleanólico , Saponinas , Bupleurum/química , Saponinas/análise , Ácido Oleanólico/análise , Óleos Voláteis/análise , Raízes de Plantas/química
2.
World J Surg Oncol ; 19(1): 273, 2021 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-34507558

RESUMO

BACKGROUND: Breast cancer is the most common malignancy in women. Cancer driver gene-mediated alterations in the tumor microenvironment are critical factors affecting the biological behavior of breast cancer. The purpose of this study was to identify the expression characteristics and prognostic value of cancer driver genes in breast cancer. METHODS: The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) datasets are used as the training and test sets. Classified according to cancer and paracancerous tissues, we identified differentially expressed cancer driver genes. We further screened prognosis-associated genes, and candidate genes were submitted for the construction of a risk signature. Functional enrichment analysis and transcriptional regulatory networks were performed to search for possible mechanisms by which cancer driver genes affect breast cancer prognosis. RESULTS: We identified more than 200 differentially expressed driver genes and 27 prognosis-related genes. High-risk group patients had a lower survival rate compared to the low-risk group (P<0.05), and risk signature showed high specificity and sensitivity in predicting the patient prognosis (AUC 0.790). Multivariate regression analysis suggested that risk scores can independently predict patient prognosis. Further, we found differences in PD-1 expression, immune score, and stromal score among different risk groups. CONCLUSION: Our study confirms the critical prognosis role of cancer driver genes in breast cancer. The cancer driver gene risk signature may provide a novel biomarker for clinical treatment strategy and survival prediction of breast cancer.


Assuntos
Neoplasias da Mama , Microambiente Tumoral , Neoplasias da Mama/genética , Feminino , Perfilação da Expressão Gênica , Humanos , Prognóstico , Taxa de Sobrevida , Microambiente Tumoral/genética
3.
J Cell Biochem ; 119(11): 9284-9294, 2018 11.
Artigo em Inglês | MEDLINE | ID: mdl-30010211

RESUMO

This study aims to explore the effects of Notch1 gene on remyelination in multiple sclerosis (MS). A mouse model of acute demyelination was successfully established and the model mice were grouped as cuprizone (CPZ) group, CPZ + small interfering RNA (siRNA)-Notch1 (siNotch1) group, and CPZ + siRNA negative control (NC) group. Meanwhile, another 3 groups (control, control + siNotch1, and control + siRNA NC) were established in normal mice. The changes of weight and maintenance time in rotating drum of mice were observed. Western blot analysis for the protein expressions related to Notch signaling pathway and oligodendrocyte (OL) differentiation in the corpus callosum of the mice. After model establishment, the weight of CPZ-induced demyelinated mice was decreased. During the repair period, the balance ability and movement of the mice was recovered, especially for those injected with siNotch1 plasmid. After model establishment, the number of myelinated axons was decreased. In comparison with the CPZ and CPZ siRNA NC groups, the CPZ + siNotch1 group had a decrease in the number of premature OLs, but increase in mature OLs, and a decrease in oligodendrocyte precursor cells and astrocytes. The expressions of proteins related to Notch signaling pathway, such as HES, Jagged-1 were decreased in the CPZ + siNotch1 group in contrast to the CPZ and CPZ + siRNA groups, but the OL-related transcription factor Sox10 was increased in the CPZ + siNotch1 group than in the CPZ + siRNA NC and CPZ groups, and Id2 was decreased. Our study provided evidence that the inhibition of Notch1 gene could accelerate remyelination in MS.


Assuntos
Doenças Desmielinizantes/metabolismo , Esclerose Múltipla/metabolismo , Receptor Notch1/metabolismo , Animais , Western Blotting , Doenças Desmielinizantes/genética , Imuno-Histoquímica , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Esclerose Múltipla/genética , RNA Interferente Pequeno/genética , RNA Interferente Pequeno/metabolismo , Distribuição Aleatória , Receptor Notch1/genética
4.
Opt Express ; 25(15): 18009-18016, 2017 Jul 24.
Artigo em Inglês | MEDLINE | ID: mdl-28789288

RESUMO

Electro-optical properties of polymer-stabilized sphere phase liquid crystal (PS-SPLC) switching devices are analyzed and validated experimentally. The experimental results show the voltage-dependent transmittance curves of PS-SPLC devices. A diffraction approach, called extended anomalous diffraction approach, is proposed to fit the experimental data. Good agreement between experiment and model is obtained. The scattering model provides practical guidance for the improvement of PS-SPLC displays performance and optimization.

5.
Zhongguo Zhong Yao Za Zhi ; 40(20): 4108-11, 2015 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-27062834

RESUMO

The eight heavy metals and two essential constitutes of safflowers planted in linzhi which lies in Southern Tibet were analyzed by ICP-MS and by HPLC respectively. Heavy metals of safflower in the region were at the lower level and the essential constitutes were at the higher level. The better quality of safflower here was assisted by the excellent climate in tibet.


Assuntos
Carthamus tinctorius/química , Medicamentos de Ervas Chinesas/análise , Flores/química , Metais Pesados/análise , Cromatografia Líquida de Alta Pressão , Contaminação de Medicamentos , Tibet
6.
Curr Microbiol ; 69(4): 517-24, 2014 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-24894904

RESUMO

Strain 39 is an endophytic fungus which was isolated from Dioscorea nipponica Makino (DNM). After Strain 39 co-cultured with ethanol extract of DNM rhizomes for several days, the content of saponins in this culture mixture would be obviously increased. To analyze the mechanism of this microbial transformation, we used the differential display reverse transcription polymerase chain reaction (DDRT-PCR) method to compare the transcriptomes between Strain 39 cultured in normal PD medium and in PD medium which added ethanol extract of DNM rhizomes. We amplified 29 DDRT-PCR bands using 12 primer combinations of three anchored primers and five random primers, and six bands were re-amplified. Analysis of real-time PCR and sequence alignment showed that three clones were up-regulated in sample group: squalene epoxidase, squalene synthase, and catalase, one clone was expressed only in sample group. The possible roles and origins of the above genes were discussed, and the molecular mechanism of Strain 39 biotransformation was speculated. This study is the first report of the molecular biotransformation mechanism of saponins production by endophytic fungus of DNM.


Assuntos
Dioscorea/microbiologia , Endófitos/genética , Proteínas Fúngicas/genética , Fungos/genética , Dioscorea/química , Endófitos/classificação , Endófitos/isolamento & purificação , Endófitos/metabolismo , Proteínas Fúngicas/metabolismo , Fungos/classificação , Fungos/isolamento & purificação , Fungos/metabolismo , Regulação Fúngica da Expressão Gênica , Extratos Vegetais/metabolismo , Saponinas/metabolismo
7.
Biol Pharm Bull ; 36(11): 1700-7, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24189414

RESUMO

Metabolomics is a new platform based on the comprehensive analysis of low molecular weight metabolites and provides a powerful approach to discover biomarkers in biological systems. Modified Sinisan (MSNS), a traditional Chinese medicine formula, displayed bright prospects in the prevention and therapy of liver injury. However, its molecular mechanism of hepatoprotective effects remains unclear. This paper was designed to explore the effects and potential mechanisms of MSNS against dimethylnitrosamine-induced liver injury. Global metabolic profiling was performed by ultra-performance liquid chromatography/electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC/ESI-Q-TOF-MS) in conjunction with multivariate data analysis and pathway analysis. Eleven serum biomarkers were identified and pathway analysis results showed that phenylalanine, tyrosine and tryptophan biosynthesis, phenylalanine metabolism, tryptophan metabolism, retinol metabolism, tyrosine metabolism were perturbed by liver injury. More importantly, MSNS has showed satisfactory pharmacological effect on liver injury through partially regulating the perturbed pathways, correlates well to the biochemical and histopathological detection results. The present study proved that the robust metabolomics approach is promising for unraveling hepatoprotective effects of MSNS and these findings provide new insights into mechanisms of the liver injury, and its pathophysiologic processes.


Assuntos
Doença Hepática Induzida por Substâncias e Drogas/sangue , Medicamentos de Ervas Chinesas/farmacologia , Substâncias Protetoras/farmacologia , Animais , Biomarcadores/sangue , Doença Hepática Induzida por Substâncias e Drogas/tratamento farmacológico , Doença Hepática Induzida por Substâncias e Drogas/patologia , Dimetilnitrosamina , Medicamentos de Ervas Chinesas/uso terapêutico , Fígado/efeitos dos fármacos , Fígado/patologia , Masculino , Metabolômica , Fitoterapia , Substâncias Protetoras/uso terapêutico , Ratos , Ratos Wistar
8.
BMC Complement Altern Med ; 13: 252, 2013 Oct 03.
Artigo em Inglês | MEDLINE | ID: mdl-24090456

RESUMO

BACKGROUND: Zhuyeqing Liquor (ZYQL), a well-known Chinese traditional health liquor, has various biological properties, including anti-oxidant, anti-inflammatory, immunoenhancement and cardiovascular protective effects. METHODS: The protective effects of Zhuyeqing Liquor (ZYQL) on the immune function was investigated in vivo in normal healthy mice and immunosuppressed mice treated with Cyclophosphamide (Cy, 100 mg/kg) by intraperitoneal injection on days 4, 8 and 12. ZYQL (100, 200 and 400 mg/kg) was administered via gavage daily for 14 days. The phagocytotic function of mononuclear phagocytic system was detected with carbon clearance methods, the levels of interleukin-6 (IL-6) and interferon-gamma (IFN-γ) in serum were detected with Enzyme linked immunosorbent assay (ELISA). Immune organs were weighed and organ indexes (organ weight/body weight) of thymus and spleen were calculated. Meanwhile, the activity of lysozyme (LSZ) in serum and the activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) in spleen tissue were measured. RESULTS: ZYQL significantly upgrades the K value for clearance of carbon particles in normal mice treated with ZYQL (400 mg/kg) and immunosuppressed mice treated with ZYQL (100, 200 and 400 mg/kg) together with Cy (100 mg/kg) in vivo. The treatment of ZYQL (100, 200 and 400 mg/kg) effectively increased the activity of serum lysozyme as well as promoted the serum levels of IL-6 and IFN-γ in normal mice and immunosuppressed mice. Furthermore, ZYQL (100, 200 and 400 mg/kg) had an antioxidant effects in immune system by enhancing the antioxidant enzyme activity of SOD, CAT and GSH-Px in vivo. In addition, ZYQL (100, 200 and 400 mg/kg) effectively elevated the Cy-induced decreased organ index (thymus and spleen). CONCLUSIONS: The present work shows that the dose-dependent administration of ZYQL is capable of influencing immune responses, which implying that its valuable functional health may be attributed partly to its protective effects for the immune function.


Assuntos
Medicamentos de Ervas Chinesas/farmacologia , Substâncias Protetoras/farmacologia , Análise de Variância , Animais , Carbono/farmacocinética , Citocinas/sangue , Hospedeiro Imunocomprometido , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Muramidase/sangue , Fagocitose/efeitos dos fármacos , Baço/efeitos dos fármacos , Baço/enzimologia , Distribuição Tecidual
9.
Curr Top Med Chem ; 23(16): 1522-1541, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37638613

RESUMO

BACKGROUND: Artemisia argyi Lévl. et Van., Artemisia princeps Pamp., and Artemisia montana Pamp., which are the sources of mugwort, have been popular across East Asian countries for nearly 2000 years now. Essential oils are the major chemical component obtained from them, exhibiting a variety of biological activities. OBJECTIVE: This review mainly focuses on the chemical composition and biological activities of A. argyi essential oil (AAEO), A. princeps essential oil (APEO), and A. montana essential oil (AMEO), with a special focus on their common and specific characteristics. The traditional use, distribution, and botany of A. argyi, A. princeps, and A. montana have also been summarized. In addition, the pharmacokinetics of AAEO was involved. METHODS: We collected literature from online and offline databases by entering the following keywords: mugwort, wormwood, A. argyi, A. princeps, A. montana, essential oil, and volatile oil. No language limitation was present in our search. RESULTS: A. argyi, A. princeps, and A. montana were used as traditional medicine, food, and health care products for a long time in Asia. They are widely distributed in most parts of China, Korea, and Japan. AAEO, APEO, and AMEO composed of monoterpenes, sesquiterpenes and their derivatives, alkanes, olefins, etc. Most of the specific compounds of AAEO were monoterpenoids, nearly half of the specific compounds of APEO were aliphatic hydrocarbons, and the sesquiterpenes were the typical specific compounds of AMEO. The mugwort essential oil showed multiple biological activities, such as anti-microbial, anti-inflammatory, antioxidant, antitumor, anticoagulation, sedative, and insecticide. CONCLUSION: The present review provided insight into the chemical composition and biological activity of AAEO, APEO, and AMEO. The comprehensive literature showed that they possessed wide application prospects in various fields. However, they should be studied in more depth. The underlying bioactive mechanisms should be elucidated and their toxicity and quality control should be determined.


Assuntos
Artemisia , Óleos Voláteis , Óleos Voláteis/farmacologia , Montana , Óleos de Plantas/farmacologia , Alcanos
10.
Sci Rep ; 11(1): 18201, 2021 09 14.
Artigo em Inglês | MEDLINE | ID: mdl-34521875

RESUMO

Panax ginseng is one of the oldest and most generally prescribed herbs in Eastern traditional medicine to treat diseases. Several studies had documented that ginseng leaves have anti-oxidative, anti-inflammatory, and anticancer properties similar to those of ginseng root. The aim of this research was to forecast of the molecular mechanism of ginseng leaves on lung cancer by molecular docking and network pharmacology so as to decipher ginseng leaves' entire mechanism. The compounds associated with ginseng leaves were searched by TCMSP. TCMSP and Swiss Target Prediction databases were used to sort out the potential targets of the main chemical components. Targets were collected from OMIM, PharmGKB, TTD, DrugBank and GeneCards which related to immunity and lung cancer. Ginseng leaves exert its lung cancer suppressive function by regulating the several signaling proteins, such as JUN, STAT3, AKT1, TNF, MAPK1, TP53. GO and KEGG analyses indicated that the immunoreaction against lung cancer by ginseng leaves might be related to response to lipopolysaccharide, response to oxidative stress, PI3K-Akt, MAPK and TNF pathway. Molecular docking analysis demonstrated that hydrogen bonding was interaction's core forms. The results of CCK8 test and qRT-PCR showed that ginseng leaves inhibit cell proliferation and regulates AKT1 and P53 expression in A549. The present study clarifies the mechanism of Ginseng leaves against lung cancer and provides evidence to support its clinical use.


Assuntos
Antineoplásicos/farmacologia , Fatores Imunológicos/farmacologia , Neoplasias Pulmonares/metabolismo , Panax/química , Extratos Vegetais/farmacologia , Células A549 , Proliferação de Células/efeitos dos fármacos , Humanos , Proteína Quinase 1 Ativada por Mitógeno/genética , Proteína Quinase 1 Ativada por Mitógeno/metabolismo , Folhas de Planta/química , Proteínas Proto-Oncogênicas c-akt/genética , Proteínas Proto-Oncogênicas c-akt/metabolismo , Transdução de Sinais/efeitos dos fármacos , Transcriptoma/efeitos dos fármacos , Fator de Necrose Tumoral alfa/genética , Fator de Necrose Tumoral alfa/metabolismo , Proteína Supressora de Tumor p53/genética , Proteína Supressora de Tumor p53/metabolismo
11.
Ann Transl Med ; 8(12): 751, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32647676

RESUMO

BACKGROUND: The abnormal expression of genes is an essential factor affecting the prognosis of cancer. RNA modification is a way of regulating post-transcriptional levels, including m6A, m5C, m1A RNA methylation. Studies have found that RNA methylation regulates tumorigenesis development and stem cell regeneration. However, there are few studies on lung adenocarcinoma. This study aims to explore the clinical value of RNA methylation for lung adenocarcinoma. METHODS: We summarized thirty-one RNA methylation regulators. The training set was obtained from The Cancer Genome Atlas (TCGA) database, and the test set was obtained from the Gene Expression Omnibus (GEO) database. The Wilcoxon test was used to analyze the expression of RNA methylation regulators. We constructed tumor subgroup models and risk models based on the expression of those regulators. Principal component analysis (PCA) and the receiver operating characteristic (ROC) confirmed the accuracy of the models. Real-time polymerase chain reaction (PCR) validates the results in vitro. RESULTS: Most RNA methylation regulators had distinct expressions in tumor tissues and adjacent tissues (P<0.05). All the models showed high predictive performance (AUC: 0.65-0.82), and the five-year survival of patients in each group was statistically different (P<0.05). The patients in the high-risk group were more likely to have a higher stage, more lymph node metastases, and distant metastases, showing a poor clinical outcome. Patients with high expression of NOP2 or HNRNP were more likely to have a poorly differentiated in vitro experiment. CONCLUSIONS: With our study, we found that the expressions of most RNA methylation regulators were significantly different in cancer and para-cancerous tissues. Different molecular phenotypes constructed by RNA methylation regulators can be independent risk factors for the prognosis of lung adenocarcinoma. Our study demonstrates the critical role of RNA methylation in lung adenocarcinoma, and it is expected to supply a reference for the prognostic stratification and treatment strategy development of lung adenocarcinoma.

12.
ACS Appl Mater Interfaces ; 11(24): 22015-22020, 2019 Jun 19.
Artigo em Inglês | MEDLINE | ID: mdl-31132240

RESUMO

Symmetric continuously tunable three-dimensional (3D) liquid photonic crystals have been investigated using self-organized blue-phase liquid crystal films. The photonic band gap in the overall visible spectrum can be tuned continuously, reversibly, and rapidly as the applied electric field changes. After driven by the applied field, four-time enhancement of the reflectivity results in more vivid reflection colors. A lasing emission of tuning working wavelength has been demonstrated by using the dye-doped blue-phase liquid crystal film. With the advantages of fast response speed, no alignment layer, large-scale electrically shift of the photonic band gap, and macro optical isotropy, this self-assembled soft material has many potential applications in high-performance reflective full-color display, 3D tunable lasers, and nonlinear optics.

13.
Chin J Nat Med ; 17(2): 131-144, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30797419

RESUMO

Anemone flaccida Fr. Schmidt is a perennial medicinal herb that contains pentacyclic triterpenoid saponins as the major bioactive constituents. In China, the rhizomes are used as treatments for a variety of ailments including arthritis. However, yields of the saponins are low, and little is known about the plant's genetic background or phytohormonal responsiveness. Using one-quarter of the 454 pyrosequencing information from the Roche GS FLX Titanium platform, we performed a transcriptomic analysis to identify 157 genes putatively encoding 26 enzymes involved in the synthesis of the bioactive compounds. It was revealed that there are two biosynthetic pathways of triterpene saponins in A. flaccida. One pathway depends on ß-amyrin synthase and is similar to that found in other plants. The second, subsidiary ("backburner") pathway is catalyzed by camelliol C synthase and yields ß-amyrin as minor byproduct. Both pathways used cytochrome P450-dependent monooxygenases (CYPs) and family 1 uridine diphosphate glycosyltransferases (UGTs) to modify the triterpenoid backbone. The expression of CYPs and UGTs were quite different in roots treated with the phytohormones methyl jasmonate, salicylic acid and indole-3-acetic acid. This study provides the first large-scale transcriptional dataset for the biosynthetic pathways of triterpene saponins and their phytohormonal responsiveness in the genus Anemone.


Assuntos
Anemone/genética , Vias Biossintéticas/efeitos dos fármacos , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Reguladores de Crescimento de Plantas/farmacologia , Saponinas/metabolismo , Triterpenos/metabolismo , Anemone/efeitos dos fármacos , Anemone/metabolismo , Vias Biossintéticas/genética , Sistema Enzimático do Citocromo P-450/genética , Sistema Enzimático do Citocromo P-450/metabolismo , Perfilação da Expressão Gênica , Glicosiltransferases/genética , Glicosiltransferases/metabolismo , Ácido Oleanólico/análogos & derivados , Ácido Oleanólico/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Plantas Medicinais , Rizoma/efeitos dos fármacos , Rizoma/genética , Rizoma/metabolismo
14.
Chin J Nat Med ; 16(7): 525-545, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30080652

RESUMO

The present study was designed to identify and characterize the major constituents in Juglans mandshurica Maxim. A simple, efficient and sensitive ultra performance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC-ESI-Q-TOF/MS) method was established and validated under positive and negative ion modes. The separation was performed on a Waters ACQUITY UPLC BEH C18 column (2.1 mm × 100 mm, 1.7 µm) by gradient elution with a mobile phase (Phase A: 0.1% aqueous formic acid solution, Phase B: 0.1% formic acid acetonitrile solution). A total of 165 compounds were rapidly selected by Targeted and Non-Targeted Peak Finding approaches, and then tentatively identifled by comparing with reference substances or inferred through mass spectrometry fragment ion analysis and literature data. These compounds included 68 naphthalenequinones, 20 diarylheptanoids, 29 flavonoids, 20 triterpenes, and 28 phenolic acids. In conclusion, the present study provided an effective approach to identifying components in complex matrices of herbal medicines such as Juglans mandshurica Maxim.


Assuntos
Cromatografia Líquida de Alta Pressão , Frutas/química , Juglans/química , Espectrometria de Massas em Tandem , Bases de Dados Factuais , Diarileptanoides/química , Medicamentos de Ervas Chinesas/química , Flavonoides/química , Hidroxibenzoatos/química , Estrutura Molecular , Naftoquinonas/química , Reprodutibilidade dos Testes , Triterpenos/química
15.
Zhong Yao Cai ; 30(8): 977-80, 2007 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-18074850

RESUMO

OBJECTIVE: To study the pharmacological effects of volatile oil of Valeriana amurensis on central nervous system. METHODS: The pharmacological effects of volatile oil of Valeriana amurensis on the autonomic activities of mice, the sleeping synergistic action of mice with pentoharbital sodium at subthreshold and hypnotic dosages, the sleep phases of rats, the writhing response of mice caused by acetic acid and the convulsion of mice induced by thio-semicarbazide were investigated. RESULTS: The autonomic activities of mice were significantly inhibited by the volatile oil of Valeriana amurensis. The rate of falling sleep and the extension of sleeping time of mice were significantly increased by the synergic action of pentobarbital sodium with the volatile oil. The sleep phases of SWS2 and REMS of rats were obviously extended by the volatile oil of Valeriana amurensis. In addition, the frequency of writhing response of mice caused by acetic acid was reduced, and the convulsion of mice induced by thio-semicarbazide was antagonized with the administration of the volatile oil. CONCLUSION: The volatile oil of Valeriana amurensis has significantly sedative analgesic and anti-hyperspasmia effects.


Assuntos
Fármacos do Sistema Nervoso Central/farmacologia , Sistema Nervoso Central/efeitos dos fármacos , Óleos Voláteis/farmacologia , Valeriana/química , Animais , Fármacos do Sistema Nervoso Central/administração & dosagem , Sinergismo Farmacológico , Medicamentos de Ervas Chinesas/administração & dosagem , Medicamentos de Ervas Chinesas/farmacologia , Feminino , Masculino , Camundongos , Atividade Motora/efeitos dos fármacos , Óleos Voláteis/administração & dosagem , Óleos Voláteis/isolamento & purificação , Pentobarbital/administração & dosagem , Pentobarbital/farmacologia , Raízes de Plantas/química , Ratos , Ratos Wistar , Convulsões/induzido quimicamente , Convulsões/prevenção & controle , Semicarbazidas , Sono/efeitos dos fármacos , Sono/fisiologia
16.
Zhongguo Zhong Yao Za Zhi ; 31(17): 1431-3, 2006 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-17087083

RESUMO

OBJECTIVE: To establish the methods of identification and assay in Qianshan Huoxue Gao. METHOD: Using TLC to identify Sanchi, Dragon's Blood and using HPLC to determine the content of ginsenoside Rg1. RESULT: The linear range of ginsenoside Rg1 was from 0.153 9 to 1.026 microg. The average recovery was 97.4%, RSD was 2.1%. CONCLUSION: The methods are simple and have good reproducibility.


Assuntos
Arecaceae/química , Medicamentos de Ervas Chinesas/administração & dosagem , Ginsenosídeos/análise , Panax notoginseng/química , Plantas Medicinais/química , Administração Tópica , Cromatografia Líquida de Alta Pressão , Cromatografia em Camada Fina , Combinação de Medicamentos , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/isolamento & purificação , Controle de Qualidade , Reprodutibilidade dos Testes
17.
Arch Pharm Res ; 38(5): 683-90, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-24862651

RESUMO

An unprecedented new skeleton compound (1R, 10R, 11S)-10,11-dimethyl-4-formyl-2,9-dioxa-bicyclo [5.4.0] undeca-4,6-dien-3-one (1), monoterpenoids and monoterpene glycoside picrocrocinic ester (2), epijasminoside B (3) and 6'-O-(3-methoxyl-4-hydroxyl-coumaroyl)-epijasminoside B (4), along with 26 known compounds, were obtained from Zhuyeqing Liquor. These compounds were identified mainly by analyzing their NMR, HR-ESI-MS and CD data. The isolated compounds were screened against alcohol induced HepaG 2 toxicity for hepatoprotective assay. Compounds 10, 19, 21 and 26 displayed the highest potency against alcohol induced HepaG 2 toxicity with the cell viability ratio 41.21, 56.91, 67.69 and 70.32% respectively.


Assuntos
Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/isolamento & purificação , Etanol/antagonistas & inibidores , Etanol/toxicidade , Hepatócitos/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Medicamentos de Ervas Chinesas/farmacologia , Células Hep G2 , Hepatócitos/patologia , Humanos
18.
J Microbiol ; 51(4): 430-8, 2013 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-23990293

RESUMO

Fermented foods and beverages are important parts of human diet. Fen liquor, a Chinese liquor is a fermented beverage that uses a traditional fermentation process. Starters are the main microbial source and also provide nutrients for microorganisms during fermentation. In this study, starters of Fen liquor were produced through a complex traditional fermentation process. To investigate the community structure and the composition of microorganisms in the starter production process, bacterial 16S rRNA and fungal internal transcribed spacer (ITS) regions were sequenced using clone libraries and pyrosequencing, respectively. There was much higher diversity among the bacteria than among the fungi in the starter production process. Bacteria on the surface of the starters belonged mostly to the Lactobacillaceae family, while members of the Bacillacae family were dominant in the interior of the samples that lacked access to air and water. In the fungi population, diversity was high only in the raw material. In all other samples, nearly all of the fungal sequences were from Pichia kudriavzevii, a member of the Saccharomycetaceae family. Nearly all samples showed similar fungal community structures, indicating that there was little change in the fungal community. To the best of our knowledge, this is the first report to reveal the whole process of the starter production of Chinese traditional liquor. The findings obtained in this study provide new insights into understanding the composition of the microbial community during the traditional Chinese liquor starter production process and information about the production process control and monitoring.


Assuntos
Bebidas Alcoólicas/microbiologia , Bactérias/classificação , Biodiversidade , Fungos/classificação , Bactérias/genética , China , DNA Fúngico , DNA Espaçador Ribossômico , Fungos/genética , Humanos , Microbiota , Dados de Sequência Molecular , Filogenia , RNA Bacteriano , RNA Ribossômico 16S/genética
19.
J Inflamm (Lond) ; 10(1): 30, 2013 Oct 03.
Artigo em Inglês | MEDLINE | ID: mdl-24090365

RESUMO

The study first evaluated the hepatoprotective effect of Zhuyeqing Liquor (ZYQL) against acute alcohol-induced liver injury in mice. Animals were administered orally with 50% alcohol 12 ml/kg at 4 h after the doses of ZYQL everyday for fourteen consecutive days except mice in normal group. The protective effect was evaluated by biochemical parameters including serum aspartate transaminase (AST), alanine transferase (ALT), total-bilirubin (TBIL) and reduced glutathione (GSH), malondialdehyde (MDA), superoxide dismutase (SOD) in liver tissue. The result were confirmed histopathologically and the expression of TNF-α in mice liver was determined by immunohistochemistry analysis. HPLC-PDA was used for phytochemical analysis of ZYQL, and the plant source of each compound was claritied by UPLC-TOF-MS. The result showed that pretreatment with ZYQL exhibited a significant protective effect by reversing the biochemical parameters and histopathological changes in a dose depended manner. HPLC analysis indicated that ZYQL contained flavonoids, iridoids, terpenoids and phenolic acids, which might be the active chemicals. This study demonstrated the hepatoprotective activity of ZYQL, thus scientifically supported the function of its health care.

20.
J Pharm Biomed Anal ; 71: 162-7, 2012 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-22910108

RESUMO

FR429 is an ellagitannin with a potential antitumor activity, isolated and purified from Polygonum capitatum Buch.-Ham.ex D.Don, which is a traditional Miao-nationality herbal medicine in Guizhou and Yunnan of China. Our preliminary result of pharmacology study has indicated that the antitumor activity of FR429. However, the metabolism of FR429 has not been reported yet. In this study, LC-ion trap-time of flight mass spectrometry (LC-IT-TOF/MS) was used to characterize unpredictable metabolites of FR429 biotransformed by intestinal bacteria in vitro. Total thirteen metabolites were detected and characterized via comparisons of their accurate molecular masses and fragment ions of each MS(n) stage with those of the parent drug, and four of them were also elucidated by NMR. The results demonstrated that FR429 could be transformed by intestinal bacteria in vitro, mainly via hydrolysis and reduction reaction. This work provided a basis for the further study on the biotansformation of FR429 in vivo.


Assuntos
Antineoplásicos/química , Antineoplásicos/farmacocinética , Bactérias/metabolismo , Cromatografia Líquida/métodos , Glucosídeos/química , Glucosídeos/farmacocinética , Taninos Hidrolisáveis/química , Taninos Hidrolisáveis/farmacocinética , Mucosa Intestinal/metabolismo , Intestinos/microbiologia , Animais , Biotransformação , Medicina Herbária , Hidrólise , Espectroscopia de Ressonância Magnética/métodos , Medicina Tradicional Chinesa , Ratos , Ratos Sprague-Dawley
SELEÇÃO DE REFERÊNCIAS
Detalhe da pesquisa