RESUMO
Astaxanthin (AXT) is a xanthophyll carotenoid with reported health benefits. Realizing its potential as a bioactive is challenging because of its extremely poor solubility in water. We describe a method to improve the effective solubility of AXT in water through its physical association with phytoglycogen (PG), which is produced in sweet corn as compact, highly branched nanoparticles. We combine PG in water with AXT in acetone, evaporate the acetone, and lyophilize. The result is an AXT-PG complex that can be readily redispersed in water, resulting in stable aqueous dispersions. By characterizing the UV-vis absorbance due to different aggregation states of AXT in the AXT-PG complex, we determined the maximum loading of AXT onto PG to be â¼10% by mass. Our results demonstrate the promise of using PG as a solubilizing agent for hydrophobic compounds in water.
Assuntos
Interações Hidrofóbicas e Hidrofílicas , Nanopartículas , Solubilidade , Água , Xantofilas , Xantofilas/química , Nanopartículas/química , Água/químicaRESUMO
The deformability, responsiveness, and tunability of soft nanoparticles (NPs) offer unique opportunities to learn about their complex properties and the interactions between particles. In the present study, we provide new insights into the physical properties of phytoglycogen (PG) NPs, which are soft, compact particles with a dendritic architecture that are produced in the kernels of sweet corn. In particular, we study PG NPs modified using acid hydrolysis, which not only reduces their diameter but also alters their stiffness, internal structure, and the interactions between particles in aqueous dispersions. We used steady shear rheology to determine the dependence of the relative zero-shear viscosity ηr of aqueous dispersions of acid-hydrolyzed PG NPs on the effective volume fraction Ïeff, which indicated a reduction in stiffness of the particles relative to that of native PG NPs. We quantified this difference by analyzing the nature of the colloidal glasses formed at high Ïeff. We measured a smaller value of the fragility index m for acid-hydrolyzed PG NP glasses than that for native PG NP glasses, indicating that acid-hydrolyzed PG NPs form stronger glasses and are therefore softer than native PG NPs. Unlike the native PG NPs, we observed a distinctive change in the character of the glass transition of the acid-hydrolyzed PG NPs as Ïeff was increased above Ïeffâ¼1: a crossover in the dependence of ηr on Ïeff from Vogel-Fulcher-Tammann behavior to a more gradual, Arrhenius-like behavior. By expressing the steady shear and oscillatory rheology data in terms of generalized Péclet numbers, we obtained collapse of the data onto master curves. We interpret this result in terms of the acid-hydrolyzed PG NPs predominantly interpenetrating neighboring particles at large Ïeff, for which fluctuations of the outer chains enhance the mobility of the particles and make α-relaxation times τα experimentally accessible.
Assuntos
Nanopartículas , Nanopartículas/química , Reologia , Viscosidade , Água/químicaRESUMO
Phytoglycogen (PG) is a polysaccharide produced in the kernels of sweet corn as soft, highly branched, compact nanoparticles. Its tree-like or dendritic architecture, combined with a high-safety profile, makes PG nanoparticles attractive for use in biological applications, many of which rely on the association or binding of small biomolecules. We have developed a methodology to functionalize surface plasmon resonance (SPR) sensor surfaces with PG nanoparticles, and we demonstrate the utility of the PG-functionalized SPR sensor by measuring the binding affinity of the tetrameric concanavalin A (ConA) protein to both native PG nanoparticles and smaller, softer acid-hydrolyzed PG nanoparticles. We measure comparable values of the equilibrium association constant K for native and acid-hydrolyzed PG, with a slightly smaller value for the acid-hydrolyzed particles that we attribute to unfavorable lateral interactions between the tetrameric subunits of ConA due to the increase in surface curvature of the smaller acid-hydrolyzed PG particles. We also use infrared reflection-absorption spectroscopy (IRRAS) to show that ConA maintains a large fraction of its native conformation, and thus its bioactivity, upon binding to PG, representing an important step toward the realization of PG as a novel bioactive delivery vehicle.
Assuntos
Nanopartículas , Ressonância de Plasmônio de Superfície , Concanavalina A/química , Ressonância de Plasmônio de Superfície/métodos , Nanopartículas/química , Espectrofotometria Infravermelho , ProteínasRESUMO
Phytoglycogen is a naturally occurring glucose polymer that is produced by sweet corn in the form of compact nanoparticles with a dendritic or tree-like architecture. The soft and porous nature of the nanoparticles, combined with their biodegradability and lack of toxicity, makes them ideal for a broad range of applications in personal care, nutrition, and biomedicine. To fully exploit these applications, it is necessary to understand the complex properties of the soft, hydrated nanoparticles in detail. In the present study, we have used atomic force microscopy (AFM) force spectroscopy to collect high-resolution force-distance maps of a large number of individual phytoglycogen nanoparticles, providing unique insights into the morphology and mechanical stiffness of the nanoparticles at the single-particle level. Our measurements performed in water on nanoparticles covalently bonded to gold surfaces revealed an inner branched structure and high deformability of the nanoparticles at modest values of the applied force. These measurements also allowed us to determine the spatial distribution of Young's modulus values within individual nanoparticles. Drying of the nanoparticles resulted in a dramatic increase in Young's modulus, quantifying the effect of hydration on their mechanical stiffness. We obtained excellent agreement between AFM and osmotic pressure measurements of the mechanical properties of hydrated phytoglycogen nanoparticles; the ratio of the average Young's modulus measured using AFM to the bulk modulus measured using osmotic pressure was in close agreement with that expected for a material with Poisson's ratio ν = 0. The soft, deformable nature of phytoglycogen nanoparticles revealed by our measurements provides new insights at the single-nanoparticle level and suggests their suitability for biomedical applications such as transdermal and targeted drug delivery.
Assuntos
Nanopartículas , Módulo de Elasticidade , Fenômenos Mecânicos , Microscopia de Força Atômica , Análise EspectralRESUMO
The interaction of polysaccharides with water has a critical impact on their biological function as well as their technological applications. We performed ellipsometry experiments at different relative humidities (RH) to measure the equilibrium swelling of ultrathin films of different polysaccharides: native and modified phytoglycogen (PG) nanoparticles, dextran, and hyaluronic acid. For RH > 70%, the swelling of hydrophilic polymers with increases in RH is described by hydration forces that are characterized by an exponential decay length λ. Our analysis of the high RH swelling regime allowed us to determine λ and the bulk modulus K of the films of different polysaccharides. We also probed the high RH swelling regime using attenuated total reflection infrared (ATR-IR) spectroscopy, which allowed us to determine the degree of hydrogen bonding of the hydration water within the polysaccharide films. Combining the ellipsometry and ATR-IR spectroscopy results, we find that increases in the order of the hydrogen bond network of the hydration water, as specified by the ATR-IR parameter Rnetwork, lead to linear increases in K and corresponding inverse changes in λ. These measurements help to elucidate the intimate relationships between the degree of ordering of hydration water, hydration forces, and the mechanical stiffness of polysaccharides. For phytoglycogen, the addition of chemical groups, both cationic and anionic, produced significant increases in its water holding capacity and mechanical properties, suggesting that chemical modification can be used to tune the properties of phytoglycogen for different applications.
Assuntos
Polissacarídeos , Água , Ligação de Hidrogênio , Interações Hidrofóbicas e Hidrofílicas , Espectrofotometria InfravermelhoRESUMO
Phytoglycogen is a highly branched polymer of glucose produced as soft, compact nanoparticles by sweet corn. Properties such as softness, porosity, and mechanical integrity, combined with nontoxicity and biodegradability, make phytoglycogen nanoparticles ideal for applications involving the human body, ranging from skin moisturizing and rejuvenation agents in personal care formulations to functional therapeutics in biomedicine. To further broaden the range of applications, phytoglycogen nanoparticles can be chemically modified with hydrophobic species such as octenyl succinic anhydride (OSA). Here, we present a self-consistent model of the particle structure, water content, and degree of chemical modification of the particles, as well as the emergence of well-defined interparticle spacings in concentrated dispersions, based on small-angle neutron scattering (SANS) measurements of aqueous dispersions of native phytoglycogen nanoparticles and particles that were hydrophobically modified using octenyl succinic anhydride (OSA) in both its protiated (pOSA) and deuterated (dOSA) forms. Measurements on native particles with reduced polydispersity have allowed us to refine the particle morphology, which is well described by a hairy particle (core-chain) geometry with short chains decorating the surface of the particles. The isotopic variants of OSA-modified particles enhanced the scattering contrast for neutrons, revealing lightly modified hairy chains for small degrees of substitution (DS) of OSA, and a raspberry particle geometry for the largest DS value, where the OSA-modified hairy chains collapse to form small seeds on the surface of the particles. This refined model of native and OSA-modified phytoglycogen nanoparticles establishes a quantitative basis for the development of new applications of this promising sustainable nanotechnology.
Assuntos
Nanopartículas , Humanos , Interações Hidrofóbicas e Hidrofílicas , Amido , ÁguaRESUMO
Phytoglycogen is a natural polysaccharide produced in the form of dense, 35 nm diameter nanoparticles by some varieties of plants such as sweet corn. The highly-branched, dendrimeric structure of phytoglycogen leads to interesting and useful properties such as softness and deformability of the particles, and a strong interaction with water. These properties make the particles ideal for use as unique additives in personal care, nutrition and biomedical formulations. In the present study, we describe rheology measurements of aqueous dispersions of phytoglycogen nanoparticles. The viscosity of the dispersions remained Newtonian up to large concentrations (â¼20% w/w). For higher concentrations, the zero-shear viscosity increased dramatically, reaching values that exceeded that of the water solvent by six orders of magnitude at a concentration of 30% w/w and were well described by the Vogel-Fulcher-Tammann relation of glassy dynamics. The very large values of the zero-shear viscosity are coupled with significant deformation of the soft nanoparticles. We quantified the softness of the particles by performing osmotic pressure measurements on concentrated dispersions, obtaining a value of 15 kPa for the compressional modulus. For the most concentrated samples, we observed flow at stresses less than the apparent yield stress value determined by fitting the high strain rate data to the Herschel-Bulkley model. This behavior, similar to that of star polymer glasses, suggests the possibility of a hairy colloid particle geometry. Remarkably, phytoglycogen nanoparticles dispersed in water provide a very simple experimental realization of glass-forming dispersions of soft colloidal particles that can be used to validate theoretical models in detail.
Assuntos
Modelos Teóricos , Nanopartículas/química , Polissacarídeos/química , Reologia , ViscosidadeRESUMO
Phytoglycogen is a highly branched polymer of glucose that forms dendrimeric nanoparticles. This special structure leads to a strong interaction with water that produces exceptional properties such as high water retention, low viscosity, and high stability of aqueous dispersions. We have used ellipsometry at controlled relative humidity (RH) to measure the equilibrium swelling of ultrathin films of phytoglycogen, which directly probes the interstitial forces acting within the films. Comparison of the swelling behavior of films of highly branched phytoglycogen to that of other glucose-based polysaccharides shows that the chain architecture plays an important role in determining both the strong, short-range repulsion of the chains at low RH and the repulsive hydration forces at high RH. In particular, the length scale λ0 that characterizes the exponentially decaying hydration forces provides a quantitative, RH-independent measure of film swelling that differs significantly for different glucose-based polysaccharides. By combining ellipsometry with infrared spectroscopy, we have determined the relationship between water structuring and inter-chain separation in the highly branched phytoglycogen nanoparticles, with maintenance of a high degree of water structure as the film swells significantly at high RH. These insights into the structure-hydration relationship for phytoglycogen are essential to the development of new products and technologies based on this sustainable nanomaterial.
Assuntos
Nanopartículas/química , Nanoestruturas/química , Água/química , Espectrofotometria InfravermelhoRESUMO
The physical properties of confined water can differ dramatically from those of bulk water. Hydration water associated with polysaccharides provides a particularly interesting example of confined water, because differences in polysaccharide structure provide different spatially confined environments for water sorption. We have used attenuated total reflection infrared (ATR-IR) spectroscopy to investigate the structure of hydration water in films of three different polysaccharides under controlled relative humidity (RH) conditions. We compare the results obtained for films of highly branched, dendrimer-like phytoglycogen nanoparticles to those obtained for two unbranched polysaccharides, hyaluronic acid (HA), and chitosan. We find similarities between the water structuring in the two linear polysaccharides and significant differences for phytoglycogen. In particular, the results suggest that the high degree of branching in phytoglycogen leads to a much more well-ordered water structure (low density, high connectivity network water), indicating the strong influence of chain architecture on the structuring of water. These measurements provide unique insight into the relationship between the structure and hydration of polysaccharides, which is important for understanding and exploiting these sustainable nanomaterials in a wide range of applications.
Assuntos
Quitosana/química , Glicogênio/química , Ácido Hialurônico/química , Interações Hidrofóbicas e Hidrofílicas , Água/químicaRESUMO
Phytoglycogen is a naturally occurring polysaccharide nanoparticle made up of extensively branched glucose monomers. It has a number of unusual and advantageous properties, such as high water retention, low viscosity, and high stability in water, which make this biomaterial a promising candidate for a wide variety of applications. In this study, we have characterized the structure and hydration of aqueous dispersions of phytoglycogen nanoparticles using neutron scattering. Small angle neutron scattering results suggest that the phytoglycogen nanoparticles behave similar to hard sphere colloids and are hydrated by a large number of water molecules (each nanoparticle contains between 250% and 285% of its mass in water). This suggests that phytoglycogen is an ideal sample in which to study the dynamics of hydration water. To this end, we used quasielastic neutron scattering (QENS) to provide an independent and consistent measure of the hydration number, and to estimate the retardation factor (or degree of water slow-down) for hydration water translational motions. These data demonstrate a length-scale dependence in the measured retardation factors that clarifies the origin of discrepancies between retardation factor values reported for hydration water using different experimental techniques. The present approach can be generalized to other systems containing nanoconfined water.
Assuntos
Glicogênio/química , Nanopartículas/química , Zea mays/química , Coloides/química , Glucose/química , Interações Hidrofóbicas e HidrofílicasRESUMO
Type IV pili (T4P) are very thin protein filaments that extend from and retract into bacterial cells, allowing them to interact with and colonize a broad array of chemically diverse surfaces. The physical aspects that allow T4P to mediate adherence to many different surfaces remain unclear. Atomic force microscopy (AFM) nanoscale pulling experiments were used to measure the mechanical properties of T4P of a mutant strain of Pseudomonas aeruginosa PAO1 unable to retract its T4P. After adhering bacteria to the end of an AFM cantilever and approaching surfaces of mica, gold, or polystyrene, we observed adhesion of the T4P to all of the surfaces. Pulling of single and multiple T4P on retraction of the cantilever from the surfaces could be described using the worm-like chain (WLC) model. Distinct peaks in the measured distributions of the best-fit values of the persistence length Lp on two different surfaces provide strong evidence for close-packed bundling of very flexible T4P. In addition, we observed force plateaus indicating that adhesion of the T4P to both hydrophilic and hydrophobic surfaces occurs along extended lengths of the T4P. These data shed new light, to our knowledge, on T4P flexibility and support a low-affinity, high-avidity adhesion mechanism that mediates bacteria-surface interactions.
Assuntos
Aderência Bacteriana , Fímbrias Bacterianas/química , Pseudomonas aeruginosa/metabolismo , Sequência de Aminoácidos , Fenômenos Biomecânicos , Proteínas de Fímbrias/química , Proteínas de Fímbrias/genética , Fímbrias Bacterianas/metabolismo , Fímbrias Bacterianas/ultraestrutura , Dados de Sequência Molecular , Mutação , Pseudomonas aeruginosa/genéticaRESUMO
In the present work, two different proteomic platforms, gel-based and gel-free, were used to map the matrix and outer membrane vesicle exoproteomes of Pseudomonas aeruginosa PAO1 biofilms. These two proteomic strategies allowed us a confident identification of 207 and 327 proteins from enriched outer membrane vesicles and whole matrix isolated from biofilms. Because of the physicochemical characteristics of these subproteomes, the two strategies showed complementarity, and thus, the most comprehensive analysis of P. aeruginosa exoproteome to date was achieved. Under our conditions, outer membrane vesicles contribute approximately 20% of the whole matrix proteome, demonstrating that membrane vesicles are an important component of the matrix. The proteomic profiles were analyzed in terms of their biological context, namely, a biofilm. Accordingly relevant metabolic processes involved in cellular adaptation to the biofilm lifestyle as well as those related to P. aeruginosa virulence capabilities were a key feature of the analyses. The diversity of the matrix proteome corroborates the idea of high heterogeneity within the biofilm; cells can display different levels of metabolism and can adapt to local microenvironments making this proteomic analysis challenging. In addition to analyzing our own primary data, we extend the analysis to published data by other groups in order to deepen our understanding of the complexity inherent within biofilm populations.
Assuntos
Proteínas de Bactérias/isolamento & purificação , Biofilmes/crescimento & desenvolvimento , Matriz Extracelular/química , Vesículas Extracelulares/química , Proteoma/isolamento & purificação , Pseudomonas aeruginosa/química , Fatores de Virulência/isolamento & purificação , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Membrana Celular/química , Cromatografia Líquida , Géis , Regulação Bacteriana da Expressão Gênica , Anotação de Sequência Molecular , Proteoma/genética , Proteoma/metabolismo , Proteômica/métodos , Pseudomonas aeruginosa/genética , Pseudomonas aeruginosa/metabolismo , Espectrometria de Massas em Tandem , Fatores de Virulência/genética , Fatores de Virulência/metabolismoRESUMO
Dehydrins (group 2 late embryogenesis abundant proteins) are intrinsically-disordered proteins that are expressed in plants experiencing extreme environmental conditions such as drought or low temperature. Their roles include stabilizing cellular proteins and membranes, and sequestering metal ions. Here, we investigate the membrane interactions of the acidic dehydrin TsDHN-1 and the basic dehydrin TsDHN-2 derived from the crucifer Thellungiella salsuginea that thrives in the Canadian sub-Arctic. We show using compression studies with a Langmuir-Blodgett trough that both dehydrins can stabilize lipid monolayers with a lipid composition mimicking the composition of the plant outer mitochondrial membrane, which had previously been shown to induce ordered secondary structures (disorder-to-order transitions) in the proteins. Ellipsometry of the monolayers during compression showed an increase in monolayer thickness upon introducing TsDHN-1 (acidic) at 4°C and TsDHN-2 (basic) at room temperature. Atomic force microscopy of supported lipid bilayers showed temperature-dependent phase transitions and domain formation induced by the proteins. These results support the conjecture that acidic dehydrins interact with and potentially stabilize plant outer mitochondrial membranes in conditions of cold stress. Single-molecule force spectroscopy of both proteins pulled from supported lipid bilayers indicated the induced formation of tertiary conformations in both proteins, and potentially a dimeric association for TsDHN-2.
Assuntos
Brassicaceae/metabolismo , Proteínas de Plantas/fisiologia , Sequência de Aminoácidos , Biofísica/métodos , Membrana Celular/metabolismo , Temperatura Baixa , Dimerização , Lipídeos/química , Microscopia de Força Atômica/métodos , Modelos Estatísticos , Dados de Sequência Molecular , Proteínas de Plantas/metabolismo , Estrutura Secundária de Proteína , Estrutura Terciária de Proteína , Proteínas Recombinantes/química , Propriedades de Superfície , TemperaturaRESUMO
To date, detailed studies of the thickness of coatings using surface plasmon resonance have been limited to samples that are very uniform in thickness, and this technique has not been applied quantitatively to samples that are inherently rough or undergo instabilities with time. Our manuscript describes a significant improvement to surface plasmon resonance imaging (SPRi) that allows this sensitive technique to be used for quantitative tracking of the thickness and roughness of surface coatings that are rough on the scale of tens of nanometers. We tested this approach by studying samples with an idealized, one-dimensional roughness: patterned channels in a thin polymer film. We find that a novel analysis of the SPRi data collected with the plane of incidence parallel to the patterned channels allows the determination of the thickness profile of the channels in the polymer film, which is in agreement with that measured using atomic force microscopy. We have further validated our approach by performing SPRi measurements perpendicular to the patterned channels, for which the measured SPR curve agrees well with the single SPR curve calculated using the average thickness determined from the thickness profile as determined using AFM. We applied this analysis technique to track the average thickness and RMS roughness of cellulose microfibrils upon exposure to cellulolytic enzymes, providing quantitative determinations of the times of action of the enzymes that are of direct interest to the cellulosic ethanol industry.
Assuntos
Nanotecnologia , Ressonância de Plasmônio de Superfície/métodos , Celulose/química , Polimetil Metacrilato/química , Propriedades de SuperfícieRESUMO
The effectiveness of antimicrobial compounds can be easily screened, however their mechanism of action is much more difficult to determine. Many compounds act by compromising the mechanical integrity of the bacterial cell envelope, and our study introduces an AFM-based creep deformation technique to evaluate changes in the time-dependent mechanical properties of Pseudomonas aeruginosa PAO1 bacterial cells upon exposure to two different but structurally related antimicrobial peptides. We observed a distinctive signature for the loss of integrity of the bacterial cell envelope following exposure to the peptides. Measurements performed before and after exposure, as well as time-resolved measurements and those performed at different concentrations, revealed large changes to the viscoelastic parameters that are consistent with differences in the membrane permeabilizing effects of the peptides. The AFM creep deformation measurement provides new, unique insight into the kinetics and mechanism of action of antimicrobial peptides on bacteria.
Assuntos
Peptídeos Catiônicos Antimicrobianos/efeitos dos fármacos , Peptídeos Catiônicos Antimicrobianos/farmacologia , Membrana Celular/efeitos dos fármacos , Pseudomonas aeruginosa/efeitos dos fármacos , Humanos , Fenômenos Mecânicos , Testes de Sensibilidade Microbiana , Microscopia de Força Atômica , Pseudomonas aeruginosa/ultraestruturaRESUMO
High-resolution atomic force microscopy (AFM) was used to image the real-time in situ degradation of crystalline by three types of T. reesei cellulolytic enzymes-TrCel6A, TrCel7A, and TrCel7B-and their mixtures. TrCel6A and TrCel7A are exo-acting cellobiohydrolases processing cellulose fibers from the nonreducing and reducing ends, respectively. TrCel7B is an endoglucanase that hydrolyzes amorphous cellulose within fibers. When acting alone on native cellulose fibers, each of the three enzymes is incapable of significant degradation. However, mixtures of two enzymes exhibited synergistic effects. The degradation effects of this synergism depended on the order in which the enzymes were added. Faster hydrolysis rates were observed when TrCel7A (exo) was added to fibers pretreated first with TrCel7B (endo) than when adding the enzymes in the opposite order. Endo-acting TrCel7B removed amorphous cellulose, softened and swelled the fibers, and exposed single microfibrils, facilitating the attack by the exo-acting enzymes. AFM images revealed that exo-acting enzymes processed the TrCel7B-pretreated fibers preferentially from one specific end (reducing or nonreducing). The most efficient (almost 100%) hydrolysis was observed with the mixture of the three enzymes. In this mixture, TrCel7B softened the fiber and TrCel6A and TrCel7A were directly observed to process it from the two opposing ends. This study provides high-resolution direct visualization of the nature of the synergistic relation between T. reesei exo- and endo-acting enzymes digesting native crystalline cellulose.
Assuntos
Celulases/metabolismo , Celulose/metabolismo , Trichoderma/enzimologia , Celulases/química , Celulases/isolamento & purificação , Celulose/química , Cristalização , Microscopia de Força Atômica , Modelos MolecularesRESUMO
A mixed phospholipid-cholestrol bilayer, with cholera toxin B (CTB) units attached to the monosialotetrahexosylganglioside (GM1) binding sites in the distal leaflet, was deposited on a Au(111) electrode surface. Polarization modulation infrared reflection absorption spectroscopy (PM-IRRAS) measurements were used to characterize structural and orientational changes in this model biological membrane upon binding CTB and the application of the electrode potential. The data presented in this article show that binding cholera toxin to the membrane leads to an overall increase in the tilt angle of the fatty acid chains; however, the conformation of the bilayer remains relatively constant as indicated by the small decrease in the total number of gauche conformers of acyl tails. In addition, the bound toxin caused a significant decrease in the hydration of the ester group contained within the lipid bilayer. Furthermore, changes in the applied potential had a minimal effect on the overall structure of the membrane. In contrast, our results showed significant voltage-dependent changes in the average orientation of the protein α-helices that may correspond to the voltage-gated opening and closing of the central pore that resides within the B subunit of cholera toxin.
Assuntos
Toxina da Cólera/química , Técnicas Eletroquímicas , Gangliosídeos/química , Sítios de Ligação , Eletrodos , Ouro/química , Modelos Moleculares , Espectrofotometria Infravermelho , Propriedades de SuperfícieRESUMO
Hyperspectral infrared (IR) images contain a large amount of highly spatially resolved information about the chemical composition of a sample. However, the analysis of hyperspectral IR imaging data for complex heterogeneous systems can be challenging because of the spectroscopic and spatial complexity of the data. We implement a deep generative modeling approach using a ß-variational autoencoder to learn disentangled representations of the generative factors of variance in a data set of cross-linked polyethylene (PEX-a) pipe. We identify three distinct physicochemical factors of aging and degradation learned by the model and apply the trained model to high-resolution hyperspectral IR images of cross-sectional slices of unused virgin, used in-service, and cracked PEX-a pipe. By mapping the learned representations of aging and degradation to the IR images, we extract detailed information on the physicochemical changes that occur during aging, degradation, and cracking in PEX-a pipe. This study shows how representation learning by deep generative modeling can significantly enhance the analysis of high-resolution IR images of complex heterogeneous samples.