RESUMO
The work investigates the potential of peanut shells, an abundant agro-industrial waste, to serve as an adsorbent precursor for the effective and simple treatment of effluents loaded with cadmium and nickel ions. Among the adsorbents prepared, carbonized peanut shell (CCarb), due to its higher adsorption capacity, proved to be the most effective compared to carbonized and activated peanut shell (CATQ). The carbonization process led to structural changes, which resulted in an increase in surface area (around 6 times more in CATQ) and pore volume (around 3 times more in CATQ). Even so, the amount of H+ acid sites due to acid activation produced unfavorable effects for adsorption. Hydroxyl, carboxyl and carbonyl groups were identified on the adsorbent surface which presented favorable charges for metal adsorption. This improvement propels the carbonized variant to the forefront, demonstrating the highest adsorption capacity and reaching equilibrium in less than 90 and 60 min for cadmium and nickel ions, respectively. In both monocomponent and bicomponent systems concentrations greater than 40 ppm signify an increase in adsorption capacity for Ni2+. The experimental data best fit the Freundlich model, showing maximum adsorption capacities of 17.04 mg g-1 for cadmium and 31.28 mg g-1 for nickel. Despite the antagonistic effect observed in the bicomponent system, this study concludes by underlining the promise of activated carbon from peanut shells to harmonize technical and environmental concerns.
Assuntos
Níquel , Poluentes Químicos da Água , Cádmio , Arachis , Adsorção , Bismuto , Íons , Cinética , Poluentes Químicos da Água/análise , Concentração de Íons de HidrogênioRESUMO
INTRODUCTION: Neuropsychiatric diseases are responsible for one of the highest burden of morbidity and mortality worldwide. These illnesses include schizophrenia, bipolar disorder, and major depression. Individuals affected by these diseases may present mitochondrial dysfunction and oxidative stress. Additionally, patients also have increased peripheral and neural chronic inflammation. The Brazilian fruit, açaí, has been demonstrated to be a neuroprotective agent through its recovery of mitochondrial complex I activity. This extract has previously shown anti-inflammatory effects in inflammatory cells. However, there is a lack of understanding of potential anti-neuroinflammatory mechanisms, such as cell cycle involvement. OBJECTIVE: The objective of this study is to evaluate the anti-neuroinflammatory potential of an açaí extract in lipopolysaccharide-activated BV-2 microglia cells. METHODS: Açaí extract was produced and characterized through high performance liquid chromatography. Following açaí extraction and characterization, BV-2 microglia cells were activated with LPS and a dose-response curve was generated to select the most effective açaí dose to reduce cellular proliferation. This dose was then used to assess reactive oxygen species (ROS) production, double-strand DNA release, cell cycle modulation, and cytokine and caspase protein expression. RESULTS: Characterization of the açaí extract revealed 10 bioactive molecules. The extract reduced cellular proliferation, ROS production, and reduced pro-inflammatory cytokines and caspase 1 protein expression under 1 µg/mL in LPS-activated BV-2 microglia cells but had no effect on double strand DNA release. Additionally, açaí treatment caused cell cycle arrest, specifically within synthesis and G2/Mitosis phases. CONCLUSION: These results suggest that the freeze-dried hydroalcoholic açaí extract presents high anti-neuroinflammatory potential.
Assuntos
Euterpe , Microglia , Extratos Vegetais , Animais , Linhagem Celular , Citocinas/metabolismo , Euterpe/química , Lipopolissacarídeos , Camundongos , Microglia/efeitos dos fármacos , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Espécies Reativas de Oxigênio/metabolismoRESUMO
Morus nigra L. is a plant popularly known as 'amoreira preta', very used in folk medicine. Iron overload (hemochromatosis) is a clinical condition that causes damage to various tissues due to oxidative stress. Therapy to control iron overload is still unsatisfactory. The protective effect on oxidative stress induced by iron overload was verified. Phytochemical characterization was evaluated by UHPLC-MS/MS. The in silico toxicity predictions of the main phytochemicals were performed via computer simulation. To induce iron overload, the animals received iron dextran (50 mg/kg/day). The test groups received doses of 500 and 1000 mg/kg of M. nigra extract for six weeks. Body weight, organosomatic index, serum iron, hepatic markers, cytokines, interfering factors in iron metabolism, enzymatic and histopathological evaluations were analyzed. Vanillic acid, caffeic acid, 6-hydroxycoumarin, p-coumaric acid, ferulic acid, rutin, quercitrin, resveratrol, apigenin and kaempferol were identified in the extract. In addition, in silico toxic predictions showed that the main compounds presented a low probability of toxic risk. The extract of M. nigra showed to control the mediators of inflammation and to reduce iron overload in several tissues. Our findings illustrate a novel therapeutic action of M. nigra leaves on hemochromatosis caused by iron overload.
Assuntos
Hemocromatose , Sobrecarga de Ferro , Morus , Animais , Morus/química , Morus/metabolismo , Quempferóis/análise , Quempferóis/farmacologia , Resveratrol/farmacologia , Hemocromatose/tratamento farmacológico , Apigenina/análise , Apigenina/farmacologia , Ácido Vanílico/farmacologia , Espectrometria de Massas em Tandem , Simulação por Computador , Dextranos/análise , Dextranos/metabolismo , Dextranos/farmacologia , Extratos Vegetais/uso terapêutico , Folhas de Planta/química , Estresse Oxidativo , Sobrecarga de Ferro/prevenção & controle , Compostos Fitoquímicos/análise , Rutina/farmacologia , Ferro/toxicidade , Ferro/análise , Citocinas/metabolismo , Mediadores da Inflamação/metabolismoRESUMO
The solution mixture of the non-steroidal anti-inflammatory drugs ketoprofen, meloxicam and tenoxicam was degraded through systems, composed of different photocatalysts based on TiO2 (Fe and Cu) and the hydrogen peroxide oxidant. The monitoring was performed by UV-Vis spectroscopy. Under sunlight radiation, a reduction in peaks was observed with the use of impregnated photocatalysts. After 60 min, the sun/H2O2/Fe-TiO2 system reached degradations of 46.5% and 93.2% at 260 and 367 nm, respectively, and was selected for further studies. The degradation kinetic reached 92 and 96% of degradation after 180 min, for the λ of 260 and 367 nm, respectively. The kinetic curve could be represented by the empirical model proposed by Nichela and co-authors, indicating that besides the heterogeneous photocatalysis that occurs at the surface of the TiO2 there is also the joint effect of the photo-Fenton process. After the treatment, there was no toxicity to cress and lettuce seeds. However, a sensitivity of the thyme seeds to the compounds formed during the treatment was verified. After the fifth treatment cycle, the supported photocatalyst showed degradation higher than 82%. These results indicate that this system is suitable for the treatment of effluents containing pharmaceutical compounds.
Assuntos
Cetoprofeno , Preparações Farmacêuticas , Poluentes Químicos da Água , Catálise , Peróxido de Hidrogênio , Meloxicam , Oxirredução , Piroxicam/análogos & derivados , Poliestirenos , Titânio , Raios UltravioletaRESUMO
Since conventional processes for treating textile effluents have limitations, this work aimed to investigate the application of advanced oxidation technology in this type of matrix. Initially, for a textile dyes mixture in solution, the photo-Fenton/sunlight process proved to be the most efficient among other systems tested. During the tests it was found that the degradation kinetics depends of the pH and catalyst and oxidant concentrations. After 60 min under optimized conditions, the color was reduced by 98.19%, with 92.52% organic matter conversion. Ecotoxicity tests with the Lactuca Sativa vegetable indicated that the dyes were not totally oxidized to inert compounds, although the treated solution did not cause a significant toxic effect for this species. In the second stage of the research, the photodegradation in real samples of textile wastewater was evaluated. The efficiency of the photo-Fenton/sunlight process was lower than that obtained for the dyes solution, a fact attributed to the greater complexity of the real matrix. However, the data also indicated that the combination of coagulation/flocculation and advanced oxidation processes is the most suitable methodology to reduce the fraction of biodegradable compounds. In summary, research has revealed that photocatalytic degradation of dyes through advanced oxidation is an efficient treatment.
Assuntos
Águas Residuárias , Poluentes Químicos da Água , Peróxido de Hidrogênio , Ferro , Cinética , Oxirredução , Têxteis , Eliminação de Resíduos Líquidos , Poluentes Químicos da Água/toxicidadeRESUMO
INTRODUCTION: Nitric oxide is a gaseous radical produced by the nitric oxide endothelial synthase (eNOS) whose most studied physiological action is the vasodilation. However, it also acts in the defense of the organism through the formation of cytotoxic radicals, which can potentiate the inflammatory lesion of the cells. The Glu298Asp is a single nucleotide polymorphism (SNP) in the eNOS gene related to the risk of cardiovascular disease. Blacks present a higher prevalence of hypertension and cardiovascular mortality. Then, we aimed to evaluate the influence of Glu298Asp polymorphism on inflammatory response in vitro and gene expression in blacks. MATERIAL AND METHODS: Peripheral blood mononuclear cells (PBMC) from blacks with different Glu298Asp genotypes were treated with phytohemagglutinin (PHA), a mitogen and activator of T cells. Oxidative, inflammatory markers, and expression of inflammation genes were evaluated. RESULTS: The genotype frequencies were TT 6.7%; TG 29.3% and GG 64.0%. Activation of PBMCs with 125⯵g of PHA modulated the expression of inflammatory genes and increased levels of inflammatory cytokines. The T allele showed increased susceptibility to inflammation (higher levels of interleukin 1, interleukin 6 and tumor necrosis factor alpha; pâ¯<â¯0.001). The G allele exhibited protection through higher levels of nitric oxide (pâ¯<â¯0.001) and fewer inflammatory cytokines. CONCLUSION: Despite methodological limitations related to in vitro assays, the whole of results suggested that Glu298Asp modulates inflammatory genes, the T allele is more susceptible to inflammation and the G allele is protective.
Assuntos
Citocinas/metabolismo , Leucócitos Mononucleares/metabolismo , Óxido Nítrico Sintase Tipo III/genética , Alelos , População Negra/genética , Estudos de Associação Genética , Genótipo , Humanos , Inflamação/genética , Inflamação/metabolismo , Interleucina-1/metabolismo , Interleucina-6/metabolismo , Leucócitos Mononucleares/efeitos dos fármacos , Leucócitos Mononucleares/imunologia , Mitógenos/farmacologia , Óxido Nítrico/metabolismo , Fenótipo , Fito-Hemaglutininas/farmacologia , Polimorfismo de Nucleotídeo Único , Fator de Necrose Tumoral alfa/metabolismoRESUMO
This work investigates the efficiency of LED and UV-C photo-reactors for paracetamol degradation using advanced oxidative processes. Among the evaluated processes, photo-Fenton was the most efficient for both radiations. Degradations greater than 81% (λ 197 nm) and 91% (λ 243 nm) were obtained in the kinetic study. These degradations were also observed by means of the reduction in the peaks in both spectral scanning and high-performance liquid chromatography analysis. The good fit of the Chan and Chu kinetic model shows that the degradation reaction has pseudo-first order behavior. Toxicity tests did not indicate the inhibition of growth of Lactuca sativa seeds and Escherichia coli bacterium. However, the growth of strains of the Salmonella enteritidis bacterium was inhibited in all the samples, demonstrating that only this bacterium was sensitive to solutions. The proposed empirical models obtained from the 24 factorial designs were able to predict paracetamol degradation. These models could, at the same levels assessed, be used to predict the percentage of degradation in studies using other organic compounds. The LED and UV-C photo-reactors were, when employing the photo-Fenton process, able to degrade paracetamol, thus highlighting the efficiency of LED radiation when its power (three times smaller) is compared to that of UV-C radiation.
Assuntos
Acetaminofen , Poluentes Químicos da Água , Peróxido de Hidrogênio , Ferro , Oxirredução , Raios UltravioletaRESUMO
Traumatic spinal cord injury (SCI) promotes long-term disability that affects mobility and functional independence. The spinal cord inflammatory response after the initial mechanical insult substantially impacts locomotor impairment and development of neuropsychiatric disorders, including anxiety and depression. However, these psychiatric events are scarcely investigated in females. This study investigated the anxiety/depression-like behaviours and inflammatory responses related to the production/release of pro- and anti-inflammatory cytokines in female adult Wistar rats submitted to severe clip-compression SCI. Data showed that SCI impaired the locomotor performance assessment by the BBB scale, but did not alter exploratory activity in open-field test. Animals' locomotor impairment was associated with anxious and depressive-like behaviours characterised by a decreased amount of time in the open arms of the elevated plus-maze test, and the motivational reduction of social interaction and anhedonia assessed by social exploration and sucrose preference tests. By contrast, SCI decreased the immobility time in the forced swimming test. Moreover, SCI caused a significant increase in local and systemic proinflammatory cytokines (TNF-α, INF-γ, IL-1ß, and IL-6) and a reduction in the anti-inflammatory cytokine IL-10. Finally, there were significant negative correlations between depression-like behaviour, but not anxiety, and increased plasma concentrations of TNF-α, IL-1ß, IL-6, and INF-γ. Additionally, the laminectomy procedure provoked the inflammatory response associated with reduced sucrose intake in Sham animals, although less expressively than in the SCI group. Collectively, these results indicate that SCI by clip-compression in female rats promotes a neuropsychiatric-like profile associated with an imbalance in the production/release of pro- and anti-inflammatory cytokines.
Assuntos
Ansiedade/imunologia , Depressão/imunologia , Traumatismos da Medula Espinal/psicologia , Animais , Transtornos de Ansiedade/complicações , Comportamento Animal , Citocinas , Transtorno Depressivo/complicações , Modelos Animais de Doenças , Feminino , Inflamação/complicações , Ratos , Ratos Wistar , Medula Espinal/fisiopatologia , Traumatismos da Medula Espinal/complicações , Traumatismos da Medula Espinal/imunologia , Fator de Necrose Tumoral alfaRESUMO
Mancozeb (MZ), chlorothalonil (CT), and thiophanate methyl (TM) are pesticides commonly used in agriculture due to their efficacy, low acute toxicity to mammals, and short environmental persistence. Although the toxic effects of these pesticides have been previously reported, studies regarding their influence on the immune system are limited. As such, this study focused on the immunomodulatory effect of MZ, CT, and TM pesticides on macrophage cells. RAW 264.7â¯cells were exposed to a range of concentrations (0.1-100⯵g/mL) of these pesticides. CT exposure promoted an increase in reactive oxygen species (ROS) and nitric oxide (NO) levels. The MTT and ds-DNA assay results demonstrated that MZ, CT, and TM exposure induced macrophage proliferation. Moreover, MZ, CT, and TM promoted cell cycle arrest at S phase, strongly suggesting macrophage proliferation. The levels of pro-inflammatory cytokines (IL-1ß, IL-6, TNF-α, and IFN-γ) and caspases (caspase 1, 3, and 8) in macrophages exposed to MZ, CT, and TM pesticides increased, whereas the anti-inflammatory cytokine levels decreased. These results suggest that MZ, CT, and TM exert an immunomodulatory effect on the immune system, inducing macrophage activation and enhancing the inflammatory response.
Assuntos
Praguicidas/toxicidade , Animais , Citocinas/metabolismo , Imunomodulação , Interleucina-1beta/metabolismo , Macrófagos/efeitos dos fármacos , Maneb/toxicidade , Óxido Nítrico/metabolismo , Nitrilas/toxicidade , Espécies Reativas de Oxigênio/metabolismo , Tiofanato/toxicidade , Testes de Toxicidade , Fator de Necrose Tumoral alfa/metabolismo , Zineb/toxicidadeRESUMO
Skin aging is a complex biological process induced by intrinsic and extrinsic factors which is characterized by clinical and cellular changes, especially dermal fibroblasts. It is possible that, some procedures, such as low-level laser therapy (LLLT), could decelerate this process. To test this hypothesis, this study evaluated the in vitro LLLT on dermal fibroblast cell line (HFF-1) with premature senescence H2O2-induced. HFF-1 cells were cultured in standardized conditions, and initially H2O2 exposed at different concentrations. Fibroblasts were also just exposed at different LLLT (660 nm) doses. From these curves, the lowest H2O2 concentration that induced indicators of premature senescence and the lowest LLLT doses that triggered fibroblast proliferation were used in all assays. Cellular mortality, proliferation, and the levels of oxidative, inflammatory cytokines, apoptotic markers, and of two growth signaling molecules (FGF-1 and KGF) were compared among treatments. The H2O2 at 50 µM concentration induced some fibroblast senescence markers and for LLLT, the best dose for treatment was 4 J (p < 0.001). The interaction between H2O2 at 50 µM and LLLT at 4 J showed partially reversion of the higher levels of DNA oxidation, CASP 3, CASP 8, IL-1B, IL-6, and INFy induced by H2O2 exposure. LLLT also trigger increase of IL-10 anti-inflammatory cytokine, FGF-1 and KGF levels. Cellular proliferation was also improved when fibroblasts treated with H2O2 were exposed to LLLT (p < 0.001). These results suggest that in fibroblast with some senescence characteristics H2O2-induced, the LLLT presented an important protective and proliferative action, reverting partially or totally negative effects triggering by H2O2.
Assuntos
Apoptose/efeitos da radiação , Biomarcadores/metabolismo , Senescência Celular/efeitos da radiação , Derme/patologia , Fibroblastos/efeitos dos fármacos , Fibroblastos/efeitos da radiação , Peróxido de Hidrogênio/toxicidade , Terapia com Luz de Baixa Intensidade , Antioxidantes/metabolismo , Caspase 3/metabolismo , Caspase 8/metabolismo , Ciclo Celular/efeitos dos fármacos , Ciclo Celular/efeitos da radiação , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Senescência Celular/efeitos dos fármacos , Citocinas/metabolismo , DNA/metabolismo , Fibroblastos/metabolismo , Fibroblastos/patologia , HumanosRESUMO
This work investigated the efficiency of polyethylene terephthalate (PET) as support material for TiO2 films in the photocatalytic degradation of red Bordeaux and yellow tartrazine dyes. The optimum operating conditions were determined by a factorial design, which resulted after 180 min of treatment in degradations of 99.5% and 99.1% for the UVC/H2O2/TiO2Sup and solar/H2O2/TiO2Sup systems, respectively. For the kinetic study, the experimental data fitted to the pseudo-first-order model and the calculated kinetic constants (k) values were 0.03 min-1 for the UVC/H2O2/TiO2Sup system and 0.0213 min-1 for the system solar/H2O2/TiO2Sup. It was verified that TiO2 supported in the PET remained with high degradation efficiency even after five cycles of reuse, indicating a good stability of the photocatalyst in the support. A significant reduction of TOC content was also observed along the reaction time. The phytotoxicity bioassay with Lactuca sativa demonstrated that after treatment with UVC/H2O2/TiO2Sup and solar/H2O2/TiO2SUP, an increase in IC50 and consequently lower toxicity was observed.
Assuntos
Corantes/química , Modelos Químicos , Redes Neurais de Computação , Polietilenotereftalatos/química , Poluentes Químicos da Água/química , Catálise , Corantes/análise , Peróxido de Hidrogênio , Cinética , Oxirredução , Titânio , Raios Ultravioleta , Poluentes Químicos da Água/análise , Purificação da Água/métodosRESUMO
Antipsychotic drugs are used to treat schizophrenia and other psychiatric disorders. However, most of these drugs present side effects causing obesity and other serious metabolic alterations that correlate with grade of chronic inflammation. In contrast, ziprasidone's (ZIP) metabolic side effects are attenuated relative to those of other antipsychotic drugs, but some reports suggest that this drug could cause allergic, hypersensitive reactions in susceptible patients. At present, the mechanism of ZIP's effect on peripheral inflammatory metabolism is not well characterized. We conducted an in vitro study to evaluate the effect of ZIP on a macrophage cell line (RAW 264.1). Our results showed that in non-activated macrophage cells, ZIP exposure initiated macrophage spreading; increased cellular proliferation, as evaluated by MTT and flow cytometry assays; and presented higher levels of oxidant molecules involved in the inflammatory response (nitric oxide, superoxide, reactive oxygen species), and proinflammatory cytokines (IL-1, IL-6, TNFα, INFγ). Levels of IL-10, an anti-inflammatory cytokine were lower in ZIP-exposed cells. These effects were less potent than those caused by the positive control for inflammation induction (phytohemagglutinin), and more intense than the effects of lithium (LI), which was used as an anti-inflammatory molecule. ZIP also modulated cytokine gene expression. Taken together, these data suggest that ZIP can produce a peripheral inflammatory response, and this response may explain the allergen-inflammatory response observed in some patients treated with this antipsychotic drug.
Assuntos
Antipsicóticos/efeitos adversos , Inflamação/induzido quimicamente , Inflamação/patologia , Macrófagos/patologia , Piperazinas/efeitos adversos , Tiazóis/efeitos adversos , Animais , Antipsicóticos/química , Biomarcadores/metabolismo , Ciclo Celular/efeitos dos fármacos , Citocinas/metabolismo , Macrófagos/efeitos dos fármacos , Camundongos , Oxirredução , Piperazinas/química , Células RAW 264.7 , Tiazóis/químicaRESUMO
Temporal lobe epilepsy (TLE) is the most frequent and medically refractory type of epilepsy in humans. In addition to seizures, patients with TLE suffer from behavioral alterations and cognitive deficits. Poststatus epilepticus model of TLE induced by pilocarpine in rodents has enhanced the understanding of the processes leading to epilepsy and thus, of potential targets for antiepileptogenic therapies. Clinical and experimental evidence suggests that inflammatory processes in the brain may critically contribute to epileptogenesis. Statins are inhibitors of cholesterol synthesis, and present pleiotropic effects that include antiinflammatory properties. We aimed the present study to test the hypothesis that atorvastatin prevents behavioral alterations and proinflammatory state in the early period after pilocarpine-induced status epilepticus. Male and female C57BL/6 mice were subjected to status epilepticus induced by pilocarpine and treated with atorvastatin (10 or 100mg/kg) for 14days. Atorvastatin slightly improved the performance of mice in the open-field and object recognition tests. In addition, atorvastatin dose-dependently decreased basal and status epilepticus-induced levels of interleukin-1ß (IL-1ß), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and interferon-γ (INF-γ) and increased interleukin-10 (IL-10) levels in the hippocampus and cerebral cortex. The antiinflammatory effects of atorvastatin were qualitatively identical in both sexes. Altogether, these findings extend the range of beneficial actions of atorvastatin and indicate that its antiinflammatory effects may be useful after an epileptogenic insult.
Assuntos
Atorvastatina/farmacologia , Epilepsia/tratamento farmacológico , Hipocampo/metabolismo , Pilocarpina/toxicidade , Estado Epiléptico/tratamento farmacológico , Animais , Anti-Inflamatórios/farmacologia , Atorvastatina/uso terapêutico , Córtex Cerebral/patologia , Transtornos Cognitivos , Convulsivantes/farmacologia , Modelos Animais de Doenças , Feminino , Regulação da Expressão Gênica/efeitos dos fármacos , Hipocampo/efeitos dos fármacos , Hipocampo/patologia , Humanos , Interleucina-1beta/efeitos dos fármacos , Interleucina-6/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Pilocarpina/farmacologia , Convulsões/tratamento farmacológico , Estado Epiléptico/induzido quimicamenteRESUMO
The study evaluated the advanced oxidative processes concerning the degradation of green leaf and purple açaí dyes, as well as the prediction of data through artificial neural networks (ANNs). It was verified that percentage of degradation on the wavelengths (λ) of 215, 248, 523 and 627 nm was 5.95, 49.99, 98.17 and 95.99%, respectively, when UV/H2O2 action and UV-C radiation was applied. A non-linear kinetic model proposed by Chan and Chu presented a good fit to the experimental data, reaching an R2 value between 0.978 and 0.999, for the studied λ. Within the ANN simulations through Statistica 6.0, the multilayer perceptron (MLP) (3-9-4) presented a better fit to the experimental data. However, higher values of R² were obtained when utilizing the sklearn package with Python language and an MLP (4-5-4) model. Assays with Staphylococcus aureus and Staphylococcus pyogenes bacteria isolates were performed and it was verified that after employing the UV/H2O2 process, there was a decrease in the toxicity of the solution of dyes. In evaluating S. aureus toxicity, normal growth was observed. However, for S. pyogenes bacteria, it was found that when using the UV/H2O2 process, toxicity was evidenced at post-treatment solution concentrations of 100, 70 and 50%.
Assuntos
Corantes/química , Peróxido de Hidrogênio/química , Redes Neurais de Computação , Corantes/toxicidade , Humanos , Cinética , Oxirredução , Estresse Oxidativo , Folhas de Planta , Staphylococcus/efeitos dos fármacos , Raios Ultravioleta , Purificação da ÁguaRESUMO
In this work, the degradation of Remazol Yellow Gold RNL-150% and Reactive Turquoise Q-G125 were investigated using AOP: photolysis, UV/H2O2, Fenton and photo-Fenton. It was found that the photo-Fenton process employing sunlight radiation was the most efficient, obtaining percentages of degradation above 87%. The ideal conditions for the degradation of the dyes were determined from a factorial design 23 and study of the [H2O2] ([H2O2] equal to 100 mg·L-1); [Fe] equal to 1 mg·L-1 and pH between 3 and 4. In the kinetic study, a degradation of more than 97% was obtained after 150 min for the chromophoric groups and 91% for the aromatic compounds. The experimental data obtained presented a good fit to the nonlinear kinetic model. The model of artificial neural networks multilayer perceptron (MLP) (4-11-5) using the software Statistica 8.0 enabled the modeling of the degradation process and showed a better prediction of the data. The toxicity to the seeds of Lactuca sativa and the bacteria Escherichia coli and Salmonella enteritidis allowed to evaluate the effectiveness of the process. The results of this study suggest that the use of photo-Fenton process with sunlight radiation is an effective way to degrade the dyes under study.
Assuntos
Compostos Azo/química , Corantes/química , Metaloporfirinas/química , Redes Neurais de Computação , Ácidos Sulfanílicos/química , Poluentes Químicos da Água/química , Escherichia coli/efeitos dos fármacos , Peróxido de Hidrogênio/química , Ferro/química , Lactuca/efeitos dos fármacos , Oxirredução , Fotólise , Salmonella enteritidis/efeitos dos fármacos , Luz Solar , Poluentes Químicos da Água/toxicidadeRESUMO
This study aimed to assess whether lipid-inflammatory-oxidative metabolism influences auditory processing skills, and whether they function in changing auditory performance after hearing aid fitting in the elderly. Twelve subjects with bilateral hearing loss were submitted to blood tests (to check their lipid-inflammatory-oxidative metabolism) and auditory processing skill tests. After 3 months of using the hearing aids, their auditory skills were re-evaluated and the data were correlated statistically. Oxidative stress levels mainly showed some impact on auditory temporal processing; such a relation and others should best be examined in further studies with larger populations.
Assuntos
Auxiliares de Audição , Perda Auditiva Bilateral/metabolismo , Perda Auditiva Neurossensorial/metabolismo , Metabolismo dos Lipídeos/fisiologia , Estresse Oxidativo/fisiologia , Percepção da Fala/fisiologia , Idoso , Feminino , Perda Auditiva Bilateral/reabilitação , Perda Auditiva Neurossensorial/reabilitação , Testes Auditivos , Humanos , Masculino , Pessoa de Meia-IdadeRESUMO
Antipsychotic drugs have been used in the treatment of schizophrenia and their long-term use can cause movement disorders, such as tardive dyskinesia (TD) in humans mainly typical ones such as haloperidol. Neuroinflammation has been implicated to the use of antipsychotics besides its participation in TD remains unclear. Thus, the aim of this study was to investigate the relation of cytokines with vacuous chewing movements (VCMs) in rats comparing typical and atypical antipsychotics. Rats were treated with haloperidol or risperidone for 28 days. On day 29, rats were subjected to behavioral analysis (quantification of crossing and rearing numbers and VCMs) with subsequent measurement of cytokines levels in the striatum. Haloperidol, but not risperidone treatment significantly decreased the number of crossing and rearing and increased the VCMs when compared with control group. Both antipsychotics were able to increase the levels of pro-inflammatory cytokines (IL-1ß, IL-6, TNF-α and IFN-γ) and decrease the anti-inflammatory cytokine (IL-10) in striatum of rats. However, IL-1ß and IFN-γ levels were higher in animals treated with haloperidol than risperidone. Furthermore, positive correlations were observed between the cytokines (IL-1ß and IFN-γ) and VCM numbers. Thus, the results suggest a role of inflammatory markers in the development of movement disorders, especially IL-1ß and IFN-γ.
Assuntos
Antipsicóticos/farmacologia , Comportamento Animal/efeitos dos fármacos , Corpo Estriado/efeitos dos fármacos , Citocinas/metabolismo , Haloperidol/farmacologia , Animais , Corpo Estriado/metabolismo , Masculino , Transtornos dos Movimentos/tratamento farmacológico , Ratos Wistar , Risperidona/farmacologiaRESUMO
Resveratrol is an molecule that provides both anti-inflammatory and antioxidant properties. However, it is unclear whether the basal oxidative state of the cell has any influence on the effects of this compound. In humans, a single nucleotide polymorphism (SNP) is present in the enzyme manganese superoxide dismutase (SOD2), localized in codon 16 (rs4880), which can either be an alanine (A) or valine (V). This SNP causes an imbalance in the cellular levels of SOD2, where AA- and VV-genotypes result in higher or lower enzymatic activity, respectively. Furthermore, the VV-genotype has been associated with high levels of inflammatory cytokines. Here, we examined the effects of a range of resveratrol concentrations on the in vitro activation of human peripheral blood mononuclear cells (PBMCs) carrying different Ala16Val-SOD2 genotypes. Cell proliferation, several oxidative biomarkers and cytokines (IL-1ß, IL-6, TNFα, Igγ and IL-10) were analyzed. In addition, the effects of resveratrol on the expression of the sirt1 gene were evaluated by qRT-PCR. After 24 h exposure to resveratrol, A-genotype PBMCs displayed a decrease in cell proliferation, whilst VV-cells contrasted; At 10 µM resveratrol, there was a significant decrease in the production of inflammatory cytokines in A-allele cells; however, VV-cells generally displayed a subtle decrease in these, except for TNFα, which was not affected. In all SOD2 genotypes cells exposed to resveratrol resulted in an upregulation of Sirt1 levels. Together, these results suggest that the effect of resveratrol on human PBMC activation is not universal and is dependent on the Ala16Val-SOD2 SNP.
Assuntos
Anti-Inflamatórios/farmacologia , Leucócitos Mononucleares/efeitos dos fármacos , Polimorfismo de Nucleotídeo Único , Estilbenos/farmacologia , Superóxido Dismutase/genética , Antioxidantes/farmacologia , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Citocinas/metabolismo , Relação Dose-Resposta a Droga , Genótipo , Humanos , Mediadores da Inflamação/metabolismo , Leucócitos Mononucleares/enzimologia , Leucócitos Mononucleares/imunologia , Estresse Oxidativo/efeitos dos fármacos , Fenótipo , Resveratrol , Sirtuína 1/genética , Sirtuína 1/metabolismo , Superóxido Dismutase/metabolismoRESUMO
This work proposes the use of agro-industrial wastes, specifically peanut hull (HP) and orange peel (OP), as adsorbents for dyes, such as Remazol Golden Yellow RNL-150% (RYG), Gray Reactive BF-2R (RG) and Reactive Turquoise Q-G125 (RT). Characterization by Brunauer-Emmett-Teller indicates that the adsorbents are mesoporous, with pHzpc values of 5.0 for HP and 4.0 for OP. Fourier transform-infrared spectroscopy identified carbonyl and sulphonic groups. The initial pH of the best-adsorbing solution of the three colours was 2.0. Increasing the concentration of the adsorbent promoted an increase in the percentage of removal until saturation of the adsorbent. In a factorial design, the largest value of q was obtained with 0.25 g of the adsorbent, with a particle size of < 0.4 mm and a stirring speed of 300 rpm. Such conditions were used in kinetic studies and studies of adsorption equilibrium. The evolution kinetics were rapid in the first few minutes, and after 180 min the system reached equilibrium. The kinetic model that best fit the experimental data to a 95% confidence level for the F test was the pseudo-second-order model for RYG/HP, RG/OP and RT/OP. There was no significant difference between the kinetic models as evaluated by the F test for RYG/OP, RG/HP and RT/HP. The experimental results indicated favourable dye adsorption characteristics for the adsorbents studied. The results of the F test showed that for RYG and RG, there was no significant difference between the two evaluated models. This study suggests that HP and OP are viable alternatives for the treatment of effluents containing RYG, RG and RT dyes.