RESUMO
Organs cryopreserved by vitrification are exposed to the lowest possible concentration of cryoprotectants for the least time necessary to successfully avoid ice formation. Faster cooling and warming rates enable lower concentrations and perfusion times, reducing toxicity. Since warming rates necessary to avoid ice formation during recovery from vitrification are typically faster than cooling rates necessary for vitrification, warming speed is a major determining factor for successful vitrification. Dielectric warming uses an oscillating electric field to directly heat water and cryoprotectant molecules inside organs to achieve warming that's faster and more uniform than can be achieved by heat conduction from the organ surface. This work studied 27 MHz dielectric warming of rabbit kidneys perfused with M22 vitrification solution. The 27 MHz frequency was chosen because its long wavelength and penetration depth are suitable for human organs, because it had an anticipated favorable temperature of maximum dielectric absorption in M22, and because it's an allocated frequency for industrial and amateur use with inexpensive amplifiers available. Previously vitrified kidneys were warmed from -100 °C by placement in a 27 MHz electric field formed between parallel capacitor plates in a resonant circuit. Power was varied during warming to maintain constant electric field amplitude between the plates. Maximum power absorption occurred near -70 °C, with a peak warming rate near 150 °C/min in 50 mL total volume with approximately 500 W power. After some optimization, it was possible to warm â¼13 g vitrified kidneys with unprecedentedly little injury from medullary ice formation and a favorable serum creatinine trend after transplant. Distinct behaviors of power absorption and system tuning observed as a function of temperature during warming are promising for non-invasive thermometry and future automated control of the warming process at even faster rates with user-defined temperature dependence.
Assuntos
Criopreservação , Crioprotetores , Rim , Vitrificação , Animais , Coelhos , Criopreservação/métodos , Crioprotetores/química , Temperatura Alta , Preservação de Órgãos/métodos , Preservação de Órgãos/instrumentaçãoRESUMO
El ameloblastoma se origina de la lámina dentaria o de sus derivados, es un tumor localmente invasor, el más agresivo y frecuente de los tumores odontogénicos. Es de crecimiento lento que invade localmente, con una alta tendencia a la recidiva. Puede ser periférico o intraósea, que es el más frecuente. El periférico es menos agresivo e invasor y con una tendencia a la recidiva. Presenta un patrón histológico variado siendo los más comunes el folicular y el plexiforme. Las formas foliculares con más recidivantes que los plexiformes y las multioculares más que las uniloculares. Radiológicamente es una lesión radiolúcida, quística que se extiende de la región molar hasta la porción superior de la rama mandibular, con multilocularidad, variación en el tamaño de las loculaciones, expansión de la cortical, bordes bien definidos y resorción de las raíces dentales. Su incidencia alta de recidiva y lo complejo de las estructuras faciales que afectan hace que su abordaje terapeútico deba realizarse con base en un criterio médico multidisciplinario