A solid-state 14N magic-angle spinning NMR study of some amino acids.

Experimental strategies for the acquisition of high-quality 14N magic-angle spinning (MAS) NMR spectra of the simple amino acids, which exhibit 14N quadrupole coupling constants (C(Q)) on the order of 1.2 MHz, are devised. These are the first useful 14N MAS spectra reported for nitrogen compounds having a C(Q)(14N) value in excess of 1 MHz. The complete manifolds of spinning sidebands (ssbs), i.e., about 300 ssbs for a spinning frequency of 6.0 kHz, have been observed in the 14N MAS NMR spectra of a series of amino acids. In their crystal structure these amino acids all exhibit the zwitterionic form and thus the 14N MAS NMR spectra represent those of a rotating -NH(3)(+) group and not of an amino (-NH(2)) group. Computer simulations combined with fitting of simulated to the experimental ssb intensities result in the determination of precise values for the 14N quadrupole coupling (C(Q)) and its associated asymmetry parameter (eta(Q)) for the nitrogen sites in these molecules. For some of the amino acids the 14N MAS NMR spectra exhibit overlap between the manifolds of ssbs from two different nitrogen sites in accordance with their crystal structures. Computer analysis of these spectra results in two different sets of (C(Q), eta(Q)) values which mainly differ in the magnitudes for eta(Q).

Unusual observation of nitrogen chemical shift anisotropies in tetraalkylammonium halides by 14N MAS NMR spectroscopy.

14N Magic-angle spinning (MAS) NMR spectra for a number of polycrystalline, symmetrical tetraalkylammonium halides with short alkyl chains (C2H(5)- to n-C4H(9)-) have been recorded following a careful setup of the experimental conditions. Analysis of the spectra demonstrates the presence of 14N chemical shift anisotropies (CSAs) on the order of |delta sigma| = 10-30 ppm along with 14N quadrupole coupling constants in the range of 10-70 kHz. The magnitude and sign of the CSAs determined from 14N MAS NMR are confirmed by recording and analysis of the corresponding slow-speed spinning (500-650 Hz) 15N CP/MAS NMR spectra. Most interestingly, it is observed experimentally and demonstrated theoretically and by simulations, that these CSAs are reflected in the spinning sideband (ssb) intensities of the 14N MAS spectra at much higher spinning speeds than can be applied to retrieve the corresponding 15N CSAs from the ssb pattern in the 15N CP/MAS spectra.