RESUMO
Molecular virological studies of the field material collected in the epicenter of epizooty with high mortality among mute swans (Cygnus olor) in the area of the lower estuary of the Volga River (November 2005) could establish the etiological role of highly pathogenic influenza A (HPAI) virus of the subtype H5N1. Ten HPAI/H5N1 strains deposited at the State Collection of Viruses of the Russian Federation with the priority dated December 1, 2005 were isolated from the cloacal/tracheal swabs and viscera of sick and freshly died mute swans. Complete nucleotide sequences of all fragments of the genome of 6 strains have been deposited in the Gene Bank. The paper discusses the molecular genetic characteristics of isolated strains.
Assuntos
Animais Selvagens/virologia , Aves/virologia , Surtos de Doenças , Virus da Influenza A Subtipo H5N1/isolamento & purificação , Influenza Aviária/epidemiologia , Influenza Aviária/virologia , Animais , Sangue/virologia , Linhagem Celular , Cloaca/virologia , Cães , Genes Virais , Virus da Influenza A Subtipo H5N1/genética , Dados de Sequência Molecular , Proteínas do Nucleocapsídeo , Nucleoproteínas/genética , Orthomyxoviridae/genética , Proteínas de Ligação a RNA/genética , RNA Polimerase Dependente de RNA/genética , Federação Russa/epidemiologia , Suínos , Traqueia/virologia , Proteínas do Core Viral/genética , Proteínas da Matriz Viral/genética , Proteínas não Estruturais Virais/genética , Proteínas Virais/genética , Vísceras/virologiaRESUMO
Ninety patients with aphakia and diabetes were examined. Intraocular pressure was normal in 30 patients, in 30 aphakia was concomitant with primary glaucoma, and 30 presented with aphakic glaucoma. Fundus oculi was examined with a slit lamp and +60 D lens, gonioscopy and cycloscopy were carried out using a contact prism proposed by the authors. Aphakic glaucoma developed as a result of changes in the anterior chamber corner: coarse postoperative cicatrices and vitreo-corneal adhesions. Diabetic retinopathy was equally incident in all three groups of patients. Changes in the ciliary vessels can anticipate diabetic retinopathy. New vessels on ciliary body processes is an unfavorable prognostic sign as regards visual functions in patients with aphakia and increased intraocular pressure.