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BACKGROUND/OBJECTIVES: We have reported that maternal overnutrition/obesity (OB) in sheep resulting from feeding 150% of National Research Council (NRC) requirements throughout gestation leads to maternal hyperglycemia and hyperinsulinemia. Further, newborn lambs born to OB vs control-fed (CON, 100% of NRC) ewes exhibited greater adiposity, increased blood cortisol, insulin and glucose and the elimination of the postnatal leptin spike seen in lambs born to CON ewes. This early postnatal leptin peak is necessary for the development of hypothalamic circuits, which program appetite in later life. This study evaluated the multigenerational impact of OB on insulin:glucose dynamics of mature female F1 offspring fed only to requirements throughout gestation and on their lambs (F2 generation). DESIGN AND METHODS: Adult F1 female offspring born to OB (n=10) or CON (n=7) ewes were utilized. All F1 ewes were subjected to a glucose tolerance test at midgestation and late gestation. Jugular blood was obtained from F2 lambs at birth (day 1) through postnatal day 11, and plasma glucose, insulin, cortisol and leptin concentrations were determined. Dual-energy X-ray absorptiometry was utilized to determine bone mineral density, bone mineral content, lean tissue mass and fat tissue mass. RESULTS: Fasted blood glucose and insulin concentrations were greater (P<0.05) in OBF1 than CONF1 ewes at midgestation and late gestation. Further, after glucose infusion, both glucose and insulin concentrations remained higher in OBF1 ewes (P<0.05) than CONF1 ewes, demonstrating greater insulin resistance. Blood concentrations of glucose, insulin and cortisol and adiposity were higher (P<0.01) in OBF2 lambs than CONF2 lambs at birth. Importantly, OBF2 lambs failed to exhibit the early postnatal leptin peak exhibited by CONF2 lambs. CONCLUSIONS: These data suggest that these OBF2 lambs are predisposed to exhibit the same metabolic alterations as their mothers, suggesting a multigenerational programming effect.
Assuntos
Fenômenos Fisiológicos da Nutrição Animal/fisiologia , Glicemia/metabolismo , Leptina/metabolismo , Obesidade/patologia , Efeitos Tardios da Exposição Pré-Natal/metabolismo , Ração Animal , Animais , Feminino , Teste de Tolerância a Glucose , Masculino , Desnutrição , Fenômenos Fisiológicos da Nutrição Materna/fisiologia , Hipernutrição , Gravidez , OvinosRESUMO
BACKGROUND: Obesity in women of childbearing age is increasing at an alarming rate. Growing evidence shows that maternal obesity induces detrimental effects on offspring health, including pre-disposition to obesity. We have shown that maternal obesity increases fetal intramuscular adipogenesis at mid-gestation. However, the mechanisms are poorly understood. MicroRNAs (miRNAs) regulate mRNA stability. We hypothesized that maternal obesity alters fetal muscle miRNA expression, thereby influencing intramuscular adipogenesis. METHODS: Non-pregnant ewes received a control diet (Con, fed 100% of National Research Council (NRC) recommendations, n=6) or obesogenic diet (OB; 150% NRC recommendations, n=6) from 60 days before to 75 days after conception when the fetal longissimus dorsi (LD) muscle was sampled and miRNA expression analyzed by miRNA microarray. One of miRNAs with differential expression between Con and OB fetal muscle, let-7g, was further tested for its role in adipogenesis and cell proliferation in C3H10T1/2 cells. RESULTS: A total of 155 miRNAs were found with a signal above 500, among which, three miRNAs, hsa-miR-381, hsa-let-7g and bta-miR-376d, were differentially expressed between Con and OB fetuses, and confirmed by quantitative real-time PCR (QRT-PCR) analyses. Reduced expression of miRNA let-7g, an abundantly expressed miRNA, in OB fetal muscle was correlated with higher expression of its target genes. Overexpression of let-7g in C3H10T1/2 cells reduced their proliferation rate. Expression of adipogenic markers decreased in cells overexpressing let-7g, and the formation of adipocytes was also reduced. Overexpression of let-7g decreased expression of inflammatory cytokines. CONCLUSION: Fetal muscle miRNA expression was altered due to maternal obesity, and let-7g downregulation may enhance intramuscular adipogenesis during fetal muscle development in the setting of maternal obesity.
Assuntos
Adipogenia/genética , Desenvolvimento Fetal/genética , MicroRNAs/metabolismo , Músculo Esquelético/metabolismo , Obesidade/metabolismo , Animais , Dieta , Regulação para Baixo , Feminino , Regulação da Expressão Gênica no Desenvolvimento , Fenômenos Fisiológicos da Nutrição Materna , Músculo Esquelético/embriologia , Obesidade/genética , Gravidez , Reação em Cadeia da Polimerase em Tempo Real , Carneiro DomésticoRESUMO
The goal of this review is to shed light on the role of maternal malnutrition in inducing epigenetic changes in gene expression, leading to alterations in fetal growth and development, and to altered postnatal phenotype and the development of metabolic disease. We present evidence supporting the concept that both maternal undernutrition and overnutrition can induce the same cadre of fetal organ and tissue abnormalities and lead to the same postnatal metabolic changes in the resulting offspring. Furthermore, we present evidence that in both overnourished and undernourished ovine pregnancies, fetuses experience a period of nutrient restriction as a result of alterations in placental delivery of maternal nutrients into the fetal compartment. We argue that this bout of reduced fetal nutrition in undernourished and overnourished pregnancies leads to the development of a thrifty phenotype in which the fetus attempts to alter the function of its tissues and organs to maximise its chances of survival in a postnatal environment that is deficient in nutrients. Importantly, we present evidence to support the concept that these phenotypic changes in offspring quality resulting from maternal malnutrition are transmitted to subsequent generations, independent of their maternal nutritional inputs.
Assuntos
Epigênese Genética/fisiologia , Desenvolvimento Fetal/fisiologia , Desnutrição/fisiopatologia , Hipernutrição/fisiopatologia , Fenótipo , Animais , Feminino , Regulação da Expressão Gênica no Desenvolvimento/fisiologia , Humanos , Leptina/fisiologia , Fenômenos Fisiológicos da Nutrição Materna/fisiologia , Modelos Animais , Gravidez , OvinosRESUMO
Accumulating evidence suggests that indications of metabolic syndrome can be inherited through the germline as a result of maternal obesity. We hypothesized that diet-induced maternal obesity during gestation would program metabolic consequences for multiple generations of offspring, even when first, second, and third generation offspring (F1, F2, F3, respectively) were fed only to requirements. Control (CON) and obese (OB) ewes (generation 0; F0) were bred to a single ram to produce the first generation of offspring (F1). From 60 d prior to conception through term, CONF0 ate 100% National Research Council recommendations (NRC), while OBF0 ewes ate 150% NRC. All F1, F2, and F3 ate 100% NRC after weaning. All mature F1 ewes were bred to a single ram to generate CONF2 (n = 6) and OBF2 (n = 10). All mature F2 ewes were bred to a single ram to produce CONF3 (n = 6) and OBF3 (n = 10). OBF2 ewes exhibited greater (P < 0.0001) plasma cortisol than CONF2 throughout gestation. A glucose tolerance test at 90% gestation revealed OBF2 ewes had higher (P < 0.05) insulin response with similar glucose, resulting in greater (P < 0.05) insulin resistance. OBF3 neonates had similar weight, lean mass, and body fat mass to CONF3 neonates. These data suggest that multigenerational programming of adverse metabolic phenotypes occur in association with F0 maternal obesity, yet adiposity may return to CON levels in F3 neonates.
Assuntos
Obesidade Materna , Efeitos Tardios da Exposição Pré-Natal , Doenças dos Ovinos , Animais , Glicemia/metabolismo , Feminino , Teste de Tolerância a Glucose/veterinária , Humanos , Masculino , Obesidade/metabolismo , Obesidade/veterinária , Obesidade Materna/veterinária , Gravidez , Efeitos Tardios da Exposição Pré-Natal/veterinária , OvinosRESUMO
Marbling, or intramuscular fat, is an important factor in meat quality. As a key regulator of lipid metabolism, AMP activated protein kinase (AMPK) may be associated with intramuscular fat accumulation. Our objective was to evaluate the relationship among AMPK and its associated signaling mediators, with marbling and lean growth in beef cattle. Steers with high intramuscular fat content (High IMF, 5.71±0.36%, n=5) and low intramuscular fat content (Low IMF, 2.09±0.19%, n=5) were selected. High IMF was associated with increased tenderness (P<0.05) and backfat thickness (P<0.01). Muscle weights were higher in Low compared to High IMF (P<0.05). High IMF steers had a reduced AMPK activity (P<0.01), reduced acetyl-CoA carboxylase phosphorylation (P<0.05), and reduced total mTOR (P=0.02) content. Data provide evidence that AMPK is involved in IMF deposition in beef cattle.
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Adiponectin potentially influences fetal weight by altering insulin signaling and trans-placental amino acid and glucose transporters. The objective of this study was to determine how maternal obesity influences maternal and fetal plasma concentrations of adiponectin, expression of fetal adiponectin, its receptors, and adipogenic genes at mid- and late-gestation. Blood samples and tissues were collected from obese and control multiparous pregnant ewes at day 75 or 135 of gestation. Although day of gestation or maternal obesity did not influence (P > 0.6) maternal plasma concentrations of adiponectin, fetal weight was increased (P < 0.001) and adiponectin tended to decrease (P = 0.10) at mid-gestation in fetuses from obese ewes. Differences were not apparent at late-gestation (P > 0.70). Relative abundance of adiponectin (P = 0.01), AdipoR2 (P = 0.04) and PPARγ (P = 0.01) mRNA was less at mid-gestation in fetal adipose tissue from obese mothers. By late gestation, maternal obesity tended to associated with a decrease in relative abundance of adiponectin (P = 0.09) and SREBF1 (P = 0.10) mRNA in fetal adipose tissue. Maternal obesity did not influence (P ≥ 0.20) the relative abundance of adiponectin, AdipoR1 and AdipoR2 mRNA in cotyledonary tissue at mid or late- gestation. In conclusion, maternal obesity in sheep influences relative abundance of fetal adipose tissue mRNA for adiponectin and adipogenic, as well as plasma concentrations of total adiponectin. Although adiposity in pregnant ewes did not influence maternal adiponectin, maternal obesity potentially influenced fetal adipogenesis by altering the abundance of adiponectin, PPARγ and SREBF1 mRNA in fetal adipose tissue.
Assuntos
Adiponectina/metabolismo , Feto/metabolismo , Obesidade/veterinária , Doenças dos Ovinos/metabolismo , Tecido Adiposo/química , Tecido Adiposo/metabolismo , Animais , Feminino , Regulação da Expressão Gênica no Desenvolvimento/fisiologia , Obesidade/metabolismo , Placenta , Gravidez , OvinosRESUMO
This study evaluated the role of MAPK/ERK1/2 and/or PI3K/Akt signaling pathways in modulating ovine placentomal vascularity in response to periconceptional maternal nutrient restriction. Ewes were randomly assigned to be nutrient restricted (NR, 50% NRC recommendation, N=7) or control fed (CF, 100% NRC recommendation, N=7) from 60 +/- 2 days before to 30 days after conception (day 0). From day 31 of gestation, all ewes (CF and NR) were fed the control diet until necropsy on day 78. On day 78 of gestation, NR ewes exhibited greater vascularity in both caruncular (CAR) and cotyledon (COT) tissues than CF ewes. Akt or ERK1/2 content in CAR and COT arterial tissue did not differ across dietary treatment. The activated forms, phosphorylated Akt and phosphorylated ERK1/2, were significantly increased in COT but not CAR arterial tissues of NR ewes compared to those of CF ewes (P<0.05). For both CF and NR ewes, phosphorylated Akt and phosphorylated ERK1/2 content in COT are higher (P<0.05) than those in CAR arterial tissues. Immunohistochemical staining revealed cytoplasmic and nuclear localization of Akt, phosphorylated Akt, ERK1/2 and phosphorylated ERK1/2, with phosphorylated Akt and phosphorylated-ERK1/2 specifically localized in trophoblast cells, while binucleate cells remained unstained. In placentomal blood vessels, Akt, phosphorylated Akt, ERK1/2 and phosphorylated ERK1/2 were localized to both endothelium and smooth muscle cells. These findings demonstrate for the first time that periconceptional NR increases vascular density in both COT than CAR tissues of the ovine placentome, and that the MAPK/ERK1/2 and/or PI3K/Akt signaling pathways are increased in NR COT but not NR CAR arterial tissues.
Assuntos
Fenômenos Fisiológicos da Nutrição Animal/fisiologia , Proteína Quinase 1 Ativada por Mitógeno/metabolismo , Proteína Quinase 3 Ativada por Mitógeno/metabolismo , Fosfatidilinositol 3-Quinases/metabolismo , Placenta/irrigação sanguínea , Proteínas Proto-Oncogênicas c-akt/metabolismo , Animais , Restrição Calórica , Feminino , Peso Fetal/fisiologia , Imuno-Histoquímica , Tamanho do Órgão/fisiologia , Placenta/química , Placentação , Gravidez , Ovinos , Transdução de Sinais/fisiologiaRESUMO
This study evaluated the role of MAPK/ERK1/2 and/or PI3-K/Akt signaling pathways in modulating bovine placentomal vascularity in response to maternal nutrient restriction. Beef cows were randomly assigned to control fed (Control, n=15, 100% of requirements) or nutrient restricted (NR, n=15, 50% requirements) diets from day 30 to day 125 of gestation. Ten cows from each dietary group were necropsied on day 125 (approximately 45% gestation), and the remaining cows in each diet group were then fed control diets and necropsied on day 250 (approximately 90% gestation). At day 125 of gestation, NR cows exhibited increased (P=0.06) COT vascularity, improved (P<0.05) placentome efficiency (fetal weight/placentomal weight), and increased (P<0.05) phosphorylated Akt and ERK1/2 in COT arteries compared to Control cows. By day 250, however, treatment differences in COT vascularity and phosphorylated Akt and ERK1/2 in COT arteries were lost. On both gestational days, no treatment difference was observed in the levels of phosphorylated Akt or ERK1/2 in CAR arteries. CAR vascularity was similar across treatment on day 125, but tended to be greater (P<0.10) in NR than Control cows on day 250. These data suggest that conceptuses react to an early gestational nutrient restriction by up-regulating COT growth signaling pathways associated with angiogenesis, and that these compensations do not persist to term.
Assuntos
Fenômenos Fisiológicos da Nutrição Animal , MAP Quinases Reguladas por Sinal Extracelular/metabolismo , Privação de Alimentos/fisiologia , Placenta/irrigação sanguínea , Circulação Placentária/fisiologia , Animais , Bovinos , Feminino , Peso Fetal , Idade Gestacional , Neovascularização Fisiológica/fisiologia , Tamanho do Órgão , Fosfatidilinositol 3-Quinases/metabolismo , Fosforilação , Placentação , Gravidez , Transdução de SinaisRESUMO
We previously reported that maternal overnutrition and obesity (MO) throughout pregnancy and lactation in sheep (MOF0) decreases term fetal pancreatic ß-cell numbers and increases perirenal adiposity producing hyperphagia, increased adiposity and insulin resistance in adult female offspring (MOF1) fed ad libitum. Pregnant female MOF1 exhibited increased blood glucose from mid to late gestation vs control F1 (CTRF1) though both groups ate only to NRC recommendations. MOF1 ewes delivered female offspring (F2) who like their MOF1 mothers exhibited increased abdominal adiposity and absent neonatal leptin surge. In the current work, we determined if adult MOF2 exhibited metabolic syndrome components when fed ad libitum. After weaning, MOF2 males (n = 5), MOF2 females (n = 6), CTRF2 males (n = 5), and CTRF2 females (n = 6) were fed to NRC requirements until 19 mo followed by 12-wk ad libitum feeding. Body weight and % fat increased (P < 0.01) in all F2 during this feeding trial. MOF2 males were heavier (P < 0.01) than CTRF2 males and females, and MOF2 females throughout the trial. By wk 8, baseline blood glucose concentrations increased (P < 0.001) in MOF2 females, but not other groups, remaining elevated throughout the trial. Baseline insulin was similar through wk 6, increasing (P < 0.05) at wk 8 in MOF2 females only. MOF2 female insulin returned to CTRF2 female levels during wk 10 and 12. The progressive increase of plasma glucose on wk 8 in association with increased insulin in MOF2 females but not other groups demonstrated a diet-induced increase (P < 0.001) in MOF2 female insulin resistance. The subsequent decline in insulin during wk 10 and 12 despite elevated glucose in MOF2 females is consistent with a decrease in glucose-stimulated pancreatic ß-cell function. These data indicate that ad libitum feeding exceeds the pancreatic secretory response predisposing MOF2 females to hyperglycemia. Furthermore, there was a sex difference where MOF2 males increased body mass and MOF2 females displayed insulin/glucose dysregulation.
Assuntos
Fenômenos Fisiológicos da Nutrição Animal , Síndrome Metabólica/veterinária , Obesidade/veterinária , Hipernutrição , Fenômenos Fisiológicos da Nutrição Pré-Natal , Doenças dos Ovinos/etiologia , Ração Animal , Animais , Glicemia , Composição Corporal , Feminino , Insulina/sangue , Masculino , Síndrome Metabólica/etiologia , Gravidez , Efeitos Tardios da Exposição Pré-Natal , Ovinos , Aumento de PesoRESUMO
Numerous studies have revealed that pre-slaughter stress, like transport, increases the occurrence of pale, soft, and exudative (PSE) pork meat. The molecular mechanism underlying this phenomenon, however, is poorly defined. In this study, we investigated the effects of pre-slaughter transport and subsequent rest on energy metabolism, AMP-activated protein kinase (AMPK) activation, and glycolysis in postmortem pork loin. Results indicated that pre-slaughter transport accelerated ATP depletion, which led to lower energy status in postmortem muscle immediately post-exsanguination when compared with control. The lower energy status led to AMPK activation within 1h postmortem, subsequently increasing glycolysis, leading to rapid glycolysis and high incidence of PSE meat. Allowing pigs to rest after transport restored energy status in muscle ante-mortem. Higher energy status then prevented premature and rapid AMPK activation in postmortem muscle and lessened the negative effects of pre-slaughter transport on meat quality. AMPK regulated glycolysis in postmortem muscle, at least partially, through phosphorylation and activation of phosphofructose kinase-2, since fructose-2,6-diphosphate content, an allosteric activator of phosphofructose kinase-1, was well correlated with AMPK activation and glycolytic rate. This suggests that AMPK is a potential molecular target for the control of PSE incidence in pork.
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The following studies were performed to investigate levels of expression of steroidogenic enzymes in porcine conceptuses between days 12 and 21 postmating and to correlate these findings with estrogen levels in conceptus tissues. In the first experiment, levels of steroidogenic enzymes in individual day 12 blastocysts were examined by Western immunoblot analyses. In a second experiment, Northern blot, slot blot, and Western immunoblot analyses for 17 alpha-hydroxylase cytochrome P450 (P450(17) alpha) were performed on conceptus tissue pooled for each uterine horn of sows on days 12, 14, 16, and 21 postmating. On day 12, P450(17) alpha) protein was detectable in 6-mm blastocysts, with highest levels apparent in 10- to 15-mm (tubular) blastocysts. Filamentous blastocysts appeared to have less P450(17) alpha) protein than did littermate tubular blastocysts. Side-chain cleavage cytochrome P450 (P450scc) and aromatase cytochrome P450 (P450arom) followed a pattern similar to that of P450(17) alpha. 3 beta-Hydroxysteroid dehydrogenase was undetectable by Western analysis in blastocysts at the stages examined, but was detectable in placenta from fetuses at later stages of gestation. In pooled tissue, P450(17) alpha) protein and mRNA were greater in day 12 conceptuses than in conceptuses from all other days. However, transition from the tubular to the filamentous form on day 12 postmating was associated with a dramatic decline in the level of P450(17) alpha) mRNA. The conceptus 17 beta-estradiol concentration was highly correlated with immunoreactive P450(17) alpha) protein and hybridizable P450(17) alpha) mRNA over days 12, 14, 16, and 21 postmating. These data suggest that the decrease in blastocyst estrogen secretion occurring after the time of elongation in porcine conceptuses may be due to a decrease in P450(17) alpha expression.
Assuntos
Blastocisto/enzimologia , Esteroides/biossíntese , 3-Hidroxiesteroide Desidrogenases/metabolismo , Animais , Aromatase/metabolismo , Northern Blotting , Western Blotting , Enzima de Clivagem da Cadeia Lateral do Colesterol/metabolismo , Idade Gestacional , Hibridização de Ácido Nucleico , RNA/análise , Esteroide 17-alfa-Hidroxilase/genética , Esteroide 17-alfa-Hidroxilase/metabolismo , SuínosRESUMO
Localized expression of cytochromes P450 17 alpha-hydroxylase (P450c17) and aromatase (P450arom) was investigated in embryonic cell layers of elongating porcine blastocysts by immunocytochemistry. Blastocysts were flushed from the uterus on day 12 of pregnancy, fixed in paraformaldehyde, embedded in paraffin, sectioned, and stained using immunogold- and peroxidase-based techniques. Staining for both P450c17 and P450arom was intense in spherical 7- to 10-mm blastocysts, but was absent in earlier stage 2- to 4-mm blastocysts and less intense or absent in later stage 20-mm and filamentous embryos. Cytochrome P450c17 was limited to the trophoblast of all blastocysts expressing the enzyme, and in spherical 7- to 10-mm blastocysts, essentially all cells of the trophoblast layer stained positively for P450c17. However, as elongation became apparent in 10-mm blastocysts, the cells of the trophoblast became flattened, and the expression of P450c17 declined particularly in those trophoblast cells adjacent to the embryonic disc where mesoderm outgrowth was occurring. In fact, two distinct populations of trophoblast cells became obvious: one that maintained P450c17 expression, and one that did not. Moreover, those trophoblast cells expressing P450c17 were less flattened than neighboring cells in which P450c17 expression was absent. These two morphologically and functionally distinct trophoblastic cell populations were most obvious in areas furthest from the embryonic disc. Cytochrome P450arom was expressed in the trophoblast as well as the hypoblast under the embryonic disc. Neither P450c17 nor P450arom appeared to be expressed in the embryonic disc or the mesoderm of the expanding blastocyst. These functional and structural changes in the embryonic cell layers of the elongating conceptus may be associated with the transient synthesis and secretion of estrogen that occur at the time of maternal recognition of pregnancy in the pig.
Assuntos
Aromatase/análise , Blastocisto/citologia , Blastocisto/enzimologia , Embrião de Mamíferos/citologia , Embrião de Mamíferos/enzimologia , Esteroide 17-alfa-Hidroxilase/análise , Suínos/embriologia , Animais , Células Cultivadas , Feminino , Imuno-Histoquímica , Masculino , GravidezRESUMO
Localized expression of cytochromes P450 17 alpha-hydroxylase (P450c17) and aromatase (P450arom) was investigated in embryonic cell layers of elongating porcine blastocysts by immunocytochemistry. Blastocysts were flushed from the uterus on day 12 of pregnancy, fixed in paraformaldehyde, embedded in paraffin, sectioned, and stained using immunogold- and peroxidase-based techniques. Staining for both P450c17 and P450arom was intense in spherical 7- to 10-mm blastocysts, but was absent in earlier stage 2- to 4-mm blastocysts and less intense or absent in later stage 20-mm and filamentous embryos. Cytochrome P450c17 was limited to the trophoblast of all blastocysts expressing the enzyme, and in spherical 7- to 10-mm blastocysts, essentially all cells of the trophoblast layer stained positively for P450c17. However, as elongation became apparent in 10-mm blastocysts, the cells of the trophoblast became flattened, and the expression of P450c17 declined particularly in those trophoblast cells adjacent to the embryonic disc where mesoderm outgrowth was occurring. In fact, two distinct populations of trophoblast cells became obvious: one that maintained P450c17 expression, and one that did not. Moreover, those trophoblast cells expressing P450c17 were less flattened than neighboring cells in which P450c17 expression was absent. These two morphologically and functionally distinct trophoblastic cell populations were most obvious in areas furthest from the embryonic disc. Cytochrome P450arom was expressed in the trophoblast as well as the hypoblast under the embryonic disc. Neither P450c17 nor P450arom appeared to be expressed in the embryonic disc or the mesoderm of the expanding blastocyst. These functional and structural changes in the embryonic cell layers of the elongating conceptus may be associated with the transient synthesis and secretion of estrogen that occur at the time of maternal recognition of pregnancy in the pig.
Assuntos
Aromatase/análise , Blastocisto/citologia , Blastocisto/enzimologia , Embrião de Mamíferos/citologia , Embrião de Mamíferos/enzimologia , Esteroide 17-alfa-Hidroxilase/análise , Suínos/embriologia , Animais , Células Cultivadas , Feminino , Imuno-Histoquímica , Masculino , GravidezRESUMO
The aim of this investigation was to examine signaling between the placenta and uterus during pregnancy. To do this, we determined the tissue messenger RNA and protein levels of members of a glycopeptide hormone family known to stimulate the proliferation of uterine cells and related these levels to the growth of the uterus during pregnancy in the mouse. This hormone family is known as mitogen-regulated protein (MRP); alternatively proliferin (PLF). Three mrp/plf genes, plf1, mrp3 and mrp4, are expressed by the placenta with different developmental profiles. The major increase of about 4-fold in DNA content of the uterus occurs between days 9 and 14 when MRP/PLFs are present in the placenta. By contrast, the gestational changes in estradiol-17beta levels in placental and uterine tissues and in circulation do not correlate with the period of uterine growth. The previously reported mitogenic activity of the MRP/PLFs and their gestational profiles suggest that one or more of these proteins stimulates uterine proliferation during gestation. Evidence is also presented that expression of MRP3 and/or PLF1, but not MRP4, is negatively regulated by feedback from the uterus. Our results are consistent with the hypothesis that MRP/PLFs stimulate uterine proliferation in vivo and that a uterine factor shuts off PLF1 and/or MRP3 synthesis in the latter half of gestation.
Assuntos
Glicoproteínas/metabolismo , Placenta/metabolismo , Transdução de Sinais , Útero/metabolismo , Líquido Amniótico/química , Animais , Estradiol/sangue , Estradiol/metabolismo , Estradiol/farmacologia , Feminino , Regulação da Expressão Gênica , Idade Gestacional , Glicoproteínas/análise , Glicoproteínas/genética , Glicosilação , Peptídeos e Proteínas de Sinalização Intercelular , Camundongos , Camundongos Endogâmicos BALB C , Dados de Sequência Molecular , Ácido N-Acetilneuramínico/metabolismo , Placenta/química , Placenta/efeitos dos fármacos , Gravidez , Prolactina , RNA Mensageiro/análise , Distribuição Tecidual , Útero/crescimento & desenvolvimentoRESUMO
Since fetal serum Ig isotype profiles suggested that IgG and IgA could be of de novo origin, we studied their transcription and secretion. IgM transcripts were present at 50 days of gestation in major fetal lymphoid tissues, IgG and IgA transcription was pronounced at 60 days in fetal thymus and both transcription and secretion in this organ increased in late fetal life. The CDR3 spectratype of thymic IgG and IgA transcripts was as polyclonal as that of IgM already at 70 days in utero indicating a broad repertoire of switched B-cells. However, VDJs transcribed with the switched isotypes were not hypermutated as were those from immunized fetuses, indicating that switch recombination and somatic mutation are not coupled in utero in piglets. This finding and the fact that the oligoclonal IgA and IgM repertoires in a non-inductive site of the mucosal immune system (parotid gland) becomes polyclonal in piglets reared germ-free, suggest that initial expansion of switched B-cells in fetal and neonatal piglets is not driven by environmental antigen. Our findings collectively suggest that all IgA and IgM may result from de novo synthesis while some IgG probably results from selective transport. The latter is consistent with the gradual decline in serum IgG concentration in germ-free isolator piglets and the expression of FcRn in the porcine placenta.
Assuntos
Feto/imunologia , Imunoglobulinas/biossíntese , Recombinação Genética , Suínos/imunologia , Timo/metabolismo , Animais , Imunoglobulina G/biossíntese , Imunoglobulina G/genética , Imunoglobulina M/biossíntese , Imunoglobulina M/genética , Mutação , RNA Mensageiro/análise , Porco MiniaturaRESUMO
To date, no in vitro system has been devised to allow the study of both the functional and the structural regression of luteal cells in response to prostaglandin (PG) F2 alpha. This study describes the use of a novel intraluteal PGF2 alpha implant system that results in the death of individual corpora lutea (CL), while surrounding CL on the same ovary remain fully functional. By this technique, it was possible to study both the functional and the structural regression of individual CL in vivo, without the confounding effects resulting from the systemic injection of PGF2 alpha. Biochemical measurements of individual CL included progesterone concentration, protein kinase C activity, and diacylglycerol levels. Structural measurements included luteal weight and the protein:DNA ratio, which was used to estimate cell size. Further, the determination of large luteal cell size was accomplished directly via light microscopy. Nonpregnant gilts were injected with 5 mg of estradiol benzoate every 12 hr from 8:00 a.m. on Day 11 to 8:00 a.m. on Day 13 to prevent uterine PGF2 alpha secretion. At 7:00 a.m. on Day 13, CL on one ovary were selected at random to receive PGF2 alpha-implants (n = 4) or implant material only (n = 4), whereas the remaining CL on that ovary served as unimplanted controls. The other ovary was removed at the point, and the CL on that ovary served as 0-hr controls. Gilts were relaparotomized at 3, 6, 12, and 24 hr after CL implantation, the PGF2 alpha-implanted ovary was removed, and individual CL were evaluated. PGF2 alpha-implanted CL exhibited a decline (P < 0.05) in progesterone concentrations at 12 and 24 hr and a decline (P < 0.05) in weight at 24 hr when compared with control CL (implant-only, unimplanted, and 0-hr control CL). Furthermore, the protein:DNA ratio was reduced (P < 0.10) in the PGF2 alpha-treated CL at 12 and 24 hr. Moreover, this change in the protein:DNA ratio (cell size) was consistent with the reduced diameter (P < 0.05) of the large luteal cell in the PGF2 alpha-treated CL. Protein kinase C activity and diacylglycerol concentrations did not change (P > 0.10) and therefore appear to be unassociated with either functional or structural changes in the PGF2 alpha-treated CL. Contrary to in vitro culture studies, the results of our in vivo study demonstrate no clear role for protein kinase C in the PGF2 alpha-induced luteolytic process.(ABSTRACT TRUNCATED AT 400 WORDS)
Assuntos
Corpo Lúteo/efeitos dos fármacos , Dinoprosta/farmacologia , Luteólise/efeitos dos fármacos , Luteolíticos/farmacologia , Suínos/fisiologia , Animais , Corpo Lúteo/citologia , Corpo Lúteo/fisiologia , DNA/análise , Diglicerídeos/fisiologia , Dinoprosta/administração & dosagem , Implantes de Medicamento , Estradiol/farmacologia , Feminino , Luteólise/fisiologia , Luteolíticos/administração & dosagem , Tamanho do Órgão , Ovário/química , Ovário/efeitos dos fármacos , Ovário/fisiologia , Progesterona/farmacologia , Proteína Quinase C/fisiologia , Distribuição Aleatória , Fatores de TempoRESUMO
Eight multiparous beef cows were used to examine the effects of intrauterine infusion of catecholestradiol (4-hydroxylated estradiol) on development and function of the first corpus luteum after parturition. Calves were weaned on day 1 (day 0 = parturition) to initiate formation of a corpus luteum (CL) by approximately day 10 or 11. Before CL formation, on days 5 to 9, cows received twice daily infusions of catecholestradiol (4 micrograms; n = 4) or vehicle (n = 4) into the uterine horn opposite the previous pregnancy. Plasma progesterone during the first estrous cycle was elevated longer (P less than .001) and reached a higher (P less than .001) concentration in cows treated with catecholestradiol. The decline in progesterone was associated with an increase in plasma 13,14-dihydro, 15-keto-prostaglandin F2 alpha (PGFM) in all cows infused with catecholestradiol. In contrast, a rise in PGFM at the end of the first short cycle was detected in only one of four cows treated with vehicle. Furthermore, PGFM concentrations were linearly related (R2 = .870; P less than .001) to concentrations of progesterone. Estradiol-17 beta concentrations were not different during the infusion period, but after formation of the first CL, estradiol remained elevated (P less than .01) in cows that received vehicle. Results of this experiment suggest that exposure of postpartum beef cows to catecholestradiol extended luteal function in association with enhanced PGFM release.
Assuntos
Bovinos/fisiologia , Corpo Lúteo/crescimento & desenvolvimento , Dinoprosta/análogos & derivados , Estradiol/análogos & derivados , Estro/efeitos dos fármacos , Animais , Bovinos/sangue , Corpo Lúteo/fisiologia , Dinoprosta/sangue , Estradiol/farmacologia , Estrogênios de Catecol , Feminino , Progesterona/sangue , Fatores de Tempo , Útero/efeitos dos fármacosRESUMO
The influence of intrauterine administration of catecholestradiol (4-hydroxylated estradiol) on lifespan of the initial postpartum corpus luteum was evaluated in suckled beef cows. In experiment 1, postpartum cows (n = 23) were untreated (CONTROL) or received intrauterine infusions (0700 and 1700 hr) of either vehicle (SAL) or catecholestradiol (CATE; 4 micrograms) from day 15 to 22 (day 0 = parturition). Blood samples were collected three times weekly (day 15 to 100) and analyzed for progesterone. In experiment 2, cows received twice daily intrauterine infusions of either vehicle (n = 18), or catecholestradiol (n = 19), from day 25 +/- .5 to day 30 +/- .5. Following the final infusion, calves were temporarily weaned from all cows for 48 hr. At the end of the 48 hr weaning period, cows in each infusion group received either an i.m. injection of 1,000 IU hCG (SAL+hCG, n = 9; CATE+hCG, n = 9) or no further treatment (SAL, n = 9; CATE, n = 10). Blood samples were collected daily for 21 d following calf removal and 3 times weekly through 100 d postpartum. In both experiments, the initial postpartum elevation in peripheral progesterone concentrations was characterized as either a short (< 5 d) or extended (> 8 d) luteal phase. In experiment 1, postpartum anestrous interval (60 +/- 3.4 d) and incidence of short luteal phases (77%) were similar among CONTROL, SAL and CATE treatments. In experiment 2, luteal phases were induced within 10 d of onset of weaning in 90, 100, 56 and 60% of cows in SAL+hCG, CATE+hCG, SAL and CATE treatments, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Bovinos/fisiologia , Manutenção do Corpo Lúteo/efeitos dos fármacos , Estradiol/análogos & derivados , Estrogênios de Catecol/farmacologia , Ovulação/efeitos dos fármacos , Período Pós-Parto/efeitos dos fármacos , Análise de Variância , Animais , Gonadotropina Coriônica/farmacologia , Estradiol/administração & dosagem , Estradiol/farmacologia , Estrogênios de Catecol/administração & dosagem , Feminino , Fase Luteal/efeitos dos fármacos , Indução da Ovulação , Período Pós-Parto/fisiologia , Gravidez , Progesterona/sangue , Distribuição Aleatória , Fatores de Tempo , DesmameRESUMO
Reindeer are seasonally polyestrus, short day breeders, with estrous cycles of approximately 20 days in length. The objective of this study was to investigate the effects of reindeer bull exposure on the onset of the reindeer cow breeding season and to investigate whether cows with calving experience responded differently than cows with no previous reproductive experience. During year 1, blood samples were collected weekly beginning 14 July and continuing until 15 September (n = 8) or 30 September (n = 8) in order to determine the onset of the breeding season, based on ovarian function, with no bull present. Plasma was stored frozen for later assay of progesterone (P(4)) following the conclusion of sample collection. Eight randomly selected cows were allowed to breed with a bull during year 1. The mean onset of ovarian activity in the first year was 15 September (range: 8-29 September). The bull was removed from cows more than 2 months prior to the start of the experimental period during year 2 and housed at a separate facility approximately 2 km distant. Blood samples were collected during year 2 from 14 cows 3x weekly beginning on 11 August (day 1) and continuing until 29 September (day 50) and plasma was stored frozen for later assay of P(4) following the conclusion of sample collection. On day 6, cows were divided into two groups such that group 1 (early bull exposure; EBE), consisted of four cows that had calved the previous spring and four cows that had no reproductive experience (n = 8). Group 2 (late bull exposure; LBE), consisted of three cows that had calved the previous spring and three cows that had no reproductive experience (n = 6). EBE experienced bull introduction on day 13, 23 days earlier than the average onset of ovarian activity during year 1. LBE experienced bull introduction on day 46, 10 days later than the average onset of ovarian activity during year 1. Progesterone concentrations were analyzed by ANOVA procedures for repeated measures. Bull presence was not requisite for the onset of ovarian activity during either year. Previous reproductive status had no effect on the onset of ovarian activity within EBE (P = 0.61) or within LBE (P = 0.92). Time of bull introduction had a significant effect on the onset of ovarian activity when EBE was compared to LBE (P<0.001). The first sustained increase in mean P(4) concentration above 1 ng/ml occurred on day 25 in EBE reindeer and day 41 in LBE reindeer. EBE reindeer initiated ovarian activity 12 days after bull introduction while LBE reindeer initiated ovarian activity 5 days before bull introduction. All cows penned with the bull conceived during both breeding seasons, demonstrated by production of live calves during the subsequent spring. Cows bred during year 1 all calved within a 9 day-period. During year 2, EBE displayed a more synchronous calving period compared to LBE (P<0.05). Results indicate that bull management affects the onset of the breeding season in reindeer cows, regardless of previous reproductive experience.
Assuntos
Estro/fisiologia , Ovário/fisiologia , Paridade/fisiologia , Progesterona/sangue , Rena/fisiologia , Animais , Cruzamento , Feminino , Masculino , Gravidez , Rena/sangue , Estações do Ano , Comportamento Sexual Animal , Fatores de TempoRESUMO
Two experiments were conducted to examine whether the 40 or 50% decrease in systemic progesterone (P(4)) concentrations between Days 13 and 21 postmating in the pig results from decreased ovarian P(4) secretion or increased uptake of P(4) by the uterus. In Experiment I, five nonpregnant (NP) and four pregnant (P) gilts were sham-operated, and five NP gilts were hysterectomized (HYST) on Days 7 to 9 postestrus or postmating (first day of estrus or mating = Day 0). Femoral arterial blood was obtained once daily from Day 10 until the subsequent estrus (NP gilts) or Day 21 (P and HYST gilts). In Experiment II, blood was collected daily from both utero-ovarian veins of two NP and three P gilts from Days 11 to 18. Femoral arterial P(4) concentrations were similar for all gilts in Experiment I from Days 10 to 14. For NP gilts, femoral arterial P(4) declined (P < 0.01) after Day 14 to reach basal levels by Day 17. Progesterone in femoral arterial blood of P gilts declined (P < 0.01) from Days 13 to 16 and then remained constant through Day 21. Concentrations of P(4) in femoral arterial blood of HYST gilts remained constant from Days 13 to 21 and were greater (P < 0.01) than for P gilts from Days 15 to 21. In Experiment II, P(4) concentrations in utero-ovarian venous blood were similar until Day 14 between NP and P gilts. Utero-ovarian P(4) of NP gilts then declined (P < 0.01) to reach basal levels by Day 16. P(4) concentrations in utero-ovarian venous blood of P gilts increased (P < 0.05) for Days 14 to 18. These results demonstrate that ovarian P(4) secretion increases during early pregnancy in the pig. Further, the absence of a decline in P(4) concentrations in femoral arterial blood of HYST gilts suggests that the declining systemic P(4) levels observed during early pregnancy are a result of uterine uptake and(or) metabolism.