RESUMO
The international Covid19-NMR consortium aims at the comprehensive spectroscopic characterization of SARS-CoV-2 RNA elements and proteins and will provide NMR chemical shift assignments of the molecular components of this virus. The SARS-CoV-2 genome encodes approximately 30 different proteins. Four of these proteins are involved in forming the viral envelope or in the packaging of the RNA genome and are therefore called structural proteins. The other proteins fulfill a variety of functions during the viral life cycle and comprise the so-called non-structural proteins (nsps). Here, we report the near-complete NMR resonance assignment for the backbone chemical shifts of the non-structural protein 10 (nsp10). Nsp10 is part of the viral replication-transcription complex (RTC). It aids in synthesizing and modifying the genomic and subgenomic RNAs. Via its interaction with nsp14, it ensures transcriptional fidelity of the RNA-dependent RNA polymerase, and through its stimulation of the methyltransferase activity of nsp16, it aids in synthesizing the RNA cap structures which protect the viral RNAs from being recognized by the innate immune system. Both of these functions can be potentially targeted by drugs. Our data will aid in performing additional NMR-based characterizations, and provide a basis for the identification of possible small molecule ligands interfering with nsp10 exerting its essential role in viral replication.
Assuntos
Espectroscopia de Ressonância Magnética , SARS-CoV-2/química , Proteínas Virais Reguladoras e Acessórias/química , Motivos de Aminoácidos , Isótopos de Carbono , Exorribonucleases/química , Hidrogênio , Ligação de Hidrogênio , Ligantes , Metiltransferases , Isótopos de Nitrogênio , Estrutura Secundária de Proteína , RNA Viral , Envelope Viral , Proteínas não Estruturais Virais/química , Replicação Viral , Dedos de ZincoRESUMO
The payoff to successful time management is getting more out of your life and your career. This is accomplished by spending less time with your time robbers and more time on things that really have value to you. To gather data on how time management principles apply to clinical laboratory managers, in-depth surveys were conducted. These interviews produced some interesting findings about typical time robbers of clinical laboratory managers, as well as some innovative solutions. This article focuses on three principles of time management: Principle #1: Do Some Analysis of Your Use of Time. Principle #2: List Goals and Set Priorities. Principle #3: Plan Your Time Regularly.