RESUMO
DNA from purified mitochondria of Drosophila melanogaster can be isolated as supercoiled molecules which when nicked have a contour length of 5.9 micron. Partial denaturation mapping shows regional heterogeneity of base composition with one early denaturing region, with a calculated GC content close to zero, extending over 20% of the genome. DNA isolated from unfertilized eggs shows nuclear and mitochondrial DNA in equal proportions; we found no evidence of other cytoplasmic species.
Assuntos
DNA Mitocondrial/análise , Drosophila melanogaster/análise , Animais , Citosina/análise , DNA/análise , Feminino , Guanina/análise , Conformação de Ácido Nucleico , Desnaturação de Ácido Nucleico , Renaturação de Ácido Nucleico , Óvulo/análiseRESUMO
A series of petite mutants of Saccharomyces cerevisiae, generated after treatment for various times with ethidium bromide, was isolated, and the mitochondrial deoxyribonucleic acid size for each member was estimated. It was found that, as the treatment time with ethidium bromide was increased, the mitochondrial deoxyribonucleic acid isolated from the petite series was increasingly reduced in size.