Fabrication and application of carrier-free and carrier-based nanopesticides in pest management.

Pesticides are widely used for pest control to promote sustained and stable growth of agricultural production. However, indiscriminate pesticide usage poses a great threat to environmental and human health. In recent years, nanotechnology has shown the ability to increase the performance of conventional pesticides and has great potential for improving adhesion to crop foliage, solubility, stability, targeted delivery, and so forth. This review discusses two types of nanopesticides, namely, carrier-free nanopesticides and carrier-based nanopesticides, that can precisely release necessary and sufficient amounts of active ingredients. At first, the basic characterization and preparation methods of these two distinct types of nanopesticides are briefly summarized. Subsequently, current applications and future perspectives on scientific examples and strategies for promoting the usage efficacy and reducing the environmental risks of these nanopesticides were also described. Overall, nanopesticides can promote higher crop yields and lay the foundation for sustainable agriculture and global food security.

Identification and expression patterns of somatic piRNAs and PIWI genes in Riptortus pedestris (Hemiptera: Alydidae).

RNA interference (RNAi)-based gene silencing is a feasible and sustainable technology for the management of hemipteran pests by double-stranded RNA involvement, including small-interfering RNA, microRNA, and Piwi-interacting RNA (piRNA) pathways, that may help to decrease the usage of chemical insecticides. However, only a few data are available on the somatic piRNAs and their biogenesis genes in Riptortus pedestris, which serves as a significant pest of soybean (Glycine max). In this study, two family members of the PIWI gene were identified and characterized in R. pedestris, containing Argonaute3 (RpAgo3) and Aubergine (RpAub) genes with conserved protein domains, and their clusters were validated by phylogenetic analysis. In addition, they were widely expressed in all developmental stages of the whole body of R. pedestris and had lower expression levels in R. pedestris guts under different rearing conditions based on previous transcriptome sequencing. Furthermore, abundant clean reads were filtered to a total number of 45,998 piRNAs with uridine bias at the first nucleotide (nt) position and 26-32 nt in length by mapping onto the reference genome of R. pedestris according to our previous whole-transcriptome sequencing. Finally, our data revealed that gut bacterial changes were significantly positively or negatively associated with differentially expressed piRNAs among the five comparison groups with Pearson correlation analysis. In conclusion, these findings paved new avenues for the application of RNAi-based biopesticides for broad-spectrum hemipteran pest control.

Effects of albumin and crystalloid priming strategies on red blood cell transfusions in on-pump cardiac surgery: a network meta-analysis.

BACKGROUND: In on-pump cardiac surgery, the albumin priming strategy could maintain colloid osmotic pressure better than crystalloid solutions and reduce excessive perioperative fluid balance. However, a high-quality meta-analysis is required to compare the safety of these approaches in perioperative red blood cell (RBC) transfusions. Owing to limited direct evidence, we conducted a network meta-analysis (NMA) to increase the pool of studies and provide indirect evidence. METHODS: The pre-defined primary outcomes were intraoperative and the first 24 h postoperative RBC transfusion volume in units. The pre-defined secondary outcome was postoperative blood loss (the first 24 h). We reviewed all randomized controlled trials comparing albumin, crystalloid, and artificial colloid priming strategies. Studies that only displayed pre-defined outcomes could be included. A pairwise meta-analysis was performed on studies that directly compared the pre-defined outcomes between albumin and crystalloids. Additionally, a random-effects network meta-analysis (NMA) model was employed to generate indirect evidence for the pre-defined outcomes between albumin and crystalloids. RESULTS: The literature search identified 830 studies,10 of which were included in the final analysis. Direct meta-analysis indicated that crystalloid priming significantly decreased total perioperative RBC transfusions (MD: -0.68U; 95%CI: -1.26, -0.09U; P = 0.02) and intraoperative RBC transfusions (MD: -0.20U; 95%CI: -0.39, -0.01U; P = 0.03) compared to albumin. Postoperative RBC transfusions showed a decreasing trend in the crystalloid group; however, the difference was not statistically significant. (MD: -0.16U; 95%CI: -0.45, 0.14U; P = 0.30). After including indirect evidence, the NMA results continued to demonstrate a higher RBC receiving with the albumin priming strategy compared to crystalloids, although the differences did not reach statistical significance. For postoperative blood loss, direct evidence showed no significant differences between albumin and crystalloid priming strategies. However, NMA evidence displayed that albumin exist higher probability of reducing postoperative blood loss than crystalloid. CONCLUSION: Both direct and NMA evidence indicated that the albumin priming strategy resulted in more perioperative RBC transfusions than crystalloids. Considering the additional blood management burden, the application of an albumin-priming strategy in on-pump cardiac surgery still needs more consideration.

Photo-Induced Self-assembly of Copolymer-Capped Nanoparticles into Colloidal Molecules.

Colloidal molecules (CMs) are precisely defined assemblies of nanoparticles (NPs) that mimic the structure of real molecules, but externally programming the precise self-assembly of CMs is still challenging. In this work, we show that the photo-induced self-assembly of complementary copolymer-capped binary NPs can be precisely controlled to form clustered ABx or linear (AB)y CMs at high yield (x is the coordination number of NP-Bs, and y is the repeating unit number of AB clusters). Under UV light irradiation, photolabile p-methoxyphenacyl groups of copolymers on NP-A*s are converted to carboxyl groups (NP-A), which react with tertiary amines of copolymers on NP-B to trigger the directional NP bonding. The x value of ABx can be precisely controlled between 1 and 3 by varying the irradiation duration and hence the amount of carboxyl groups generated on NP-As. Moreover, when NP-A* and NP-B are irradiated after mixing, the assembly process generates AB clusters or linear (AB)y structures with alternating sequence of the binary NPs. This assembly approach offers a simple yet non-invasive way to externally regulate the formation of various CMs on demand without the need of redesigning the surface chemistry of NPs for use in drug delivery, diagnostics, optoelectronics, and plasmonic devices.

Vesicular self-assembly of copolymer-grafted nanoparticles with anisotropic shapes.

Plasmonic nanovesicles show broad applications in areas such as cancer theranostics and drug delivery, but the preparation of nanovesicles from shaped nanoparticles remains challenging. This article describes the vesicular self-assembly of shaped nanoparticles, such as gold nanocubes grafted with amphiphilic block copolymers, in selective solvents. The nanocubes assembled within the vesicular membranes exhibit two distinctive packing modes, namely square-like and hexagonal packing, depending on the relative dimensions of the copolymer ligands and nanocubes. The corresponding optical properties of the plasmonic nanovesicles can be tuned by varying the length of the grafted copolymers and the size of the nanocubes. This work provides guidance for the fabrication of functional plasmonic vesicles for applications in catalysis, nanomedicines and optical devices.

Reverse remodeling of left atrium assessed by cardiovascular magnetic resonance feature tracking in hypertrophic obstructive cardiomyopathy after septal myectomy.

BACKGROUND: Assessing the structure and function of left atrium (LA) is crucial in hypertrophic obstructive cardiomyopathy (HOCM) because LA remodeling correlates with atrial fibrillation. However, few studies have investigated the potential effect of myomectomy on LA phasic remodeling in HOCM after myectomy using cardiovascular magnetic resonance (CMR) feature tracking (FT). This study aims to evaluate the LA structural and functional remodeling with HOCM after myectomy by CMR-FT and to further investigate the determinants of LA reverse remodeling. METHODS: In this single-center study, we retrospectively studied 88 patients with HOCM who received CMR before and after myectomy between January 2011 and June 2021. Preoperative and postoperative LA parameters derived from CMR-FT were compared, including LA reservoir function (total ejection fraction [EF], total strain [εs], peak positive strain rate [SRs]), conduit function (passive EF, passive strain [εe], peak early negative strain rate [SRe]) and booster function (booster EF, active strain [εa], late peak negative strain rate [SRa]). Eighty-six healthy participants were collected for comparison. Univariate and multivariate linear regression identified variables associated with the rate of change of εa. RESULTS: Compared with preoperative parameters, LA reservoir function (total EF, εs, SRs), booster function (booster EF, εa, SRa), and SRe were significantly improved after myectomy (all P < 0.05), while no significant differences were observed in passive EF and εe. Postoperative patients with HOCM still had larger LA and worse LA function than healthy controls (all P < 0.05). After analyzing the rates of change in LA parameters, LA boost function, especially εa, showed the most dramatic improvement beyond the improvements in reservoir function, conduit function, and volume. In multivariable regression analysis, minimum LA volume index (adjusted ß = - 0.39, P < 0.001) and Δleft ventricular outflow tract (LVOT) pressure gradient (adjusted ß = - 0.29, P = 0.003) were significantly related to the rate of change of εa. CONCLUSIONS: Patients with HOCM after septal myectomy showed LA reverse remodeling with a reduction in LA size and restoration in LA reservoir and booster function but unchanged LA conduit function. Among volumetric and functional changes, booster function had the greatest improvement postoperatively. Besides, preoperative LAVmin index and ΔLVOT might be potential factors associated with the degree of improvement in εa.

Correlation between left ventricular fractal dimension and impaired strain assessed by cardiac MRI feature tracking in patients with left ventricular noncompaction and normal left ventricular ejection fraction.

OBJECTIVES: To investigate the correlation between the extent of excessive trabeculation assessed by fractal dimension (FD) and myocardial contractility assessed by cardiac MRI feature tracking in patients with left ventricular noncompaction (LVNC) and normal left ventricular ejection fraction (LVEF). METHODS: Forty-one LVNC patients with normal LVEF (≥ 50%) and 41 healthy controls were retrospectively included. All patients fulfilled three available diagnostic criteria on MRI. Cardiac MRI feature tracking was performed on cine images to determine left ventricular (LV) peak strains in three directions: global radial strain (GRS), global circumferential strain (GCS), and global longitudinal strain (GLS). The complexity of excessive trabeculation was quantified by fractal analysis on short-axis cine stacks. RESULTS: Compared with controls, patients with LVNC had impaired GRS, GCS, and GLS (all p < 0.05). The global, maximal, and regional FD values of the LVNC population were all significantly higher than those of the controls (all p < 0.05). Global FD was positively correlated with the end-diastolic volume index, end-systolic volume index, and stroke volume index (r = 0.483, 0.505, and 0.335, respectively, all p < 0.05), but negatively correlated with GRS and GCS (r = - 0.458 and 0.508, respectively, both p < 0.001). Moreover, apical FD was also weakly associated with LVEF and GLS (r = - 0.249 and 0.252, respectively, both p < 0.05). CONCLUSION: In patients with LVNC, LV systolic dysfunction was detected early by cardiac MRI feature tracking despite the presence of normal LVEF and was associated with excessive trabecular complexity assessed by FD. KEY POINTS: • Left ventricular global strain was already impaired in patients with extremely prominent excessive trabeculation but normal left ventricular ejection fraction. • An increased fractal dimension was associated with impaired deformation in left ventricular noncompaction.

Electrostatic Adsorption Behaviors of Charged Polymer-Tethered Nanoparticles on Oppositely Charged Surfaces.

Polymer-grafted hairy nanoparticles (HNPs) that combine the unique properties of inorganic nanoparticles (NPs) and polymers are attractive building blocks for the layer-by-layer assembly of functional hybrid materials, but the adsorption behaviors of HNPs on substrates remain unclear. This article describes a systematic study on the adsorption behaviors of charged polymer-grafted HNPs on oppositely charged substrates in different solvent media via a combination of experiments and simulations. It is shown in simulations that the adsorption process of HNPs is associated with the release of counterions around charged polymers on HNPs, thus resulting in a higher energy barrier of NP adsorption than bare NPs without charged polymer tethers. This energy barrier decreases with decreasing the dielectricity of solvents or ionization degree of grafted polymers or increasing ionic strength of the solution. Furthermore, the theoretical prediction is confirmed in experiments by using a model system of poly(acrylic acid)-grafted silica NPs and poly(diallyldimethylammonium chloride)-modified wafers. The work provides guidance for the electrostatic assembly of HNPs into functional hybrid composites with applications in membranes, optical devices, and biomedicines.

Block-Random Copolymer-Micellization-Mediated Formation of Polymeric Patches on Gold Nanoparticles.

Patchy colloidal nanoparticles are important for a broad range of applications, especially as building blocks for complex and functional structural materials, but the controllable generation of chemical patches on as-synthesized nanoparticles remains a challenge. This article describes a robust strategy for the scalable synthesis of high-quality patchy nanoparticles in high yield and solid content. A simple thermal treatment of a mixture of gold nanoparticles and thiol-terminated block-random copolymers in selected solvents produced a variety of patchy nanoparticles with a controlled morphology and number of polymeric patches (e.g., beanlike patch, one patch, two patches, three patches, multiple patches, and open-configuration patch). We show in experiments and simulations that the dynamic detachment/attachment of copolymers and the exchange of copolymers between the nanoparticle surface and free micelles in the solution-which are dictated by the architecture of copolymers-govern the formation of polymeric patches. This work not only offers an effective approach to patchy nanoparticles but also provides new insights into the phase behaviors of copolymers on nanoscale surfaces.

Transcriptome-wide identification and characterization of toll-like receptors response to Vibrio anguillarum infection in Manila clam (Ruditapes philippinarum).

The Manila clam (Ruditapes philippinarum), one of the major marine aquaculture species in China, is susceptible to infection with the pathogen Vibrio, which results in massive mortality and economic losses. Toll-like receptors (TLRs) are significant pattern recognition receptors (PRRs) of innate immunity that are involved in immune regulation against pathogenic invasion. Molecular characterization of Manila clam TLRs and investigations of their immune functions are essential to prevent and control Vibrio infection. In the present research, eight cDNA sequences of R. philippinarum TLRs (RpTLRs) were identified from previous transcriptome libraries and then classified into four groups, namely, P-TLR (one sequence), V-TLR (one sequence), Ls-TLR (two sequences) and sP-TLR (four sequences), based on the corresponding LRR domain arrangement of their protein structures within the typical TLR motifs. A selective pressure test firstly suggested that the molluscan P-TLR, V-TLR, Ls-TLR and sP-TLR families underwent positive selection, and different numbers of positive selection sites (PSSs) were identified in different domains of the four types of RpTLRs, as determined by PAML and analysis of website data. These findings indicated that the evolution of RpTLRs may be associated with their immune recognition and function. Furthermore, tissue-specific expression analysis showed that all RpTLRs were ubiquitously expressed in all test tissues and were dominant in hemocytes. Quantitative real-time PCR revealed that the cDNA expression of all eight RpTLRs was upregulated after injection with Vibrio anguillarum (P < 0.01) in R. philippinarum hemocytes, revealing that these RpTLRs play important roles in responding to pathogenic stimulation. In summary, these findings provide a foundation for future investigations of the molecular classification and evolutionary patterns of Toll-like receptors in invertebrates, and the innate immune responses of TLR signaling pathways in Mollusca.

Application of functionalized graphene in Li-O2 batteries.

Li-O2 batteries (LOB) are considered as one of the most promising energy storage devices using renewable electricity to power electric vehicles because of its exceptionally high energy density. Carbon materials have been widely employed in LOB for its light weight and facile availability. In particular, graphene is a suitable candidate due to its unique two-dimensional structure, high conductivities, large specific surface areas, and good stability at high charge potential. However, the intrinsic catalytic activity of graphene is insufficient for the sluggish kinetics of oxygen reduction reaction (ORR) and oxygen evolution reaction (OER) in LOB. Therefore, various surface functionalization schemes for graphene have been developed to tailor the surface chemistry of graphene. In this review, the properties and performances of functionalized graphene cathodes are discussed from theoretical and experimental aspects, including heteroatomic doping, oxygen functional group modifications, and catalyst decoration. Heteroatomic doping breaks electric neutrality of sp2 carbon of graphene, which forms electron-deficient or electron-rich sites. Oxygen functional groups mainly create defective edges on graphene oxides with C-O, C=O, and -COO-. Catalyst decoration is widely attempted by various transition and precious metal and metal oxides. These induced reactive sites usually improve the ORR and/or OER in LOB by manipulating the adsorption energies of O2, LiO2, Li2O2, and promoting electron transportation of cathode. In addition, functionalized graphene is used in anode and separators to prevent shuttle effect of redox mediators and suppress growth of Li dendrite.

Detection of Performance of Hybrid Rice Pot-Tray Sowing Utilizing Machine Vision and Machine Learning Approach.

Monitoring the performance of hybrid rice seeding is very important for the seedling production line to adjust the sowing amount of the seeding device. The objective of this paper was to develop a system for the real-time online monitoring of the performance of hybrid rice seeding based on embedded machine vision and machine learning technology. The embedded detection system captured images of pot trays that passed under the illuminant cabinet installed in the seedling production line. This paper proposed an algorithm for fixed threshold segmentation by analyzing the images with the exploratory analysis method. With the algorithm, the grid image and seed image were extracted from the pot tray image. The paper also proposed a method for obtaining pixel coordinates of gridlines from the grid image. Binary images of seeds were divided into small pieces, according to the pixel coordinates of gridlines. Each piece corresponded to a cell on the pot tray. By scanning the contours in each piece of the image to check whether there were seeds in the cell, the number of empty cells was counted and then used to calculate the missing rate of hybrid rice seeding. The seed number sowed in pot trays was monitored while using the machine learning approach. The experimental results demonstrated that it would consume 4.863 s for the device to process an image, which allowed for the detection of the missing rate and seed number in real-time at the rate of 500 trays per hour (7.2 s per tray). The average accuracy of the detection of missing rates of a seedling production line was 94.67%. The average accuracy of the detection of seed numbers was 95.68%.

Preparation of Nano Au and Pt Alloy Microspheres Decorated with Reduced Graphene Oxide for Nonenzymatic Hydrogen Peroxide Sensing.

The flourish of nanotechnology has brought new vitality to the research and development of electrochemical sensing materials. In this work, we successfully synthesized Nano Au and Pt alloy microspheres decorated with reduced graphene oxide (RGO/nAPAMSs) by a simple, facile, and eco-friendly one-step reduction strategy for the fabrication of highly sensitive nonenzymatic H2O2 sensing interfaces. Energy-dispersive X-ray spectroscopy mapping (EDX mapping), energy-dispersive X-ray spectroscopy analysis (EDX), transmission electron microscopy (TEM), Fourier transform infrared spectrum (FT-IR), and X-ray diffraction spectrum (XRD) were employed to characterize RGO/nAPAMSs from a microscopic perspective. The results of cyclic voltammetry and chronoamperometry exhibited excellent electrochemical behaviors toward H2O2, with a rapid response time within 5 s, remarkable sensitivity of 1117.0 µA mM-1 cm-2, wide linear range of 0.005 to 4.0 mM and lower detection limit of 0.008 µM (S/N = 3), which provide RGO/nAPAMS not only a promising prospect for the quantitative detection of H2O2 but also a potential application in other fields of sensors. Moreover, further analysis showed the principles of the superior H2O2 sensing performance of RGO/nAPAMSs. This discovery provides a significant contribution to future study in nonenzymatic H2O2 sensing based on Nano Pt, Nano Au noble metal electrocatalysts.

PGE2 Modulates GABAA Receptors via an EP1 Receptor-Mediated Signaling Pathway.

AIMS: PGE2 is one of the most abundant prostanoids in mammalian tissues, but its effect on neuronal receptors has not been well investigated. This study examines the effect of PGE2 on GABAA receptor currents in rat cerebellar granule neurons. METHODS: GABAA currents were recorded using a patch-clamp technique. Cell surface and total protein of GABAA ß1/2/3 subunits was carried out by Western blot analysis. RESULTS: Upon incubation of neurons with PGE2 (1 µM) for 60 minutes, GABAA currents were significantly potentiated. This PGE2-driven effect could be blocked by PKC or CaMKII inhibitors as well as EP1 receptor antagonist, and mimicked by PMA or EP1 receptor agonist. Furthermore, Western blot data showed that PGE2 did not increase the total expression level of GABAA receptors, but significantly increased surface levels of GABAA ß1/2/3 subunits after 1 h of treatment. Consistently, both PKC and CaMKII inhibitors were able to reduce PGE2-induced increases in cell surface expression of GABAA receptors. CONCLUSION: Activation of either the PKC or CaMKII pathways by EP1 receptors mediates the PGE2-induced increase in GABAA currents. This suggests that upregulation of postsynaptic GABAA receptors by PGE2 may have profound effects on cerebellar functioning under physiological and pathological conditions.

Daily Estimation of Ground-Level PM2.5 Concentrations over Beijing Using 3 km Resolution MODIS AOD.

Estimating exposures to PM2.5 within urban areas requires surface PM2.5 concentrations at high temporal and spatial resolutions. We developed a mixed effects model to derive daily estimations of surface PM2.5 levels in Beijing, using the 3 km resolution satellite aerosol optical depth (AOD) calibrated daily by the newly available high-density surface measurements. The mixed effects model accounts for daily variations of AOD-PM2.5 relationships and shows good performance in model predictions (R(2) of 0.81-0.83) and cross-validations (R(2) of 0.75-0.79). Satellite derived population-weighted mean PM2.5 for Beijing was 51.2 µg/m(3) over the study period (Mar 2013 to Apr 2014), 46% higher than China's annual-mean PM2.5 standard of 35 µg/m(3). We estimated that more than 19.2 million people (98% of Beijing's population) are exposed to harmful level of long-term PM2.5 pollution. During 25% of the days with model data, the population-weighted mean PM2.5 exceeded China's daily PM2.5 standard of 75 µg/m(3). Predicted high-resolution daily PM2.5 maps are useful to identify pollution "hot spots" and estimate short- and long-term exposure. We further demonstrated that a good calibration of the satellite data requires a relatively large number of ground-level PM2.5 monitoring sites and more are still needed in Beijing.

Recent wetting trend over Taklamakan and Gobi Desert dominated by internal variability.

The Taklamakan and Gobi Desert (TGD) region has experienced a pronounced increase in summer precipitation, including high-impact extreme events, over recent decades. Despite identifying large-scale circulation changes as a key driver of the wetting trend, understanding the relative contributions of internal variability and external forcings remains limited. Here, we approach this problem by using a hierarchy of numerical simulations, complemented by diverse statistical analysis tools. Our results offer strong evidence that the atmospheric internal variations primarily drive this observed trend. Specifically, recent changes in the North Atlantic Oscillation have redirected the storm track, leading to increased extratropical storms entering TGD and subsequently more precipitation. A clustering analysis further demonstrates that these linkages predominantly operate at the synoptic scale, with larger contributions from large precipitation events. Our analysis highlights the crucial role of internal variability, in addition to anthropogenic forcing, when seeking a comprehensive understanding of future precipitation trends in TGD.

Atmospheric rivers over eastern US affected by Pacific/North America pattern.

Atmospheric rivers (ARs) play important roles in various extreme weather events across the US. While AR features in western US have been extensively studied, there remains limited understanding of their variability in the eastern US (EUS). Using both observations and a state-of-the-art climate model, we find a significant increase (~10% dec-1) in winter AR frequency in the EUS during the past four decades. This trend is closely linked to recent changes in the Pacific/North America (PNA) teleconnection pattern, accompanied by a poleward shift of the mid-latitude jet stream. We further reveal a strong correlation (R = 0.8; P < 0.001) between interannual variations in AR occurrence and the PNA index. This linkage has been verified in various model simulations. A statistical model, built on this linkage, has proven effective in predicting the AR frequency using the PNA index at both monthly and seasonal scales. These promising results have important implications for addressing concerns related to AR-associated extreme precipitation and flooding in this region.

The ncRNA-mediated regulatory networks of defensins and lysozymes in Riptortus pedestris: involvement in response to gut bacterial disturbances.

Insects depend on humoral immunity against intruders through the secretion of antimicrobial peptides (AMPs) and immune effectors via NF-κB transcription factors, and their fitness is improved by gut bacterial microbiota. Although there are growing numbers of reports on noncoding RNAs (ncRNAs) involving in immune responses against pathogens, comprehensive studies of ncRNA-AMP regulatory networks in Riptortus pedestris, which is one of the widely distributed pests in East Asia, are still not well understood under feeding environmental changes. The objective of this study employed the whole-transcriptome sequencing (WTS) to systematically identify the lncRNAs (long noncoding RNA) and circRNAs (circular RNA) and to obtain their differential expression from the R. pedestris gut under different feeding conditions. Functional annotation indicated that they were mainly enriched in various biological processes with the GO and KEGG databases, especially in immune signaling pathways. Five defensin (four novel members) and eleven lysozyme (nine novel members) family genes were identified and characterized from WTS data, and meanwhile, phylogenetic analysis confirmed their classification. Subsequently, the miRNA-mRNA interaction network of above two AMPs and lncRNA-involved ceRNA (competing endogenous RNA) regulatory network of one lysozyme were predicted and built based on bioinformatic prediction and calculation, and the expression patterns of differentially expressed (DE) defensins, and DE lysozymes and related DE ncRNAs were estimated and selected among all the comparison groups. Finally, to integrate the analyses of WTS and previous 16S rRNA amplicon sequencing, we conducted the Pearson correlation analysis to reveal the significantly positive or negative correlation between above DE AMPs and ncRNAs, as well as most changes in the gut bacterial microbiota at the genus level of R. pedestris. Taken together, the present observations provide great insights into the ncRNA regulatory networks of AMPs in response to rearing environmental changes in insects and uncover new potential strategies for pest control in the future.

Case report: Safety and efficacy of synergistic treatment using selinexor and azacitidine in patients with atypical chronic myeloid leukemia with resistance to decitabine.

Background: Atypical chronic myeloid leukemia (aCML) is a BCR::ABL1 negative myelodysplastic/myeloproliferative neoplasm with poor overall survival. Some patients can be treated by allogeneic hematopoietic stem cell transplantation (allo-HSCT) from suitable donors. The effectiveness of decitabine or azacitidine (AZA) has recently been reported; however, their combined efficacy with selinexor has not yet been reported. Case description: In this study, we report the case of a patient with aCML who was successfully treated with selinexor combined with AZA. A 67-year-old man with a history of gastric mucosa-associated lymphoid tissue (MALT) lymphoma was admitted to the hospital with fatigue and emaciation. He was diagnosed with aCML and no longer responded to decitabine treatment after undergoing seven cycles. The patient was subsequently administered hydroxyurea (HU), selinexor, and AZA. After four courses of combination therapy, his blood cell counts improved; he no longer required transfusions and was able to discontinue HU. The patient continued receiving selinexor and AZA without severe complications. This case is the first to show that combinatorial selinexor and AZA therapy can effectively treat aCML. Conclusion: Our case sheds light on the importance of selinexor and AZA combined therapy in the exploration of new treatment strategies for aCML. Moreover, this treatment approach offers the possibility of bridging with allo-HSCT.

Development of NaCl-MgCl2-CaCl2 Ternary Salt for High-Temperature Thermal Energy Storage Using Machine Learning.

NaCl-MgCl2-CaCl2 eutectic ternary chloride salts are potential heat transfer and storage materials for high-temperature thermal energy storage. In this study, first-principles molecular dynamics simulation results were used as a data set to develop an interatomic potential for ternary chloride salts using a neural network machine learning method. Deep potential molecular dynamics (DPMD) simulations were performed to predict the microstructure and thermophysical properties of the NaCl-MgCl2-CaCl2 ternary salt. This work reveals that DPMD simulations can accurately calculate the microstructure and thermophysical properties of ternary chloride salts. The association strength of chloride ions and cations follows the order of Mg2+ > Ca2+ > Na+, and the coordination number decreases gradually with increasing temperature, indicating a progressively looser and more disordered molten structure. Furthermore, thermophysical properties, such as density, specific heat capacity, thermal conductivity, and viscosity, are in good agreement with the experimental measurements. Machine learning molecular dynamics will provide a feasible multivariate molten salt exploration method for the design of next-generation solar power plants and thermal energy storage systems.