RESUMO
L-selectin is a key lectin essential for leukocyte capture and rolling on vessel walls. Functional adhesion of L-selectin requires a minimal threshold of hydrodynamic shear. Using high temporal resolution videomicroscopy, we now report that L-selectin engages its ligands through exceptionally labile adhesive bonds (tethers) even below this shear threshold. These tethers share a lifetime of 4 ms on distinct physiological ligands, two orders of magnitude shorter than the lifetime of the P-selectin-PSGL-1 bond. Below threshold shear, tether duration is not shortened by elevated shear stresses. However, above the shear threshold, selectin tethers undergo 14-fold stabilization by shear-driven leukocyte transport. Notably, the cytoplasmic tail of L-selectin contributes to this stabilization only above the shear threshold. These properties are not shared by P-selectin- or VLA-4-mediated tethers. L-selectin tethers appear adapted to undergo rapid avidity enhancement by cellular transport, a specialized mechanism not used by any other known adhesion receptor.
Assuntos
Adesão Celular/fisiologia , Quimiotaxia de Leucócito/fisiologia , Endotélio Vascular/metabolismo , Selectina L/metabolismo , Leucócitos/metabolismo , Sítios de Ligação/fisiologia , Linhagem Celular , Humanos , Integrina alfa4beta1/metabolismo , Ligantes , Mucinas/metabolismo , Estresse MecânicoRESUMO
L-selectin is a leukocyte cell-surface protein that facilitates the rolling of leukocytes along the endothelium, a process that leads to leukocyte migration to a site of infection. Preventing L-selectin-mediated rolling minimizes leukocyte adhesion and extravasation; therefore, compounds that inhibit rolling may act as anti-inflammatory agents. To investigate the potential role of multivalent ligands as rolling inhibitors, compounds termed neoglycopolymers were synthesized that possess key structural features of physiological L-selectin ligands. Sulfated neoglycopolymers substituted with sialyl Lewis x derivatives (3',6-disulfo Lewis x or 6-sulfo sialyl Lewis x) or a sulfatide analog (3,6-disulfo galactose) inhibited L-selectin-mediated rolling of lymphoid cells. Functional analysis of the inhibitory ligands indicates that they also induce proteolytic release of L-selectin. Thus, their inhibitory potency may arise from their ability to induce shedding. Our data indicate that screening for compounds that promote L-selectin release can identify ligands that inhibit rolling.
Assuntos
Anti-Inflamatórios/química , Anti-Inflamatórios/farmacologia , Selectina L/efeitos dos fármacos , Migração e Rolagem de Leucócitos/efeitos dos fármacos , Animais , Anti-Inflamatórios/síntese química , Sequência de Carboidratos , Linhagem Celular , Linhagem Celular Tumoral , Regulação para Baixo , Glicoproteínas/química , Glicoproteínas/metabolismo , Glicoproteínas/farmacologia , Humanos , Selectina L/química , Selectina L/metabolismo , Ligantes , Linfócitos/efeitos dos fármacos , Camundongos , Mimetismo Molecular , Dados de Sequência MolecularRESUMO
Cholesterol-enriched lipid microdomains regulate L-selectin signaling, but the role of membrane cholesterol in L-selectin adhesion is unclear. Arrest chemokines are a subset of endothelial chemokines that rapidly activate leukocyte integrin adhesiveness under shear flow. In the absence of integrin ligands, these chemokines destabilize L-selectin-mediated leukocyte rolling. In the present study, we investigated how cholesterol extraction from the plasma membrane of peripheral blood T or B cells affects L-selectin adhesions and their destabilization by arrest chemokines. Unlike the Jurkat T cell line, whose L-selectin-mediated adhesion is cholesterol dependent, in primary human PBLs and in murine B cells and B cell lines, cholesterol depletion did not impair any intrinsic adhesiveness of L-selectin, consistent with low selectin partitioning into lipid rafts in these cells. However, cholesterol raft disruption impaired the ability of two arrest chemokines, CXCL12 and CXCL13, but not of a third arrest chemokine, CCL21, to destabilize L-selectin-mediated rolling of T lymphocytes. Actin capping by brief incubation with cytochalasin D impaired the ability of all three chemokines to destabilize L-selectin rolling. Blocking of the actin regulatory phosphatidylinositol lipid, phosphatidylinositol 4,5-bisphosphate, did not affect chemokine-mediated destabilization of L-selectin adhesions. Collectively, our results suggest that L-selectin adhesions are inhibited by actin-associated, cholesterol-stabilized assemblies of CXCL12- and CXCL13-binding receptors on both T and B lymphocytes. Thus, the regulation of L-selectin by cholesterol-enriched microdomains varies with the cell type as well as with the identity of the destabilizing chemokine.
Assuntos
Adesão Celular/fisiologia , Quimiocinas/metabolismo , Colesterol/metabolismo , Selectina L/metabolismo , Migração e Rolagem de Leucócitos/fisiologia , Microdomínios da Membrana/metabolismo , Actinas/metabolismo , Quimiocina CXCL12 , Quimiocina CXCL13 , Quimiocinas/imunologia , Quimiocinas CXC/imunologia , Quimiocinas CXC/metabolismo , Colesterol/imunologia , Citometria de Fluxo , Imunofluorescência , Humanos , Selectina L/imunologia , Linfócitos/química , Linfócitos/imunologia , Linfócitos/metabolismo , Microdomínios da Membrana/imunologia , Proteínas de Membrana/imunologia , Proteínas de Membrana/metabolismo , Microscopia de FluorescênciaRESUMO
Chemokines presented on specialized endothelial surfaces rapidly up-regulate leukocyte integrin avidity and firm arrest through G(i)-protein signaling. Here we describe a novel, G-protein-independent, down-regulatory activity of apical endothelial chemokines in destabilizing L-selectin-mediated leukocyte rolling. Unexpectedly, this anti-adhesive chemokine suppression of rolling does not involve L-selectin shedding. Destabilization of rolling is induced only by immobilized chemokines juxtaposed to L-selectin ligands and is an energy-dependent process. Chemokines are found to interfere with a subsecond stabilization of selectin tethers necessary for persistent rolling. This is a first indication that endothelial chemokines can attenuate in situ L-selectin adhesion to endothelial ligands at subsecond contacts. This negative feedback mechanism may underlie the jerky nature of rolling mediated by L-selectin in vivo.
Assuntos
Quimiocinas/fisiologia , Selectina L/fisiologia , Linfócitos/citologia , Quimiocinas/metabolismo , Endotélio/metabolismo , Técnica Indireta de Fluorescência para Anticorpo , Proteínas de Ligação ao GTP/metabolismo , HumanosRESUMO
VLA-4 and LFA-1 are the major vascular integrins expressed on circulating lymphocytes. Previous studies suggested that intact cholesterol rafts are required for integrin adhesiveness in different leukocytes. We found the alpha(4) integrins VLA-4 and alpha(4)beta(7) as well as the LFA-1 integrin to be excluded from rafts of human peripheral blood lymphocytes. Disruption of cholesterol rafts with the chelator methyl-beta-cyclodextrin did not affect the ability of these lymphocyte integrins to generate high avidity to their respective endothelial ligands and to promote lymphocyte rolling and arrest on inflamed endothelium under shear flow. In contrast, cholesterol extraction abrogated rapid chemokine triggering of alpha(4)-integrin-dependent peripheral blood lymphocytes adhesion, a process tightly regulated by G(i)-protein activation of G protein-coupled chemokine receptors (GPCR). Strikingly, stimulation of LFA-1 avidity to intercellular adhesion molecule 1 (ICAM-1) by the same chemokines, although G(i)-dependent, was insensitive to raft disruption. Our results suggest that alpha(4) but not LFA-1 integrin avidity stimulation by chemokines involves rapid chemokine-induced GPCR rearrangement that takes place at cholesterol raft platforms upstream to G(i) signaling. Our results provide the first evidence that a particular chemokine/GPCR pair can activate different integrins on the same cell using distinct G(i) protein-associated machineries segregated within defined membrane compartments.
Assuntos
Colesterol/metabolismo , Integrina alfa4beta1/química , Integrinas/química , Antígeno-1 Associado à Função Linfocitária/metabolismo , Actinas/metabolismo , Western Blotting , Adesão Celular , Linhagem Celular , Membrana Celular/metabolismo , Separação Celular , Células Cultivadas , Quimiocinas/metabolismo , Citoesqueleto/metabolismo , Endotélio Vascular/citologia , Citometria de Fluxo , Humanos , Inflamação , Integrina alfa4beta1/metabolismo , Integrinas/metabolismo , Molécula 1 de Adesão Intercelular/metabolismo , Células Jurkat , Leucócitos/metabolismo , Ligantes , Microdomínios da Membrana/metabolismo , Microscopia Confocal , Microscopia de Fluorescência , Modelos Biológicos , Ligação Proteica , Receptores CXCR4/imunologia , Receptores CXCR4/metabolismo , Receptores de Superfície Celular/metabolismo , Proteínas Recombinantes/metabolismo , Transdução de Sinais , Veias Umbilicais/citologiaRESUMO
Selectin counterreceptors are glycoprotein scaffolds bearing multiple carbohydrate ligands with exceptional ability to tether flowing cells under disruptive shear forces. Bond clusters may facilitate formation and stabilization of selectin tethers. L-selectin ligation has been shown to enhance L-selectin rolling on endothelial surfaces. We now report that monoclonal antibodies-induced L-selectin dimerization enhances L-selectin leukocyte tethering to purified physiological L-selectin ligands and glycopeptides. Microkinetic analysis of individual tethers suggests that leukocyte rolling is enhanced through the dimerization-induced increase in tether formation, rather than by tether stabilization. Notably, L-selectin dimerization failed to augment L-selectin-mediated adhesion below a threshold ligand density, suggesting that L-selectin dimerization enhanced adhesiveness only to properly clustered ligand. In contrast, an epidermal growth factor domain substitution of L-selectin enhanced tether formation to L-selectin ligands irrespective of ligand density, suggesting that this domain controls intrinsic ligand binding properties of L-selectin without inducing L-selectin dimerization. Strikingly, at low ligand densities, where L-selectin tethering was not responsive to dimerization, elevated shear stress restored sensitivity of tethering to selectin dimerization. This is the first indication that shear stress augments effective selectin ligand density at local contact sites by promoting L-selectin encounter of immobilized ligand.