RESUMO
In this tutorial review, we will explore recent advances in the construction and application of Förster resonance energy transfer (FRET)-based small-molecule fluorescent probes. The advantages of FRET-based fluorescent probes include: a large Stokes shift, ratiometric sensing and dual/multi-analyte responsive systems. We discuss the underlying energy donor-acceptor dye combinations and emphasise their applications for the detection or imaging of cations, anions, small neutral molecules, biomacromolecules, cellular microenvionments and dual/multi-analyte responsive systems.
Assuntos
Transferência Ressonante de Energia de Fluorescência/métodos , Compostos Inorgânicos/análise , Animais , Transporte Biológico , Melhoramento Biomédico , Técnicas Biossensoriais , Linhagem Celular , Microambiente Celular , Humanos , Íons/análise , Potencial da Membrana Mitocondrial , Microscopia de Fluorescência , Neoplasias/diagnóstico por imagem , Imagem Óptica , Espectrometria de Fluorescência , Propriedades de SuperfícieRESUMO
A novel photodeactivation strategy for controlling gene expression has been developed based on light-induced activation of cAMP response element binding protein (CREB). Light-induced cleavage of the photoresponsive protecting group of an antagonist of CREB binding protein (CBP) results in photocleaved products with weak binding affinity for CBP. This photodissociation reaction enables protein-protein interactions between CBP and CREB that trigger the formation of a multiprotein transcription complex to turn gene expression "on". This enables irradiation of antagonist-treated HEK293T cells to be used to trigger temporal recovery of CREB-dependent transcriptional activity and endogenous gene expression under photolytic control.