[Leukocyte stimulation by DNA fragments shored up protamine in cyclophosphamide-induced leukopoiesis in mice].

Our study showed that protamine (80% w/w to DNA) effectively protected its molecules from degradation by native nucleases of the mammalian blood serum. Exogenous DNA bound to protamine effectively stimulated restoration of cyclophosphamide-induced leukopoiesis in mice. It is suggested that the phenomenon was due to repair processes taking place in hemopoietic stem cells damaged by a cross-linking cytostatic drug such as cyclophosphamide. DNA dosage may be reduced and the original DNA fragment size maintained by DNA binding to protamine. As a result, it might involve longer DNA fragments into repair processes of homologous recombination and eventually increase the cell's chances of getting rid of extensive damage.

[Genetic structure of the populations of native inhabitants of the northeastern USSR. IV. The Koryaks of Kamchatka]. / Geneticheskaia struktura populiatsii korennykh zhitelei Severo-Vostoka SSSR. Soobshchenie IV. Koriaki Kamchatki.

Genetic structure of four Kamchatka subpopulations (675 individuals) was estimated for 25 erytrocyte and serum systems, some blood groups and for taste sensitivity to PTC. 23 of 38 loci examined are completely monomorphic. These are: AK, Ca-1, Cat, Dia, Est1-4, GOT, G-6-PD, LDH A and B, MDH, PGM2, SoD, Hb alpha and beta, ChE1, Lap, Alb, Cp, Tf, Rh. Following allele frequencies were found for polymorphic loci: AcPA = = 0.616; AcPB = 0.383; AcPC = 0.0015; EsD1 = 0.882; GLO - I1 = 0.156; GPT1 = 0.611; PGDA = = 0.959; PGM1(1) = 0.953; ChE2+ = 0.039; Gc1 = 0.888; Hp1 = 0.173; r(0) = 0.620; P(A) = 0.201; q(B) = 0.179; le = 0.192; M = 0.397; P1+ = 0.585; t = 0.371. According to monomorphic and polymorphic loci set, Kamchatka Koryaks are rather similar to other ethnic North-East Asiatic groups, being the most approximate to Reindeer Chuckchies and the most remote from Alaskan and Asiatic Eskimos. In other words, the extent of genetic differences between Kamchatka Koryaks and North-East populations corresponds to the geographic distribution and the degree of ecological differences in these populations. Analysis of interpopulation heterogeneity permitted to reveal the extent of contribution of individual loci to "differentiation" of North-East ethnic groups. The possible influence of ecological factors on interpopulation and intersubpopulation heterogeneity of the loci analysed is discussed.

[Frequency of the HLA-DP genes and the antigens of the HLA-A, -B, -Cw, and -DR loci in Tuvinians]. / Chastota genov HLA-DP i antigenov lokusov HLA-A, -B, -Cw. -DR u tuvintsev.

The results of DNA typing of the HLA-DPB1 gene among Tuvinians are described. The allele 0201 was found to be the most frequent (26.66%); then, in order of decreasing frequency, followed the alleles 0401 (26.42%), 0402 (13.99%), 0301 (8.55%), 0501 (7.77%), 0901 (5.16%), and 1301 (2.07%). The frequency of the allele 0501 in Tuvinians was significantly lower than that in the published data on allele distributions characteristic of two other Mongoloid populations, the Manchu and Japanese. The results of the serological typing of the loci HLA-A, -B, and -Cw of class I in the Tuva population were compared to the corresponding published data on ten Mongoloid populations of Central and Eastern Asia: Uigurs, Kazakhs, Mongols, Manchu, Tibetans, the Chinese of the northern and southern regions of China, Thais (inhabitants of Northwestern Indochina), Koreans, and Japanese. In Tuvinians, the most frequent antigens were HLA-A2, -A9, -B0, -B35, -B17, -Cw4, and -DR5. In the inhabitants of Tuva, the frequency of the antigens HLA-A1, -A9, -B17, -Cw2, and -Cw4 was significantly higher than in other Mongoloids, while that of the antigens HLA-A26 and -B22 was lower. This difference was greatest in the case of antigen HLA-B40: it a frequency of 43.4% was detected in it, while in individuals of Mongolian origin, the frequency of this antigen did not exceed 22.5%. Indices of genetic similarity (IGSs), calculated from the gene frequencies of the loci HLA-A, -B, and -Cw, suggested that the Tuvinians were most closely related to Kazakhs (IGS = 0.73), and least related to the Japanese and Uigurs (IGS = 0.66). Our data suggest that Tuvinians significantly differ from the compared populations of Central and Eastern Asia.

[Frequency distribution of HLA phenotypes, haplotypes and alleles in ethnic groups of Taimyr and Chukotka]. / Rasprostranenie chastot fenotipov, gaplotipov, allelei HLA v étnicheskikh gruppakh Taimyra i Chukotki.

HLA typing data of two little populations living in Tajmyr peninsula (Dolgans and Nganasans) and Chukotka peninsula (Chukchas and Chuvantses) were presented. Our data were compared with the International standard distribution of class I HLA antigens in orients and its distribution in orients who live in the Asian part of Russia. Indexes of genetic distribution and genetic likeness were calculated.

[Comparative analysis of indicators of the immune status of population of the Eastern regions of the USSR]. / Sravnitel'nyi analiz pokazatelei immunnogo statusa naseleniia vostochnykh regionov SSSR.

Altogether 1,500 healthy residents of seven cities situated in the Asian part of the USSR were examined. In Novosibirsk, Tomsk, Tyumen, Norilsk, Magadan, Yakutsk and Ussuriisk, the people were examined for the blood levels of T and B lymphocytes, the ratio of regulatory subclasses of T lymphocytes, the concentration of IgG, IgM and IgA, and for the content of immune complexes. Analysis was made of the general and regional regularities in changes seen in the immune system depending on climatic and geographic factors. Parameters of the populations similarity in the regions with different environmental conditions were delineated.

[Functional asymmetry of the brain hemispheres: the role in pathogenesis of ectromelia and melanoma in (CBAxC57BL/6)F1 mice].

The object of the research was to identify role of functional nervous asymmetry in formation of pathology in experimental animals. The role of functional asymmetry of the brain hemispheres in development of ectromelia and melanoma at (CBAxC57BL/6)F1 mice was investigated. It has been established, that mice with domination of motor functions in the left hemisphere ("right-handed") are more resistant against infection with ectromelia virus, and melanoma B-16 grows more slowly in these mice as compated with animals with domination of motor functions of the right hemisphere ("left-handed"). Hence, for the first time the role of functional nervous asymmetry in pathogenesis of ectromelia and melanoma (B-16) in mice (CBAxC57BL/6)F1 is established.