The co-existence of anammox genera in an expanded granular sludge bed reactor with biomass carriers for nitrogen removal.

Anaerobic ammonium oxidation (anammox)-based nitrogen removal saves aeration energy and organic carbon costs, attributed to its anaerobic and autotrophic nature. However, due to the slow growth of anaerobic ammonium oxidation bacteria (AnAOB), drawbacks including long startup time and sensitivity to toxins still hamper the application of anammox-based processes. To cope with the slow growth of AnAOB, various bioreactor configurations have been investigated for the capability of retaining anammox biomass, among which, the expanded granular sludge bed (EGSB) reactor is a promising option. In this study, two laboratory-scale EGSB reactors were used to gain insights of microbial population and their response to amending biofilm-carriers, aiming to enhance the biomass retention of AnAOB. The respective ammonium and nitrite removal efficiencies were up to over 90%, and the overall nitrogen removal efficiency (NRE) was stable at over 70%, in the EGSB reactor amended with carriers (CEGSB). Compared to the control EGSB, CEGSB's observed performance was more stable during the 236-day operational period. The abundance of AnAOB reached 22% in the EGSB and 49% in the CEGSB. It was also observed that Ca. Brocadia (14.25%) and Asahi BRW2 (33.19%) coexisted in the CEGSB. The dynamics of major metabolisms and functional genes involved in nitrogen conversion were further observed by FAPROTAX based on the taxonomic data, providing more insights into the functions of the microbial communities.

Development of radio-frequency identification (RFID) sensors suitable for smart-monitoring applications in sewer systems.

Sanitary and stormwater sewers are buried assets that play important roles in the prevention of diseases and the reduction of health risks for our societies. Due to their hidden nature, these assets are not frequently assessed and maintained to optimal conditions. The lack of maintenance can cause sewer blockages and overflows that result in the release of pathogens into the environment. For cities, monitoring sewer conditions on a large-scale can be costly, time-consuming, and labor-intensive if using current low-throughput technologies, such as dye testing or closed-circuit television. Alternatively, smart sensor systems can provide low-cost, high-throughput, and automatic data-driven features for real-time monitoring applications. In this study, we developed ultrahigh-frequency radio-frequency identification (UHF RFID)-based sensors that are flushable and suitable for sanitary and stormwater pipes quick surveys. 3D printed RFID sensors were designed to float at the water-air interface and minimize the water interference to RF signal communications. The optimal detection range was also determined to support the design and installation of the reader in various utility holes. Field trials demonstrated that the UHF RFID system is a low-cost, high-throughput, and robust solution for monitoring blockage, illicit-connection, and water flow in sewer networks.

The Responses to Long-Term Water Addition of Soil Bacterial, Archaeal, and Fungal Communities in A Desert Ecosystem.

The response of microbial communities to continual and prolonged water exposure provides useful insight when facing global climate changes that cause increased and uneven precipitation and extreme rainfall events. In this study, we investigated an in situ manipulative experiment with four levels of water exposure (ambient precipitation +0%, +25%, +50%, and +100% of local annual mean precipitation) in a desert ecosystem of China. After 9 years of water addition, Illumina sequencing was used to analyze taxonomic compositions of the soil bacterial, archaeal, and fungal communities. The results showed significant increases in microbial biomass carbon (MBC) at higher amended precipitation levels, with the highest values reported at 100% precipitation. Furthermore, an increase in the bacterial species richness was observed along the water addition gradient. In addition, the relative abundance of several bacterial phyla, such as Proteobacteria, significantly increased, whereas that of some drought-tolerant taxa, including Actinobacteria, Firmicutes, and Bacteroidetes, decreased. In addition, the phyla Planctomycetes and Nitrospirae, associated with nitrification, positively responded to increased precipitation. Archaeal diversity significantly reduced under 100% treatment, with changes in the relative abundance of Thaumarchaeota and Euryarchaeota being the main contributors to shifts in the archaeal community. The fungal community composition was stable in response to water addition. Results from the Mantel test and structural equation models suggested that bacterial and archaeal communities reacted contrastingly to water addition. Bacterial community composition was directly affected by changing soil moisture and temperature, while archaeal community composition was indirectly affected by changing nitrogen availability. These findings highlight the importance of soil moisture and nitrogen in driving microbial responses to long-term precipitation changes in the desert ecosystem.

Assessing inorganic components of cake layer in A/O membrane bioreactor for physical-chemical treated tannery effluent.

In this study, an anoxic-oxic membrane bioreactor (A/O-MBR), was used to treat effluent tannery wastewater pretreated by physicochemical processes. The A/O-MBR performed well during the experimental period and was able to produce a high-quality effluent containing 90 ± 10 mg-CODcr/L and 0.5 ± 0.1 mg-NH4+-N/L. However, it was observed that at rates of approximately 1.02 kPa/day and 1.2 µm/day, both transmembrane pressure (TMP) and thickness of cake layer increased during wastewater treatment. The eventual thickness of the cake layer was between 47.8 and 51.5 µm. Furthermore, an Inductively Coupled Plasma-Optical Emission Spectrometer, used to analyze inorganic components of the cake layer, revealed that four inorganic elements, Cr, Ca, Mg and Al were predominant (weight percentage rate 4:13:10:72). Due to low solubility (Cr(OH)3: Ksp 6.3 × 10-31; Al(OH)3: Ksp 6.3 × 10-19), the elements of Cr and Al mainly existed in the forms of Cr(OH)3 and Al(OH)3, respectively. Other minerals in the cake layer included Al2O3, CaCO3, and MgCO3. Additionally, using an SEM-EDX (Scanning electron microscopy-energy dispersive X-ray analyzer), we discovered that inorganic particles that formed within the activated sludge of the A/O-MBR steadily accumulating on the membrane surface, resulted in an evenly distributed inorganic layer which could be observed along the cross-sections of the cake layer.

Microbial community in in-situ waste sludge anaerobic digestion with alkalization for enhancement of nutrient recovery and energy generation.

Microbial community in in-situ waste sludge anaerobic digestion with alkalization for enhancement of nutrient recovery and energy generation was studied. Firmicutes, Proteobacteria and Bacteroidetes phylum became the majority in the microbial community, especially Firmicutes showed the predominate role in the community due to its thick cell wall structure, potential ability hydrolysis and hydrogenogenic acidogenesis. Anaerobic digestion with alkalization caused the obvious microbial diversity decrease, and over 50% of minority bacteria grew up in quantity from original sludge. Phylum of Firmicutes developed by themselves having few interactions with other bacteria, partly contributing to its rapid growth in anaerobic digestion with alkalization. The decrease of hydrocarbon degradation, and the increase of both fermentation and reductive acetogenesis in microbial community, indicating the promotion of short chain fatty acids production, especially acetic acid which is the key intermediate products for nutrient recovery and energy generation.