Implantable Aptamer-Graphene Microtransistors for Real-Time Monitoring of Neurochemical Release in Vivo.

The real-time monitoring of neurochemical release in vivo plays a critical role in understanding the biochemical process of the complex nervous system. Current technologies for such applications, including microdialysis and fast-scan cyclic voltammetry, suffer from limited spatiotemporal resolution or poor selectivity. Here, we report a soft implantable aptamer-graphene microtransistor probe for real-time monitoring of neurochemical release. As a demonstration, we show the monitoring of dopamine with nearly cellular-scale spatial resolution, high selectivity (dopamine sensor >19-fold over norepinephrine), and picomolar sensitivity, simultaneously. Systematic benchtop evaluations, ex vivo experiments, and in vivo studies in mice models highlight the key features and demonstrate the capability of capturing the dopamine release dynamics evoked by pharmacological stimulation, suggesting the potential applications in basic neuroscience studies and studying neurological disease-related processes. The developed system can be easily adapted for monitoring other neurochemicals and drugs by simply replacing the aptamers functionalized on the graphene microtransistors.

First flush stormwater pollution in urban catchments: A review of its characterization and quantification towards optimization of control measures.

Identification, quantification, and control of First-Flush (FF) are considered extremely crucial in urban stormwater management. This paper reviews the methods for FF phenomenon identification, characteristics of pollutants flushes, technologies for FF pollution control, and the relationships among these factors. It further discusses FF quantification methods and optimization of control measures, aiming to reveal directions for future studies on FF management. Results showed that statistical analyses and Runoff Pollutographs Applying Curve (RPAC) fitting modelling of wash-off processes are the most applicable FF identification methods currently available. Furthermore, deep insights into the pollutant mass flushing of roof runoff may be a critical approach to characterizing FF stormwater. Finally, a novel strategy for FF control is established comprising multi-stage objectives, coupling LID/BMPs optimization schemes and Information Feedback (IF) mechanisms, aiming towards its application for the management of urban stormwater at the watershed scale.

Multiplexed Monitoring of Neurochemicals via Electrografting-Enabled Site-Selective Functionalization of Aptamers on Field-Effect Transistors.

Neurochemical corelease has received much attention in understanding brain activity and cognition. Despite many attempts, the multiplexed monitoring of coreleased neurochemicals with spatiotemporal precision and minimal crosstalk using existing methods remains challenging. Here, we report a soft neural probe for multiplexed neurochemical monitoring via the electrografting-assisted site-selective functionalization of aptamers on graphene field-effect transistors (G-FETs). The neural probes possess excellent flexibility, ultralight mass (28 mg), and a nearly cellular-scale dimension of 50 µm × 50 µm for each G-FET. As a demonstration, we show that G-FETs with electrochemically grafted molecular linkers (-COOH or -NH2) and specific aptamers can be used to monitor serotonin and dopamine with high sensitivity (limit of detection: 10 pM) and selectivity (dopamine sensor >22-fold over norepinephrine; serotonin sensor >17-fold over dopamine). In addition, we demonstrate the feasibility of the simultaneous monitoring of dopamine and serotonin in a single neural probe with minimal crosstalk and interferences in phosphate-buffered saline, artificial cerebrospinal fluid, and harvested mouse brain tissues. The stability studies show that multiplexed neural probes maintain the capability for simultaneously monitoring dopamine and serotonin with minimal crosstalk after incubating in rat cerebrospinal fluid for 96 h, although a reduced sensor response at high concentrations is observed. Ex vivo studies in harvested mice brains suggest potential applications in monitoring the evoked release of dopamine and serotonin. The developed multiplexed detection methodology can also be adapted for monitoring other neurochemicals, such as metabolites and neuropeptides, by simply replacing the aptamers functionalized on the G-FETs.

Amplification-Free Detection of SARS-CoV-2 and Respiratory Syncytial Virus Using CRISPR Cas13a and Graphene Field-Effect Transistors.

The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems have recently received notable attention for their applications in nucleic acid detection. Despite many attempts, the majority of current CRISPR-based biosensors in infectious respiratory disease diagnostic applications still require target preamplifications. This study reports a new biosensor for amplification-free nucleic acid detection via harnessing the trans-cleavage mechanism of Cas13a and ultrasensitive graphene field-effect transistors (gFETs). CRISPR Cas13a-gFET achieves the detection of SARS-CoV-2 and respiratory syncytial virus (RSV) genome down to 1 attomolar without target preamplifications. Additionally, we validate the detection performance using clinical SARS-CoV-2 samples, including those with low viral loads (Ct value >30). Overall, these findings establish our CRISPR Cas13a-gFET among the most sensitive amplification-free nucleic acid diagnostic platforms to date.

Health wearable devices for weight and BMI reduction in individuals with overweight/obesity and chronic comorbidities: systematic review and network meta-analysis.

OBJECTIVE: To analyse the comparative effectiveness of different health wearable-based physical activity (PA) promotion intervention strategies against each other and control for reducing body weight and body mass index (BMI) in individuals with overweight/obesity and chronic comorbidities. DESIGN: Systematic review and network meta-analysis (PROSPERO identifier: CRD42020158191). DATA SOURCES: We performed two independent searches from December 2019 to September 2020 in PubMed, MEDLINE, Scopus, Web of Science, Central Register of Controlled Trials, EMBASE and PsycINFO databases for articles published in English between 2007 and 2020. ELIGIBILITY CRITERIA FOR SELECTING STUDIES: Inclusion criteria were based on the PICOS framework. We included randomised controlled trials of health wearable-based interventions using two or more PA intervention arms/strategies and compared their effects on participants' body weight (kg) and BMI (kg/m2) with a control group. Data were analysed using a Bayesian network meta-analysis to directly and indirectly compare the effects of the six different intervention strategies (comparators). The six comparators were: (1) control group (ie, usual care, waitlist); (2) comparison group (ie, traditional, non-health wearable PA interventions); (3) commercial health wearable-only intervention (eg, Fitbit, Polar M400); (4) research grade health wearable-only intervention (ie, accelerometers or pedometers); (5) multicomponent commercial health wearable intervention (eg, Fitbit + nutrition counselling); and (6) multicomponent research grade health wearable intervention. The results were reported as standardised mean differences (SMDs) with associated 95% credible intervals (CrIs). RESULTS: From 641 screened records, 31 studies were included. For body weight reduction in individuals with overweight/obesity and chronic comorbidities, accelerometer/pedometer-only (SMD -4.44, 95% CrI -8.94 to 0.07) and commercial health wearable-only (SMD -2.76, 95% CrI -4.80 to -0.81) intervention strategies were the most effective compared with the three other treatments and control. For BMI reduction, multicomponent accelerometer/pedometer (SMD -3.43, 95% CrI -4.94 to -2.09) and commercial health wearable-only (SMD -1.99, 95% CrI -4.95 to 0.96) intervention strategies were the most effective compared with the other four conditions. CONCLUSION: Health wearable devices are effective intervention tools/strategies for reducing body weight and BMI in individuals with overweight/obesity and chronic comorbidities.

A Systematic Review of Active Video Games on Youth's Body Composition and Physical Activity.

In the past decade, active video games (AVGs) have started to find their way into communities, schools, and homes as a possible solution to promote physical activity (PA) and prevent childhood obesity. However, the evidence regarding its effectiveness on body composition and objectively measured PA in youth remains inconclusive. Thus, a systematic review on this topic is needed. This review synthesizes effects of AVGs on body composition and PA in youth. The initial search yielded 260 articles from 10 databases and 18 randomized-controlled trials were included after evaluating against eligibility and removing duplicates. Overall, AVGs showed positive effects in improving body composition and PA in overweight/obese youth as compared to the control conditions with only 2 studies displaying neutral effects on these outcomes as compared to traditional PA or comparison programs. For healthy youth, more than half of the studies (n = 10) demonstrated neutral effects of AVGs on these outcomes whereas only 6 yielded positive effects compared to the control conditions. Further, one study indicated that the control condition observed greater improvement in body composition compared with an AVG intervention in healthy youth. In summary, AVGs are deemed a promising addition to promote PA and health among overweight/obese youth with the goal of fighting childhood obesity.

The effects of the combined exercise intervention based on internet and social media software (CEIBISMS) on quality of life, muscle strength and cardiorespiratory capacity in Chinese postoperative breast cancer patients:a randomized controlled trial.

BACKGROUND: Breast cancer (BC) patients who undergo surgery followed by radiotherapy and chemotherapy have limitations on physical activity which will lead to a decreased quality of life and poor physical fitness level. The purpose of this study was to investigate the effects of the combined exercise intervention based on internet and social media software (CEIBISMS) on postoperative breast cancer patients by evaluating their quality of life, muscle strength and cardiorespiratory capacity. METHODS: This study was a randomized control trial with an intervention period of 12 weeks. Sixty participants (30 in each group, 42-60 years old, female) were recruited through an outpatient department. Procedure of exercise in the intervention group included: via phone step-recording app, ask the individuals to complete the target number of steps within a specified period of exercise, four times per week; face-to-face remote video instruction of individuals on muscle training, three times per week; via social media apps daily push common knowledge of physical exercise BC rehabilitation. The control group received traditional treatment and rehabilitation according to daily specifications of the hospital. The primary outcome was quality of life and the secondary outcomes were muscle strength and cardiorespiratory capacity. RESULTS: Experiments using a Short Form 36 showed that the CEIBISMS yielded significantly better results than traditional methods, in vitality (p = 0.009), mental health (p = 0.001) and reported health transition (p = 0.048) by week 12. The CEIBISMS resulted in significant improvement in the stand-up and sit-down chair test (p < 0.0001), arm lifting test (p = 0.017). CONCLUSION: The CEIBISMS offered rehabilitative effects in quality of life (QOL) and muscle strength of postoperative patients with breast cancer (BC) in China. TRIAL REGISTRATION: ChiCTR-IPR-17012368 . Trial registered on 14 August, 2017.

Bioinspired, Multiscale Reinforced Composites with Exceptionally High Strength and Toughness.

Nature's multiscale reinforcing mechanisms in fabricating composite armors, such as seashells, provide lessons for engineering materials design and manufacturing. However, it is still a challenge to simultaneously add both micro- and nanoreinforcements in a matrix material since nano-fillers tend to agglomerate, decreasing their reinforcing effects. In this study, we report a new type of micro/nano hybrid filler, synthesized by an unconventional cotton aided method, which has B4C microplatelet as the core and radially aligned B4C nanowires as the shell. To enhance the bonding between the B4C fillers and epoxy, the B4C micro/nano-fillers were coated with a layer of polyaniline (PANI). With a low concentration of the PANI functionalized B4C micro/nano-fillers (1 wt %), this B4C/epoxy composite exhibited an exceptional combination of mechanical properties in terms of elastic modulus (∼3.47 GPa), toughness (2026.3 kJ/m3), and fracture strain (>3.6%). An analytical mechanics model was established to show that such multiscale reinforcement design remarkably enhanced the load carrying efficiency of the B4C fillers, leading to the overall improved mechanical performance of the composites. This new design concept opens up a new path for developing lightweight, yet high-strength and tough materials with multiscale reinforcing configurations.

Retired Elite Athletes' Physical Activity, Physiological, and Psychosocial Outcomes During Single- and Double-Player Exergaming.

McDonough, DJ, Pope, ZC, Zeng, N, Lee, JE, and Gao, Z. Retired elite athletes' physical activity, physiological, and psychosocial outcomes during single- and double-player exergaming. J Strength Cond Res 33(12): 3220-3225, 2019-Elite athletes (i.e., athletes who play sport professionally) are a population who commonly exceed recommended physical activity (PA) guidelines and have higher health statuses compared with the general population. However, elite athletes transitioning into retirement often become physically inactive given they no longer require long hours of training and competition, and their physiological and psychosocial health suffers as a result. Therefore, this study's purpose was to examine differences in retired elite athletes' acute PA, physiological, and psychosocial outcomes during single- and double-player exergaming. Twenty retired Olympic athletes (18 females; (Equation is included in full-text article.)= 27.3 ± 4.3 years) participated in 2 separate 20-minute exergaming sessions (a): Xbox 360 Reflex Ridge single player and (b) Xbox 360 Reflex Ridge double player. Subjects' situational interest, enjoyment, and self-efficacy were examined using validated questionnaires; rating of perceived exertion (RPE) using the modified Borg RPE scale; and moderate-to-vigorous PA, steps, and energy expenditure tracked using ActiGraph GT3X+ accelerometers. Repeated-measures analysis of variances revealed no significant differences for any outcome between the 2 exercise sessions except for RPE (F (1, 38) = 4.6; p < 0.05; η = 0.11), which was higher in the single-player session compared with the double-player session (10.3 ± 2.3; 8.7 ± 1.6, respectively). Observations indicated double-player exergaming to be perceived as less intense than single-player exergaming despite similar PA and physiological outcomes, suggesting retired elite athletes may better adhere to exergaming in a double-player mode.

The Role of Youth Sports in Promoting Children's Physical Activity and Preventing Pediatric Obesity: A Systematic Review.

Youth sport participation plays an important role in promoting physical activity among children and may be a possible venue for the prevention of pediatric obesity. To design effective physical activity interventions, it is imperative to understand how different aspects of sport participation influence physical activity (PA). The purpose of this article is to present a comprehensive review of the impact of youth sport participation on children's PA and obesity status. A total of 44 studies published up to January 2014 concerning youth sport participation, PA, and obesity status were identified. Inclusion criteria were studies comparing PA levels of sport participants to nonparticipants or those comparing PA levels in different sport types and settings. Studies with the outcome variables of obesity status (e.g., body mass index, fat percentage, waist circumference) were also included. Participation in youth sport was positively associated with children's PA levels, and youth participating in sports were more likely to persist in their PA. However, the relationship between youth sport participation and obesity status was inconclusive. Educators and sports professionals should find ways to involve children in various sports settings and policies and help obese children engage more in sports.

Capillarity Composited Recycled Paper/Graphene Scaffold for Lithium-Sulfur Batteries with Enhanced Capacity and Extended Lifespan.

An effective strategy to tackle the twin crises of global deforestation and fossil fuel depletion is to recycle biomass materials for energy storage devices. This study reports a unique and innovative solution to capitalize on a currently overlooked resource to produce high-performance lithium-sulfur (Li-S) batteries from recycled paper. The recycled paper fibers are creatively composited with graphene oxide sheets via a capillary adsorption method. The recycled paper/graphene oxide hybrid is then converted to activated paper carbon/reduced graphene oxide (APC/graphene) scaffold for sulfur infiltration. The assembled Li-APC/graphene/S battery exhibits a superior lifespan of 620 cycles with an excellent capacity retention rate of 60.5%. An APC interlayer is sandwiched between the Li anode and the separator to suppress the degradation of Li anode by preventing the nonhomogeneous growth of mossy Li whiskers, stretching the battery lifespan up to 1000 cycles with a capacitance retention rate of 52.3%. The capillary adsorption method coupled with the porous carbonaceous anode interlayer configuration creates a new opportunity for the development of batteries derived from porous biomass materials.

The effects of active video games on patients' rehabilitative outcomes: A meta-analysis.

A meta-analysis on Active Video Games (AVG) as a rehabilitative tool does not appear to be available. This meta-analytic review synthesizes the effectiveness of AVGs on patients' rehabilitative outcomes. Ninety-eight published studies on AVGs and rehabilitation were obtained in late 2015 with 14 meeting the following inclusion criteria: 1) data-based English articles; 2) randomized-controlled trials investigating AVG's effect on rehabilitative outcome(s); and 3) ≥1 comparison present in each study. Data extraction for comparisons was completed for three age categories: 1) youth/young adults (5-25years-old); 2) middle-aged adults (40-65years-old); and 3) older adults (≥65years-old). Comprehensive Meta-Analysis software calculated effect size (ES; Hedge's g). Comparison group protocols often employed another non-AVG experimental treatment. Control group protocols implemented standard care. AVGs demonstrated a large positive effect on balance control over control among youth/young adults (ES=0.81, p<0.01). Further, AVGs resulted in small positive effects on middle-aged adults' balance control over control (ES=0.143, p=0.48) and comparison (ES=0.14, p=0.53), with similar results in older adults compared to control (ES=0.16, p=0.27). Notably, AVG's effect on balance control versus comparison among older adults was small yet negative (ES=-0.12, p=0.63). AVGs were also used to enhance general physical functioning (GPF) among middle-aged and older adults. Versus control and comparison, AVGs had no effect on middle-aged adults' GPF (ES=-0.054 and -0.046, respectively) or older adults' GPF (ES=0.04 and 0.002, respectively). Finally, AVGs had a moderate effect on older adults' falls efficacy versus control (ES=0.61, p<0.05). Findings favor AVGs for youth/young adult balance control rehabilitation and falls efficacy promotion in older adults.

Investigating elementary school children's daily physical activity and sedentary behaviours during weekdays.

The purpose of the study was to quantify the contributions of physical education, exergaming (active video games that also are a type of exercise), recess, lunch break and after-school time segments to children's daily physical activity and sedentary behaviours. Participants were 138 second and third graders (71 girls) who attended 20-min recess and 75-min lunch time daily, 25-min regular physical education or exergaming-based classes being alternated daily. The after-school period was defined as 3:20-10:00pm. Physical activity was assessed via accelerometry and the dependent variables were children's time spent in moderate-to-vigorous physical activity (MVPA), light physical activity and sedentary behaviour. Children's percentages of time spent in MVPA (P < .001; except for the difference between exergaming and lunch break: P = .63), light physical activity (P < .001) and sedentary behaviour (P < .001) differed significantly across the time segments (i.e., physical education/exergaming, recess, lunch break and after-school). Additionally, children accumulated significantly more MVPA (t = 10.22, P < .001) but less light physical activity (t = -3.17, P = .002) and sedentary behaviour (t = -3.91, P < .001) in physical education than in exergaming. Overall, physical education was more effective in generating MVPA than other segments over the school day. The after-school segment holds potential as an avenue for promoting children's MVPA, as this long period could be better utilised to organise structured physical activity.

Cotton-Textile-Enabled, Flexible Lithium-Ion Batteries with Enhanced Capacity and Extended Lifespan.

Activated cotton textile (ACT) with porous tubular fibers embedded with NiS2 nanobowls and wrapped with conductive graphene sheets (ACT/NiS2-graphene) was fabricated by a simple two-step heat treatment method. When used as a binder-free electrode, the ACT/NiS2-graphene electrode exhibited an exceptional electrochemical performance including ultrahigh initial discharge capacity (∼1710 mAh g(-1) at 0.01 C), magnificent rate performance (the discharge capacitance retained at ∼645 mAh g(-1) at 1 C after 100 cycles) and excellent cyclic stability (the discharge capacitance recovered to ∼1016 mAh g(-1) at 0.1 C after 400 cycles).

The contributing role of physical education in youth's daily physical activity and sedentary behavior.

BACKGROUND: School physical education (PE) is considered as an effective channel for youth to accumulate moderate-to-vigorous physical activity (MVPA) and reduce sedentary time. The purpose of this study was to determine the contributing role of PE in daily MVPA and sedentary time among youth. METHODS: The study recruited 67 sixth grade children (29 boys; Mean age = 11.75) from two suburban schools at a U.S. Midwest state, 48 of whom contributed ≥10 hours of physical activity (PA) data per day were included for analysis. An objective monitoring tool (i.e., Sensewear armband monitor) was used to capture the participants' MVPA and sedentary time for 7-14 days. Pearson product-moment correlation analysis (r), multi-level regression analyses, and analysis of variance were conducted for data analysis. RESULTS: MVPA and sedentary time in PE showed significant positive associations with daily MVPA and sedentary time, respectively (r = 0.35, p < 0.01; r = 0.55, p < 0.01). Regression analyses revealed that one minute increase in MVPA and sedentary behavior in PE was associated with 2.04 minutes and 5.30 minutes increases in daily MVPA and sedentary behavior, respectively, after controlling for sex and BMI. The participants demonstrated a significantly higher level of MVPA (p = .05) but similar sedentary time (p = 0.61) on PE days than on non-PE days. Boys had significantly more daily MVPA (p < .01) and less sedentary time (p < .01) than girls; while higher BMI was associated with more sedentary time (p < .01). CONCLUSIONS: PE displayed a positive contribution to increasing daily MVPA and decreasing daily sedentary time among youth. Active participation in PE classes increases the chance to be more active and less sedentary beyond PE among youth.

Dynamic relationships between motor skill competence and health-related fitness in youth.

This cross-sectional study examined associations among motor skill competence (MSC) and health-related fitness (HRF) in youth. A convenient sample of 253 boys and 203 girls (aged 4-13 years) participated in the study. Associations among measures of MSC (throwing and kicking speed and standing long jump distance) and a composite measure of HRF (push-ups, curl-ups, grip strength and PACER test) across five age groups (4-5, 6-7, 8-9, 10-11 and 12-13 yrs.) were assessed using hierarchical regression modeling. When including all children, throwing and jumping were significantly associated with the composite HRF factor for both boys and girls (throw, t = 5.33; jump, t = 4.49) beyond the significant age effect (t = 4.98) with kicking approaching significance (t = 1.73, p = .08). Associations between throwing and kicking speed and HRF appeared to increase from early to middle to late childhood age ranges. Associations between jumping and HRF were variable across age groups. These results support the notion that the relationship between MSC and HRF performance are dynamic and may change across childhood. These data suggest that the development of object control skills in childhood may be important for the development and maintenance of HRF across childhood and into adolescence.

Examining elementary school children's level of enjoyment of traditional tag games vs. interactive dance games.

Enjoyment has been implicated as a determinant of physical activity among children and adolescents. However, the effect of different sport activities on children's enjoyment remains largely unexplored. This study examined whether children's enjoyment in physical education (PE) varied as a function of learning activities. Participants were 210 third- through sixth-grade children who had a 30 min PE class every week. Participants responded to a standardized self-report enjoyment survey measuring their enjoyment level in a PE class during which they participated in tag games. Students completed the same questionnaire when involved in interactive dance games in PE. The results revealed that children reported significantly higher scores in enjoyment toward interactive dance games than they did toward traditional games (p < .01). Also, girls exhibited higher enjoyment toward interactive dance games than boys did (p < .05). However, no gender difference emerged on enjoyment toward traditional games. In conclusion, it is practical and meaningful to integrate interactive dance games into PE.

Physical Impact of Traditional and Virtual Physical Exercise Programs on Health Outcomes among Corporate Employees.

Background: Technology-based exercise programs have the potential to reduce chronic disease prevalence and obesity-related risks. This research focuses on the impact of both traditional and virtual exercise programs on the health metrics of corporate employees. Methods: The study involved 30 corporate employees (16 females, average age ± SD = 37.8 ± 8.8 years) from Nigeria, who were divided into two experimental groups (one experiencing traditional exercises with an on-site trainer, and the other participating in live, virtual classes via Zoom) and a control group continuing usual practices. The 6-week intervention measured several health outcomes, including resting heart rate, blood pressure (both diastolic and systolic), body mass index (BMI), waist circumference, and waist-hip ratio, both before and after the intervention. Results: One-way analysis of covariance yielded significantly greater decreases in the diastolic blood pressure, F(2,27) = 3.7, p < 0.04; systolic blood pressure F(2,27) = 3.5, p < 0.04, body mass index, F(2,26) = 7.8, p < 0.01, and waist circumference, F(2,27) = 39.1, p < 0.01. Conclusions: The study concludes that virtual-based exercise programs are as effective as traditional ones. Offering a virtual exercise option provides flexibility, potentially increasing adherence to exercise routines among corporate workers.

Identity-Guided Collaborative Learning for Cloth-Changing Person Reidentification.

Cloth-changing person reidentification (ReID) is a newly emerging research topic aimed at addressing the issues of large feature variations due to cloth-changing and pedestrian view/pose changes. Although significant progress has been achieved by introducing extra information (e.g., human contour sketching information, human body keypoints, and 3D human information), cloth-changing person ReID remains challenging because pedestrian appearance representations can change at any time. Moreover, human semantic information and pedestrian identity information are not fully explored. To solve these issues, we propose a novel identity-guided collaborative learning scheme (IGCL) for cloth-changing person ReID, where the human semantic is effectively utilized and the identity is unchangeable to guide collaborative learning. First, we design a novel clothing attention degradation stream to reasonably reduce the interference caused by clothing information where clothing attention and mid-level collaborative learning are employed. Second, we propose a human semantic attention and body jigsaw stream to highlight the human semantic information and simulate different poses of the same identity. In this way, the extraction features not only focus on human semantic information that is unrelated to the background but are also suitable for pedestrian pose variations. Moreover, a pedestrian identity enhancement stream is proposed to enhance the identity importance and extract more favorable identity robust features. Most importantly, all these streams are jointly explored in an end-to-end unified framework, and the identity is utilized to guide the optimization. Extensive experiments on six public clothing person ReID datasets (LaST, LTCC, PRCC, NKUP, Celeb-reID-light, and VC-Clothes) demonstrate the superiority of the IGCL method. It outperforms existing methods on multiple datasets, and the extracted features have stronger representation and discrimination ability and are weakly correlated with clothing.

[Evolution of Water Quality and Characterization of Microbial Populations in Different Storage Forms of Roof Rainwater].

The safety and security of stored rainwater quality is the key to improve the efficiency of rainwater resources storage, and roof rainwater is the best scenario for rainwater storage and utilization. Through long-term monitoring of the evolution of water quality during the roof rainwater storage process, different storage materials （PE and glass） and different DO regulation modes （sealing and aeration） were constructed, and 16S rRNA microbial diversity sequencing and environmental factor correlation methods were used to characterize the changes in water quality under microbial metabolism during the rainwater storage process, as well as the potential risks of utilization and health. The results showed that the degradation of COD occurred mainly in the first 10 days of the storage process, and the nutrients were transformed mainly by microbial metabolism. There were differences in the characteristics of water quality changes under different water storage conditions, with traditional PE materials promoting the propagation of some pathogenic Xanthobacter, Alternaria, Stachybotrys, and Cladosporium, which were negatively correlated with DO and pH. Aeration was beneficial in reducing the abundance of bacteria and fungi, whereas the sealed water storage method was beneficial in inhibiting the growth of pathogenic bacteria such as Legionella.