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1.
Int J Mol Sci ; 25(9)2024 May 02.
Artigo em Inglês | MEDLINE | ID: mdl-38732184

RESUMO

Today, allergies have become a serious problem. PR-10 proteins are clinically relevant allergens that have the ability to bind hydrophobic ligands, which can significantly increase their allergenicity potential. It has been recently shown that not only the birch pollen allergen Bet v 1 but also the alder pollen allergen Aln g 1, might act as a true sensitizer of the immune system. The current investigation is aimed at the further study of the allergenic and structural features of Aln g 1. By using qPCR, we showed that Aln g 1 was able to upregulate alarmins in epithelial cells, playing an important role in sensitization. With the use of CD-spectroscopy and ELISA assays with the sera of allergic patients, we demonstrated that Aln g 1 did not completely restore its structure after thermal denaturation, which led to a decrease in its IgE-binding capacity. Using site-directed mutagenesis, we revealed that the replacement of two residues (Asp27 and Leu30) in the structure of Aln g 1 led to a decrease in its ability to bind to both IgE from sera of allergic patients and lipid ligands. The obtained data open a prospect for the development of hypoallergenic variants of the major alder allergen Aln g 1 for allergen-specific immunotherapy.


Assuntos
Alérgenos , Antígenos de Plantas , Imunoglobulina E , Proteínas de Plantas , Pólen , Humanos , Pólen/imunologia , Pólen/química , Alérgenos/imunologia , Alérgenos/química , Antígenos de Plantas/imunologia , Antígenos de Plantas/química , Imunoglobulina E/imunologia , Proteínas de Plantas/imunologia , Proteínas de Plantas/química , Alnus/imunologia , Alnus/química
2.
J Proteome Res ; 18(5): 2354-2358, 2019 05 03.
Artigo em Inglês | MEDLINE | ID: mdl-30983355

RESUMO

Accurate target-decoy-based false discovery rate (FDR) control of peptide identification from tandem mass-spectrometry data relies on an important but often neglected assumption that incorrect spectrum annotations are equally likely to receive either target or decoy peptides. Here we argue that this assumption is often violated in practice, even by popular methods. Preference can be given to target peptides by biased scoring functions, which result in liberal FDR estimations, or to decoy peptides by correlated spectra, which result in conservative estimations.


Assuntos
Artefatos , Peptídeos/isolamento & purificação , Proteômica/normas , Espectrometria de Massas em Tandem/normas , Sequência de Aminoácidos , Viés , Humanos , Plasmodium falciparum/química , Proteômica/métodos , Espectrometria de Massas em Tandem/métodos
3.
Genome Announc ; 2(3)2014 Jun 12.
Artigo em Inglês | MEDLINE | ID: mdl-24926064

RESUMO

Here, we present a draft genome sequence of Bacillus pumilus strain 7P. This strain was isolated from soil as an extracellular RNase-producing microorganism. The RNase of B. pumilus 7P is considered to be a potential antiviral and therapeutic antitumor agent, and it might be appropriate for agriculture and academic synthesis of oligoribonucleotides.

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