RESUMO
We examined the intracellular mechanisms for endothelin-1-induced positive and negative inotropic components that coexist in the mouse ventricular myocardium using isolated ventricular tissue and myocytes from 4-week-old mice. In the presence of SEA0400, a specific inhibitor of the Na+-Ca2+ exchanger, endothelin-1 produced positive inotropy. Endothelin-1, when applied to cardiomyocytes in the presence of SEA0400, did not change the peak amplitude of the Ca2+ transient but increased intracellular pH and Ca2+ sensitivity of contractile proteins. On the other hand, in the presence of dimethylamiloride (DMA), a specific inhibitor of the Na+-H+ exchanger, endothelin-1 produced negative inotropy. In cardiomyocytes, in the presence of DMA, endothelin-1 produced a decrease in peak amplitude of the Ca2+ transient. In the presence of both DMA and SEA0400, endothelin-1 produced neither positive nor negative inotropy. Positive inotropy was blocked by BQ-123 and negative inotropy by BQ-788. These results suggested that endothelin-1-induced positive inotropy is mediated by ET(A) receptors, activation of the Na+-H+ exchanger and an increase in intracellular pH and Ca2+ sensitivity and that the negative inotropy is mediated by ET(B) receptors, activation of the Na+-Ca2+ exchanger and decrease in Ca2+ transient amplitude.
Assuntos
Endotelina-1/fisiologia , Miócitos Cardíacos/fisiologia , Receptor de Endotelina A/fisiologia , Receptor de Endotelina B/fisiologia , Trocador de Sódio e Cálcio/fisiologia , Trocadores de Sódio-Hidrogênio/fisiologia , Amilorida/análogos & derivados , Amilorida/farmacologia , Análise de Variância , Compostos de Anilina/farmacologia , Animais , Cálcio/fisiologia , Cardiotônicos/farmacologia , Relação Dose-Resposta a Droga , Endotelina-1/farmacologia , Ventrículos do Coração/citologia , Concentração de Íons de Hidrogênio , Técnicas In Vitro , Camundongos , Camundongos Endogâmicos , Contração Miocárdica/efeitos dos fármacos , Miócitos Cardíacos/efeitos dos fármacos , Oligopeptídeos/farmacologia , Peptídeos Cíclicos/farmacologia , Éteres Fenílicos/farmacologia , Piperidinas/farmacologia , Trocador de Sódio e Cálcio/antagonistas & inibidores , Trocadores de Sódio-Hidrogênio/antagonistas & inibidoresRESUMO
Positive inotropy by sarcoplasmic/endoplasmic reticulum Ca(2+) pump inhibitors was found and its mechanisms were analyzed pharmacologically. Thapsigargin and cyclopiazonic acid produced positive inotropy in isolated mouse left atria. The responses were inhibited by pretreatment of the endocardial surface with Triton X-100 or by indomethacin, which suggests that the inotropic responses were mediated by prostaglandin(s) released from the endocardial endothelium as well as acetylcholine-induced positive inotropy. The thapsigargin- and acetylcholine-induced positive inotropy was significantly inhibited by Gd(3+), La(3+) and lavendustin A, a tyrosine kinase inhibitor, but not by Ni(2+) and LOE908, a non-selective cation channel inhibitor. Gd(3+) and lavendustin A had no effect on the exogenously applied PGF(2)alpha-induced positive inotropy. In addition, acetylcholine did not induce any positive inotropy when applied after the application of thapsigargin. These results strongly suggest that thapsigargin- as well as acetylcholine-induced prostaglandin release from endocardial endothelium is mediated by store-operated Ca(2+) entry through Gd(3+)-sensitive channels and activation of tyrosine kinase.