Effect of Lactobacillus rhamnosus GG on rBet v1 and rMal d1 specific IgA in the saliva of patients with birch pollen allergy.

BACKGROUND: Lactobacillus rhamnosus GG (LGG) has demonstrated promising results in the treatment and prevention of atopic eczema. OBJECTIVE: To study the effects of LGG on the oral immune response in adolescents and adults with birch pollen allergy combined with oral allergy syndrome. METHODS: Patients received either LGG (n = 19) or a placebo (n = 19) for 5.5 months (from February 8 to August 6, 1999), starting 2.5 months before the birch pollen season. An oral apple challenge test was performed before, during, and after the pollen season. Saliva samples were collected before and after the challenges, and serum samples were collected before the challenges. Total IgA, IgG, and IgM and rBet v1 and rMal dl specific IgA, IgG, IgG1, and IgG4 levels were measured from saliva with an enzyme-linked immunosorbent assay (ELISA). Serum rBet v1 specific IgE ELISA and birch radioallergosorbent testing were performed. RESULTS: After 5.5 months, rBet v1 and rMal dl specific IgA levels had increased from baseline in the LGG compared with the placebo group (delta rBet v1 IgA, 0.319 vs. -0.136 relative units; P = .02; delta rMal d1 IgA, 0.097 vs -0.117, P = .02). rBet v1 specific IgE serum levels did not differ between the groups. In the LGG group, rBet v1 specific IgE levels correlated positively with stimulated total IgA (P = .04) and IgG (P = .003) in saliva. In the placebo group, rBet vl specific IgE levels correlated negatively with stimulated rBet v1 and rMal d1 IgA levels (P = .009 for both) and IgG (P = .02 and P = .03, respectively). CONCLUSION: LGG showed immunostimulating effects on oral mucosa seen as increased allergen specific IgA levels in saliva.

Dental and oral radiographic findings in first-year university students in 1982 and 2002 in Helsinki, Finland.

OBJECTIVE: This study is part of a larger research program, at the Finnish Student Health Service in Helsinki, in which changes in the dental and oral health of first-year university students (born in Helsinki) in the years 1982 and 2002 were compared. MATERIAL AND METHODS: Panoramic radiographs were taken of 176 students in 1982, mean age 19.8 years (SD+/-0.7), and of 231 in 2002, mean age 20.2 (SD+/-0.7). DMFT index, caries and periapical changes, alveolar bone resorption, wisdom teeth, supernumerary, missing, and persisting deciduous teeth were evaluated from the radiographs. The chi-squared test and non-parametric tests were used to analyze the statistical significance of differences between the study groups and between genders. RESULTS: The DMFT index diminished from 11.0 (SD +/- 4.2) to 2.9 (SD +/- 3.3) (p < 0.0001). In 2002, 27.9% of the students had a DMFT index of zero (in 1982, 0%, p < 0.000). The mean number of endodontically treated teeth diminished from 0.1 to 0.03 (p < 0.05). The number of impacted wisdom teeth increased in males from mean 0.41 to 0.69 (p < 0.05). CONCLUSIONS: An improvement in dental health was obvious in the year 2002. The favorable change in DMFT index is a trend that has been going on for several decades in Finland, probably as a result of the use of fluorides and better education in dental hygiene. The change might also be connected with improved general health.

Oral piercings among first-year university students.

OBJECTIVE: The aim of the study was to examine oral piercings among first-year university students. STUDY DESIGN: First-year university students in 2002 were invited to a dental examination (n = 234; 49 men and 185 women). Students with piercings formed the study group and the rest served as controls. The methods included decayed, missing, and filled teeth (DMF) index, stimulated salivary flow rates, panoramic tomograms, and questionnaires including the Depression Inventory of Beck. Fisher's 2-sided exact test was used for statistical analysis. RESULTS: The prevalence of oral piercings was 3.4%. In the DMF indices, no statistically significant differences existed between the groups. Increased salivary flow rates were noted among students with piercings (63% vs 26%, P < .05). Use of tobacco and illicit drugs, and also depression, were more prevalent in the study group than in the controls. CONCLUSION: Because of the possibility of oral implications, follow-up of oral piercings is essential.