RESUMO
A commercially available kit consisting of twenty 10-mer random primers was evaluated to allow selection of a suitable primer that would permit identification and sub-typing of Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium by randomly amplified polymorphic DNA (RAPD). A primer OPE-20 (5'-AAC-GGT-GAC-C-3') was identified to be the most suitable primer when tested with four ATCC reference strains of M. paratuberculosis and eight well characterized field strains each of M. paratuberculosis and M. avium. Primer OPE-20 was further tested for its ability to identify and subtype 200 field isolates of M. paratuberculosis. The fingerprint patterns of M. paratuberculosis (n=212) consisted of five unique common fragments (620, 450, 310, 230, 180bp) and nine variable fragments resulting in six distinct genotypes. The DNA fingerprints of M. avium (n=8) consisted of a single common fragment of 620bp, and 15 variable fragments resulting in six different genotypes. The cattle, human and goat isolates of M. paratuberculosis were genetically similar, but a sheep isolate had a different RAPD profile as compared to RAPD profiles from other species. RAPD was observed to be a rapid, reproducible and reliable technique for identification and sub-typing of M. paratuberculosis.
Assuntos
Mycobacterium avium subsp. paratuberculosis/genética , Paratuberculose/microbiologia , Técnica de Amplificação ao Acaso de DNA Polimórfico/veterinária , Animais , Bovinos , Doenças dos Bovinos/diagnóstico , Doenças dos Bovinos/microbiologia , Primers do DNA/química , DNA Bacteriano/química , DNA Bacteriano/isolamento & purificação , Eletroforese em Gel de Ágar/veterinária , Doenças das Cabras/diagnóstico , Doenças das Cabras/microbiologia , Cabras , Humanos , Processamento de Imagem Assistida por Computador , Mycobacterium avium subsp. paratuberculosis/química , Mycobacterium avium subsp. paratuberculosis/classificação , Técnica de Amplificação ao Acaso de DNA Polimórfico/métodos , Ovinos , Doenças dos Ovinos/diagnóstico , Doenças dos Ovinos/microbiologiaRESUMO
The histerid beetle Carcinops pumilio (Erichson) occurs naturally in poultry house manure and is an important predator of house fly eggs and larvae. Because efforts to commercially produce C. pumilio have been unsuccessful, one fly control strategy under consideration is the direct transport of adult C. pumilio between poultry houses to facilitate their establishment. However, we demonstrate that C. pumilio is a competent reservoir of Salmonella enteritidis (Gaertner). Adult C. pumilio exposed to S. enteritidis-inoculated house fly eggs harbored the bacterium externally and internally for up to 4 and 13 d, respectively, and feces were culture-positive for S. enteritidis for at least 14 d. This suggests that C. pumilio can be a reservoir of S. enteritidis; therefore its movement between poultry facilities should be carefully considered.
Assuntos
Besouros/microbiologia , Reservatórios de Doenças , Salmonella enteritidis/isolamento & purificação , Animais , Besouros/fisiologia , Fezes/microbiologia , Fezes/parasitologia , Moscas Domésticas , Abrigo para Animais , Larva , Óvulo , Pennsylvania , Aves Domésticas , Comportamento PredatórioRESUMO
Neospora caninum was found in fetal tissues of 34 of 688 cases of bovine abortion submitted to the Pennsylvania Animal Diagnostic Laboratory System during the period from May 1994 to November 1996. The aborted fetuses ranged in gestational age from 3 to 8 months. Microscopic lesions consisted primarily of encephalitis and myocarditis. A labeled (strept) avidin-biotin staining procedure using anti-N. caninum polyclonal rabbit serum revealed N. caninum organisms within the fetal brain (27 of 27), heart (10 of 13), placenta (5 of 6), kidney (2 of 2), liver (1 of 4) and skeletal muscle (1 of 1).