Tumor suppressive roles of eugenol in human lung cancer cells.

BACKGROUND: Eugenol, a natural compound available in Syzigium aromaticum (cloves), is exploited for various medicinal applications. Eugenol induces apoptosis and functions as an anti-cancer drug in several types of tumors. We investigated the tumor suppressive role and potential mechanisms of eugenol in human lung cancer cells. METHODS: Human embryonic lung fibroblast MRC-5 and lung cancer adenocarcinoma A549 cells were incubated with or without various concentrations of eugenol for 24 hours. Cell counting kit 8 and crystal violet staining assays were performed to detect cell viability. The cell migration and invasion abilities were also determined by wound healing and transwell assays. Finally, Western blotting assay was performed to examine the changes in lung cancer cell viability and invasion of downstream targets after treatment with eugenol. RESULTS: Eugenol could inhibit cell viability in lung cancer cells. Furthermore, eugenol obviously impaired cell migration and invasion. Finally, the expression levels of phosphate-Akt and MMP-2 in lung cancer cells were reduced after treatment with eugenol. CONCLUSION: Our results demonstrated the tumor suppressive roles of eugenol on lung cancer cell proliferation, migration, and invasion partially through the PI3K/Akt pathway and MMP activity in vitro. These results suggest eugenol as a potential chemotherapeutic agent against human lung cancer.

Effect of Enteral Nutrition Liquid with Different Dietary Fiber Content on Serum Protein in Patients with Stress Hyperglycemia / 医药导报

Objective To compare the effect of enteral nutrition liquid with different dietary fiber content on serum protein in patients with stress hyperglycemia.Methods A total of 90 patients with stress hyperglycemia were randomly divided into three groups:enteral nutritional suspension (SP) group (Peptisorb),enteral nutritional suspension (TPF-FOS) group (Jevety) and enteral nutritional suspension (TPF-D) group (Glucerna),each group consisting of 30 patients.In the three groups,prealbumi (PA),serum albumin (ALB),retinol binding protein (RBP),transfer-rin (TRF) and hemoglobin (Hb) were continuously measured on the 1st,2nd,3rd,4th,5th,6th and 7th day.Data were analyzed by SPSS 15.0.Variances on time and group were analyzed by repeated measures of general linear model.Results The PA,ALB,RBP,TRF and Hb were significantly different among Peptisorb group,Jevety group and Glucerna group (P＜ 0.05).The PA,ALB and Hb which were determined in different time,were not significantly impacted by Peptisorb,Jevety or Glucerna,and not significantly changed with time.The RBP and TRF which were determined at different time,were not impacted by Peptisorb,Jevety or Glucema,but time× group from RBP and TRF which were determined in different time,were significantly impacted in Peptisorb group,Jevety group and Glucerna group (P＜ 0.01).Conclusion The content from different dietary fiber significantly affects serum protein in patients with stress hyperglycemia,and TPF-D has the most significant effect on serum protein.

Alteration of the cerebral inter-hemispheric resting-state functional connectivity in amyotrophic lateral sclerosis：a voxel-mirrored homotopic connectivity MRI study / 中华放射学杂志

Objective To investigate the difference of cerebral inter-hemispheric functional connectivity between the patients with amyotrophic lateral sclerosis ( ALS) and healthy controls ( HC) by a newly developed voxel-mirrored homotopic connectivity ( VMHC) method based on resting-state functional MRI ( rs-fMRI) , as well as its correlation to the structural imaging indices and clinical neurological scales .Methods Twenty patients with ALS ( ALS group ) and 20 age-, and sex-matched healthy controls ( HC group) were examined by rs-fMRI and diffusion tensor imaging ( DTI ) on 3.0 T MR imaging system ( Siemens Trio Tim ).The rs-fMRI data preprocessing were performed using data processing assistant for rs-fMRI ( DPARSF) based on Matlab 2009a, and the rs-fMRI data analysis toolkit ( REST) with an automated VMHC approach was utilized to calculate and compare the VMHC correlation coefficients between the ALS group and the HC group[P0.05).Conclusions Abnormal VMHC coefficients were detected in ALS patients.The decreased VMHC in ACC and M1 are associated with the reduced micro-structure integrity of CC, meanwhile, and they may be related to disease severity.

Anti-oxidative and mitochondria protective effects of resveratrol on focal cerebral ischemia-reperfusion injury in rats / 中成药

AIM: To investigate anti-oxidative and mitochondria protective effects of resveratrol on focal cerebral ischemia-reperfusion injury in rats. METHODS: Middle cerebral artery occlusion(MACO) was used to induce focal cerebral ischemia-reperfusion model.After 24 h reperfusion,MDA,GSH,NO,LD content and SOD activity in brain homogenate were determined,MDA,GSH content and SOD,ATPase activity in mitochondria were also determined. RESULTS: Resveratrol significantly inhibited the increase in MDA,NO,LD content and the decrease in SOD activity and GSH content in brain after cerbral ischemia-reperfusion.Resveratrol also significantly inhibited the increase in MDA and the decrease in activity of SOD,Na~+-K~+-ATPase,Ca~(2+)-Mg~(2+)-ATPase in mitochondria. CONCLUSION: Resvertrol has protective effects on focal cerebral ischemia-reperfusion injury via attenuating cerebral oxygen free radical lipid peroxidation and protecting mitochondria.