RESUMO
Here, we prepared a magnetic nanocomposite system composed of a cluster of magnetite nanoparticles coated with silica shell (MSNPs) with an average diameter of 140 ± 20 nm and conjugated with CD9 antibody (AntiCD9) using different strategies including adsorption or chemical conjugation of antibody molecules to either aminated MSNPs (AMSNPs) or carboxylated MSNPs (CMSNPs). Then, MSNPs were employed to isolate exosomes from ultracentrifuge-enriched solution, PC3 cell-culture medium, or exosome-spiked simulated plasma samples. Quantitative tests using nanoparticle-tracking analysis confirmed antibody-covalently conjugated MSNPs, i.e. the AntiCD9-AMSNPs and AntiCD9-CMSNPs enabled >90% recovery of exosomes. Additionally, the exosomes isolated with AntiCD9-CMSNPs showed higher recovery efficiency compared to the AntiCD9-AMSNPs. For both nanoadsorbents, lower protein impurities amounts were obtained as compared to that of exosomes isolated by ultracentrifugation and Exocib kit. The mean diameter assessment of the isolated exosomes indicates that particles isolated by using AntiCD9-AMSNPs and AntiCD9-CMSNPs have smaller sizes (136 ± 2.64 nm and 113 ± 11.53 nm, respectively) than those obtained by UC-enriched exosomes (140.9 ± 1.6 nm) and Exocib kit (167 ± 10.53 nm). Such promising results obtained in the isolation of exosomes recommend magnetic nanocomposite as an efficient tool for the simple and fast isolation of exosomes for diagnosis applications.
Assuntos
Exossomos , Anticorpos/metabolismo , Exossomos/química , Fenômenos Magnéticos , Proteínas/análise , Ultracentrifugação/métodos , Nanocompostos/químicaRESUMO
Exosomes as cell-derived vesicles are promising biomarkers for noninvasive and early detection of different types of cancer. However, a straightforward and cost-effective technique for isolation of exosomes in routine clinical settings is still challenging. Herein, we present for the first time, a novel coaxial nanofiber structure for the exosome isolation from body fluids with high efficiency. Coaxial nanofiber structure is composed of polycaprolactone polymer as core and a thin layer of gelatin (below 10 nm) as the shell. The thermo-sensitive thin layer of gelatin can efficiently release the captured exosome by specific antibody namely, CD63, whenever its temperature raised to the physiological temperature of 37 °C. Moreover, the thin layer of gelatin induces less contamination to separated exosomes. The interconnected micro-pores of electrospun nanofibrous membrane insurances large surface area for immobilization of specific antibody for efficient exosome capturing. The efficacy of exosome isolation is determined by direct ELISA and compared with ultracentrifugation technique. For the exosome isolation, it was observed that over 87% of exosomes existed in the culture medium can be effectively isolated by coaxial electrospun nanofibers with the average thickness of 50 µm. Therefore, this promising technique can be substituted for the traditional techniques for exosome isolation which are mostly suffering from low efficacy, high cost, and troublesome process.