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1.
Cell Mol Life Sci ; 81(1): 71, 2024 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-38300320

RESUMO

Hexosylceramides (HexCer) are implicated in the infection process of various pathogens. However, the molecular and cellular functions of HexCer in infectious cycles are poorly understood. Investigating the enveloped virus Uukuniemi (UUKV), a bunyavirus of the Phenuiviridae family, we performed a lipidomic analysis with mass spectrometry and determined the lipidome of both infected cells and derived virions. We found that UUKV alters the processing of HexCer to glycosphingolipids (GSL) in infected cells. The infection resulted in the overexpression of glucosylceramide (GlcCer) synthase (UGCG) and the specific accumulation of GlcCer and its subsequent incorporation into viral progeny. UUKV and several pathogenic bunyaviruses relied on GlcCer in the viral envelope for binding to various host cell types. Overall, our results indicate that GlcCer is a structural determinant of virions crucial for bunyavirus infectivity. This study also highlights the importance of glycolipids on virions in facilitating interactions with host cell receptors and infectious entry of enveloped viruses.


Assuntos
Orthobunyavirus , Glucosilceramidas , Ligação Viral , Lipidômica , Espectrometria de Massas
2.
Methods Mol Biol ; 2824: 67-80, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39039406

RESUMO

RT-qPCR allows the detection of viruses and the monitoring of viral replication. This technique was extensively employed during the SARS-CoV-2 pandemic, where it demonstrated its efficiency and robustness. Here we describe the analysis of Rift Valley fever and Toscana virus infections over time, achieved through the RT-qPCR quantification of the viral genome. We further elaborate on the method to discriminate between genomic and antigenomic viral RNAs by using primers specific for each strand during the reverse transcription step.


Assuntos
RNA Viral , Febre do Vale de Rift , Vírus da Febre do Vale do Rift , Vírus da Febre do Vale do Rift/genética , RNA Viral/genética , Febre do Vale de Rift/virologia , Febre do Vale de Rift/diagnóstico , Humanos , Genoma Viral , Reação em Cadeia da Polimerase em Tempo Real/métodos , Replicação Viral/genética , Animais
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