RESUMO
Development of an effective, broadly-active and safe vaccine for protection of poultry from H5N1 highly pathogenic avian influenza viruses (HPAIVs) remains an important practical goal. In this study we used a low pathogenic wild aquatic bird virus isolate Ð/duck/Moscow/4182/2010 (H5N3) (dk/4182) as a live candidate vaccine. We compared this virus with four live 1:7 reassortant anti-H5N1 candidate vaccine viruses with modified hemagglutinin from either A/Vietnam/1203/04 (H5N1) or A/Kurgan/3/05 (H5N1) and the rest of the genes from either H2N2 cold-adapted master strain A/Leningrad/134/17/57 (rVN-Len and rKu-Len) or H6N2 virus A/gull/Moscow/3100/2006 (rVN-gull and rKu-gull). The viruses were tested in parallel for pathogenicity, immunogenicity and protective effectiveness in chickens using aerosol, intranasal and oral routes of immunization. All five viruses showed zero pathogenicity indexes in chickens. Viruses rVN-gull and rKu-gull were immunogenic and protective, but they were insufficiently attenuated and caused significant mortality of 1-day-old chickens. The viruses with cold-adapted backbones (rVN-Len and rKu-Len) were completely nonpathogenic, but they were significantly less immunogenic and provided lower protection against lethal challenge with HPAIV A/Chicken/Kurgan/3/05 (H5N1) as compared with three other vaccine candidates. Unlike other four viruses, dk/4182 was both safe and highly immunogenic in chickens of any age regardless of inoculation route. Single administration of 106 TCID50 of dk/4182 virus via drinking water provided complete protection of 30-days-old chickens from 100 LD50 of the challenge virus. Our results suggest that low pathogenic viruses of wild aquatic birds can be used as safe and effective live poultry vaccines against highly pathogenic avian viruses.
Assuntos
Galinhas , Imunização , Virus da Influenza A Subtipo H5N1/imunologia , Vacinas contra Influenza/imunologia , Influenza Aviária/prevenção & controle , Doenças das Aves Domésticas/prevenção & controle , Administração Oral , Envelhecimento , Animais , Relação Dose-Resposta Imunológica , Ensaio de Imunoadsorção Enzimática , Genoma Viral , Virus da Influenza A Subtipo H5N1/patogenicidade , Vacinas contra Influenza/administração & dosagem , Doenças das Aves Domésticas/virologia , VirulênciaRESUMO
Four H5N2 experimental vaccine strains and the apathogenic wild duck H5N3 influenza virus A/duck/ Moscow/4182/2010 (dk/4182) were tested as a live poultry vaccine. Experimental strains had the hemagglutinin of the A/Vietnam/1203/04 strain lacking the polybasic HA cleavage site or the hemagglutinin from attenuated virus (Ku/ at) that was derived from the highly pathogenic influenza virus A/chicken/Kurgan/3/2005 (H5N1). The hemagglutinin of the Ku-at has the amino acid substitutions Asp54/Asn and Lys222/Thr in HA1 and Val48/Ile and Lys131/Thr in HA2, while maintaining the polybasic HA cleavage site at an invariable level. The other genes of these experimental strains were from the H2N2 cold-adapted master strain A/Leningrad/134/17/57 (VN-Len and Ku-Len) or from the apathogenic H6N2 virus A/gull/Moscow/3100/2006 (VN-Gull and Ku-Gull). A single immunization of mice with all tested strains elicited a high level of serum antibodies and provided complete protection against the challenge with the lethal dose of A/chicken/Kurgan/3/05. The pathogenicity indexes of the Ku-at and the other strains for chicken were virtually zero, whereas the index of the parent H5N1 virus A/chicken/Kurgan/3/2005 was 2.98. Intravenous, intranasal, and aerosol routes of vaccination were compared. It was shown that the strain dk/4182 was totally apathogenic for one-day-old chicken and provided complete protection against the highly pathogenic H5N1 virus.
Assuntos
Galinhas , Vírus da Influenza A/imunologia , Vacinas contra Influenza/imunologia , Influenza Aviária , Doenças das Aves Domésticas , Animais , Galinhas/imunologia , Galinhas/virologia , Influenza Aviária/imunologia , Influenza Aviária/prevenção & controle , Camundongos , Doenças das Aves Domésticas/imunologia , Doenças das Aves Domésticas/prevenção & controle , Vacinas Atenuadas/imunologiaRESUMO
Monitoring programs for highly dangerous avian diseases in the Russian Federation from 2001 to 2009 detected 77 samples that were PCR positive for avian paramyxovirus serotype-1 (APMV-1) from sick or dead feral and domestic pigeons. Nucleotide sequences of the fusion (F) gene, including a nucleotide sequence encoding the F protein cleavage site, were determined for these isolates. All of the studied isolates possessed virulent F0 protein cleavage sites (112KRKKRF117, 112RRQKRF117, or 112KRQKRF117). Intracerebral pathogenicity index (ICPI) values determined for seven of the isolates exceeded the value of 0.7 (the range from 0.8 to 1.41). Based on partial genome sequencing and phylogenetic analysis, the isolates were assigned to two individual sublineages within class II genotype VIb. It was determined that most of these Newcastle disease virus isolates (70/77) recovered from the pigeons belonged to a relatively poorly studied sublineage VIb/2. The complete nucleotide sequence of the genome for the Pigeon/Russia/Vladimir/687/05 isolate of sublineage VIb/2 was determined.
Assuntos
Columbidae , Genes Virais , Doença de Newcastle/virologia , Vírus da Doença de Newcastle/genética , Vírus da Doença de Newcastle/patogenicidade , Animais , Dados de Sequência Molecular , Vírus da Doença de Newcastle/classificação , Vírus da Doença de Newcastle/isolamento & purificação , Filogenia , Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária , Federação Russa , Análise de Sequência de Proteína/veterinária , Análise de Sequência de RNA/veterinária , Homologia de Sequência , VirulênciaRESUMO
The experimental reassortant vaccine strain VN-gull (H5N2) containing H5 hemagglutinin (HA) with a removed polybasic site in the connecting peptide and other genes from the apathogenic H6N2 virus A/gull/Moscow/3100/2006 (gull/M) was obtained using a two-step protocol. At Step 1, the reassortant with HA of A/Vietnam/1203/04-PR8/ CDC-RG and other genes from cold-adapted A/Leningrad/17/47 (VN-Len) viruses was generated due to selection with antibody to H2N2 at 26 degrees C. At Step 2, the reassortant VN-gull was obtained by replacing all genes from Len with those from gull/M due to selection with antibody to H6N2 at 39 degrees C. The reassortant VN-Len was apathogenic and the reassortant VN-gull was weakly virulent in mice. Both gave rise to specific antibodies and 4 weeks after single inoculation they provided complete protection against further challenge with highly pathogenic HSN1 virus A/chicken/Kurgan/3/05 (H5N1) (Ku-Len). The chickens infected with live VN-gull virus showed neither clinical symptoms, nor fecal virus excretion; nevertheless, they gave rise to antibodies and were protected from the further challenge with A/chicken/Kurgan/3/2005. The high yield, safety, and protectivity of VN-Len and Ku-Len made them promising strains for the production of inactivated and live vaccines against H5N1 viruses.
Assuntos
Vírus da Influenza A Subtipo H5N2 , Vacinas contra Influenza , Vírus Reordenados , Temperatura , Vacinas Atenuadas , Animais , Anticorpos Antivirais/biossíntese , Anticorpos Antivirais/imunologia , Charadriiformes/imunologia , Embrião de Galinha , Galinhas/imunologia , Glicoproteínas de Hemaglutininação de Vírus da Influenza/química , Humanos , Vírus da Influenza A Subtipo H5N2/genética , Vírus da Influenza A Subtipo H5N2/imunologia , Vacinas contra Influenza/genética , Vacinas contra Influenza/imunologia , Influenza Aviária/imunologia , Influenza Aviária/prevenção & controle , Influenza Humana/imunologia , Influenza Humana/prevenção & controle , Camundongos , Modelos Animais , Vírus Reordenados/genética , Vírus Reordenados/imunologia , Vacinação , Vacinas Atenuadas/genética , Vacinas Atenuadas/imunologia , Replicação ViralRESUMO
In this study, we report the development and validation of a duplex real-time polymerase chain reaction (PCR) assay with an internal control using TaqMan-labelled probes for the detection of Mycoplasma gallisepticum and Mycoplasma synoviae (duplex MGMS PCR). The MGMS PCR was highly specific with a sensitivity of 7 and 1 colony-forming units/ml for M. gallisepticum and M. synoviae, respectively, using dilution of pure culture that corresponds to 34 and 29 DNA copies per reaction. Validation of the assay was completed with 260 and 27 pooled samples (tracheal swabs) from commercial chickens and turkeys, respectively, with potential M. gallisepticum and M. synoviae involvement and 42 samples (palatine cleft swabs) from backyard geese and ducks. Using isolation as the gold standard, the MGMS PCR was more sensitive than isolation and the analytical sensitivity was 0.944 and 0.958 for M. gallisepticum and M. synoviae, respectively. In comparison with a gapA-based assay (gapA PCR) and a 16S rRNA-based assay (16S PCR) for M. gallisepticum and M. synoviae, respectively, the results agreed for 94.5% and 96.6%, respectively. The use of the internal control allowed monitoring of proper extraction and inhibition of amplification that was detected in 12 samples. The duplex MGMS PCR was shown to be superior to the presently reported real-time PCR assays in terms of combination of sensitivity, specificity and capacity of detection of more than one target in a single tube. In conclusion, the duplex MGMS PCR was highly specific, sensitive, and reproducible and could be used on clinical samples from commercial chickens, turkeys and backyard poultry including ducks and geese.
Assuntos
Infecções por Mycoplasma/veterinária , Mycoplasma gallisepticum/isolamento & purificação , Mycoplasma synoviae/isolamento & purificação , Reação em Cadeia da Polimerase/métodos , Doenças das Aves Domésticas/microbiologia , Animais , Galinhas , Contagem de Colônia Microbiana , DNA Bacteriano/genética , Técnicas de Sonda Molecular , Infecções por Mycoplasma/microbiologia , Mycoplasma gallisepticum/genética , Mycoplasma synoviae/genética , RNA Bacteriano/genética , Reprodutibilidade dos Testes , PerusRESUMO
The genetic diversity of the pvpA gene of Mycoplasma gallisepticum (MG) samples originating from commercial chickens was investigated. In the present study, we evaluated the genetic variability of 26 field samples of MG detected in commercial chickens and turkeys from 18 regions of Russia and compared them to the reference strains of MG available in GenBank. Genetic variability was evaluated by partial nucleotide sequencing of the pvpA gene, which encodes a putative cytadhesin protein. Comparisons with MG strains and isolates from the United States, Australia, China, and Iran using sequence analysis of PCR products showed that Russian MG field samples clustered more closely to each other than to the international reference MG strains. The MG pvpA sequences were found to be highly variable with a discrimination index of 0.975 for Russian field samples. No apparent cluster was found using the criteria of year or location of detection. DNA sequence polymorphism and size variation in the pvpA gene were shown among the Russian MG field samples and could be used for MG typing. These findings might help better understand the relationship among MG isolates from Russia and other countries.
Assuntos
Adesinas Bacterianas/genética , Variação Genética , Infecções por Mycoplasma/veterinária , Mycoplasma gallisepticum/genética , Doenças das Aves Domésticas/microbiologia , Animais , Sequência de Bases , Galinhas , Dados de Sequência Molecular , Infecções por Mycoplasma/epidemiologia , Infecções por Mycoplasma/microbiologia , Mycoplasma gallisepticum/isolamento & purificação , Filogenia , Doenças das Aves Domésticas/epidemiologia , Federação Russa/epidemiologia , PerusRESUMO
The paper presents data on the comparative analysis of nucleotide sequences of a S3 gene fragment of 67 chicken reovirus (CRV) isolates from the abnormal biopsy specimens tested in 1999 to 2007. These CRV isolates were ascertained to differ from vaccine strains in the nucleotide sequence of the S3 gene. The approximate substitution rates for the S3 gene were established to range from 2.0 x 10(-3) to 6.0 x 10(-3) nucleotide substitutions per year.
Assuntos
Proteínas do Capsídeo/genética , Galinhas/genética , Doenças das Aves Domésticas/virologia , Proteínas de Ligação a RNA/genética , Infecções por Reoviridae/veterinária , Reoviridae/genética , Animais , Sequência de Bases , Proteínas do Capsídeo/classificação , Dados de Sequência Molecular , Filogenia , Proteínas de Ligação a RNA/classificação , Reoviridae/isolamento & purificação , Infecções por Reoviridae/virologia , Federação Russa , Análise de Sequência de DNA , Homologia de Sequência do Ácido NucleicoRESUMO
Isolation, followed by the sequencing the full-size genome of strains of A/chicken/Krasnodarl300/07 and A/Cygnus cygnus/Krasnodar/329/07, has shown that they belong to genotype 2.2 (Qinghai-Siberian). The strains were deposited at the State Virus Collection of the Russian Federation and nucleotide consequences were at the International databank GenBank. The strains contained 10 unique amino acid replacements in reference to the consensus of the Qinghai-Siberian genotype in the PB2, PA, HA, NA, and NS1, which suggests that regional variants may form in different parts of an area.
Assuntos
Animais Selvagens/virologia , Aves/virologia , Surtos de Doenças/veterinária , Virus da Influenza A Subtipo H5N1/classificação , Influenza Aviária/epidemiologia , Influenza Aviária/virologia , Substituição de Aminoácidos , Animais , Linhagem Celular , Embrião de Galinha , Cães , Variação Genética , Genoma Viral , Virus da Influenza A Subtipo H5N1/genética , Virus da Influenza A Subtipo H5N1/isolamento & purificação , Dados de Sequência Molecular , Filogenia , Aves Domésticas/virologia , Federação Russa/epidemiologia , Suínos , Proteínas Virais/genética , ZoonosesRESUMO
The recombinant antigen obtained by cloning and expressing two IBV nucleocapsid protein fragments (143-414 aa, 281-414 aa) in Escherichia coli was used for the detection of avian infectious bronchitis virus (IBV) specific antibodies in chicken sera by the indirect ELISA (rNpIBV-ELISA). As a result of testing 1524 serum samples the diagnostic sensitivity and specificity of rNpIBV-ELISA when comparing those of the routine whole IBV ELISA have been shown to be 93.81% and 87.36%, respectively. The agreement value was 91.5%.
Assuntos
Anticorpos Antivirais/sangue , Ensaio de Imunoadsorção Enzimática/métodos , Vírus da Bronquite Infecciosa/imunologia , Proteínas do Nucleocapsídeo/imunologia , Animais , Embrião de Galinha , Galinhas , Proteínas Recombinantes/imunologia , VacinaçãoRESUMO
The method of chicken reovirus strain differentiation was worked out on the basis of RT-PCR and heteroduplex mobility assay (HMA). The S3 gene cDNA (633-896 b.p.) of some Russian and Italian chicken reovirus isolates was amplified by RT-PCR. The analysis of these cDNA samples was carried out by HMA. The relation between nucleotide differences and relative mobility of compared cDNA heteroduplex was reflected by the regression curve. The equation of linear regression was derived (y = 91.726-0.89x; where y is the level of nucleotide difference of compared cDNA (%), x is the relative mobility of compared cDNA heteroduplex (%)). This method made it possible to take correct results within 5-35% of nucleotide difference in heteroduplex sequences.
Assuntos
Doenças das Aves Domésticas/virologia , Infecções por Reoviridae/veterinária , Reoviridae/classificação , Animais , Proteínas do Capsídeo/genética , Galinhas/virologia , DNA Complementar/análise , Análise Heteroduplex , Proteínas de Ligação a RNA/genética , Análise de Regressão , Reoviridae/genética , Reoviridae/isolamento & purificação , Infecções por Reoviridae/virologia , Reação em Cadeia da Polimerase Via Transcriptase ReversaRESUMO
A field chick infectious bronchitis virus (IBV) was isolated from the pathological material on chick embryos. The nucleotide sequence of the S1 gene was determined and comparatively analyzed with some sequences of this gene of foreign and Russian vaccine strains and isolates. A cross-neutralization test using sera to various IBV seroptypes was performed. The isolate was shown to antigenically differ from the reference strains. Bioassay was carried out, by using one-day chicks and the immunogenic properties of the virus were investigated.
Assuntos
Infecções por Coronavirus/veterinária , Surtos de Doenças , Vírus da Bronquite Infecciosa/classificação , Doenças das Aves Domésticas/epidemiologia , Doenças das Aves Domésticas/virologia , Animais , Anticorpos Antivirais/imunologia , Células Cultivadas , Embrião de Galinha , Galinhas/virologia , Infecções por Coronavirus/epidemiologia , Infecções por Coronavirus/virologia , Genes Virais/genética , Hemaglutininas Virais , Vírus da Bronquite Infecciosa/genética , Vírus da Bronquite Infecciosa/imunologia , Vírus da Bronquite Infecciosa/isolamento & purificação , Vírus da Bronquite Infecciosa/patogenicidade , Glicoproteínas de Membrana/genética , Dados de Sequência Molecular , Testes de Neutralização , Federação Russa/epidemiologia , Análise de Sequência de Proteína , Especificidade da Espécie , Glicoproteína da Espícula de Coronavírus , Proteínas do Envelope Viral/genética , VirulênciaRESUMO
RNAs from Escherichia coli cells, Syrian hamster kidney cells, foot-and-mouth disease virus, and Newcastle disease virus were isolated using glass fiber filters GF/F or GF/C. The RNA was reversibly adsorbed on the filters in the presence of 2 M guanidine thiocyanate and 50% ethanol (or isopropanol) and eluted with water. The fraction composition of the isolated RNA depended on the guanidine thiocyanate and alcohol concentrations in the adsorption and washing procedures. The RNA preparations obtained by this method can be used in reverse transcription and reverse transcription-polymerase chain reaction without additional purification.
Assuntos
RNA Bacteriano/isolamento & purificação , RNA Viral/isolamento & purificação , RNA/isolamento & purificação , Animais , Cricetinae , Escherichia coli/genética , Filtração/métodos , Guanidinas , Rim/química , Mesocricetus , Picornaviridae/genética , Respirovirus/genética , TiocianatosRESUMO
Variable cDNA regions in the VP2 gene of 24 isolates of infectious bursal disease virus (IBDV) isolated in Russia in 1993-1996 were amplified by the "nested" PCR and sequenced. The primary structure analysis of the VP2 gene variable region revealed 2 major groups of IBDV isolates. The first group consisted of the isolates with the structure identical or closely related to the highly virulent European strains CS89, 74/89A, 661, JY86, and DV86, the second group included the isolates with a high level of homology to the vaccine strains PBG98 and Cu-1. In addition, two isolates with original structure were identified, which differed from previously studied strains.
Assuntos
Vírus da Doença Infecciosa da Bursa/genética , Proteínas Estruturais Virais/genética , Sequência de Aminoácidos , Sequência de Bases , DNA Viral , Vírus da Doença Infecciosa da Bursa/imunologia , Dados de Sequência Molecular , Homologia de Sequência de Aminoácidos , Vacinas Virais/genéticaRESUMO
Synthesis, cDNA cloning, and nucleotide sequencing of F gene of rinderpest virus strain K was carried out. Analysis of nucleotide sequence showed the only open reading frame coding for protein from 546 a.o. with mol. weight 58.6 kDa. The mean percentage of identical nucleotide residues between F genes of strains K, Kabete O, and L is 76.4% for 5'-untranslated region and 90.5% for translated region, the share of similar amino acid residues in the respective proteins is 92.9%. The structure of restriction site of F0 precursor protein in rinderpest strains with different virulence is similar. Protein F of rinderpest virus strain K has 3 potential glycosylation sites and 13 cystein residues in positions identical to those of F protein of rinderpest strains Kabete O and L.
Assuntos
Genes Virais , Glicoproteínas/genética , Vírus da Peste Bovina/genética , Proteínas Virais de Fusão/genética , Regiões 5' não Traduzidas , Sequência de Aminoácidos , Animais , Sequência de Bases , Bovinos , Clonagem Molecular , DNA Complementar , Glicoproteínas/química , Proteínas de Membrana , Dados de Sequência Molecular , Fases de Leitura Aberta , Homologia de Sequência de Aminoácidos , Homologia de Sequência do Ácido Nucleico , Proteínas Virais de Fusão/químicaRESUMO
The nucleotide sequence of a part of the HindIII-D fragment (3300 b.p.) of adenovirus KR95 DNA has been determined. Analysis of the nucleotide sequence disclosed a continuous ORF for hexon gene (2814 b.p.) coding the 937 residue protein, part of ORF for the C-terminal region of pVI polypeptide, including 114 residues and the beginning of ORF coding 25 N-terminal residues for viral endoproteinase. Comparison of predicted KR95 hexon sequence and 8 mammalian and avian adenovirus hexon sequences revealed the highest homology between KR95 strain and avian adenoviruses FAV10 and FAV1 (91.1 and 80.1%, respectively). The results were used for creating a test system on the basis of the polymerase chain reaction. The system was used in analysis of fowl samples obtained from 12 poultry farms in Russia. The sequences of hexon gene amplified fragments in the isolated strains and similar fragments of other mammalian and avian adenoviruses have been compared.
Assuntos
Adenoviridae/genética , Antígenos Virais/genética , Proteínas do Capsídeo , Capsídeo/genética , Galinhas/virologia , Derrame Pericárdico/veterinária , Doenças das Aves Domésticas/genética , Adenoviridae/isolamento & purificação , Sequência de Aminoácidos , Animais , Sequência de Bases , Dados de Sequência Molecular , Derrame Pericárdico/genética , Derrame Pericárdico/virologia , Doenças das Aves Domésticas/virologia , Homologia de Sequência de Aminoácidos , Homologia de Sequência do Ácido Nucleico , SíndromeRESUMO
The cDNA fragments complementary to RNA-polymerase gene and 3'-untranslated genome region of attenuated foot-and-mouth disease virus strain A(22)645 have been synthesized and cloned into a plasmid vector pUC19 in E. coli JM109. The cloned cDNA fragments were characterized as to their size, orientation towards the plasmid, and localization in the virus genome. Restriction maps for complete gene and two cDNA clones were constructed.
Assuntos
Aphthovirus/genética , RNA Polimerases Dirigidas por DNA/genética , Fragmentos de Peptídeos/genética , Aphthovirus/enzimologia , Aphthovirus/imunologia , Clonagem Molecular , DNA Complementar , RNA Polimerases Dirigidas por DNA/química , Escherichia coli/genética , Plasmídeos , Vacinas Atenuadas/genética , Vacinas Virais/genéticaRESUMO
Synthesis, cDNA cloning, and identification of H gene nucleotide sequence of rinderpest virus (RPV) K strain are carried out. Analysis of the identified nucleotide sequence has revealed the single open reading frame encoding a protein consisting of 609 amino acids with molecular weight of 68 kDa. The mean nucleotide homology between H genes of K, Kabete O and L strains in 88.0%, the mean amino acid homology of the corresponding proteins is 88.2%. RPV K strain hemagglutinin contains 5 potential glycosylation sites. The position of all 13 cystein bases is identical to positions in H proteins of RPV Kabete O and L strains. Studies of the hydrophobic profile of the compared proteins have shown 2 potential transmembrane fragments.
Assuntos
DNA Viral/genética , Vírus da Peste Bovina/genética , Animais , Sequência de Bases , Bovinos , Clonagem Molecular , DNA Complementar , Genes Virais , Dados de Sequência Molecular , Proteínas Estruturais Virais/genéticaRESUMO
Primary structure of capsid proteins and RNA polymerase of three closely related strains of foot and mouth disease virus (FMDV), subtype A22, differing by biological properties (the initial epitheliotropic strain A22 550 and its derivatives: thermoresistant myotropic A22 550/4 and thermosensitive attenuated A22 645) are compared by nucleic acid sequencing and analysis of the amino acid sequencing. The study revealed 1 substitute in VPI and 8 in RNA polymerase in the myotropic variant and 1 substitute in VP2, 2 in VP3, 13 in VP1, and 3 in RNA polymerase. Alteration of A22 550/4 tropism is probably due to a single substitution Gly 145-->Thr in the RGD site of capsid protein VP1. Analysis of the origin and biological properties of the attenuated strain A22 645 and the results of studies of the primary structure of proteins permit us to hypothesize that attenuation is polygenic, caused by adaptation to a heterologous host (continuous porcine cell culture), and can be expressed by changes in the structure of virus antireceptor providing its binding to cell receptors. Sites responsible for the reproduction of A22 FMDV at certain temperatures are presumably located in RNA polymerase.
Assuntos
Aphthovirus/genética , Sequência de Aminoácidos , Aphthovirus/fisiologia , Sequência de Bases , Capsídeo/genética , Proteínas do Capsídeo , RNA Polimerases Dirigidas por DNA/genética , Dados de Sequência Molecular , MutaçãoRESUMO
A system for detection and strain differentiation of Newcastle disease virus (NDV) by reverse transcription of polymerase chain reaction (RT-PCR) (isolation of RNA, choice of primers for nested PCR, and purification of PCR products) and sequencing is developed and optimized. A nucleotide sequence of gene F site, coding for the F2/F1 cleavage site of F0 fusion protein and including several hypervariable regions, is determined for 10 Russian strains and vaccine strains. The data indicate a replacement of NDV populations in Russia and a rapid evolution of the virus. The origin of pathogenic NDV strains which have been circulating up to the present time is still unknown.
Assuntos
Vírus da Doença de Newcastle/classificação , Sequência de Bases , DNA Bacteriano , Dados de Sequência Molecular , Vírus da Doença de Newcastle/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Homologia de Sequência do Ácido Nucleico , Especificidade da EspécieRESUMO
The complete nucleotide sequence of HN gene, the region of F gene, and intergene regions (M-F, F-HN, and HN-L) of the BOR74 and BOR82 strains of Newcastle disease virus have been determined. Based on the nucleotide and amino acid sequences, the speeds of the nucleic and amino acid changes were calculated (approximately 10(-3) nucleotides or amino acids/year). The BOR strains were grouped phylogenetically with the asymptomatic strains. These strains and the BOR strains have the same motif of the cleavage site (112GKQGR116-L117), but the HN protein of BOR strains has the 572 amino acids which differ the BOR strains from all other strains (571, 577, and 616 amino acids).