RESUMO
Live yeast cell derivative is an alcoholic extract from yeast (Saccharomyces cerevisiae) that has previously been shown by three groups of workers to stimulate wound healing. Live yeast cell derivative is a complex mixture, and it was not known which of its many components was responsible for the biological activity. This study describes the separation and analysis of the major components, one of which is a peptide fraction that stimulates wound healing. The fraction consists of a mixture of peptides from 6000 to 17,000 d. It causes angiogenesis in a chick embryo yolk sac membrane assay and in a rabbit cornea assay, and it dramatically stimulates wound healing in the "Schilling/Hunt" wire mesh cylinder model at concentrations 25-fold lower than those required for the intact live yeast cell derivative.
Assuntos
Peptídeos/isolamento & purificação , Saccharomyces cerevisiae/química , Cicatrização/efeitos dos fármacos , Animais , Produtos Biológicos , Embrião de Galinha , Neovascularização Patológica , Fragmentos de Peptídeos , Peptídeos/farmacologia , Coelhos , Ratos , Estimulação QuímicaAssuntos
Cartilagem/metabolismo , Glicosaminoglicanos/metabolismo , Animais , Sítios de Ligação , Radioisótopos de Carbono , Cartilagem/análise , Centrifugação com Gradiente de Concentração , Cromatografia em Gel , Orelha , Glucose , Glicosaminoglicanos/análise , Guanidinas , Substâncias Macromoleculares , Ligação Proteica , Coelhos , Cloreto de Sódio , Espectrofotometria Ultravioleta , Ácidos Urônicos/análiseRESUMO
A method is described whereby the ratio of the major interstitial collagens (Types I and III) can be measured in biopsy specimens of human tissue weighing as little as 25 mg. Marker peptides are solubilized from the tissue by digestion with cyanogen bromide. These peptides which are not known to be involved in collagen crosslinking are isolated and quantified by a combination of carboxymethyl-cellulose chromatography and polyacrylamide gel electrophoresis. The peptides used are alpha 1(I)-CB7 and alpha 1(III)-CB5. The use of the method is illustrated by analyzing the collagen type ratio in small specimens of tendon, aorta, and vena cava.
Assuntos
Arteriosclerose/metabolismo , Vasos Sanguíneos/análise , Colágeno/isolamento & purificação , Tendões/análise , Adulto , Aorta/análise , Brometo de Cianogênio , Eletroforese em Gel de Poliacrilamida , Humanos , Masculino , Microquímica , Veias Cavas/análiseRESUMO
The sulfated material which locates in the basal membrane of ciliary body epithelium when immature rat eyes are incubated with Na235SO4 was studied. On the basis of its chromatographic behavior compared with standard preparations, we conclude that the material consists of sulfatides.