P03-A138†Comparison of specular and light transmission microscopy for endothelial control of corneas stored in the active storage machine.

PURPOSE: The Active Storage Machine (ASM), designed by Sincler (a company of group Laboratoires Théa) for eyebanks, will be used for long term donor corneas preservation at 31°C before transplantation. In this device, the endothelial cell density (ECD) counting is expected to be performed non-invasively throughout the storage, thus without changing the storage medium nor handling the cornea. To meet these constraints, specular microscopy (SM), also used for cold storage was selected, instead of the standard light transmission microscopy (LTM-NaCl) used in eye banks storing corneas in organ culture at 31-34°C. The purpose of this study is to compare both imaging methods for ECD measurement of corneas preserved in ASM. METHODS: Five human corneas from body donation to Science were preserved in a prototype ASM with 35mmHg in the endothelial chamber, 2.5µl/min of Corneamax® (Eurobio, France) at 31°C for 5 days. The endothelium of the cornea was imaged through the ASM window using the CellChek® D+ SM (Konan Medical, California, United-States) equipped with an add on device at customized stage. The cornea was then immediately removed from the ASM and prepared for standard endothelial assessment (dilation of the intercellular spaces using 0.9% NaCl and light transmission imaging). Finally, the endothelium was stained with alizarine red and trypan blue and observed again with the same microscope, to determine ECD using the referenced method up to now. For each cornea and each observation method, 5 images were acquired: 1 central and 4 paracentral. The SM images were counted with the Konan software. The LTM-NaCl and 'Alizarin Red' counts were performed with a dedicated plugin of ImageJ after microscope calibration. RESULTS: The means ± SD of the ECD calculated for SM, LTM-NaCl and 'Alizarine' images were respectively of 2314 ± 537, 2243 ± 506 and 2354 ± 543 cells/mm2. There was no significant difference between the 3 methods (ANOVA one-way, p-value = 0.1066). The percentage error was -1.7% +/- 3.3% for SM and -4.7 +/- 4.0% for light transmission microscopy. CONCLUSION: Quality control of the endothelium of corneas stored in ASM can be performed non-invasively with a standard eye bank SM. The ECD measured by SM does not differ from that measured by the conventional microscopy technique used until now in organoculture.

The Experience of Adults Bereaved by the Suicide of a Close Elderly Relative: A Qualitative Pilot Study.

Suicide in older persons is a serious issue in many countries. The act of intentionally causing one's own death is often associated with lack of social support, thwarted belongingness, or chronic interpersonal difficulties. Therefore, suicide has a significant interpersonal dimension that can influence those left behind. However, studies that have investigated the impact of older adults' suicide on their family are scarce. The objective of this pilot study was to assess the feasibility of a qualitative research on the psychosocial experience of adults bereaved by the suicide of an elderly relative. This research could recruit three participants (daughter, grand-son, and grand-niece) who had lost to suicide a close family member aged between 75 and 90. The analysis of the content of the semi-structured interviews revealed seven main themes: (1) finding an explanation to the suicide, (2) give meaning to the loss, (3) the emotional processes of mourning, (4) the repercussions of the suicide on the individual and the family, (5) looking for support, (6) the taboo and secrecy of suicide, and (7) perceptions of aging and the end of life. To explain the suicide of their loved one, the bereaved mentioned various factors related to aging, such as loss of autonomy, illness, and fear of placement. Although the older relative was perceived to be approaching death because of his/her age, the suicide was still unexpected and shocking and led to various emotions (shock, anger, and guilt) and to family conflicts. Suicide remains a taboo subject, but the newly legalized medical assistance in dying is seen as a potential solution to suffering in old age. Further investigation is clearly needed on this topic and this pilot study indicates that the main difficulties will lie in the choice of selection criteria for participants and in the recruitment process.