Molecular pathotyping and phylogenesis of the first Newcastle disease virus strain isolated from backyard chickens in Qatar.

The first Newcastle disease virus (NDV) field strain (NDV-QC08), previously isolated and identified in Qatar, was further subjected to molecular characterization and phylogenic analysis at passage levels two. Initial amplification and detection of the complementary DNA (cDNA) fragment of the NDV RNA amnioallantoic fluid (AAF) extract demonstrated the positive virus identity using real-time reverse transcriptase chain reaction (RRT-PCR). Partial sequence of the totally amplified 216 bp of the functional cleavage region encoding the F gene retrieved (112)R-R-Q-K-R-F(117) amino acid motif indicating the velogenic nature of the strain, and confirming the previously investigated in-vivo pathotyping. Phylogenic analysis and genotypic clustering studies classified the strain under NDV subgenotype VIId.

High biosorption of cationic dye onto a novel material based on paper mill sludge.

The valorization of paper mill sludge (PMS) is the main goal of this study. The emissions of PMS continue to increase at global scale, especially from packaging paper and board sectors. The raw sludge was used to prepare an adsorbent to remove toxic pollutants from wastewater, the methylene blue (MB), an organic dye. Firstly, the physico-chemical characterization of PMS was done determining the crystalline phases of PMS fibers, the content of main elements, and the pH zero point charge, which was determined at around pH 7. The adsorption of MB on PMS powder was studied at 18 °C with an agitation of 200 rpm, being the best operating conditions 30 min of contact time, 250 mg L-1 of initial MB concentration and 0.05 g in 25 mL of adsorbent dose. Experimental data of MB adsorption was fitted to Langmuir and Freundlich isotherm equations. The Langmuir model was more accurate for the equilibrium data of MB adsorption at pH 5.1. The PFOM and PSOM were adjusted to experimental adsorption kinetics data, being PSOM, which describes better the MB adsorption by PMS powder. This was confirmed by calculating the maximum adsorption capacity with PSOM, which was 42.7 mg g-1, being nearly similar of the experimental value of 43.5 mg g-1. The analysis of adsorption thermodynamics showed that the MB was adsorbed exothermically with a ΔH0 = - 20.78 kJ mol-1, and spontaneously with ΔG0 from - 0.99 to - 6.38 kJ mol-1 in the range of temperature from 291 to 363 K, respectively. These results confirm that the sludge from paper industry can be used as biosorbent with remarkable adsorption capacity and low cost for the treatment of wastewater. PMS can be applied in the future for the depollution of the effluents from the textile industry, which are highly charged with dyes.

Experimental Investigation of a Self-adaptive Thickening Polymer Performance under High-Temperature-High-Salinity Reservoir Conditions.

A polymer flooding workflow was developed to diminish polymer degradation and minimize formation damage under high-temperature-high-salinity reservoir conditions by using a shear-thickening polymer (SAP) prepared in engineered waters. First, rock characterization, fluid-fluid analysis, and formation damage tests were conducted to shortlist the potential formulations of polymer solutions based on higher viscosity and less formation damage. Second, polymer core flooding experiments were conducted under reservoir conditions to investigate the performance of candidate polymer solutions on oil displacement efficiency (DE). For the first time, the compatibility between SAP and engineered water was systematically tested. The factors affecting bulk rheology, polymer retention, and oil DE, including polymer concentration, polymer type, salinity, and hardness, were experimentally investigated and compared with regular partially hydrolyzed polyacrylamide (HPAM). Results showed that compared with HPAM, the SAP solution led to lower formation damage and overall higher oil DE, especially in the first 0.4 pore volume of polymer injection. When using SAP prepared in twice-diluted and hardness-stripped seawater under low-salinity formation brine conditions, the DE was the highest (69.04%). The formation damage was reduced when the salinity and hardness of the base fluid were lower, whereas stripping the hardness had a more pronounced effect on reducing formation damage. The improved oil recovery potential due to the shear-thickening feature of SAP solutions and their better compatibility with engineered water compared to regular HPAM has been proven in this study. It was also found that the lower salinity and hardness of the engineered water further stimulated the enhanced oil recovery potential of SAP solutions. The contribution of this work relies on revealing how SAP prepared in different engineered waters affects incremental oil DE under harsh reservoir conditions based on experimental evidence and mechanism analysis. The novelty of this work lays the foundation for investigating the potential application of SAP on a pilot scale.

First serological evidence of MERS-CoV in dromedary camels from Algeria.

Middle East Respiratory Syndrome (MERS) is a zoonotic disease. Dromedary camel is responsible of its transmission to humans. Accordingly, several human cases have been reported worldwide with a high mortality rate. In Algeria, no data reported on MERS prevalence in camels. This is a first seroprevalence study MERS-CoV in Algerian dromedaries. A total of 87 camel blood samples from EL -MENIAA and Ghardaia, were analyzed by anti-MERS-CoV IgG ELISA camel. The seroprevalence was 64 % and it significantly increases with age. Larger serological and molecular screening is needed to precisely determine the rate of MERS active circulation among Algerian dromedary population.

Investigation of Hybrid Nanoparticle-Acid Fluids (HNAFs): Influence of Wettability and Interfacial Tension Mechanisms in Harsh Carbonate Reservoirs for Improved Oil Recovery.

Over the past few years, there has been significant interest in the potential of hybrid nanoparticle-acid fluid (HNAFs) for improved oil recovery. This comprehensive study investigates the effects of nanoparticles and acid on interfacial tension (IFT) to establish a relationship between brine properties and the oil/brine IFT. This investigation is one of the first regional studies conducted utilizing candidate field data from the Middle East. Based on the literature review and screening studies conducted, a seawater (SW)-based HNAF was formulated with nanoparticles (SiO2, Al2O3, and ZnO) and HCl to measure their effect on IFT. A total of 48 formulations of HNAFs, nanofluids with and without acid, were analyzed with crude oil from a candidate field. IFT measurements were subsequently conducted using the pendant drop method under ambient conditions and in a high-pressure, high-temperature reservoir environment. Results showcased that IFT reduction was observed by increasing the acid concentration with SiO2 and Al2O3, although a reverse trend was observed with ZnO. Moreover, it was observed that IFT varied with increasing concentrations of nanoparticles, and at certain acid concentrations, IFT reduced significantly with higher nanoparticle concentrations. From the Amott studies, a clear signature was achieved, with ZnO exhibiting a total of 31.4% oil recovery, followed by SiO2 (27.3%) and Al2O3 (23.7%). The results of this study may assist in defining a screening criterion for future displacement (oil recovery) studies involving the presented nanoparticles. The results also reveal further the mechanisms involved in IFT reduction by hybrid nano-acid fluids and their potential for significant applications in the Middle East.

SARS-CoV-2 at the human-animal interphase: A review.

Since its emergence in China in December 2019, COVID-19 remains the recent leading disease of concern drawing the public health attention globally. The disease is known of viral origin and zoonotic nature originating from animals. However, to date neither the source of the spillover nor the intermediate hosts are identified. Moreover, the public health situation is intermittently aggravated by identification of new animals susceptible to the SARS-CoV-2 infection, potentially replicating the virus and maintaining intra and interspecies spread of the disease. Although the role of a given animal and/or its produce is important to map the disease pattern, continuous efforts should be undertaken to further understand the epidemiology of SARS-CoV-2, a vital step to establish effective disease prevention and control strategy. This manuscript attempted to review updates regarding SARS-CoV-2 infection at the human-animal interface with consideration to postulations on the genetic relatedness and origin of the different SARS-CoV-2 variants isolated from different animal species. Also, the review addresses the possible role of different animal species and their produce in transmission of the disease. Also, the manuscript discussed the contamination potentiality of the virus and its environmental stability. Finally, we reviewed the currently instituted measures to prevent and manage the spread of SARS-CoV-2 infection. The manuscript suggested the One Health based control measures that could prove of value for the near future.

Morphometric Study of Mus musculus, Rattus norvegicus, and Rattus rattus in Qatar.

The current study was undertaken to estimate the morphometric pattern of three commensal rodents, i.e., Mus musculus, Rattus norvegicus, and Rattus rattus in Qatar. One hundred forty-eight rodents were captured from different facilities throughout Qatar. The captured rodents were used to identify the external body and cranio-mandibular morphometry. The study found that R. norvregicus was the most prevalent (n = 120, 81%, 95% CI: 73.83-87.05). Most of the rodents were collected from Al Rayan municipality (n = 92, 62%), were adults (n = 138, 93.2%, 95% CI: 87.92-96.71), and were from livestock farms (n = 79, 49%, 95% CI: 41.02-57.65). The rodents' average body weights were 18.8 ± 2.2 gm, 264.3 ± 87.5 gm, and 130 ± 71.3 gm for M. musculus, R. norvegicus, and R. rattus, respectively. The research found that the studied rodents are smaller than those of other countries such as Turkey, Tunisia, and Iran. The study of morphometry is a useful tool for the traditional identification of small mammal species, including rodents. The average morphometric measurements of the external body and skull were normally distributed and can be used as a reference of R. norvegicus and R. rattus for Qatar. A further comprehensive study is required to investigate the rodent population index, eco-friendly control program, and public health importance in Qatar.

Qatar experience on One Health approach for middle-east respiratory syndrome coronavirus, 2012-2017: A viewpoint.

The emergence of the Middle East Respiratory Syndrome Corona Virus (MERS-CoV) in the Middle East in 2012 was associated with an overwhelming uncertainty about its epidemiological and clinical characteristics. Once dromedary camels (Camelus dromedarius) was found to be the natural reservoir of the virus, the public health systems across the Arabian Peninsula encountered an unprecedented pressure to control its transmission. This view point describes how the One Health approach was used in Qatar to manage the MERS-CoV outbreak during the period 2012-2017. One Health focuses on the association between the human, animals and environment sectors for total health and wellbeing of these three sectors. To manage the MERS outbreak in Qatar through a One Health approach, the Qatar National Outbreak Control Taskforce (OCT) was reactivated in November 2012. The animal health sector was invited to join the OCT. Later on, technical expertise was requested from the WHO, FAO, CDC, EMC, and PHE. Subsequently, a comprehensive One Health roadmap was delivered through leadership and coordination; surveillance and investigation; epidemiological studies and increase of local diagnostic capacity. The joint OCT, once trained had easy access to allocated resources and high risk areas to provide more evidence on the potential source of the virus and to investigate all reported cases within 24-48 h. Lack of sufficient technical guidance on veterinary surveillance and poor risk perception among the vulnerable population constituted major obstacles to maintain systematic One Health performance.

Copper(II) complexes of lipophilic aminoglycoside derivatives for the amino acid enantiomeric separation by ligand-exchange liquid chromatography.

In this paper, a new class of ligand-exchange chiral stationary phase (LE-CSP) based on the copper complexes of lipophilic aminoglycoside derivatives was reported. Different stationary phases were developed by coating reversed-phase liquid chromatography supports with three neamine derivatives carrying a lipophilic octadecyl chain at the 4', 5 and 6 positions, respectively. The enantioselective ability of these LE neamine-based CSPs was evaluated and the 4'-derivative coated column was found to be the most interesting one for the amino acid resolution. The effects of the variation of several chromatographic parameters on the enantioseparation were evaluated in order to identify the analysis optimal conditions.

Salt effects on the interaction of an amphiphilic model molecule with immobilized phosphatidylcholine monolayers.

The retention of hydrocortisone (used as an amphiphilic model solute) on an immobilized artificial membrane (IAM) column was investigated in relation to the mobile phase concentration of three sodium salts (representing different rankings in the Hofmeister series, i.e. perchlorate, chloride and sulfate) in order to provide insight into the nature of the solute interactions with phospholipid monolayers. The influence of the salt series on solute retention was found to follow the Hofmeister series, emphasizing the role of hydrophobic effect in the solute retention mechanism on phospholipid monolayers. Retention models based on the extended Wyman relations (preferential interaction theory) were developed to analyze more quantitatively the salt effects on the hydrocortisone retention factor. This analysis as well as additional thermodynamic study suggested that the hydrocortisone binding to IAM involved both an insertion into the hydrophobic inside governed by hydrophobic effects and contacts with the interfacial region implying interactions such as van der Waals interactions/hydrogen bonds between the solute hydroxyl groups and the polar headgroups of phospholipidmonolayers.

Distraction osteogenesis as followed by CT scan in Pierre Robin sequence.

THE AIM: The aim of this work was to assess the multislice CT scan for analysis of the craniofacial anatomic changes in Pierre Robin sequence both predistraction and postdistraction, and to assess the use of unidirectional internal distractors in this patient group. PATIENTS & METHODS: The study involved 11 patients. Their age at the time of distraction ranged from 2 to 7 months. Six were females and 5 were males. All had retromicrognathia, glossoptosis and obstructive sleep apnoea. All were diagnosed clinically and by CT scan. All were managed by distraction osteogenesis and were followed postoperatively by multislice CT. RESULTS: The distance between the base of the tongue and the posterior pharyngeal wall increased by a mean of 141%, and the total mandibular length increased by a mean of 26%. The increase in the distance between the hyoid bone and the posterior pharyngeal wall increased by a mean of 42% .The distance between the hyoid bone and the genoid process increased by a mean of 9%. CONCLUSION: Multislice CT scan was found to be a practical imaging technique to evaluate the morphologic changes in the airway and the mandible after distraction osteogenesis. It rules out the need for other traditional methods. Owing to the plasticity and malleability of the infant mandible that allow for sufficient bone remodelling, unidirectional internal distractors achieved a satisfactory maxillomandibular relationship which was tolerated by the infants and accepted by the parents.

Reversal of the enantiomeric elution order of some aromatic amino acids using reversed-phase chromatographic supports coated with the teicoplanin chiral selector.

In this paper, two chiral stationary phases were prepared by coating the surface of both C8 and C18 high-performance liquid chromatography (HPLC) supports with the teicoplanin chiral selector. The hydrophobic C11 acyl side chain, attached to the D-glucosamine group of teicoplanin, served as anchor moiety for the immobilization of the chiral selector on the apolar support material. The retention and enantioselectivity of these coated stationary phases were studied using some aromatic amino acids as probe solutes and an aqueous solution as mobile phase. It was found that the enantiomer elution order on the modified C8 and C18 stationary phases was reversed (L>D) relatively to that classically observed with a teicoplanin covalently immobilized on a silica support (D>L). Such a dynamic coating on the reversed-phase supports was found to be of interest since the apparent enantioselectivity was not significantly changed by the use during an extended period of time or following a long-term storage of the columns.

Thermodynamic origin of the chiral recognition of tryptophan on teicoplanin and teicoplanin aglycone stationary phases.

The D-, L-tryptophan binding and the chiral recognition properties of the teicoplanin and teicoplanin aglycone (TAG) chiral stationary phase (CSPs) were compared at various column temperatures. The solute adsorption isotherms (bi-Langmuir model) were determined for both the two CSPs using the perturbation method. It was demonstrated that the sugar units were involved in the reduction of the apparent enantioselectivity through two phenomena: (i) the inhibition of some enantioselective contacts with low-affinity binding regions of the aglycone and (ii) a decrease in the stereoselective properties of the aglycone high-affinity binding pocket. The phenomenon (ii) was governed by both a decrease in the ratio of the enantiomer adsorption constant and a strong reduction of the site accessibility for D- and L-tryptophan. In addition, a temperature effect study was performed to investigate the chiral recognition mechanism at the aglycone high-affinity pocket. An enthalpy-entropy compensation analysis derived from the Grunwald model as well as the comparison with the literature data demonstrated that the enantioselective binding mode was dependent on an interface dehydration process. The change in the enantioselective process observed between the TAG and teicoplanin CSP was characterized by a difference of ca. 2-3 ordered water molecules released from the species interface.