RESUMO
BACKGROUND: Numerous studies indicate mineral deficiencies in people on a gluten-free (GF) diet. These deficiencies may indicate that GF products are a less valuable source of minerals than gluten-containing products. In the study, the nutritional quality of 50 GF products is discussed taking into account the nutritional requirements for minerals expressed as percentage of recommended daily allowance (%RDA) or percentage of adequate intake (%AI) for a model celiac patient. Elements analyzed were calcium, potassium, magnesium, sodium, copper, iron, manganese, and zinc. Analysis of %RDA or %AI was performed using principal component analysis (PCA) and hierarchical cluster analysis (HCA). RESULTS: Using PCA, the differentiation between products based on rice, corn, potato, GF wheat starch and based on buckwheat, chickpea, millet, oats, amaranth, teff, quinoa, chestnut, and acorn was possible. In the HCA, four clusters were created. The main criterion determining the adherence of the sample to the cluster was the content of all minerals included to HCA (K, Mg, Cu, Fe, Mn); however, only the Mn content differentiated four formed groups. CONCLUSION: GF products made of buckwheat, chickpea, millet, oats, amaranth, teff, quinoa, chestnut, and acorn are better source of minerals than based on other GF raw materials, what was confirmed by PCA and HCA. © 2017 Society of Chemical Industry.
Assuntos
Dieta Livre de Glúten/normas , Grão Comestível/química , Minerais/análise , Avena/química , Chenopodium quinoa/química , Fagopyrum/química , Glutens/análise , Humanos , Milhetes/química , Valor Nutritivo , Recomendações Nutricionais , Zea mays/químicaRESUMO
FT-NIR spectroscopy and color measurements were applied to evaluate the influence of packaging on cosmetic cream stored at room and refrigerated conditions. Commercial cosmetic cream was stored for 2 and 4 mo in five containers intended for cosmetics: aluminum jar with polypropylene thermos (Al/PP), acrylic jar with polypropylene thermos (Ac/PP), glass (G) jar, polypropylene (PP) jar, and styrene acrylonitrile (SAN) jars. Principal component analysis (PCA) of the FT-NIR spectra showed the effect of time on tested samples stored in all applied packagings; separate groups were formed by fresh samples and samples stored for 2 and 4 mo. The changes in samples stored in the cold for 2 mo were similar for all applied packagings as compared with fresh samples, although samples stored in SAN jars formed a separate group. After 4 mo, a separate group was formed by samples stored in G jars. For samples stored at room temperature, the influence of packaging material on cosmetic emulsion was clearly visible; four separate groups (Al/PP with PP, Ac/PP, G, and SAN) were created by samples stored for 4 mo. Using partial least squares (PLS1) regression, it was found that the FT-NIR spectra of tested samples correlated with their lightness L*, a* parameter, and total color difference ΔE * ab . FT-NIR spectroscopy is a rapid technique which could be useful to make the best choice of packaging for cosmetics protecting the original quality of products during long-term storage.
Assuntos
Cosméticos/química , Emulsões/química , Embalagem de Produtos , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , Espectroscopia de Luz Próxima ao Infravermelho/métodos , Acetonitrilas/química , Análise dos Mínimos Quadrados , Polipropilenos/química , Estireno/química , Temperatura , Fatores de TempoRESUMO
BACKGROUND: Enzymatic browning of fruits and vegetables and their products is an important factor worsening their quality. The influence of five green tea extracts at the concentrations of 1 g L-1 , 2 g L-1 and 3 g L-1 on polyphenol oxidase (PPO) activity in fresh cloudy apple juice was investigated. Moreover, PPO inhibition by tea extract and colour stability of juice during short-time refrigerated storage was studied. The changes of juice colour during storage was expressed as the total colour differences (ΔE*), browning index (BI), yellowness index (YI), and the absorbance at 420 nm (A420 ). RESULTS: All extracts inhibited PPO activity in fresh apple juice in concentration-dependent manner. PPO activity in pure apple juice decreased by 7% after 48 h, whereas PPO activity in samples with 1 g L-1 , 2 g L-1 and 3 g L-1 tea extract decreased by 53%, 74%, and 96%, respectively. Browning of apple juice during storage decreased with increased concentration of green tea extract. After 48 h, extract at 1 g L-1 , 2 g L-1 and 3 g L-1 inhibited browning of juice expressed as BI by 48%, 60%, and 86%, respectively, comparing to pure apple juice. CONCLUSION: Green tea extract may be an effective anti-browning agent for short-time stored cloudy apple juices. © 2016 Society of Chemical Industry.
Assuntos
Catecol Oxidase/antagonistas & inibidores , Manipulação de Alimentos/métodos , Sucos de Frutas e Vegetais/análise , Malus/química , Extratos Vegetais , Chá/química , Antioxidantes/análise , Cor , Armazenamento de Alimentos , Malus/enzimologiaRESUMO
Cosmetic emulsions containing plant extracts should be tested in a range of temperatures from 5°C to 40°C to be sure that they will be stable during general use by consumers and that plant extracts used as antioxidants do not accelerate oxidative degradation of their oil base. The oxidative stability of argan oil-in-water emulsions containing 1% and 5% commercial acerola, willow, and rose extracts [or 0.01% butylhydroxytoluene (BHT)], stored at 5°C and 20°C for 6 months and at 40°C for 4 weeks, was monitored by the determination of peroxide content. The antioxidant or pro-oxidant activities of extracts or BHT in emulsions were expressed as the protection factor (PF) and inhibition of peroxide formation (Ip). At the end of storage, 5% willow, 0.01% BHT, 1% willow, and 5% acerola were the most protective for emulsions stored at 5°C. At 20°C, the most effective was 0.01% BHT, 5% rose, and 5% acerola. At 40°C, inhibition of peroxide formation calculated for 1% rose, 5% acerola, and 0.01 % BHT was similar. Altogether, the results show that some plant extracts, depending on storage conditions, may act as pro-oxidants, whereas the others can be applied as natural antioxidants instead of synthetic BHT.
Assuntos
Antioxidantes/química , Cosméticos/química , Oxidantes/química , Extratos Vegetais/química , Óleos de Plantas/química , Emulsões , Malpighiaceae/química , Rosa/química , Salix/química , Água/químicaRESUMO
The application of flaxseed extracts as food ingredients is a subject of interest to food technologists and nutritionists. Therefore, the influence of the extraction method on the content and composition of beneficial compounds as well as anti-nutrients is important. In the study, the effects of two solvent extraction methods, aqueous and 60 % ethanolic, on phenolic and cyanogenic glucoside profiles of flaxseed extract were determined and compared. The impact of extracted phenolic compounds on the antioxidant capacity of the extracts was also investigated. Defatted meals from brown and golden flax varieties were used as extraction material. The ethanolic extraction was more selective for phenolics (100.8-131.7 mg g-1) than the aqueous one (11.5-15.7 mg g-1). However, the contribution of particular phenolic compounds to total phenolics was much more dependent on flax variety than extraction method. A strong relationship was observed between both radical scavenging and ferric reducing activity and the content of phenolics (particularly secoisolariciresinol diglucoside). The correlation between extract chelating ability and phenolics was moderate suggesting that other flaxseed compounds are involved in this activity. The extraction method strongly affected cyanogenic glucoside content of flaxseed extracts; the aqueous extraction caused 96 % reduction in cyanogenic glucoside content (0.56-0.62 mmol g-1) when compared to the content in defatted meal (9.1-11.6 mmol g-1). On the contrary, ethanolic extraction resulted in the high cyanogenic glucoside content in the extracts (71-89 mmol g-1). The results reveals that ethanolic extraction gives extracts rich in antioxidant lignans; aqueous extracts have lower antioxidant activity than ethanolic but cyanogenic glucosides are significantly reduced.
RESUMO
The nutritional value of gluten-free products is the subject of interest for food technologists and nutritionists, as the only effective treatment for celiac disease is a lifelong gluten-free diet. As selenium deficiencies in celiac disease are observed, the aim of the study was to determine the selenium content in 27 grain gluten-free products available on the European Union (EU) market. Moreover, selenium content in products based on popular gluten-free cereals like corn, rice, and buckwheat and in relatively new or less popular products based on oat, amaranth, teff, and quinoa was compared. Selenium content in the tested products ranged from 0.9 to 24.5 µg/100 g. The average content of selenium in products based on popular gluten-free cereals was 2.8 µg/100 g and in products based on oat, amaranth, teff, and quinoa was 10.8 µg/100 g. It indicates that products based on less popular grains, especially on oat, should be more frequently chosen as a source of selenium by people on gluten-free diet than traditionally consumed gluten-free grains.
Assuntos
Grão Comestível/química , Selênio/análise , Chenopodium quinoa/química , Fagopyrum/química , Análise de Alimentos , Tecnologia de Alimentos , Glutens/análise , Valor NutritivoRESUMO
The aim of this paper was to evaluate the influence of different indigenous lactic acid bacteria isolates - as a single culture or bacterial consortium - on the functional and physicochemical properties of fermented curly kale juice. All tested variants exhibited good growth parameters, manifested by efficient pH lowering, increases in acidity, and fructose and glucose metabolism, as well as a significant inhibition of pathogens. A slight increase in total phenolic content was observed, while antioxidant activity remained unchanged. L. sakei and MIX A were associated with an increase in riboflavin and pyridoxine content, while L. plantarum only contributed to an increase in vitamin B6 content. Bioconversion of individual phenolic compounds, carotenoids, and glucosinolates strongly depended on the strain-specific metabolism. In the process, the levels of ferulic acid and other hydroxycinnamic acids were maintained, while the content of 9-cis lutein increased. Considering presented results and our previous research regarding probiotic features of LAB strains, among tested starter cultures - L. plantarum seemed to possess the best characteristics as a potential starter culture for controlled fermentation of curly kale juice.
Assuntos
Brassica , Lactobacillales , Probióticos , Fermentação , Microbiologia de AlimentosRESUMO
The main purpose of the study was to investigate the quality of fulvic acid-based food products. The concentrations of Ca, K, Mg, Na, Cu, Fe, Mn, and Zn, and antioxidant capacities of fulvic acid concentrates and ready-to drink beverages available on the global market were determined. The concentrations of minerals were determined using microwave plasma-atomic emission spectrometry. Antioxidant capacity was expressed as total polyphenol (TP) and flavonoid (TF) contents, the trolox equivalent antioxidant capacity (TEAC) and ferric reducing ability of plasma (FRAP) values. The daily portion of eight out of 14 products realized 45-135% of recommended daily allowance (RDA) for Fe. One of ready-to-drink beverages was also a good source of Mg (about 40% of RDA), and another one of Mn (about 70% of RDA). The concentrations of TP and TF in ready-to-drink beverages varied from 6.5 to 187 µg/mL, whereas in concentrates, from 5886 to 19,844 µg/mL. Dietary supplements or food products with fulvic acids may be a good source of antioxidant polyphenolic compounds and some minerals.
RESUMO
The aim of this study was to test the usability of fluorescence spectroscopy to evaluate the stability of cold-pressed rapeseed oil during storage. Freshly-pressed rapeseed oil was stored in colorless and green glass bottles exposed to light, and in darkness for a period of 6 months. The quality deterioration of oils was evaluated on the basis of several chemical parameters (peroxide value, acid value, K232 and K270, polar compounds, tocopherols, carotenoids, pheophytins, oxygen concentration) and fluorescence. Parallel factor analysis (PARAFAC) of oil excitation-emission matrices revealed the presence of four fluorophores that showed different evolution throughout the storage period. The fluorescence study provided direct information about tocopherol and pheophytin degradation and revealed formation of a new fluorescent product. Principal component analysis (PCA) performed on analytical and fluorescence data showed that oxidation was more advanced in samples exposed to light due to the photo-induced processes; only a very minor effect of the bottle color was observed. Multiple linear regression (MLR) and partial least squares regression (PLSR) on the PARAFAC scores revealed a quantitative relationship between fluorescence and some of the chemical parameters.
RESUMO
The effect of pH on the radical scavenging capacity of green tea catechins was investigated using experimental as well as theoretical methods. It was shown that the radical scavenging capacity of the catechins, quantified by the TEAC value, increases with increasing pH of the medium. Comparison of the pKa values to theoretically calculated parameters for the neutral and deprotonated forms indicates that the pH-dependent increase in radical scavenging activity of the catechins is due to an increase of electron-donating ability upon deprotonation. The data also reveal that the radical scavenging activity of the catechins containing the pyrogallol (or catechol) and the galloyl moiety over the whole pH range is due to an additive effect of these two independent radical scavenging structural elements. Altogether, the results obtained provide better insight into the factors determining the radical scavenging activity of the catechins and reveal that the biological activity of green tea catechins will be influenced by the pH of the surrounding medium or tissues.
Assuntos
Catequina/farmacologia , Sequestradores de Radicais Livres/farmacologia , Chá/química , Concentração de Íons de HidrogênioRESUMO
Folic acid (FA) is used, in many countries, in nutritional supplements or for the fortification of cereals and their products. It is also used in vitamin pills. Recently, it was reported that folates may act as antioxidants; therefore, in the present study, the effect of pH of the surrounding medium on the radical-scavenging activity of FA and its reduced forms [dihydrofolic acid (DHF), tetrahydrofolic acid (THF), 5-methyltetrahydrofolic acid (5-MTHF), and 5-formyltetrahydrofolic acid (5-FTHF)] was investigated. It was found that radical-scavenging activities of folates, measured in the trolox equivalent antioxidant capacity (TEAC) assay, are strongly pH-dependent. FA is a better radical scavenger at acid and basic pH than at neutral pH. Reduced forms of FA are better radical scavengers at acidic pH values than at neutral and basic pH values, with exception of 5-FTHF for which, at a pH higher than 5.0, an increase of the radical-scavenging activity with an increasing pH of the medium is observed. The results of the present study indicate that possible health effects of folates associated with their radical-scavenging activity will vary depending upon the pH of body fluid or tissue considered.
Assuntos
Ácido Fólico/análogos & derivados , Ácido Fólico/farmacologia , Ácido Fólico/química , Sequestradores de Radicais Livres/química , Sequestradores de Radicais Livres/farmacologia , Concentração de Íons de Hidrogênio , Oxirredução , Relação Estrutura-Atividade , Tetra-Hidrofolatos/farmacologiaRESUMO
Gluten-free (GF) products are those with a natural absence or acceptable level (<20 mg/kg) of gluten. They should be a part of a diet for people with gluten-related disorders, like celiac disease. Recently the popularity of a gluten-free diet (GFD) has risen extremely, because a lot of healthy individuals exclude gluten from their menus. According to the literature data on nutritional deficiencies in the GFD, this trend seems to be risky. This paper describes the nutritional value of 14 flours from different GF raw materials from the aspect of B-group vitamin content (B1, B2, B3, B6). Vitamins were determined using high performance liquid chromatography after enzymatic and acid hydrolysis of the samples. The vitamin contents significantly differed in the analysed flours. The content (in 100 g of the product) of vitamin B1 ranged from 0.01 mg (amaranth flour) to 0.60 mg (teff flour), vitamin B2 from 0.03 mg (GF flour with oats) to 0.22 mg (buckwheat flour), vitamin B3 from below 0.01 mg (amaranth flour) to 6.02 mg (millet flour), and vitamin B6 from 0.03 mg (acorn flour) to 0.69 mg (amaranth flour). The content of vitamins in the analysed GF flours was also compared to gluten-containing flours. Obtained results indicate that flours from teff, millet, chestnut, buckwheat, and amaranth are better sources of certain B-group vitamins than flours from corn, rice, and some flours with gluten.
Assuntos
Farinha/análise , Glutens/análise , Niacinamida/análise , Riboflavina/análise , Tiamina/análise , Vitamina B 6/análise , Amaranthus/química , Avena/química , Dieta Livre de Glúten , Fagopyrum/química , Milhetes/química , Valor Nutritivo , Recomendações Nutricionais , Reprodutibilidade dos Testes , Complexo Vitamínico B/análiseRESUMO
BACKGROUND: Plants are an important source of phenolic compounds. The antioxidant capacities of green tea, thyme and rosemary extracts that contain these compounds have been reported earlier. However, there is a lack of accessible information about their activity against lipid oxidation in emulsions and inhibit the interaction of lipid oxidation products with amino acids. Therefore, the influence of green tea, thyme and rosemary extracts and BHT (butylated hydroxytoluene) on quantitative changes in lysine and methionine in linoleic acid emulsions at a pH of isoelectric point and a pH lower than the isoelectric point of amino acids was investigated. METHODS: Total phenolic contents in plant extracts were determined spectrophotometrically by using Folin-Ciocalteu's reagent, and individual phenols by using HPLC. The level of oxidation of emulsion was determined using the measurement of peroxides and TBARS (thiobarbituric acid reactive substances). Methionine and lysine in the system were reacted with sodium nitroprusside and trinitrobenzenesulphonic acid respectively, and the absorbance of the complexes was measured. RESULTS: Extract of green tea had the highest total polyphenol content. The system containing antioxidants and amino acid protected linoleic acid more efficiently than by the addition of antioxidants only. Lysine and methionine losses in samples without the addition of antioxidants were lower in their isoelectric points than below these points. Antioxidants decrease the loss of amino acids. The protective properties of antioxidants towards methionine were higher in a pH of isoelectric point whereas towards lysine in pH below this point. CONCLUSIONS: Green tea, thyme and rosemary extracts exhibit antioxidant activity in linoleic acid emulsions. Moreover, they can be utilized to inhibit quantitative changes in amino acids in lipid emulsions. However, the antioxidant efficiency of these extracts seems to depend on pH conditions. Further investigations should be carried out to clarify this issue.
Assuntos
Antioxidantes/química , Lisina/análise , Metionina/análise , Hidroxitolueno Butilado/química , Emulsões , Concentração de Íons de Hidrogênio , Ácido Linoleico/análise , Extratos Vegetais/química , Polifenóis/química , Rosmarinus/química , Chá/química , Substâncias Reativas com Ácido Tiobarbitúrico/análise , Thymus (Planta)/químicaRESUMO
The study demonstrates the application of front face and right angle synchronous fluorescence spectroscopy for the characterization of edible oils. The method enables monitoring of tocopherols, pheophytins, and other fluorescent components in edible oils. Principal component analysis of synchronous fluorescence spectra revealed sample clustering according to the type of oil. Partial least-squares regression was utilized to develop calibration models between fluorescence spectra and total tocopherol content determined by HPLC. The regression models showed a good ability to predict tocopherol content. The best fitting results were obtained for 1% v/v diluted oils and for bulk samples using the entire spectrum, yielding the regression coefficient, r, of 0.991, and root mean square error of cross-validation of approximately 8%. The results presented confirm the capabilities of the fluorescence techniques as a tool for the analysis of edible oils.
Assuntos
Óleos de Plantas/química , Espectrometria de Fluorescência/métodos , Tocoferóis/análise , Cromatografia Líquida de Alta PressãoRESUMO
Products containing natural additives, including antioxidants, are usually perceived by consumers as safer than those with synthetic ones. Natural antioxidants, besides having a preservative activity, may exert beneficial health effects. Interactions between antioxidants may significantly change their antioxidant activity, thus in designing functional foods or food/cosmetic ingredients knowledge about the type of interactions could be useful. In the present study, the interactions between ascorbic acid (AA; vitamin C) and different black and green tea extracts and the influence on their antioxidant activities were investigated. The antioxidant activities of tea extracts and their mixtures with AA prepared in several different weight ratios were measured using the trolox equivalent antioxidant capacity (TEAC), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and ferric-reducing antioxidant power (FRAP) methods. The type of interaction was determined by interaction indexes and isobolograms. It was found that the weight ratio of extracts to AA significantly influenced the antioxidant activity of a mixture and the type of interaction between these components. The weight ratio of tea extract to AA can cause the change of interaction, e.g. from antagonism to additivism or from additivism to synergism. The observed differences in the type of interactions were probably also a result of different extracts' polyphenol composition and content. The type of interaction may also be affected by the medium in which extracts and AA interact, especially its pH and the solvent used. To obtain the best antioxidant effect, all these factors should be taken into account during the design of a tea extract-AA mixture.
Assuntos
Antioxidantes/química , Ácido Ascórbico/química , Camellia sinensis/química , Extratos Vegetais/química , Folhas de Planta/química , Antioxidantes/farmacologia , Ácido Ascórbico/farmacologia , Compostos de Bifenilo/química , Interações Medicamentosas , Sinergismo Farmacológico , Compostos Férricos/química , Alimento Funcional/análise , Concentração de Íons de Hidrogênio , Picratos/química , Extratos Vegetais/farmacologia , Polifenóis/análise , Polifenóis/química , Polifenóis/farmacologia , Solventes , Chá/químicaRESUMO
Ultra performance liquid chromatography (UPLC) and high-performance liquid chromatography (HPLC) methods for determination of ascorbic acid (AA) and total AA (TAA) contents (as the sum of AA and dehydroascorbic acid (DHAA) after its reduction to AA) in fruit beverages and in pharmaceutical preparations were compared. Both methods are rapid: total time of analysis was 15 and 6 min for HPLC and UPLC methods, respectively. The methods were validated in terms of linearity, instrument precision, limits of detection (LOD) and quantification (LOQ), accuracy and recovery. Intra- and inter-day instrument precisions for fruit juices, expressed as RSD, were 2.2% and 2.4% for HPLC, respectively, and 1.7% and 1.9% for UPLC, respectively. For vitamin C tablets, inter- and intra-day precisions were 0.4% and 0.5%, respectively (HPLC), and 0.5% and 0.3%, respectively (UPLC). Both methods were sensitive: LOD was 0.049 µg/mL for HPLC and 0.024 µg/mL for UPLC while LOQs were 0.149 and 0.073 µg/mL for HPLC and UPLC, respectively. These methods could be useful in the routine qualitative and quantitative analysis of AA or TAA in pharmaceutical preparations or fruit beverages. However, UPLC method is more sensitive, faster and consumes less eluent.
Assuntos
Ácido Ascórbico/química , Cromatografia Líquida de Alta Pressão/métodos , Cromatografia Líquida/métodos , Análise de Alimentos/métodos , Vitaminas/química , Ácido Ascórbico/análise , Frutas/química , Vitaminas/análiseRESUMO
Optimal in vitro conditions regarding quercetin solubility and stability were defined. Using these conditions, the effect of quercetin on proliferation of the colon carcinoma cell lines HCT-116 and HT29 and the mammary adenocarcinoma cell line MCF-7 was investigated. For the colon carcinoma cell lines, at relatively high concentrations, a significant decrease in cell proliferation was observed, providing a basis for claims on the anti-carcinogenic activity of quercetin. However, at lower concentrations, a subtle but significant stimulation of cell proliferation was observed for all cell lines tested. These results point at a dualistic influence of quercetin on cell proliferation that may affect present views on its supposed beneficial anti-proliferative effect.
Assuntos
Adenocarcinoma/patologia , Neoplasias da Mama/patologia , Carcinoma/patologia , Neoplasias do Colo/patologia , Quercetina/farmacologia , Divisão Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Células HT29 , Humanos , Quercetina/química , Solubilidade , Células Tumorais CultivadasRESUMO
The biological effect of flavonoids can be modulated in vivo due to metabolism. The O-methylation of the catechol group in the molecule by catechol O-methyl transferase is one of the important metabolic pathways of flavonoids. In the present study, the consequences of catechol O-methylation for the pH-dependent radical scavenging properties of quercetin and luteolin were characterized both experimentally and theoretically. Comparison of the pKa values to the pH-dependent TEAC profiles reveals that O-methylation not only affects the TEAC as such but also modulates the effect of changing pH on this radical scavenging activity due to an effect on the pKa for deprotonation. The pH-dependent TEAC curves and computer calculated electronic parameters: bond dissociation energy (BDE) and ionisation potential (IP) even indicate that O-methylation of the luteolin catechol group affects the radical scavenging potential only because it shifts the pKa for deprotonation. O-Methylation of the quercetin catechol moiety affects radical scavenging capacity by both an effect on the pKa, and also by an effect on the electron and hydrogen atom donating properties of the neutral (N) and the anionic (A) form of the molecule. Moreover, O-methylation of a catechol OH-group in quercetin and luteolin has a similar effect on their TEAC profiles and on calculated parameters as replacement of the OH-group by a hydrogen atom. Altogether, the results presented provide new mechanistic insight in the effect of catechol O-methylation on the radical scavenging characteristics of quercetin and luteolin.
Assuntos
Catecóis/química , Sequestradores de Radicais Livres/química , Luteolina/química , Quercetina/química , Antioxidantes/química , Antioxidantes/metabolismo , Apigenina/química , Apigenina/metabolismo , Catecóis/metabolismo , Flavonoides/química , Flavonoides/metabolismo , Sequestradores de Radicais Livres/metabolismo , Hidrogênio/química , Hidrogênio/metabolismo , Concentração de Íons de Hidrogênio , Quempferóis/química , Quempferóis/metabolismo , Luteolina/metabolismo , Metilação , Estrutura Molecular , Quercetina/metabolismoRESUMO
A simple and rapid reversed-phase high-performance liquid chromatography method for determination of alpha-, (beta + gamma), and delta-tocopherols in edible plant oils has been developed. Oils are diluted in 2-propanol and injected directly onto Symmetry C18 column. Methanol and acetonitrile (1:1) are used as a mobile phase. Tocopherols are detected using fluorescence detector set at excitation and emission wavelength 295 nm and 325 nm, respectively. The method is precise (R.S.D. not higher than 2.24%) and sensitive-detection limits (DL) are 8 ng/ml for gamma- and delta-tocopherols and 28 ng/ml for alpha-tocopherol; quantification limits (QL) were calculated as three times higher than DL.
Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Óleos de Plantas/química , Tocoferóis/análise , 2-Propanol/química , Acetonitrilas/química , Calibragem , Metanol/química , Reprodutibilidade dos TestesRESUMO
The effects of quercetin on viability and proliferation of Chinese Hamster Ovary (CHO) cells and CHO cells overexpressing human quinone reductase (CHO+NQO1) were studied to investigate the involvement of the pro-oxidant quinone chemistry of quercetin. The toxicity of menadione was significantly reduced in CHO+NQO1 cells compared to wild-type CHO cells, validating the NQO1-overexpression in the CHO+NQO1 transfectant. Quercetin inhibited the proliferation of wild-type CHO and CHO+NQO1 cells to a similar extent without affecting cell viability, indicating that NQO1 enrichment of CHO cells did not provide increased protection. On the other hand, inhibition of NQO1 in both types of cells by dicoumarol significantly potentiated the inhibitory effect of quercetin on cell proliferation, revealing the role of NQO1 in cellular protection against quercetin. Altogether, these results can be explained by the hypothesis that both wild-type CHO and CHO+NQO1 cells contain sufficient NQO1 activity for optimal protection against the pro-oxidant effect of quercetin on cell proliferation. The results also point at a cellular NQO1 threshold for optimal protection against quercetin. This NQO1 threshold seems to be in the range of NQO1 activities already present in various tissues.