RESUMO
Gout is a disorder that triggers a severe inflammatory reaction which generates episodes of intense pain and discomfort to the patient. Arctium minus (Hill) Bernh. (Asteraceae) is known as "burdock" and displays anti-inflammatory, antinociceptive, against rheumatic pain and radical-scavenging activities. Species of the genus Arctium have been used in assistant therapy of gout and other inflammatory processes. We investigated the antinociceptive and anti-edematogenic effects of the crude extract of A. minus seeds in an acute gout attack model induced by intra-articular injection of monosodium urate (MSU) crystals in adult male Swiss mice (25-30 g). The crude extract of A. minus (100 mg/kg, p.o.) reduced the mechanical allodynia induced by the injection of MSU (1.25 mg/site, i.a.) from 4 until 8 h after its administration. A. minus seeds crude extract prevented mechanical allodynia at doses of 30 and 100 mg/kg, but not 10 mg/kg. Allopurinol (10 µg/mL) and A. minus crude extract (10-300 µg/mL) inhibited the xanthine oxidase activity in vitro. The A. minus seeds crude extract did not cause adverse effects since did not change the toxicological parameters evaluated. A. minus crude extract can be used as an assistant therapy of gout pain, supporting its traditional use, without causing adverse effects.
Assuntos
Analgésicos/farmacologia , Arctium/química , Gota/tratamento farmacológico , Extratos Vegetais/farmacologia , Animais , Anti-Inflamatórios/farmacologia , Modelos Animais de Doenças , Edema/tratamento farmacológico , Edema/metabolismo , Gota/metabolismo , Hiperalgesia/tratamento farmacológico , Hiperalgesia/metabolismo , Inflamação/tratamento farmacológico , Inflamação/metabolismo , Masculino , Camundongos , Xantina Oxidase/metabolismoRESUMO
BACKGROUND: Quinoa (Chenopodium quinoa Willd.) has been traditionally used as a foodstuff in the Andes and it has gained increasing interest in recent years owing to its high nutritional value. The potential of near-infrared spectroscopy (NIRS) for estimating chemical compounds in quinoa was studied because of the possibility of conducting direct measurement without prior sample treatment. RESULTS: The use of NIRS technology with a remote reflectance fiber-optic probe for the analysis of major compounds and mineral composition of 48 quinoa samples was studied. The calibration of the NIRS instrument was conducted using modified partial least squares (MPLS) analysis. This allowed the determination of protein (16.0-20.2 g 100g⻹), crude fiber (1.8-3.1 g 100g⻹), fat (4.4-7.5 g 100g⻹), calcium (298.8-1164.5 mg kg⻹), iron (0-948.5 mg kg⻹) and phosphorus (2735.0-4543.3 mg kg⻹). The correlation coefficients (RSQ) were 0.83 for protein, 0.73 for crude fiber, 0.93 for fat; 0.60 for calcium; 0.76 for iron and 0.75 for phosphorus. The robustness of the equations obtained was verified by external validation on unknown quinoa samples. CONCLUSIONS: NIRS with fiber-optic probe provides an alternative for the determination of chemical compounds of quinoa, faster and at lower cost, with results comparable with chemical methods.
Assuntos
Cálcio da Dieta/análise , Chenopodium quinoa/química , Inspeção de Alimentos/métodos , Qualidade dos Alimentos , Ferro da Dieta/análise , Fósforo na Dieta/análise , Sementes/química , Calibragem , Fenômenos Químicos , Chile , Gorduras na Dieta/análise , Fibras na Dieta/análise , Proteínas Alimentares/análise , Tecnologia de Fibra Óptica , Humanos , Análise dos Mínimos Quadrados , Valor Nutritivo , Proteínas de Plantas/análise , Especificidade da Espécie , Espectroscopia de Luz Próxima ao InfravermelhoRESUMO
Boeremia exigua var. exigua is a recurrent pathogen causing root rot in industrial chicory. Currently, there is no chemical or varietal control for this disease, and thus, management strategies need to be developed. This study determined the biocontrol effect of strains of Pseudomonas protegens bacteria with antimicrobial compounds on the fungus B. exigua var. exigua under in vitro, in vivo, and field conditions. In addition, root colonization by these bacteria was estimated by the phlD-specific PCR-based dilution end point assay. Eighteen isolates of Pseudomonas spp were evaluated, and the strains that showed the greatest in vitro inhibition of fungal mycelial growth (mm), Ca10A and ChB7, were selected. Inoculation with the strain ChB7 showed less severity (necrotic area) under in vivo conditions (root trials) compared with the control inoculated with the pathogen (p ≤ 0.05). The molecular analysis revealed that the root colonization of plants grown in pots was equal to or greater than 70%. Similar levels were observed in the field trials conducted at the Selva Negra and Canteras experimental stations (2015-2016 season), with values ranging from 85.7 to 70.5% and from 75.0 to 79.5%, respectively. Regarding yield (ton ha-1), values were higher in the treatments inoculated with strains Ca10A and ChB7 (p ≤ 0.05) at both experimental sites, while a lower incidence and severity of root rot were observed at Selva Negra. These results suggest that the Chilean strains of P. protegens are a promising tool for the control of root diseases in industrial chicory.
RESUMO
Rice is an essential diet component for a significant portion of the population worldwide. Due to the high water demand associated with rice production, improving water use efficiency and grain quality is critical to increasing the sustainability of the crop. This species includes rice varieties with diverse pigmentation patterns. Grain quality, including industrial, nutritional, and functional quality traits, of two black rice genotypes and a commercial white rice cultivar were evaluated in different locations and under different water regimes. Flooding produced higher grain weight compared to alternate wetting and drying irrigation. A high correlation was found between grain color, total phenolic content (TPC), and antioxidant activity. The black rice genotypes showed higher TPC levels and antioxidant capacity, mainly due to higher levels of cyanidin 3-O-glucoside. The phenolic profile varied between whole and polished grains, while mineral composition was influenced by location and irrigation regime. In turn, the environment influenced grain quality in terms of industrial and nutritional characteristics, with significant differences in quality between whole and polished grains. This study provides valuable information on the genotype-environment relationship in rice and its effect on grain quality, which could contribute to selecting genotypes for an appropriate environment.
RESUMO
Chenopodium quinoa Willd. is a native species that originated in the High Andes plateau (Altiplano) and its cultivation spread out to the south of Chile. Because of the different edaphoclimatic characteristics of both regions, soils from Altiplano accumulated higher levels of nitrate (NO3-) than in the south of Chile, where soils favor ammonium (NH4 +) accumulation. To elucidate whether C. quinoa ecotypes differ in several physiological and biochemical parameters related to their capacity to assimilate NO3- and NH4 +, juvenile plants of Socaire (from Altiplano) and Faro (from Lowland/South of Chile) were grown under different sources of N (NO3- or NH4 +). Measurements of photosynthesis and foliar oxygen-isotope fractionation were carried out, together with biochemical analyses, as proxies for the analysis of plant performance or sensitivity to NH4 +. Overall, while NH4 + reduced the growth of Socaire, it induced higher biomass productivity and increased protein synthesis, oxygen consumption, and cytochrome oxidase activity in Faro. We discussed that ATP yield from respiration in Faro could promote protein production from assimilated NH4 + to benefit its growth. The characterization of this differential sensitivity of both quinoa ecotypes for NH4 + contributes to a better understanding of nutritional aspects driving plant primary productivity.
RESUMO
"Memory imprint" refers to the process when prior exposure to stress prepares the plant for subsequent stress episodes. Seed priming is a strategy to change the performance of seedlings to cope with stress; however, mechanisms associated with the metabolic response are fragmentary. Salinity is one of the major abiotic stresses that affect crop production in arid and semiarid areas. Chenopodium quinoa Willd. (Amaranthaceae) is a promising crop to sustain food security and possesses a wide genetic diversity of salinity tolerance. To elucidate if the metabolic memory induced by seed halo-priming (HP) differs among contrasting saline tolerance plants, seeds of two ecotypes of Quinoa (Socaire from Atacama Salar, and BO78 from Chilean Coastal/lowlands) were treated with a saline solution and then germinated and grown under different saline conditions. The seed HP showed a more positive impact on the sensitive ecotype during germination and promoted changes in the metabolomic profile in both ecotypes, including a reduction in carbohydrates (starch) and organic acids (citric and succinic acid), and an increase in antioxidants (ascorbic acid and α-tocopherol) and related metabolites. These changes were linked to a further reduced level of oxidative markers (methionine sulfoxide and malondialdehyde), allowing improvements in the energy use in photosystem II under saline conditions in the salt-sensitive ecotype. In view of these results, we conclude that seed HP prompts a "metabolic imprint" related to ROS scavenger at the thylakoid level, improving further the physiological performance of the most sensitive ecotype.
RESUMO
Fibromyalgia is characterised mainly by symptoms of chronic widespread pain and comorbidities like depression. Although these symptoms cause a notable impact on the patient's quality of life, the underlying aetiology and pathophysiology of this disease remain incompletely elucidated. The transient receptor potential vanilloid type 1 (TRPV1) is a polymodal receptor that is involved in the development of nociceptive and depressive behaviours, while α-spinasterol, a multitarget TRPV1 antagonist and cyclooxygenase inhibitor, presents antinociceptive and antidepressant effects. The present study investigated the involvement of the TRPV1 channel and the possible effects of α-spinasterol on nociceptive and depressive-like behaviours in an experimental fibromyalgia model. The fibromyalgia model was induced with a subcutaneous (s.c.) injection of reserpine (1 mg/kg) once daily for 3 consecutive days in male Swiss mice. Reserpine administration depleted monoamines and caused mechanical allodynia. This dysfunction was inhibited by SB-366791 (1 mg/kg, oral route [p.o.]), a selective TRPV1 antagonist, with a maximum inhibition (Imax) of 73.4 ± 15.5%, or by the single or 3-day-repeated administration of α-spinasterol (0.3 mg/kg, p.o.), with an Imax of 72.8 ± 17.8% and 78.9 ± 32.9%, respectively. SB-366791 also inhibited the increase of the reserpine-induced immobility time, with an Imax of 100%, while α-spinasterol inhibited this parameter with an Imax of 98.2 ± 21.5% and 100%, by single or repeated administration, respectively. The reserpine-induced mechanical allodynia and the thermal hyperalgesia were abolished by TRPV1-positive fibers desensitization induced by previous resiniferatoxin (RTX) administration. In summary, the TRPV1 channel is involved in the development and maintenance of nociception and depressive-like behaviours in a fibromyalgia model, while the α-spinasterol has therapeutic potential to treat the pain and depression symptoms in fibromyalgia patients.
Assuntos
Fibromialgia/tratamento farmacológico , Hiperalgesia/tratamento farmacológico , Estigmasterol/análogos & derivados , Canais de Cátion TRPV/efeitos dos fármacos , Animais , Antidepressivos/farmacologia , Dor Crônica/tratamento farmacológico , Modelos Animais de Doenças , Masculino , Camundongos , Medição da Dor/efeitos dos fármacos , Qualidade de Vida , Estigmasterol/farmacologia , Canais de Cátion TRPV/metabolismoRESUMO
Fibromyalgia (FM) is one of the most common musculoskeletal pain conditions. Although the aetiology of FM is still unknown, mitochondrial dysfunction and the overproduction of reactive oxygen intermediates (ROI) are common characteristics in its pathogenesis. The reserpine experimental model can induce FM-related symptoms in rodents by depleting biogenic amines. However, it is unclear whether reserpine causes other pathophysiologic characteristics of FM. So far, no one has investigated the relevance of mitochondrial dysfunction in the reserpine-induced experimental FM model using protection- and insult-based mitochondrial modulators. Reserpine (1 mg/kg) was subcutaneously injected once daily for three consecutive days in male Swiss mice. We carried out analyses of reserpine-induced FM-related symptoms, and their modulation by using mitochondrial insult on ATP synthesis (oligomycin; 1 mg/kg, intraperitoneally) or mitochondrial protection (coenzyme Q10; 150 mg/kg/5 days, orally). We also evaluated the effect of reserpine on mitochondrial function using high-resolution respirometry and oxidative status. Reserpine caused nociception, loss in muscle strength, and anxiety- and depressive-like behaviours in mice that were consistent with clinical symptoms of FM, without inducing body weight and temperature alterations or motor impairment. Reserpine-induced FM-related symptoms were increased by oligomycin and reduced by coenzyme Q10 treatment. Reserpine caused mitochondrial dysfunction by negatively modulating the electron transport system and mitochondrial respiration (ATP synthesis) mainly in oxidative muscles and the spinal cord. These results support the role of mitochondria in mediating oxidative stress and FM symptoms in this model. In this way, reserpine-inducing mitochondrial dysfunction and increased production of ROI contribute to the development and maintenance of nociceptive, fatigue, and depressive-like behaviours.
Assuntos
Fibromialgia/induzido quimicamente , Fibromialgia/patologia , Mitocôndrias/patologia , Reserpina/efeitos adversos , Animais , Comportamento Animal , Depressão/complicações , Depressão/fisiopatologia , Modelos Animais de Doenças , Fadiga/complicações , Fadiga/fisiopatologia , Fibromialgia/fisiopatologia , Masculino , Camundongos , Mitocôndrias/efeitos dos fármacos , Mitocôndrias/metabolismo , Modelos Biológicos , Músculos/efeitos dos fármacos , Músculos/patologia , Nociceptividade/efeitos dos fármacos , Oxirredução , Medula Espinal/efeitos dos fármacos , Medula Espinal/patologia , Ubiquinona/análogos & derivados , Ubiquinona/metabolismoRESUMO
Muscle pain is the most prevalent type of pain in the world, but treatment remains ineffective. Thus, it is relevant to develop trustable animal models to understand the involved pain mechanisms. Therefore, this study characterised the nociception and inflammation in a traumatic muscle injury model in rats. A single blunt trauma impact on the right gastrocnemius muscle of male Wistar rats (250-350 g) was used as model for muscle pain. Animals were divided into four groups (sham/no treatment; sham/diclofenac 1%; injury/no treatment; injury/diclofenac 1%) and the topical treatment with a cream containing 1% monosodium diclofenac (applied at 2, 6, 12, 24, and 46 h after muscle injury; 200 mg/muscle) was used as an anti-inflammatory control. Nociception (mechanical and cold allodynia, or nociceptive score) and locomotor activity were evaluated at 26 and 48 h after injury. Also, inflammatory and oxidative parameters were evaluated in gastrocnemius muscle and the creatine kinase (CK) activity and lactate/glicose levels in rat's serum and plasma, respectively. Muscle injury caused mechanical and cold allodynia, and increased nociceptive scores, without inducing locomotor impairment. This model also increased the inflammatory cells infiltration (seen by myeloperoxidase and N-acetyl-ß-D-glucosaminidase activities and histological procedure), nitric oxide, interleukin (IL)-1ß, IL-6, and dichlorofluorescein fluorescence in muscle samples; and CK activity and lactate/glicose ratio. The treatment with 1% monosodium diclofenac reduced inflammatory cells infiltration, dichlorofluorescein fluorescence and lactate/glicose levels. Thus, we characterised the traumatic muscle injury as a reproducible model of muscle pain, which makes it possible to evaluate promising antinociceptive and anti-inflammatory therapies.
Assuntos
Inflamação , Dor Musculoesquelética , Nociceptividade , Dor Nociceptiva , Ferimentos não Penetrantes , Administração Tópica , Analgésicos/administração & dosagem , Animais , Anti-Inflamatórios não Esteroides/administração & dosagem , Comportamento Animal , Citocinas/metabolismo , Diclofenaco/administração & dosagem , Modelos Animais de Doenças , Inflamação/tratamento farmacológico , Inflamação/metabolismo , Inflamação/fisiopatologia , Mediadores da Inflamação/metabolismo , Locomoção , Masculino , Músculo Esquelético/efeitos dos fármacos , Músculo Esquelético/lesões , Músculo Esquelético/metabolismo , Músculo Esquelético/fisiopatologia , Dor Musculoesquelética/tratamento farmacológico , Dor Musculoesquelética/metabolismo , Dor Musculoesquelética/fisiopatologia , Nociceptividade/efeitos dos fármacos , Dor Nociceptiva/tratamento farmacológico , Dor Nociceptiva/metabolismo , Dor Nociceptiva/fisiopatologia , Estresse Oxidativo , Ratos Wistar , Ferimentos não Penetrantes/tratamento farmacológico , Ferimentos não Penetrantes/metabolismo , Ferimentos não Penetrantes/fisiopatologiaRESUMO
Fibromyalgia is a disease characterised as generalised chronic primary pain that causes functional disability and a reduction in patients' quality of life, without specific pathophysiology or appropriate treatment. Previous studies have shown that kinins and their B1 and B2 receptors contribute to chronic painful conditions. Thus, we investigated the involvement of kinins and their B1 and B2 receptors in a fibromyalgia-like pain model induced by reserpine in mice. Nociceptive parameters (mechanical allodynia, cold sensitivity and overt nociception) and behaviours of burrowing, thigmotaxis, and forced swimming were evaluated after reserpine administration in mice. The role of kinin B1 and B2 receptors was investigated using knockout mice or pharmacological antagonism. The protein expression of kinin B1 and B2 receptors and the levels of bradykinin and monoamines were measured in the sciatic nerve, spinal cord and cerebral cortex of the animals. Knockout mice for the kinin B1 and B2 receptor reduced reserpine-induced mechanical allodynia. Antagonism of B1 and B2 receptors also reduced mechanical allodynia, cold sensitivity and overt nociception reserpine-induced. Reserpine altered thigmotaxis, forced swimming and burrowing behaviour in the animals; with the latter being reversed by antagonism of kinin B1 receptor. Moreover, reserpine increased the protein expression of kinin B1 and B2 receptors and levels of kinin, as well as reduced the levels of monoamines in peripheral and central structures. Kinins and its B1 and B2 receptors are involved in fibromyalgia-like pain symptoms. B1 or B2 receptors might represent a potential target for the relief of fibromyalgia-like pain symptoms.
Assuntos
Bradicinina/metabolismo , Fibromialgia/metabolismo , Dor/metabolismo , Receptor B1 da Bradicinina/metabolismo , Receptor B2 da Bradicinina/metabolismo , Animais , Comportamento Animal/efeitos dos fármacos , Modelos Animais de Doenças , Fibromialgia/induzido quimicamente , Técnicas de Inativação de Genes , Hiperalgesia/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Nociceptividade/efeitos dos fármacos , Dor/induzido quimicamente , Receptor B1 da Bradicinina/genética , Receptor B2 da Bradicinina/genética , Reserpina/farmacologiaRESUMO
Quinoa is a pseudocereal that is grown mainly in the Andes. It is a functional food supplement and ingredient in the preparation of highly nutritious food. In this paper we evaluate the potential of near infrared spectroscopy (NIR) for the determination of vitamin E and antioxidant capacity in the quinoa as total phenol content (TPC), radical scavenging activity by DPPH (2,2-diphenyl-2-picryl-hydrazyl) and cupric reducing antioxidant capacity (CUPRAC) expressed as gallic acid equivalent (GAE). For recording NIR a fiber optic remote reflectance probe applied directly on the quinoa samples without treatment was used. The regression method used was modified partial least squares (MPLS). The multiple correlation coefficients (RSQ) and the standard prediction error corrected (SEP(C)) were for the vitamin E (0.841 and 1.70 mg 100 g(-1)) and for the antioxidants TPC (0.947 and 0.08 mg GAE g(-1)), DPPH radical (0.952 and 0.23 mg GAE g(-1)) and CUPRAC ( 0.623 and 0.21 mg GAE g(-1)), respectively. The prediction capacity of the model developed measured by the ratio performance deviation (RPD) for vitamin E (2.51), antioxidants TPC (4.33), DPPH radical (4.55) and CUPRAC (1.55) indicated that NIRS with a fiber optic probe provides an alternative for the determination of vitamin E and antioxidant properties of the quinoa, with a lower cost, higher speed and results comparable with the chemical methods.
Assuntos
Antioxidantes/análise , Chenopodium quinoa/química , Vitamina E/análise , Ácido Ascórbico/química , Compostos de Bifenilo/química , Calibragem , Cobre/química , Ácido Gálico/química , Análise dos Mínimos Quadrados , Fenóis/química , Picratos/química , Espectroscopia de Luz Próxima ao InfravermelhoRESUMO
Chenopodium quinoa W. is a pseudocereal with bioactive compounds like polyphenols, carotenoids, dietary fibers and oleic acid, which have acquired importance because of their human health benefits. The present study aimed to determine the effect of controlled water restriction on the potential yield, chemical composition (protein, fat content and crude fiber) and antioxidant capacity in seeds of three genotypes of quinoa. The study was conducted in the south-central zone of Chile under field and controlled conditions in a greenhouse. Main plot treatment was available water level and subplots included three quinoa genotypes. Results indicated an increase of the antioxidant capacity, with an average of 88 percent in seeds of the three genotypes and 70 percent in seeds of plants exposed to 95 to 20 percent available water. Seed yield potential was reduced, but the extent of reduction varied depending on the genotype. It was possible to produce seeds of higher nutritional value when controlled water stress was applied from 40 to 20 percent available water, without a considerably reduction on seed yield.
Chenopodium quinoa W. es un pseudocereal con sustancias bioactivas como polifenoles, carotenoides, fibras dietarias y ácido oleico, las que han adquirido importancia, principalmente debido a los beneficios que produce en la salud humana. El propósito de este estudio fue en semilla determinar el efecto de la restricción hídrica controlada sobre el potencial de rendimiento, la composición química (proteína, contenido de grasas, fibra cruda) y la capacidad antioxidante, de tres genotipos de quinoa. Este estudio se realizó en la zona centro sur de Chile, en condiciones de campo y en invernadero, en condiciones controladas. El tratamiento principal fue la disponibilidad de agua y las subparcelas los genotipos de quinoa. Se observó en los resultados un incremento en la capacidad antioxidante de un 88 por ciento entre genotipos y un 70 por ciento en semillas expuestas desde 95 a 20 por ciento de la capacidad de campo. Por otra parte el potencial de rendimiento se redujo en diferentes magnitudes entre genotipos. Finalmente, fue posible producir semillas con mayor valor nutritivo cuando se aplicó una restricción hídrica desde un 40 a un 20 por ciento de la capacidad de campo sin reducir considerablemente el rendimiento.