Evaluation of the long-term results of rat cranial bone repair using a particular xenograft.

Bone defects that cannot be healed completely are termed critical-sized defects and can be used to test bone grafts for medicine, dentistry, and periodontology. The aim of the present study was to detect the effects of a xenograft (Unilab Surgibone) on bone building in experimentally created parietal bone defects in rats. Standardized parietal bone defects were created in 16 rats, and each defect had a circular morphology 6 mm in diameter. The right defect sites were filled with porous particle material, and the left site was used as control. After the 3rd, 6th, and 12th months, rats were killed and tissue samples obtained from the related site of the cranium. Subsequently, histological sections were taken and stained with different stains for evaluation under light microscope. The rate of bone formation was assessed using a semiquantitative method. These results showed that dense collagenous tissue was observed in the control area during the third month, whereas xenograft particles were surrounded by a fibrous tissue layer at the implantation site. Osteoclast-like cells were also observed. There was also no significant bone repair at other observation periods. It can be concluded that the material used had no evidence of resorption and does not enhance bone formation. However, it seems biocompatible, osteoconductive, and could be used in a limited manner as a material for filling osseous defects in clinical practice.

Investigation of the levels of different salivary stress markers in chronic periodontitis patients.

BACKGROUND AND AIM: In this study, we aimed to determine the salivary levels of cortisol, α-amylase, ß-endorphin, and chromogranin (CgA) in saliva and to investigate their relationship with periodontitis. METHODS: Thirty-seven female and 43 male periodontitis patients who presented to the Periodontology Department of the XXX University Faculty of Dentistry participated in the study. The individuals were divided into three groups: mild, moderate, or severe chronic periodontitis. Plaque index (PI), gingival index (GI), clinical attachment loss (CAL), and probing depth (PD) measurements were recorded for all the participants. All participants underwent the State-Trait Anxiety Inventory test (STAI 1 and 2). Between 09:00 and 11:00 a.m., saliva samples from the participants were collected into tubes within an average of five minutes. RESULTS: Higher cortisol measurements were detected in the saliva samples of participants with severe chronic periodontitis than in those who had mild chronic periodontitis (P < 0.05). There were statistically significant age differences among patients with mild-moderate, moderate-severe, and mild chronic periodontitis, the severity of the disease increasing with age (P < 0.05). There was also a positive correlation between STAI 1 stress scores and cortisol levels. Similarly, there was a positive correlation between CAL and cortisol levels (P < 0.05). However, a significant difference was found among groups only in terms of salivary cortisol levels (P < 0.05). CONCLUSION: Within the limitations of this study, there was found to be a relationship between saliva cortisol levels and periodontitis and between salivary cortisol levels and stress.

Assessment of the outcomes of cerebral blood flow measurements after electrical stimulation of upper right incisor tooth in rabbits.

The cerebral vessels are innervated by sympathetic, parasympathetic, and sensory nerves. A sensory innervation of the cerebral vessels originating in the trigeminal ganglion has been described in a number of species by several investigations. It has been shown that the electrical stimulation of the trigeminal ganglion causes an increase of cerebral cortical blood flow (CCoBF). The aim of the present study was to determine the effects of dental electrical stimulation the CCoBF in rabbits. A stimulating electrode was located in the upper right incisor tooth of rabbits and trigeminal ganglion was stimulated orthodromically via the infraorbital nerve. Variations in the cortical CCoBF were evaluated by laser-Doppler flowmetry. In experiment group, CCoBF increased together with the beginning of electrical stimulation (5 V, 0.5-ms impulse duration, square-shaped, 10-Hz frequency). The right and left hemisphere CCoBF values of stimulation period at 15s, 30s, 45s, 60s, 75s, and 90s were significantly higher than those of baseline and 105 and 120s (p < 0.05). The maximum increase in right and left CCoBF was 15.6% and 15.1% respectively. In post-stimulation period, the right CCoBF decreased gradually and returned to the baseline values at 120 s. In experiment groups, the CCoBF values of right hemisphere were comparable that of left hemisphereL (p > 0.05). This study demonstrated that the electrical stimulation of the trigeminal nerve's infraorbital branch via dental pulp increases the cortical right and left CCoBF under physiological conditions.

The bone-healing effect of a xenograft in a rat calvarial defect model.

Bone grafts have been widely used to fill osseous defects in medicine, dentistry, and periodontology. The purpose of this study was to investigate the effects of a xenograft (Unilab Surgibone) on experimentally created parietal bone defects in rats. To this end, 14 rats were employed in the present study and in each of them, 5-mm-diameter defects were created on the parietal bone. The right defect sites were filled with the xenograft material, while the left sites were used as control. After 30 days, the rats were sacrificed and tissue samples were retrieved from the defect sites of the cranium. Dense collagenous tissue was observed in the control area, whereas the xenograft particles were surrounded by a fibrous tissue layer at the implantation site. Based on the findings obtained, it could be concluded that the investigated xenograft seemed biocompatible and could be proposed as a potential material for filling osseous defects.

Histological findings of long-term healing of the experimental defects by application of a synthetic biphasic ceramic in rats.

Calcium phosphate ceramics are generally biocompatible and can develop interactions with human recipient bone. Therefore, they can be widely used in the field of periodontology and dentistry. The purpose of this investigation was to assess the long-term histological bone healing results of experimentally created critical size parietal bone defects in rats. Twelve Wistar rats were used in this investigation. Two 6-mm wide, symmetrical, and circular critical size defects were created in each parietal bone of the animals. While the right defects filled with granular implant (Ceraform), the symmetrical defects were taken as controls. Eighteen months after implantation, rats were killed and defects including the biomaterial with surrounding bone was taken for histological examination. Serial histological sections were cut across the defects and stained for the histological analysis. Both control and Ceraform implanted regions contained dense collagenous tissue. In the implantation site, multinuclear giant cells were observed around the material. On the other hand, there were no necrosis, tumour, and infection in the implantation region. There was no statistical difference between the control and ceraform implanted groups when the bone formation results were compared (p > 0.05). In conclusion, the results revealed that this material is biocompatible and does enhance the new bone building despite the long-term observation period. Although this biphasic ceramic shows within the limits of the study as a less resorptive and not osteoconductive properties, it can be considered as a biocompatible bone defect filling material having a limited application alternative in dentistry and medicine.

Assessment of the effect of a biphasic ceramic on bone response in a rat calvarial defect model.

Calcium phosphate ceramics are being extensively used for orthopedic, periodontal, and dental applications. This study aimed to assess the effect of a biphasic ceramic such as Ceraform on the osteogenesis in a rat calvarial defect model. 20 Wistar rats were enrolled in the study. Two symmetrical, circular, and 5-mm-wide full thickness defects were created in the parietal bones of each animal. The left defect was left empty as a control and the right defect was filled with the particular implant material. Animals were divided into two groups, and 10 animals were sacrificed at month 3 and the rest were sacrificed at month 6. The calvarial specimens were harvested for histological examinations. Defect area samples were stained with hematoxylin-eosin and Masson Thrichrom. A semiquantitative method was used to quantify the bone regeneration. The defects were mostly filled with fibrous connective tissue (3-6 months) in the control site. A loose, fibrovascular tissue was observed at the side of ceraform implantation at month 3. By 6 month, a dense collagenous tissue was observed at the same area. Multinuclear giant cells (MNGC) were detected around the implant bed at month 3 and month 6. No necrosis, tumorigenesis, or infection was observed at the implantation site at any time. There was no statistically meaningful difference regarding bone regeneration between the two defects at each observation period (p>0.05). This study showed that Ceraform is biocompatible. However, this study indicates that biphasic ceramic do not offer any advantage over hydroxyapatite ceramics. It was also revealed that it had no effects on bone regeneration and that it seemed to be a space maintainer.

Evaluation of the marginal gingival health using laser Doppler flowmetry.

The purposes of this study were to compare the gingival blood flow (GBF) in test sites (teeth retaining fixed partial dentures) and control sites (contralateral natural teeth) and investigate whether there is any relationship between clinical indices and GBF values. Twelve healthy subjects (6 females and 6 males) aged 20 to 54 years were enrolled this study. The GBF was measured from the middle point of the marginal gingiva in the test and control sites using laser Doppler flowmetry (LDF). Additionally, plaque index, gingival index and probing depth measurements were recorded. Statistically significant difference (p<0.05) was found between the test and control sites for marginal GBF. In contrast, no significant difference (p>0.05) was found between test and control sites with respect to the clinical indices, except for plaque index. The findings of this study suggest that there is a significant relation between resin-bonded fixed partial dentures with margins located subgingivally and marginal GBF. Clinical indices are helpful to collect information about the clinical health status of gingival tissues, but GBF is a good tool to measure gingival tissue blood flow and assess periodontal health. In conclusion, laser Doppler flowmetry can be used together with clinical indices to evaluate the marginal gingival health.

The effect of a biphasic ceramic on calvarial bone regeneration in rats.

Bioactive ceramics (calcium phosphate ceramics, hydroxyapatite ceramics) are now extensively used in oral surgery. The purpose of this study was to assess the effect of a new biphasic ceramic (Ceraform) on the osteogenesis in a rat calvarial defect model. Fifteen Wistar rats were used in this study. Two symmetrical 3-mm wide defects were created in the skull of each rat. The left defect was left empty as a control and the right defect was filled with the ceramic. The rats were sacrificed at day 30, and the calvarial specimens were processed for qualitative and quantitative histological examinations. The material exhibited no adverse effects, but no bone healing was noted either. No statistical difference regarding bone regeneration was observed between the 2 defects (P > .05). This study showed that Ceraform did not elicit any inflammatory reaction; however, it had no effect on bone regeneration, and this material seems suitable only as a space-maintaining material.

Lymphoma in the infraorbital region.

BACKGROUND: B-cell lymphomas are rare neoplasms in the oral cavity. They are significant to dentists because the oral complications associated with treatment mean that dentists can play an important role in their detection. CASE DESCRIPTION: The authors describe the case of a 55-year-old man with nonhealing ulcers, swelling and pain six months after maxillary left canine extraction. As it occurred in the infraorbital region after canine tooth extraction, the authors suspected B-cell lymphoma. Clinical examination revealed infraorbital edema with regional submandibular lymphadenopathy. Intraoral examination revealed a nonhealing ulceration with ill-defined borders in the surrounding mucosa. It was 5- x 5-centimeters in diameter and gray-white. The histopathologic examination showed diffuse, atypical, lymphoid cell infiltration and immuno-histochemically positive staining. After the histopathologic examination, the authors referred the patient to a medical center for treatment. CLINICAL IMPLICATIONS: Dentists should look for signs of B-cell lymphoma when a patient has extended pain and swelling after an extraction.

The adjunctive effect of tenoxicam during non-surgical periodontal treatment on clinical parameters and gingival crevicular fluid levels of MMP-8 and TNF-α in patients with chronic periodontitis - randomized, double-blind clinical trial.

BACKGROUND: Matrix metalloproteinases (MMPs) and cytokines play a role of extracellular matrix degradation and remodelling, and are significantly involved in the course of periodontal disease. OBJECTIVES: The purpose of this study was to evaluate the adjunctive effect of administering an oxicam non-steroidal anti-inflammatory drug (NSAID), tenoxicam, during non-surgical (phase 1) periodontal treatment on clinical parameters and gingival crevicular fluid (GCF) levels of MMP-8 and TNF-α in subjects with chronic periodontitis. MATERIAL AND METHODS: A total of 32 subjects with chronic periodontitis were randomized into two groups: 1) phase I periodontal treatment + NSAID and 2) phase I periodontal treatment + placebo. Phase I periodontal therapy consisted of scaling and root planning (SPR), which was provided by a single therapist masked with respect to group assignment. Patients in group 1 received a systemic NSAID (20 mg tenoxicam tablet once daily for 10 days). Clinical measures and GCF samples were obtained immediately prior to periodontal treatment and 30 days afterwards from all subjects. Clinical measures included a plaque index, gingival index, gingival bleeding time index, probing depth, and clinical attachment level. The MMP-8 and TNF-α levels in the GCF were assayed using an enzyme-linked immunosorbent assay. RESULTS: With the exception of clinical attachment level, all clinical measures showed a significant (p < 0.05) improvement following non-surgical treatment in both the NSAID and placebo groups. A significant decrease in MMP-8 levels (p < 0.05) was observed at post-treatment in the NSAID group but not in the placebo group (p > 0.05). Treatment exhibited no effect on TNF-α levels (p > 0.05). There was also no statistically significant difference in clinical measurements after treatment between the two groups (p > 0.05). Moreover, the post treatment MMP-8 level in group 1 was statistically significant higher than the placebo group (p < 0.05). CONCLUSIONS: The adjunctive administration of tenoxicam during phase I periodontal treatment decreases MMP-8 levels in gingival crevicular fluid in patients with chronic periodontitis. But no benefits were observed.

Larsen's syndrome with dental anomalies: report of a case.

Dental anomalies and mixed-type hearing loss are extremely rare symptoms of Larsen's syndrome that is characterized by multiple joint dislocation and flat face. A 15-year-old Turkish girl affected with Larsen's syndrome exhibiting many characteristic facial and skeletal features plus hitherto unreported oral and oral-facial anomalies including, maxillary prognathi, malocclusion, supernumerary teeth, macroglossia and microdontia is reported.

Evaluation of the marginal gingival health using laser doppler flowmetry

The purposes of this study were to compare the gingival blood flow (GBF) in test sites (teeth retaining fixed partial dentures) and control sites (contralateral natural teeth) and investigate whether there is any relationship between clinical indices and GBF values. Twelve healthy subjects (6 females and 6 males) aged 20 to 54 years were enrolled this study. The GBF was measured from the middle point of the marginal gingiva in the test and control sites using laser Doppler flowmetry (LDF). Additionally, plaque index, gingival index and probing depth measurements were recorded. Statistically significant difference (p<0.05) was found between the test and control sites for marginal GBF. In contrast, no significant difference (p>0.05) was found between test and control sites with respect to the clinical indices, except for plaque index. The findings of this study suggest that there is a significant relation between resin-bonded fixed partial dentures with margins located subgingivally and marginal GBF. Clinical indices are helpful to collect information about the clinical health status of gingival tissues, but GBF is a good tool to measure gingival tissue blood flow and assess periodontal health. In conclusion, laser Doppler flowmetry can be used together with clinical indices to evaluate the marginal gingival health.

Os objetivos deste estudo foram comparar o fluxo sangüíneo gengival (FSG) em sítios teste (dentes retentores de próteses parciais fixas) e sítios controle (dentes naturais contralaterais) e investigar se há alguma relação entre os índices clínicos (IC) e os valores de FSG. Doze indivíduos saudáveis (6 mulheres e 6 homens) com idades entre 20 a 54 anos participaram deste estudo. O FSG foi medido no ponto médio da gengina marginal em ambos os sítios teste e controle utilizando dopplerfluxometria a laser (DFL). Além disso, as medidas referentes ao índice de placa, índice gengival e profundidade de sondagem foram registradas. Foi observada diferença estatisticamente significante (p<0.05) entre os grupos teste e controle para os valores de FSG. Por outro lado, não houve diferença estatisticamente significante (p>0.05) entre os sítios teste e controle com relação aos índices clínicos, exceto para o índice de placa. Os achados deste estudo sugerem que existe uma relação significante entre próteses parciais fixas retidas por resina com margens localizadas subgengivalmente e o FSG marginal. Embora os índices clínicos sejam úteis para coletar informações sobre as condições clínicas dos tecidos gengivais, o FSG é uma ferramenta importante para medir o fluxo sanguíneo tissular gengival e avaliar a saúde periodontal. Em conclusão, a dopplerfluxometria a laser pode ser usada juntamente com índices clínicos para avaliar a saúde gengival marginal.