Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 17 de 17
Filtrar
1.
Eur J Appl Physiol ; 118(2): 225-238, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29071380

RESUMO

PURPOSE: Sarcopenia can begin from the 4-5th decade of life and is exacerbated by obesity and inactivity. A combination of resistance exercise (RE) and endurance exercise is recommended to combat rising obesity and inactivity levels. However, work continues to elucidate whether interference in adaptive outcomes occur when RE and endurance exercise are performed concurrently. This study examined whether a single bout of concurrent RE and high-intensity interval training (HIIT) alters the satellite cell response following exercise compared to RE alone. METHODS: Eight sedentary, overweight/obese, middle-aged individuals performed RE only (8 × 8 leg extensions at 70% 1RM), or RE + HIIT (10 × 1 min at 90% HRmax on a cycle ergometer). Muscle biopsies were collected from the vastus lateralis before and 96 h after the RE component to determine muscle fiber type-specific total (Pax7+ cells) and active (MyoD+ cells) satellite cell number using immunofluorescence microscopy. RESULTS: Type-I-specific Pax7+ (P = 0.001) cell number increased after both exercise trials. Type-I-specific MyoD+ (P = 0.001) cell number increased after RE only. However, an elevated baseline value in RE + HIIT compared to RE (P = 0.046) was observed, with no differences between exercise trials at 96 h (P = 0.21). Type-II-specific Pax7+ and MyoD+ cell number remained unchanged after both exercise trials (all P ≥ 0.13). CONCLUSION: Combining a HIIT session after a single bout of RE does not interfere with the increase in type-I-specific total, and possibly active, satellite cell number, compared to RE only. Concurrent RE + HIIT may offer a time-efficient way to maximise the physiological benefits from a single bout of exercise in sedentary, overweight/obese, middle-aged individuals.


Assuntos
Treinamento Intervalado de Alta Intensidade/métodos , Obesidade/terapia , Treinamento Resistido/métodos , Sarcopenia/terapia , Células Satélites de Músculo Esquelético/patologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Proteína MyoD/metabolismo , Obesidade/complicações , Fator de Transcrição PAX7/metabolismo , Sarcopenia/etiologia , Células Satélites de Músculo Esquelético/metabolismo
2.
J Sports Sci ; 35(17): 1752-1758, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-28282757

RESUMO

Plasma heat shock protein 70 (HSP70) concentrations rise during heat stress, which can independently induce cytokine production. Upper body exercise normally results in modest body temperature elevations. The aim of this study was to investigate the impacts of additional clothing on the body temperature, cytokine and HSP70 responses during this exercise modality. Thirteen males performed 45-min constant-load arm cranking at 63% maximum aerobic power (62 ± 7%V̇O2peak) in either a non-permeable whole-body suit (intervention, INT) or shorts and T-shirt (control, CON). Exercise resulted in a significant increase of IL-6 and IL-1ra plasma concentrations (P < 0.001), with no difference between conditions (P > 0.19). The increase in HSP70 from pre to post was only significant for INT (0.12 ± 0.11ng∙mL-1, P < 0.01 vs. 0.04 ± 0.18 ng∙mL-1, P = 0.77). Immediately following exercise, Tcore was elevated by 0.46 ± 0.29 (INT) and 0.37 ± 0.23ºC (CON), respectively (P < 0.01), with no difference between conditions (P = 0.16). The rise in mean Tskin (2.88 ± 0.50 and 0.30 ± 0.89ºC, respectively) and maximum heat storage (3.24 ± 1.08 and 1.20 ± 1.04 J∙g-1, respectively) was higher during INT (P < 0.01). Despite large differences in heat storage between conditions, the HSP70 elevations during INT, even though significant, were very modest. Possibly, the Tcore elevations were too low to induce a more pronounced HSP70 response to ultimately affect cytokine production.


Assuntos
Braço/fisiologia , Vestuário , Citocinas/sangue , Exercício Físico/fisiologia , Proteínas de Choque Térmico HSP70/sangue , Resposta ao Choque Térmico/fisiologia , Regulação da Temperatura Corporal/fisiologia , Frequência Cardíaca/fisiologia , Humanos , Proteína Antagonista do Receptor de Interleucina 1/sangue , Interleucina-6/sangue , Masculino , Consumo de Oxigênio/fisiologia , Troca Gasosa Pulmonar/fisiologia , Adulto Jovem
3.
Eur J Appl Physiol ; 116(2): 395-404, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26590591

RESUMO

PURPOSE: Passive muscle heating has been shown to reduce the drop in post-warm-up muscle temperature (Tm) by about 25% over 30 min, with concomitant sprint/power performance improvements. We sought to determine the role of leg blood flow in this cooling and whether optimising the heating procedure would further benefit post-warm-up T m maintenance. METHODS: Ten male cyclists completed 15-min sprint-based warm-up followed by 30 min recovery. Vastus lateralis Tm (Tmvl) was measured at deep-, mid- and superficial-depths before and after the warm-up, and after the recovery period (POST-REC). During the recovery period, participants wore water-perfused trousers heated to 43 °C (WPT43) with either whole leg heating (WHOLE) or upper leg heating (UPPER), which was compared to heating with electrically heated trousers at 40 °C (ELEC40) and a non-heated control (CON). The blood flow cooling effect on Tmvl was studied comparing one leg with (BF) and without (NBF) blood flow. RESULTS: Warm-up exercise significantly increased Tmvl by ~3 °C at all depths. After the recovery period, BF Tmvl was lower (~0.3 °C) than NBF Tmvl at all measured depths, with no difference between WHOLE versus UPPER. WPT43 reduced the post-warm-up drop in deep-Tmvl (-0.12 °C ± 0.3 °C) compared to ELEC40 (-1.08 ± 0.4 °C) and CON (-1.3 ± 0.3 °C), whereas mid- and superficial-Tmvl even increased by 0.15 ± 0.3 and 1.1 ± 1.1 °C, respectively. CONCLUSION: Thigh blood flow contributes to the post-warm-up Tmvl decline. Optimising the external heating procedure and increasing heating temperature of only 3 °C successfully maintained and even increased T mvl, demonstrating that heating temperature is the major determinant of post-warm-up Tmvl cooling in this application.


Assuntos
Regulação da Temperatura Corporal , Músculo Esquelético/fisiologia , Fluxo Sanguíneo Regional , Exercício de Aquecimento , Temperatura Corporal , Temperatura Alta , Humanos , Perna (Membro)/irrigação sanguínea , Perna (Membro)/fisiologia , Masculino , Músculo Esquelético/irrigação sanguínea , Adulto Jovem
4.
Eur J Appl Physiol ; 114(6): 1207-16, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-24577845

RESUMO

PURPOSE: This study aimed to quantify the relationship between venous and capillary blood sampling methods for the measurement of plasma interleukin-6 (IL-6). A parallel study was conducted to determine the possibility of measuring IL-6 in sweat using an enzyme-linked immunosorbent assay (ELISA) and investigate the relationship between plasma- and sweat-derived measures of IL-6. METHODS: Twelve male participants were recruited for the measurement of IL-6 at rest and during exercise (study 1). An additional group of five female participants was recruited for the measurement of IL-6 in venous blood versus sweat at rest and following exercise (study 2). In study 1, venous and capillary blood samples were collected at rest and in response to exercise. In study 2, venous and sweat samples were collected following exercise. RESULTS: Mean plasma IL-6 concentration was not different between venous and capillary blood sampling methods either at rest (4.27 ± 5.40 vs. 4.14 ± 4.45 pg ml(-1)), during (5.40 ± 5.17 vs. 5.58 ± 6.34 pg ml(-1)), or in response to exercise (6.95 ± 6.37 vs. 6.99 ± 6.74 pg ml(-1)). There was no IL-6 detectable in sweat either at rest or following exercise. CONCLUSION: There are no differences in the measurement of plasma IL-6 using either venous or capillary blood sampling methods. Capillary measurement represents a minimally invasive way of measuring IL-6 and detecting changes in IL-6, which are linked to fatigue and overtraining.


Assuntos
Coleta de Amostras Sanguíneas/métodos , Exercício Físico , Interleucina-6/sangue , Adulto , Análise Química do Sangue/métodos , Capilares , Feminino , Humanos , Interleucina-6/análise , Masculino , Descanso , Suor/química , Veias
5.
Eur J Appl Physiol ; 113(11): 2713-21, 2013 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-23974847

RESUMO

PURPOSE: Having previously shown the use of passive external heating between warm-up completion and sprint cycling to have had a positive effect on muscle temperature (T m) and maximal sprint performance, we sought to determine whether adding passive heating during active warm up was of further benefit. METHODS: Ten trained male cyclists completed a standardised 15 min sprint based warm-up on a cycle ergometer, followed by 30 min passive recovery before completing a 30 s maximal sprint test. Warm up was completed either with or without additional external passive heating. During recovery, external passive leg heating was used in both standard warm-up (CONHOT) and heated warm-up (HOTHOT) conditions, for control, a standard tracksuit was worn (CON). RESULTS: T m declined exponentially during CON, CONHOT and HOTHOT reduced the exponential decline during recovery. Peak (11.1 %, 1561 ± 258 W and 1542 ± 223 W), relative (10.6 % 21.0 ± 2.2 W kg(-1) and 20.9 ± 1.8 W kg(-1)) and mean (4.1 %, 734 ± 126 W and 729 ± 125 W) power were all improved with CONHOT and HOTHOT, respectively compared to CON (1,397 ± 239 W; 18.9 ± 3.0 W kg(-1) and 701 ± 109 W). There was no additional benefit of HOTHOT on T m or sprint performance compared to CONHOT. CONCLUSION: External heating during an active warm up does not provide additional physiological or performance benefit. As noted previously, external heating is capable of reducing the rate of decline in T m after an active warm-up, improving subsequent sprint cycling performance.


Assuntos
Desempenho Atlético/fisiologia , Regulação da Temperatura Corporal , Músculo Esquelético/fisiologia , Exercício de Aquecimento/fisiologia , Adulto , Ciclismo/fisiologia , Estudos de Casos e Controles , Frequência Cardíaca , Calefação , Humanos , Masculino
6.
Cell Physiol Biochem ; 27(6): 625-36, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-21691080

RESUMO

BACKGROUND: Potential roles for undifferentiated skeletal muscle stem cells or satellite cells in muscle hypertrophy and repair have been reported, however, the capacity, the mode and the mechanisms underpinning migration have not been investigated. We hypothesised that damaged skeletal myoblasts would elicit a mesenchymal-like migratory response, which could be precisely tracked and subsequently manipulated. METHODS: We therefore established a model of mechanical damage and developed a MATLAB(TM) tool to measure the migratory capacity of myoblasts in a non-subjective manner. RESULTS: Basal migration following damage was highly directional, with total migration distances of 948µm ± 239µm being recorded (average 0-24 hour distances: 491µm ± 113µm and 24-48 hour distances: 460µm ± 218µm). Pharmacological inhibition of MEK or PI3-K using PD98059 (20µM) or LY294002 (5µm), resulted in significant reduction of overall cell migration distances of 38% (p<0.001) and 39.5% (p<0.0004), respectively. Using the semi-automated cell tracking using MATLAB(TM) program we validated that not only was migration distance reduced as a consequence of reduced cell velocity, but critically also as a result of altered directionality of migration. CONCLUSION: These studies demonstrate that murine myoblasts in culture migrate and provide a good model for studying responsiveness to damage in vitro. They illustrate for the first time the powerful tool that MATLAB(TM) provides in determining that both velocity and directional capacity influence the migratory potential of cellular movement with obvious implications for homing and for metastases.


Assuntos
Automação , Movimento Celular , Mioblastos/citologia , Animais , Linhagem Celular , Meios de Cultura , Camundongos , Músculo Esquelético/citologia , Músculo Esquelético/efeitos dos fármacos
7.
Int J Sports Physiol Perform ; 16(1): 51-58, 2021 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-33120359

RESUMO

PURPOSE: Cycling time trials (TTs) are characterized by riders' adopting aerodynamic positions to lessen the impact of aerodynamic drag on velocity. The optimal performance requirements for TTs likely exist on a continuum of rider aerodynamics versus physiological optimization, yet there is little empirical evidence to inform riders and coaches. The aim of the present study was to investigate the relationship between aerodynamic optimization, energy expenditure, heat production, and performance. METHODS: Eleven trained cyclists completed 5 submaximal exercise tests followed by a TT. Trials were completed at hip angles of 12° (more horizontal), 16°, 20°, 24° (more vertical), and their self-selected control position. RESULTS: The largest decrease in power output at anaerobic threshold compared with control occurred at 12° (-16 [20] W, P = .03; effect size [ES] = 0.8). There was a linear relationship between upper-body position and heat production (R2 = .414, P = .04) but no change in mean body temperature, suggesting that, as upper-body position and hip angle increase, convective and evaporative cooling also rise. The highest aerodynamic-physiological economy occurred at 12° (384 [53] W·CdA-1·L-1·min-1, ES = 0.4), and the lowest occurred at 24° (338 [28] W·CdA-1·L-1·min-1, ES = 0.7), versus control (367 [41] W·CdA-1·L-1·min-1). CONCLUSION: These data suggest that the physiological cost of reducing hip angle is outweighed by the aerodynamic benefit and that riders should favor aerodynamic optimization for shorter TT events. The impact on thermoregulation and performance in the field requires further investigation.


Assuntos
Desempenho Atlético , Ciclismo/fisiologia , Regulação da Temperatura Corporal , Postura , Tronco , Atletas , Metabolismo Energético , Teste de Esforço , Humanos
8.
Int J Sports Physiol Perform ; 16(11): 1573-1579, 2021 11 01.
Artigo em Inglês | MEDLINE | ID: mdl-33831843

RESUMO

PURPOSE: To examine whether an ecologically valid, intermittent, sprint-based warm-up strategy impacted the ergogenic capacity of individualized sodium bicarbonate (NaHCO3) ingestion on 4-km cycling time-trial (TT) performance. METHODS: A total of 8 male cyclists attended 6 laboratory visits for familiarization, determination of time to peak blood bicarbonate, and 4 × 4-km cycling TTs. Experimental beverages were administered doubleblind. Treatments were conducted in a block-randomized, crossover order: intermittent warm-up + NaHCO3 (IWSB), intermittent warm-up + placebo, control warm-up + NaHCO3 (CWSB), and control warm-up + placebo (CWP). The intermittent warm-up comprised exercise corresponding to lactate threshold (5 min at 50%, 2 min at 60%, 2 min at 80%, 1 min at 100%, and 2 min at 50%) and 3 × 10-second maximal sprints. The control warm-up comprised 16.5 minutes cycling at 150 W. Participants ingested 0.3 g·kg body mass-1 NaHCO3 or 0.03 g·kg body mass-1 sodium chloride (placebo) in 5 mL·kg body mass-1 fluid (3:2, water and sugar-free orange squash). Paired t tests were conducted for TT performance. Hematological data (blood bicarbonate and blood lactate) and gastrointestinal discomfort were analyzed using repeated-measures analysis of variance. RESULTS: Performance was faster for CWSB versus IWSB (5.0 [6.1] s; P = .052) and CWP (5.8 [6.0] s; P = .03). Pre-TT bicarbonate concentration was elevated for CWSB versus IWSB (+9.3 mmol·L-1; P < .001) and CWP (+7.1 mmol·L-1; P < .001). Post-TT blood lactate concentration was elevated for CWSB versus CWP (+2.52 mmol·L-1; P = .022). Belching was exacerbated pre-warm-up for IWSB versus intermittent warm-up +placebo (P = .046) and CWP (P = .027). CONCLUSION: An intermittent, sprint-based warm-up mitigated the ergogenic benefits of NaHCO3 ingestion on 4-km cycling TT performance.


Assuntos
Desempenho Atlético , Substâncias para Melhoria do Desempenho , Exercício de Aquecimento , Ciclismo , Método Duplo-Cego , Ingestão de Alimentos , Humanos , Concentração de Íons de Hidrogênio , Masculino , Bicarbonato de Sódio
9.
Appl Physiol Nutr Metab ; 46(11): 1345-1353, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34062089

RESUMO

Eight highly trained middle-distance runners (1500 m personal best 4:01.4 ± 0:09.2 min) completed two 7-day tapers, separated by at least 3 weeks of regular training: (i) prescribed using prediction models from elite middle-distance runners, where continuous running volume was reduced by 30% and interval intensity was equal to 1500 m race pace (RP); and (ii) continuous running volume was reduced by 60% and intensity of the final interval session was completed at 110% of 1500 m race pace (HI). Performance was assessed using 1500 m time trials on an indoor 200 m track 1 day before, and 1 day after each taper. Performance time was improved after HI by 5.2 ± 3.7 s (mean ± 90% confidence limits, p = 0.03) and by 3.2 ± 3.8 s after RP (p = 0.15). The first and second 300 m segments of the 1500 m time trial were faster post-taper in RP (p = 0.012 and p = 0.017, respectively) and HI (both p = 0.012). Running faster than race pace late in a low-volume taper is recommended to improve 1500 m track performance. A positive pacing strategy is adopted after tapering, although care should be taken to avoid an over-fast start. Novelty: A large reduction in volume during tapering and an increase in final interval session intensity improves running performance. Athletes adopt a negative pacing strategy before tapering and a positive-pacing strategy after tapering.


Assuntos
Desempenho Atlético/fisiologia , Condicionamento Físico Humano/métodos , Corrida/fisiologia , Comportamento Competitivo/fisiologia , Estudos Cross-Over , Humanos , Masculino , Fatores de Tempo , Adulto Jovem
10.
Int J Sports Physiol Perform ; 14(3): 323-330, 2019 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-30160552

RESUMO

PURPOSE: Cycling time-trial performance can be compromised by moderate to high ambient temperatures. It has become commonplace to implement precooling prior to competition to alleviate this performance decline. However, little is known about the ambient temperature threshold above which precooling becomes an effective strategy for enhancing endurance performance. The aim of this study was to investigate the effect of precooling in different environmental temperatures on time-trial (TT) performance. METHODS: Trained cyclists completed 2 TTs with (COLD) and without (CON) precooling using an ensemble of  ice vest and sleeves in ambient temperatures of 24°C, 27°C, and 35°C. RESULTS: TT performance was faster following COLD in both 35°C (6.2%) and 27°C (2.6%; both Ps < .05) but not 24°C (1.2%). Magnitude-based inferential statistics indicate that COLD was very likely beneficial to performance in 35°C, likely beneficial in 27°C, and possibly beneficial in 24°C. Mean power was 2.4%, 2.5%, and 5.6% higher following COLD and considered to be likely beneficial in 24°C and very likely beneficial in 27°C and 35°C. COLD reduced mean skin temperature throughout the warm-up and into the TT in all ambient temperatures (P < .05). Sweat loss was lower following COLD in 24°C and 27°C but not 35°C. There was no effect of COLD on gastrointestinal temperature at any point. CONCLUSIONS: Precooling with an ice vest and sleeves is likely to have a positive effect on TT performance at temperatures above 24°C, with a clear relationship between ambient temperature and the magnitude of effect of precooling.


Assuntos
Ciclismo/fisiologia , Regulação da Temperatura Corporal , Vestuário , Temperatura Alta , Gelo , Resistência Física/fisiologia , Crioterapia/métodos , Trato Gastrointestinal/fisiologia , Frequência Cardíaca , Humanos , Masculino , Percepção , Temperatura Cutânea , Exercício de Aquecimento , Adulto Jovem
SELEÇÃO DE REFERÊNCIAS
Detalhe da pesquisa