RESUMO
BACKGROUND: Rhabdomyosarcoma is the most common soft tissue sarcoma of children < 15 years of age. CASE: We report a case of a paratesticular rhabdomyosarcoma diagnosed by fine needle aspiration (FNA) in an 18-year-old man with enlargement of the right testis. The FNA material revealed mainly isolated small to median-sized malignant cells with various shapes. Among these, large rhabdomyoblasts were observed. Some neoplastic cells showed longitudinal striations. Immunocytochemistry was strongly positive against vimentin and desmin, and results for cytokeratins, smooth muscle antibody, epithelial-membrane antigen, S-100 and neuron-specific enolase were negative. Histologic examination of the surgical specimen was in agreement with the FNA findings. CONCLUSION: FNA is a safe and accurate procedure, without implications, that could be used in the outpatient setting, allowing a preliminary, preoperative diagnosis, even for soft tissue tumors.
Assuntos
Rabdomiossarcoma/patologia , Neoplasias Testiculares/patologia , Testículo/patologia , Adolescente , Biomarcadores Tumorais/análise , Biópsia por Agulha Fina , Desmina/análise , Humanos , Imuno-Histoquímica , Masculino , Rabdomiossarcoma/química , Rabdomiossarcoma/cirurgia , Neoplasias Testiculares/química , Neoplasias Testiculares/cirurgia , Testículo/química , Vimentina/análiseRESUMO
We aimed to evaluate the feasibility and reliability of brush cytology in the biomarker expression profiling of oral squamous cell carcinomas within the concept of theranostics, and to correlate this biomarker profile with patient measurable outcomes. Markers representative of prognostic gene expression changes in oral squamous cell carcinoma was selected. These markers were also selected to involve pathways for which commercially available or investigational agents exist for clinical application. A set of 7 markers were analysed by immunocytochemistry on the archival primary tumour material of 99 oral squamous cell carcinoma patients. We confirmed the feasibility of the technique for the expression profiling of oral squamous cell carcinomas. Furthermore, our results affirm the prognostic significance of the epidermal growth factor receptor (EGFR) family and the angiogenic pathway in oral squamous cell carcinoma, confirming their interest for targeted therapy. Brush cytology appears feasible and applicable for the expression profiling of oral squamous cell carcinoma within the concept of theranostics, according to sample availability.
Assuntos
Biomarcadores Tumorais/genética , Carcinoma de Células Escamosas/diagnóstico , Citodiagnóstico/métodos , Perfilação da Expressão Gênica , Neoplasias Bucais/diagnóstico , Nanomedicina Teranóstica , Idoso , Idoso de 80 Anos ou mais , Biópsia , Carcinoma de Células Escamosas/genética , Feminino , Seguimentos , Humanos , Técnicas Imunoenzimáticas , Masculino , Pessoa de Meia-Idade , Neoplasias Bucais/genética , Gradação de Tumores , Estadiamento de Neoplasias , Projetos Piloto , Prognóstico , RNA Mensageiro/genética , Reação em Cadeia da Polimerase em Tempo Real , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Taxa de SobrevidaRESUMO
Leydig cell tumors (LCT) are rare sex cord-stromal tumors that account for 2-3% of all testicular tumors. Approximately 10% of LCTs shows evidence of malignant behavior. We present a case of LCT with severe atypia diagnosed by fine-needle aspiration (FNA) in a 49-year-old man who presented with gynecomastia and right testis enlargement. The FNA material on conventional and ThinPrep smears revealed a hemorrhagic and necrotic background with high cellularity, consisting of large cells, isolated or in small cohesive clusters, abundant, eosinophilic cytoplasm, round nuclei, fine chromatin, and variably conspicuous nucleoli. Occasionally, pleomorphic cells with hyperchromatic nuclei and prominent nucleoli were seen. Immunocytochemistry was positive against vimentin, inhibin, and calretinin. Histological examination of the surgical specimen was in accordance with the FNA findings. The cytologic diagnosis of LCT of the testis, using FNA, is achievable in a preoperative setting to vitiate the need for more invasive biopsy procedures; malignancy could be considered on cytology when necrosis and marked atypia are evident.