Evaluation of the Anti-Cancer Potential of Rosa damascena Mill. Callus Extracts against the Human Colorectal Adenocarcinoma Cell Line.

Chemotherapy is an aggressive form of chemical drug therapy aiming to destroy cancer cells. Adjuvant therapy may reduce hazards of chemotherapy and help in destroying these cells when obtained from natural products, such as medical plants. In this study, the potential therapeutic effect of Rosa damascena callus crude extract produced in vitamin-enhanced media is investigated on colorectal cancer cell line Caco-2. Two elicitors, i.e., L-ascorbic acid and citric acid at a concentration of 0.5 g/L were added to the callus induction medium. Callus extraction and the GC-MS analysis of methanolic crude extracts were also determined. Cytotoxicity, clonogenicity, proliferation and migration of Caco-2 colorectal cancer cells were investigated using MTT cytotoxicity, colony-forming, Ki-67 flow cytometry proliferation and Migration Scratch assays, respectively. Our results indicated that L-ascorbic acid treatment enhanced callus growth parameters and improved secondary metabolite contents. It showed the least IC50 value of 137 ug/mL compared to 237 ug/mL and 180 ug/mL in the citric acid-treated and control group. We can conclude that R. damascena callus elicited by L-ascorbic acid improved growth and secondary metabolite contents as well as having an efficient antiproliferative, anti-clonogenic and anti-migratory effect on Caco-2 cancer cells, thus, can be used as an adjuvant anti-cancer therapy.

Use of algal biorefinery waste and waste office paper in the development of xerogels: A low cost and eco-friendly biosorbent for the effective removal of congo red and Fe (II) from aqueous solutions.

The present study investigated the use of algae biorefinery waste and wastepaper in the preparation of cost-effective and eco-friendly xerogels for the removal of congo red (CR) and Fe2+. The xerogel properties such as density, swelling degree and porosity were modified by incorporating alginate extracted from the brown seaweed Cystoseira trinodis. The developed biosorbents exhibited a light and porous network structure and were characterized by a fast uptake of CR and Fe2+ and adsorption efficiency was increased at pH 6-8. The equilibrium adsorption capacity was found to be 6.20-7.28 mg CR g-1 biosorbent and 8.08-8.39 mg Fe2+ g-1 biosorbent using different xerogels. The adsorption of CR obeyed first-order kinetics, while, Fe2+ followed second-order kinetics. Intraparticle diffusion model suggested a boundary layer effect. The adsorption capacity was maximally obtained as 41.15 mg g-1 and 169.49 mg g-1 for CR and Fe2+ using wastepaper/Spirulina and wastepaper/alginate/Spirulina xerogel, respectively. Temkin isotherm fitted better to the equilibrium data of CR adsorption than Langmuir and Freundlich models. While, equilibrium data of Fe2+ exhibited a best fit to both Langmuir and Freundlich models. Additionally, the Dubinin-Radushkevich isotherm suggested that adsorption mechanism of CR or Fe2+ is predominately physisorption. Investigation of thermodynamic parameters such as ΔH° and ΔS° and ΔG° confirmed the feasibility, spontaneity, randomness and endothermic nature of the adsorption process. Electrostatic attraction, H-bonding and n-π interactions were mainly involved in the biosorption process of CR. The results of this study showed that the developed xerogels could be effectively applied for dye and heavy metal removal at low concentrations.

Bioremoval of heavy metals and nutrients from sewage plant by Anabaena oryzae and Cyanosarcina fontana.

The present study demonstrated the growth of two species of cyanobacteria on wastewater isolated from sewage plant in Aswan, Egypt. We evaluated their efficiency for eliminating nitrogen, phosphorus, chemical oxygen demand (COD) and heavy metals (Fe(2+), Pb(2+), Cu(2+), and Mn(2+)). The growth of Cyanosarcina fontana has supported wastewater as a growth medium than Anabaena oryzae compared to standard medium. The nutrients concentration such as COD, NO3-N and PO4-P were decreased by the growth of A. oryzae and C. fontana in the wastewater after primary settling and centrate. However, the reduction of COD was less efficient than the other nutrients. The reduction percentage of COD, NO3-N and PO4-P reached 39.3, 84.1 and 90.7% as well as 54.6, 83.1, and 89.8%, in cultures of A. oryzae and C. fontana grown in the wastewater after primary settling, respectively. The reduction amounted to 10.1, 76.8, and 63.0% by A. oryzae and 43.2, 62.1, and 74.8% by C. fontana, grown in the centrate, respectively. Cyanobacteria species have the ability to accumulate the heavy metals from the wastewater to level far than the exceeding metal level in the water. Whereas, the heavy metals biosorption performance of C. fontana was higher in accumulating Fe(2+) (93.95%), Pb(2+) (81.21%), Cu(2+) (63.9%), and Mn(2+) (48.49%) compared to A. oryzae. The biosorption ability is dependent on the nature of the adsorbent studied and the type of wastewater treated. Therefore, removal of heavy metals and nutrients by the tested algae is strongly recommended as a powerful technique for the removal of pollutants from wastewater.

Hormesis effects of phenol on growth and cellular metabolites of Chlorella sp. under different nutritional conditions using response surface methodology.

The present study investigated the effects of different phenol concentrations (200 - 1000 mg L-1) towards Chlorella sp. under different culture conditions (light vs. dark) and NaNO3 concentrations (0 - 0.1 g L-1) using central composite design. Phenol induced hormesis effects on the algal growth and cellular metabolites. Nitrate was identified as a crucial factor for promoting the uptake of phenol by Chlorella cells, while light was a limiting factor for growth, but the phyco-toxicity of phenol was decreased in the dark. The pigment contents were generally increased in the treated cells to protect against the oxidative phenol stress. The incorporation of 200 mg L-1 phenol and 0.05 g L-1 NaNO3 to the illuminated cells markedly promoted biomass and lipid contents to 0.22 g L-1 and 26.26% w/w, which was 44 and 112% higher than the phenol-less control, respectively. Under the same conditions, the increase of phenol concentration to 600 mg L-1, the protein contents were increased to 18.59% w/w. Conversely, the algal cells were able to accumulate more than 60% w/w of soluble carbohydrates under dark conditions at 600 mg L-1 of phenol. Nitrate replete conditions stimulated lipid accumulation at the expense of protein biosynthesis. Furthermore, most of the treatments showed an increase of H2O2 and malonaldehyde contents, especially for the illuminated cells. However, catalase activity tended to increase under dark conditions, especially at low phenol and nitrate concentrations. This study is valuable in indicating the effects of phenol on microalgae by exploiting response surface methodology, which can be applied as a powerful tool in growth monitoring and toxicity assessment.

Kinetic, Isotherm and Thermodynamic Aspects of Zn2+ Biosorption by Spirulina platensis: Optimization of Process Variables by Response Surface Methodology.

The aim of this study was to assess the efficiency of Spirulina platensis for removing Zn2+ ions from the aqueous solutions. The optimized conditions of 4.48 g/L algal dose, pH of 6.62 and initial zinc concentration of 29.72 mg/L obtained by response surface methodology were employed for Zn2+ biosorption by S. platensis and up to 97.90% Zn2+ was removed, showing that there is a favorable harmony between the experimental data and model predictions. Different kinetic and equilibrium models were used to characterize the biosorption manner of Spirulina as a biosorbent. The kinetic manner of Zn2+ biosorption was well characterized by the pseudo-second-order, implying that the adsorption process is chemical in nature. The Langmuir and Dubinin-Radushkevich isotherm models were best fit to the equilibrium data. The maximum adsorption capacity of the Langmuir monolayer was 50.7 mg/g. Furthermore, the thermodynamic analysis revealed that Zn2+ biosorption was endothermic, spontaneous and feasible. As a result of biosorption process, FTIR, SEM, and EDX investigations indicated noticeable alterations in the algal biomass's properties. Therefore, the dried Spirulina biomass has been shown to be cost-effective and efficient for removing the heavy metals, particularly zinc ions from wastewater, and the method is practicable, and environmentally acceptable.

Statistical optimization, kinetic, equilibrium isotherm and thermodynamic studies of copper biosorption onto Rosa damascena leaves as a low-cost biosorbent.

In this study, Rosa damascena leaf powder was evaluated as a biosorbent for the removal of copper from aqueous solutions. Process variables such as the biosorbent dose, pH, and initial copper concentration were optimized using response surface methodology. A quadratic model was established to relate the factors to the response based on the Box-Behnken design. Analysis of variance (ANOVA) was used to assess the experimental data, and multiple regression analysis was used to fit it to a second-order polynomial equation. A biosorbent dose of 4.0 g/L, pH of 5.5, and initial copper concentration of 55 mg/L were determined to be the best conditions for copper removal. The removal of Cu2+ ions was 88.7% under these optimal conditions, indicating that the experimental data and model predictions were in good agreement. The biosorption data were well fitted to the pseudo-second-order and Elovich kinetic models. The combination of film and intra-particle diffusion was found to influence Cu2+ biosorption. The Langmuir and Dubinin-Radushkevich isotherm models best fit the experimental data, showing a monolayer isotherm with a qmax value of 25.13 mg/g obtained under optimal conditions. The thermodynamic parameters showed the spontaneity, feasibility and endothermic nature of adsorption. Scanning electron microscopy, energy-dispersive X-ray spectroscopy, and Fourier transform infrared spectroscopy were used to characterize the biosorbent before and after Cu2+ biosorption, revealing its outstanding structural characteristics and high surface functional group availability. In addition, immobilized R. damascena leaves adsorbed 90.7% of the copper from aqueous solution, which is more than the amount adsorbed by the free biosorbent (85.3%). The main mechanism of interaction between R. damascena biomass and Cu2+ ions is controlled by both ion exchange and hydrogen bond formation. It can be concluded that R. damascena can be employed as a low-cost biosorbent to remove heavy metals from aqueous solutions.

Evaluation of the Phytochemical and Pharmacological Potential of Taif's Rose (Rosa damascena Mill var. trigintipetala) for Possible Recycling of Pruning Wastes.

This study investigated the phytochemical contents of Taif's rose pruning wastes and their potential application as phytomedicine, thereby practicing a waste-recycling perspective. In the Al-Shafa highland, four Taif rose farms of various ages were chosen for gathering the pruning wastes (leaves and stems) for phytochemical and pharmacological studies. The leaves and stems included significant amounts of carbohydrates, cardiac glycosides, alkaloids, flavonoids, and other phenolic compounds. The cardiac glycoside and flavonoid contents were higher in Taif rose stems, while the phenolic and alkaloid contents were higher in the plant leaves. Cardiovascular glycosides (2.98-5.69 mg g-1), phenolics (3.14-12.41 mg GAE g-1), flavonoids (5.09-9.33 mg RUE g -1), and alkaloids (3.22-10.96 mg AE g-1) were among the phytoconstituents found in rose tissues. According to the HPLC analysis of the phenolic compounds, Taif's rose contains flavonoid components such as luteolin, apigenin, quercetin, rutin, kaempferol, and chrysoeriol; phenolics such as ellagic acid, catechol, resorcinol, gallic acid, and phloroglucinol; alkaloids such as berbamine, jatrorrhizine, palmatine, reticuline, isocorydine, and boldine. Warm water extract was highly effective against Bacillus subtilis, Escherichia coli, and Proteus vulgaris, whereas methanol and cold water extracts were moderately effective against Aspergillus fumigatus and Candida albicans. The study's findings suggested that Taif's rose wastes could be used for varied medical purposes.

Process optimization and modeling of Cd2+ biosorption onto the free and immobilized Turbinaria ornata using Box-Behnken experimental design.

The release of effluents containing cadmium ions into aquatic ecosystems is hazardous to humans and marine organisms. In the current investigation, biosorption of Cd2+ ions from aqueous solutions by freely suspended and immobilized Turbinaria ornata biomasses was studied. Compared to free cells (94.34%), the maximum Cd2+ removal efficiency reached 98.65% for immobilized cells obtained via Box-Behnken design under optimized conditions comprising algal doses of 5.04 g L-1 and 4.96 g L-1, pH values of 5.06 and 6.84, and initial cadmium concentrations of 25.2 mg L-1 and 26.19 mg L-1, respectively. Langmuir, Freundlich, and Temkin isotherm models were suitably applied, providing the best suit of data for free and immobilized cells, but the Dubinin-Radushkevich model only matched the immobilized algal biomass. The maximum biosorption capacity of Cd2+ ions increased with the immobilized cells (29.6 mg g-1) compared to free cells (23.9 mg g-1). The Cd2+ biosorption data obtained for both biomasses followed pseudo-second-order and Elovich kinetic models. In addition, the biosorption process is controlled by film diffusion followed by intra-particle diffusion. Cd2+ biosorption onto the free and immobilized biomasses was spontaneous, feasible, and endothermic in nature, according to the determined thermodynamic parameters. The algal biomass was further examined via SEM/EDX and FTIR before and after Cd2+ biosorption. SEM/EDX analysis revealed Cd2+ ion binding onto the algal surface. Additionally, FTIR analysis confirmed the presence of numerous functional groups (hydroxyl, carboxyl, amine, phosphate, etc.) participating in Cd2+ biosorption. This study verified that immobilized algal biomasses constitute a cost-effective and favorable biosorbent material for heavy metal removal from ecosystems.

Chemical role of α-tocopherol in salt stress mitigation by improvement in morpho-physiological attributes of sunflower (Helianthus annuus L.).

Elevated concentrations of salts in soil and water represent abiotic stresses. It considerably restricts plant productivity. However, the use of alpha-tocopherol (α-toc) as foliar can overcome this problem. It can improve crop productivity grown under salinity stress. Limited literature is documented regarding its optimum foliar application on sunflower. That's why the need for the time is to optimize α-toc foliar application rates for sunflower cultivated in salt-affected soil. A pot experiment was performed to select a better α-toc foliar application for mitigation of salt stress in different sunflower cultivars FH (572 and 621). There were 2 levels of salts, i.e., control (no salt stress) and sodium chloride (120 mM) and four α-toc foliar application (0, 100, 200, and 300 mg L-1). Results showed that foliar application of 100 mg/L- α-toc triggered the remarkable increase in fresh shoot weight, fresh root weight, shoot, and root lengths under salinity stress in FH-572 and FH-621 over 0 mg/L- α-toc. Foliar application of 200 mg/L- α-toc was most effective for improvement in chlorophyll a, chlorophyll b, total chlorophyll and carotenoids compared to 0 mg/L- α-toc. Furthermore, an increase in A was noted in FH-572 (17%) and FH-621 (22%) with α-toc (300 mg L-1) application under saline condition. In conclusion, the 100 and 200 mg/L- α-toc are the best application rates for the improvement in sunflower FH-572 and FH-621 growth, chlorophyll contents and gas exchange attributes. Further investigations are needed to select a better foliar application rate between 100 and 200 mg/L- α-toc at the field level under the different agro-climatic zone and soil types.

The impact of abiotic factors on the growth and lipid accumulation of some green microalgae for sustainable biodiesel production.

Three species of freshwater planktonic green microalgae: Ankistrodesmus braunii, Ankistrodesmus falcatus, and Scenedesmus incrassatulus, were isolated from the Nile water in Upper Egypt. These microalgae were exposed to nutritional (nitrogen, phosphorus, and iron) limitations and salinity stress to study their effects on the algal growth and to elevate the lipid content within their cells. The results indicated that exposure to these conditions had a significant impact on the algal growth. The lipid content of the studied algae increased as a result of the salinity stress. The highest lipid content was recorded in A. braunii culture treated with 50 mM NaCl (34.4% of dry weight) and S. incrassatulus cultures treated with 100 mM NaCl (37.7% of dry weight) on the 6th day of cultivation, while the culture of A. falcatus treated with 100 mM NaCl recorded the maximum lipid content (53% of dry weight) on the 10th day of the experiment. The biodiesel quality parameters of the fatty acid methyl ester profile of S. incrassatulus appeared to be in agreement with the international criteria. S. incrassatulus could be regarded as a quite promising feedstock for the biodiesel production.

Upgrading the antioxidant properties of fucoidan and alginate from Cystoseira trinodis by fungal fermentation or enzymatic pretreatment of the seaweed biomass.

The seaweed Cystoseira trinodis was fermented by different fungi prior to extraction of fucoidan and alginate to enhance their antioxidative potential. All the investigated fungi were able to produce fucoidanase (1.05-3.41 U/ml) and alginate lyase (7.27-18.59 U/mL). Different fungal species induced a reduction in the molecular weight (MW) of fucoidan and alginate in comparison to the unfermented control. The MW of fucoidan reduced by 41-81.5%, while the MW of alginate was reduced by 28-75%, depending on the fungal species. Significant increases in the fucose and sulphate contents of fucoidan and mannuronic/guluronic acid ratio of alginate were induced by fungal fermentation. Fungal pretreatment enhanced the ferric reducing antioxidant power, total antioxidant capacity and hydroxyl radical scavenging activity of both fucoidan and alginate. Additionally, enzymatic pretreatment of the macroalgal biomass assisted in the recovery of fucoidan and alginate with low molecular weight and enhanced antioxidative potential.

Fatty Acid Characterization and Biodiesel Production by the Marine Microalga Asteromonas gracilis: Statistical Optimization of Medium for Biomass and Lipid Enhancement.

Lipid production is an important indicator for evaluating microalgal species for biodiesel production. In this study, a new green microalga was isolated from a salt lake in Egypt and identified as Asteromonas gracilis. The main parameters such as biomass productivity, lipid content, and lipid productivity were evaluated in A. gracilis, cultivated in nutrient-starved (nitrogen, phosphorous), and salinity stress as a one-factor-at-a-time method. These parameters in general did not vary significantly from the standard nutrient growth media when these factors were utilized separately. Hence, response surface methodology (RSM) was assessed to study the combinatorial effect of different concentrations of the abovementioned factor conditions and to maximize the biomass productivity, lipid content, and lipid productivity of A. gracilis by determining optimal concentrations. RSM optimized media, including 1.36 M NaCl, 1 g/L nitrogen, and 0.0 g/L phosphorus recorded maximum biomass productivity, lipid content, and lipid productivity (40.6 mg/L/day, 39.3%, and 15.9 mg/L/day, respectively) which agreed well with the predicted values (40.1 mg/L/day, 43.6%, and 14.6 mg/L/day, respectively). Fatty acid profile of A. gracilis was composed of C16:0, C16:1, C18:0, C18:3, C18:2, C18:1, and C20:5, and the properties of fuel were also in agreement with international standards. These results suggest that A. gracilis is a promising feedstock for biodiesel production.

Optimization of alginate alkaline extraction technology from Sargassum latifolium and its potential antioxidant and emulsifying properties.

Alginate was recovered from Sargassum latifolium biomass using different conditions of alkali treatment. Box-Behnken experimental design was evaluated to study the influence of alkali:alga ratio, temperature and time on alginate yield, and its molecular weight (MW) and mannuronic/guluronic acid ratio (M/G). The second-order polynomial equations were analyzed by appropriate statistical methods. Extraction temperature and time were the most important factors during alginate alkaline extraction. MW and M/G ratio played an important role in controlling the reducing power of alginate. Increasing pH of the alginate solutions enhanced its reducing capacity, while thermal treatment showed a negative effect. Additionally, alginate exhibited good emulsion stabilizing capacities with diverse hydrophobic compounds. Emulsifying activity was less sensitive to temperature, ionic strength and more stable at acidic pH.

Statistical Optimization of Culture Variables for Enhancing Agarase Production by Dendryphiella arenaria Utilizing Palisada perforata (Rhodophyta) and Enzymatic Saccharification of the Macroalgal Biomass.

Agarase is a promising biocatalyst for several industrial applications. Agarase production was evaluated by the marine fungus Dendryphiella arenaria utilizing Palisada perforata as a basal substrate in semi-solid state fermentation. Seaweed biomass, glucose, and sucrose were the most significant parameters affecting agarase production, and their levels were further optimized using Box-Behnken design. The maximum agarase activity was 7.69 U/mL. Agarase showed a degree of thermostability with half-life of 99 min at 40 °C, and declining to 44.72 min at 80 °C. Thermodynamics suggested an important process of protein aggregation during thermal inactivation. Additionally, the enzymatic saccharification of the seaweed biomass using crude agarase was optimized with respect to biomass particle size, solid/liquid ratio, and enzyme loadings. The amount of biosugars obtained after optimization was 26.15 ± 1.43 mg/g. To the best of our knowledge, this is the first report on optimization of agarase in D. arenaria.

Phycoremediation and adsorption isotherms of cadmium and copper ions by Merismopedia tenuissima and their effect on growth and metabolism.

The current study tends to investigate the removal of cadmium and copper ions by Merismopedia tenuissima, grown in different concentrations of cadmium and copper ions, as well to investigate their effects on growth and metabolism. Sorption isotherms of Langmuir and Freundlich were obtained for the quantitative description of cadmium and copper uptake by M. tenuissima. Langmuir model adequately to describe the data of biosorption for these metals. However, the Freundlich model could work well in case of Cu(2+) only. M. tenuissima appears to be more efficient for removing Cd(2+) ions than Cu(2+). However, the affinity constant of Cu(2+) on the biomass of M. tenuissima was higher than Cd(2+) indicating that M. tenuissima is more tolerant to Cd(2+) phytotoxicity than Cu(2+). FTIR analysis of algae with and without biosorption revealed the presence of carboxyl, amino, amide and hydroxyl groups, which were responsible for biosorption of Cd(+2) and Cu(+2) ions.