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Aim: The aim of the study was to evaluate and compare the release of calcium ion, pH change, and dentinal penetration depth of four different formulations of calcium hydroxide-based intracanal medicaments. Materials and Methods: Eighty mandibular single-rooted premolar teeth were divided into four groups (n = 20): Group 1 - calcium hydroxide (CH) + distilled water (DW), Group 2 - nanocalcium hydroxide + DW, Group 3 - calcium hydroxide + chitosan, and Group 4 - calcium hydroxide + 2% chlorhexidine gluconate. Biomechanical preparation was done till the F2 rotary ProTaper system and intracanal medicaments were placed. Calcium ions and pH were assessed at 24 h, 7 days, 15 days, and 30 days using an ultraviolet spectrophotometer and pH meter, respectively. The evaluation of tubule penetration was scanned under a field emission scanning electron microscope. Results: A significant difference was seen in calcium ion release and pH change among the four groups at 24 h, 7 days, 15 days, and 30 days as well as depth of dentinal penetration. Conclusion: Calcium hydroxide mixed with 2% chlorhexidine gel showed alkaline pH and the highest calcium ion release as well as significant dentinal tubule penetration among all the four groups under observation. Both combinations can enhance antimicrobial effectiveness as intracanal medicaments. Further clinical study should be carried out to optimize its use as an alternative treatment modality.
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Objectives An ideal restorative material should be capable of supplanting the biological, functional, and aesthetic qualities of a healthy tooth structure. There has always been a search for optimal and aesthetically pleasing restorative materials. This study aims to evaluate the surface roughness and colour stability of three nanohybrid composite resins post-exposure to mouth rinse and colouring beverages. Methods One hundred and twenty specimens of dimension 10 mm x 8 mm x 1 mm were randomly allocated into three equal-sized groups and fabricated using three different nanohybrid composites (Group A: Filtek Z250 XT, Group B: Tetric N-Ceram, and Group C: Solare Sculpt). Sixty samples, comprising 20 from each group, were examined for colour stability and 60 for surface roughness after exposure to chlorhexidine and coffee. Baseline and post-exposure readings of the surface roughness and colour absorbance of the specimens were obtained by atomic force microscopy and spectrophotometer, respectively. A one-way analysis of variance (ANOVA) test followed by a post hoc Tukey's test and an independent t-test were used for data analysis, considering a p-value<0.05 as significant. Results Irrespective of the composite, the surface roughness and colour change were substantially higher in the samples exposed to coffee (p-value<0.01). Filtek Z 250XT showed significantly minor changes in colour and surface roughness, followed by Solare Sculpt and Tetric N-Ceram (p-value<0.05). Conclusion Coffee caused more surface roughness and colour changes compared to chlorhexidine. Filtek 250 XT showed minor changes in colour and surface roughness on exposure to both solutions.
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Aim: To find out the effects of 2% chlorhexidine (CHX) gel intracanal medicament on the dislodgement resistance of AH Plus, BioRoot RCS, and GuttaFlow 2 Sealer to dentin and on sealer-dentin interface. Materials and Methods: Sixty single-rooted maxillary canine were taken and divided into two groups based on the treatment: Group 1 - control group (no medicament) (n = 30) and Group 2 - (GLUCO-CHeX 2% Gel) (n = 30). Further, the groups were divided according to the sealer used, namely (A) AH Plus sealer, (B) BioRoot RCS Sealer, and (c) Gutta Flow 2 sealer. Teeth were prepared using rotary instrumentation of file size 35 with a taper of 0.06 and obturation was done. 1 mm thick sections were obtained from the roots. All the sections were checked for push out bond strength using Universal testing machine and mode of failure using stereomicroscope. Some samples were evaluated for sealer-dentin interface using field emission scanning electron microscope. Results: AH Plus showed higher bond strength compared to BioRoot RCS and GuttaFlow 2. There was no significant difference in the push out bond strength with or without the prior use of 2% CHX medicament (P > 0.05) and also CHX did not affect the mode of failure. However, sealer-dentin interface gap was increased with application of medicament. Conclusion: Dislodgement resistance of AH Plus sealer is significantly higher than BioRoot RCS and GuttaFlow 2 sealer. The application of 2% CHX intracanal medicament before root canal obturation did not significantly affect the dislodgement resistance of the three tested sealers. 2% CHX medicament did not affect the failure mode of the sealers. Sealer-dentin interface is affected by the use of 2% CHX gel medicament.
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OBJECTIVES: This study evaluated and compared the effects of radiation therapy on the dislocation resistance of AH Plus and BioRoot RCS applied to dentin and the sealer-dentin interface. MATERIALS AND METHODS: Thirty single-rooted teeth were randomly assigned to 2 groups (n = 15 each): AH Plus (Dentsply DeTrey) and BioRoot RCS (Septodont). Each group was subdivided into control and experimental groups. The experimental group was subjected to a total radiation dose of 60 Gy. The root canals of all samples were cleaned, shaped, and obturated using the single-cone technique. Dentin slices (1 mm) were sectioned from each root third for the push-out test and scanning electron microscopy (SEM) was done to examine the sealer-dentin interface. The failure mode was determined using stereomicroscopy. Bond strength data were analyzed by the independent t-test, 1-way analysis of variance, and the Tukey post hoc test (α = 0.05). RESULTS: Significantly lower bond strength was observed in irradiated teeth than non-irradiated teeth in the AH Plus group (p < 0.05). The BioRoot RCS group showed no significant reduction in bond strength after irradiation (p > 0.05) and showed a higher post-irradiation bond strength (209.92 ± 172.26 MPa) than the AH Plus group. SEM revealed slightly larger gap-containing regions in irradiated specimens from both groups. CONCLUSIONS: The dislocation resistance of BioRoot RCS was not significantly changed by irradiation and was higher than that of AH Plus. BioRoot RCS may be the sealer of choice for root canal treatment in patients undergoing radiation therapy.
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BACKGROUND: Enterococcus faecalis is the most persistent organism in the root canal which resists most of the intracanal medicaments. There is always a constant attempt to eliminate this endodontic pathogen from the root canal system. AIM: The aim of this study was to evaluate the efficacy of the association of different concentrations of proton-pump inhibitor (PPI) (Lansoprazole) with calcium hydroxide (CH) and chlorhexidine (CHX) against E. faecalis using a broth dilution method. MATERIALS AND METHODS: E. faecalis was inoculated into brain-heart infusion broth at 37°C for 5 h. The master broth was then treated with CH (Group 1); CH + 2% CHX (Group 2); CH + PPI 6.25 µg/ml (Group 3A); CH + PPI 25 µg/ml (Group 3B); 2% CHX + PPI 6.25 µg/ml (Group 4A); 2% CHX + PPI 25 µg/ml (Group 4B); CH + 2% CHX + PPI 6.25 µg/ml (Group 5A), and CH + 2% CHX + PPI 25 µg/ml (Group 5B). The groups were spectrophotometrically analyzed at 630 nm at 24 h to determine the group with the least optical density. STATISTICAL ANALYSIS: Comparison between the groups was done by the one-way analysis of variance and Kruskal-Wallis test for multiple comparisons. RESULTS: The mean percentage inhibition of E. faecalis by Group 5A (CH + 2% CHX + PPI 6.25 µg/ml) was the highest compared to other groups. The lowest mean value was observed in Group 3A (CH + PPI 6.25 µg/ml) indicating least efficiency. CONCLUSION: There was a concentration-dependent effect of PPI on CH and CHX against E. faecalis. The maximum efficacy was found when the lower concentration of PPI was associated with CH/CHX mixture.