Data-Driven Prediction of Molecular Biotransformations in Food Fermentation.

Fermentation products, together with food components, determine the sense, nutrition, and safety of fermented foods. Traditional methods of fermentation product identification are time-consuming and cumbersome, which cannot meet the increasing need for the identification of the extensive bioactive metabolites produced during food fermentation. Hence, we propose a data-driven integrated platform (FFExplorer, http://www.rxnfinder.org/ffexplorer/) based on machine learning and data on 2,192,862 microbial sequence-encoded enzymes for computational prediction of fermentation products. Using FFExplorer, we explained the mechanism behind the disappearance of spicy taste during pepper fermentation and evaluated the detoxification effects of microbial fermentation for common food contaminants. FFExplorer will provide a valuable reference for inferring bioactive "dark matter" in fermented foods and exploring the application potential of microorganisms.

Effects of spraying nano-materials on the absorption of metal(loid)s in cucumber.

This report investigates the spraying of nano-silica and fullerene on cucumber leaves to expose their ability to reduce the toxicity and uptake of metal(loid)s. Cucumber seedlings were randomly divided into six treatment groups: 10 mg/L nano-SiO2, 20 mg/L nano-SiO2, 10 mg/L Fullerene, 20 mg/L Fullerene, 5 mg/L Fullerene + 5 mg/L nano-SiO2, and 10 mg/L Fullerene + 10 mg/L nano-SiO2. Nano-silica-treated plants exhibited evidence of the potential mitigation of metal(loid)s poisoning. Specifically, results showed that 20 mg/L of nano-silica promoted Cd uptake by plants; comparatively, 10 mg/L of nano-silica did not significantly increase the silicon content in plants. Both low-concentration combined treatment and low-concentration fullerene groups inhibited metal(loid)s uptake by plants. Scanning electron microscopy (SEM) was then used to observe the surface morphology of cucumber leaves. Significant differences were observed on disease resistance in plants across the different nano-material conditions. Collectively, these findings suggest that both nano-silica materials and fullerene have the potential to control metal(loid)s toxicity in plants.