Do changes in Lactuca sativa metabolic performance, induced by mycorrhizal symbionts and leaf UV-B irradiation, play a role towards tolerance to a polyphagous insect pest?

The increased ultraviolet radiation (UV) due to the altered stratospheric ozone leads to multiple plant physiological and biochemical adaptations, likely affecting their interaction with other organisms, such as pests and pathogens. Arbuscular mycorrhizal fungi (AMF) and UV-B treatment can be used as eco-friendly techniques to protect crops from pests by activating plant mechanisms of resistance. In this study, we investigated plant (Lactuca sativa) response to UV-B exposure and Funneliformis mosseae (IMA1) inoculation as well as the role of a major insect pest, Spodoptera littoralis. Lettuce plants exposed to UV-B were heavier and taller than non-irradiated ones. A considerable enrichment in phenolic, flavonoid, anthocyanin, and carotenoid contents and antioxidant capacity, along with redder and more homogenous leaf color, were also observed in UV-B-treated but not in AMF-inoculated plants. Biometric and biochemical data did not differ between AMF and non-AMF plants. AMF-inoculated plants showed hyphae, arbuscules, vesicles, and spores in their roots. AMF colonization levels were not affected by UV-B irradiation. No changes in S. littoralis-feeding behavior towards treated and untreated plants were observed, suggesting the ability of this generalist herbivore to overcome the plant chemical defenses boosted by UV-B exposure. The results of this multi-factorial study shed light on how polyphagous insect pests can cope with multiple plant physiological and biochemical adaptations following biotic and abiotic preconditioning.

Combining roller crimpers and flaming for the termination of cover crops in herbicide-free no-till cropping systems.

The termination of cover crops in conventional no-till systems is mostly conducted mechanically in combination with herbicides. Combining flaming and roller crimpers could be a viable solution to avoid using herbicides for cover crop termination in farming systems where herbicides are banned, or at least to reduce their use in an integrated management approach. This research tested the effects of flaming used in combination with three different types of roller crimpers to terminate a fall-sown cover crop mixture of winter pea and barley. The cover crop termination rate was visually assessed in terms of percentage of green cover provided by cover crop plants at different intervals from the termination date, and estimated using a log-logistic non-linear regression model with four parameters. Machine performance data are also reported. The results show that, irrespective of the roller type, flaming significantly boosted the effect of the roller crimpers. In fact, an economic threshold for cover crop suppression of 85% was reached only when the rollers were used in combination with flaming. Nevertheless, none of the methods were able to reach the 100% of cover crop suppression. In some case, the combined use of flaming and roller crimpers allowed reaching the 90% of cover crop devitalisation, which happened six weeks after the termination date. More importantly, the use of flaming in combination with rollers shortened the time needed to achieve the estimated levels of devitalisation, compared with the rollers used alone. We conclude that flaming is an effective tool to increase the effectiveness of roller crimpers. Nevertheless, further research is needed to identify solutions to overcome the barrier of the high operational costs of flaming, which is constraining its wider adoption by farmers. Future studies could focus, for instance, on the development of a new prototype of combined machine for crimping and flaming the cover crops simultaneously, which could potentially reduce the operational costs.