Comparison of polyglactin-910 and polydioxanone for closure of the linea alba following caudal ventral midline laparotomy in sheep.

This study compared incisional complications after ventral midline laparotomy using 2 absorbable suture materials for apposition of the linea alba in sheep. The linea alba of 93 yearling sheep was sutured by 3 veterinarians in a simple continuous pattern using either polyglactin 910 (PG910; group PG) or polydioxanone (PDS; group PD). A blinded observer assessed surgical sites at the time of suture removal. Multivariate logistic regression was used to assess the association between incisional complications and variables (suture material used, veterinarian, skin suture removal time). The odds of incisional complications did not vary significantly with the type of suture material used (P = 0.11), veterinarian (P = 0.61) or skin suture removal time (P = 0.36). Most incisional complications were cutaneous suture sinus formation. Either PG910 or PDS may be used for linea alba closure in sheep.

Comparison de l'utilisation du polyglactin 910 et du polydioxanone pour suturer la ligne blanche lors de laparotomies ventrales médianes caudales chez la brebis. Cette étude compare les complications incisionnelles suite à une laparotomie ventrale médiane en utilisant 2 fils de sutures pour l'apposition de la ligne blanche chez la brebis. La ligne blanche de 93 brebis a été suturée par 3 vétérinaires en points simples continus avec du polyglactin 910 (PG910; groupe PG) ou du polydioxanone (PDS; groupe PD). Une observation à l'aveugle des sites chirurgicaux a été effectuée lors du retrait des points de suture. Une régression logistique multivariée a été utilisée pour déterminer l'association entre les complications incisionnelles et les variables (matériel de suture utilisé, vétérinaire et temps de retrait des sutures cutanées). Les chances de complications ne variaient pas selon le type de matériel de suture utilisé (P = 0,11), le vétérinaire (P = 0,61) ou la période de retrait des points cutanés (P = 0,36). La majorité des complications étaient des fistules associées aux sutures cutanées. Le PG910 ou le PDS peut être utilisé pour l'apposition de la ligne blanche chez la brebis.(Traduit par les auteurs).

Follicular expression of follicle stimulating hormone receptor variants in the ewe.

BACKGROUND: Several alternatively-spliced mRNA transcripts of the follicle stimulating hormone receptor (FSHR) have been identified in sheep, including FSHR-1 (G protein-coupled form), FSHR-2 (dominant negative form), and FSHR-3 (growth factor type-1 form). Our objective was to determine which of these variants is predominantly expressed in follicles collected from ewes at various times after estrus. METHODS: Suffolk-cross ewes (n = 8) were allowed to come into estrus naturally and were euthanized 24 (n = 3), 36 (n = 3), or 48 (n = 2) hours after the onset of estrus. All visible follicles were measured, aspirated and pooled according to follicular diameter: small (<= 2.0 mm), medium (2.1-4.0 mm), large (4.1-6.0 mm), and preovulatory (> = 6.1 mm). Aspirated cells were separated from follicular fluid by centrifugation. Total RNA was extracted from cell pellets and reverse transcribed. The resulting cDNA was subjected to qPCR, using primer sets designed to amplify each variant specifically. Gene expression was normalized to that of beta-actin within samples, and compared by analysis of variance with the level of significant differences set at p < .05. RESULTS: Relative expression of FSHR-3 exceeded that of both FSHR-1 and FSHR-2 in medium follicles, and tended to be higher in small follicles (p = .09) regardless of time after onset of estrus, and thus results from different time points were pooled. Expression of FSHR-3 was greater than that of FSHR-2 and luteinizing hormone receptor (LHR) in small and medium follicles. Expression of LHR was greatest in preovulatory follicles. CONCLUSIONS: These experiments show that in addition to the well characterized G protein-coupled form of the FSHR, alternatively spliced variants of the FSHR may participate in follicular dynamics during follicular waves of the sheep estrous cycle. Furthermore, these results indicate that an "alternatively" spliced form of the FSHR (FSHR-3) is the predominant form of the FSHR in the sheep.