Cardiovascular disease (CVD) in Canadians with haemophilia: Age-Related CVD in Haemophilia Epidemiological Research (ARCHER study).

INTRODUCTION: Improvements in haemophilia care have increased life expectancy in persons with haemophilia (PWH). This ageing population presents clinicians with management challenges as they develop age-related comorbidities such as cardiovascular disease (CVD). AIMS: To assess the epidemiology of CVD risk factors and events in an ageing Canadian haemophilia population. METHODS: A retrospective, multicentre chart review was carried out at five Canadian Hemophilia Treatment Centres. PWH (A and B) ≥35 years old were included and data were extracted on CVD risk factors and events. RESULTS: Data from 294 patients' charts were analysed including 222 (75.5%) patients with haemophilia A and 72 (24.5%) patients with haemophilia B with a median age at end of follow-up of 54 years (range = 36-90). Mean follow-up duration was 5.86 years. Cardiovascular risk factors were common: hypertension 31.3% (n = 90), diabetes mellitus 10.5% (n = 29), smoking 21.8% (n = 61), obesity 27.6% (n = 69), dyslipidaemia 22.4% (n = 65), family history 8.5% (n = 24), antiretroviral therapy 12.2% (n = 36). There were 24 CVD events (8.2% of the population) with a median age at event of 63 years (range = 46-83). Events consisted of coronary artery disease (CAD), 14; cerebrovascular disease, 4; and atrial fibrillation, 7. CAD was treated with coronary artery bypass grafting in three patients and percutaneous coronary intervention in nine patients. CVD events were complicated by six bleeding events (three minor and three major). CONCLUSION: Cardiovascular disease risk factors and events are relatively common in PWH. PWH can be safely treated for CVD events with similar procedures as the non-PWH populations, though specific clotting factor prophylaxis protocols are not well defined.

Time-resolved thermally induced aberrations in a flash-lamp pumped Nd:Glass disk amplifier using a 2 × 2 position sensitive detector array.

A novel technique of measuring the prompt, thermally induced wave-front aberrations in a large aperture flash-lamp pumped Nd3+ glass disk amplifier is presented. Implementing a 2 × 2 lens array and a 2 × 2 position sensitive detector array as a diagnostic system, the wave-front profile was successfully reconstructed for the first five Zernike terms for a temporal window of 8.5 ms.

Identification of functional apple scab resistance gene promoters.

Apple scab (Venturia inaequalis) is one of the most damaging diseases affecting commercial apple production. Some wild Malus species possess resistance against apple scab. One gene, HcrVf2, from a cluster of three genes derived from the wild apple Malus floribunda clone 821, has recently been shown to confer resistance to apple scab when transferred into a scab-susceptible apple variety. For this proof-of-function experiment, the use of the 35S promoter from Cauliflower mosaic virus was reliable and appropriate. However, in order to reduce the amount of non-plant DNA in genetically modified apple to a minimum, with the aim of increasing genetically modified organism acceptability, these genes would ideally be regulated by their own promoters. In this study, sequences from the promoter region of the three members of the HcrVf gene family were compared. Promoter constructs containing progressive 5' deletions were prepared and used for functional analyses. Qualitative assessment confirmed promoter activity in apple. Quantitative promoter comparison was carried out in tobacco (Nicotiana glutinosa) and led to the identification of several promoter regions with different strengths from a basal level to half the strength of the 35S promoter from Cauliflower mosaic virus.

Dermal exposure to electroplating fluids and metalworking fluids in the UK.

This paper describes workplace dermal exposure measurements that were carried out by the Health and Safety Laboratory as part of the EU RISKOFDERM project. Exposure to metalworking fluids (MWFs) was measured at three sites on 25 subjects who were 'mechanically treating solid objects' as they loaded and supervised milling and boring machines and lathes. Thirty-one samples were obtained, of which 18 were exposures to neat mineral oils and 13 to water-oil mixes. All subjects wore Tyvek whole-body oversuits that were analysed in their entirety to extract the MWF. The geometric mean surface loading rate of the 31 oversuits was 62 micro g/cm(2)/h (GSD = 4.6) and of the seven pairs of sampling gloves (worn inside protective gloves) was 2900 micro g/cm(2)/h (GSD = 1.67). Exposure to electroplating fluids was measured at three sites on 27 subjects who were dipping objects into tanks of either chromic acid, nickel sulphate, copper sulphate, copper cyanide or zinc hydroxide. All subjects wore Tyvek whole-body oversuits that were surface scanned over their areas using a portable X-ray fluorescence spectrometer to detect all the metal atoms simultaneously. Contamination was assessed using the method of Dirichlet tessellation. The geometric mean surface loading rate of the 26 oversuits was 37 micro g/cm(2)/h (GSD = 3.5) and of the 25 pairs of sampling gloves (worn inside protective gloves) was 190 micro g/cm(2)/h (GSD = 2.75). Almost all of the electroplating samples were below the limit of quantification. More than one species of metal atoms was found on some of the samples afterwards, indicating cross-contamination from other baths during the sampling period.

Dermal exposure to dry powder spray paints using PXRF and the method of Dirichlet tesselations.

This paper describes workplace dermal exposure measurements that were carried out by the Health and Safety Laboratory as part of the EU RISKOFDERM project to measure dust contamination. Exposure to dry powder spray paints was measured at five sites on 12 subjects. Twenty-two samples were obtained, of which eight contained triglycidyl isocyanurate (TGIC) and 14 did not. All subjects wore Tyvek whole body oversuits and some wore sampling gloves. These were either analysed in their entirety to extract the TGIC or surface scanned over representative areas using a portable X-ray fluorescence spectrometer (PXRF) to detect barium or titanium in the fillers of the paints. The method of Dirichlet tessellation was used to map the scans and the technique was developed further for these studies to extend measurements to gloves and to take limits of detection into consideration. The PXRF allowed dusts to be measured in situ that would otherwise be difficult to extract from the material and analyse by other means. The geometric mean surface loading rate of the 22 oversuits was 43 micro g/cm/(2)/h (GSD = 6.0) and of the 23 pairs of sampling gloves was 970 micro g/cm(2)/h (GSD = 8.6). Exposure patterns could be attributed to the arrangements of the subjects, spray booths and the workpieces. Similar exposures were found for TGIC and titanium fillers in factories with similar methods of ventilation.