RESUMO
The microbiota is suggested to be a fundamental contributor to host reproduction and survival, but associations between microbiota and fitness are rare, especially for wild animals. Here, we tested the association between microbiota and two proxies of breeding performance in multiple body sites of the black-legged kittiwake, a seabird species. First we found that, in females, nonbreeders (i.e., birds that did not lay eggs) hosted different microbiota composition to that of breeders in neck and flank feathers, in the choanae, in the outer-bill and in the cloacae, but not in preen feathers and tracheae. These differences in microbiota might reflect variations in age or individual quality between breeders and nonbreeders. Second, we found that better female breeders (i.e., with higher body condition, earlier laying date, heavier eggs, larger clutch, and higher hatching success) had lower abundance of several Corynebacteriaceae in cloaca than poorer female breeders, suggesting that these bacteria might be pathogenic. Third, in females, better breeders had different microbiota composition and lower microbiota diversity in feathers, especially in preen feathers. They had also reduced dispersion in microbiota composition across body sites. These results might suggest that good breeding females are able to control their feather microbiota-potentially through preen secretions-more tightly than poor breeding females. We did not find strong evidence for an association between reproductive outcome and microbiota in males. Our results are consistent with the hypothesis that natural variation in the microbiota is associated with differences in host fitness in wild animals, but the causal relationships remain to be investigated.
Assuntos
Animais Selvagens , Microbiota , Animais , Masculino , Feminino , Aves , Microbiota/genética , Bactérias , Plumas/microbiologia , ReproduçãoRESUMO
Seasonal timing of breeding is usually considered to be triggered by endogenous responses linked to predictive cues (e.g., photoperiod) and supplementary cues that vary annually (e.g., food supply), but social cues are also important. Females may be more sensitive to supplementary cues because of their greater role in reproductive timing decisions, while males may only require predictive cues. We tested this hypothesis by food-supplementing female and male colonial seabirds (black-legged kittiwakes, Rissa tridactyla) during the pre-breeding season. We measured colony attendance via GPS devices, quantified pituitary and gonadal responses to gonadotropin releasing hormone (GnRH) challenge, and observed subsequent laying phenology. Food supplementation advanced laying phenology and increased colony attendance. While female pituitary responses to GnRH were consistent across the pre-breeding season, males showed a peak in pituitary sensitivity at approximately the same time that most females were initiating follicle development. The late peak in male pituitary response to GnRH questions a common assumption that males primarily rely on predictive cues (e.g., photoperiod) while females also rely on supplementary cues (e.g., food availability). Instead, male kittiwakes may integrate synchronising cues from their social environment to adjust their reproductive timing to coincide with female timing.
Assuntos
Charadriiformes , Hormônio Liberador de Gonadotropina , Animais , Feminino , Masculino , Hormônio Liberador de Gonadotropina/farmacologia , Hormônio Liberador de Gonadotropina/metabolismo , Reprodução/fisiologia , Hipófise/metabolismo , FotoperíodoRESUMO
The ability to efficiently measure the health and nutritional status of wild populations in situ is a valuable tool, as many methods of evaluating animal physiology do not occur in real-time, limiting the possibilities for direct intervention. This study investigates the use of blood plasma metabolite concentrations, measured via point-of-care devices or a simple plate reader assay, as indicators of nutritional state in free-living seabirds. We experimentally manipulated the energy expenditure of wild black-legged kittiwakes on Middleton Island, Alaska, and measured the plasma concentrations of glucose, cholesterol, B-hydroxybutyrate, and triglycerides throughout the breeding season, along with measures of body condition (size-corrected mass [SCM] and muscle depth). Supplemental feeding improved the nutritional state of kittiwakes by increasing feeding rate (higher glucose and triglycerides, lower cholesterol), and flight-handicapping caused a slight nutritional decline (lower glucose and triglycerides, higher cholesterol and B-hydroxybutyrate). Glucose and triglycerides were the best indicators of nutritional state when used alongside SCM, and improved upon commonly used metrics for measuring individual condition (i.e. SCM or mass alone). Metabolite concentrations varied across the breeding period, suggesting that the pre-laying stage, when feeding rates tend to be lower, was the most nutritionally challenging period for kittiwakes (low glucose, high cholesterol). Muscle depth also varied by treatment and breeding stage, but differed from other nutritional indices, suggesting that muscle depth is an indicator of exercise and activity level rather than nutrition. Here we demonstrate potential for the use of blood plasma metabolites measured via point-of-care devices as proxies for evaluating individual health, population health, and environmental food availability.
Assuntos
Colesterol , Estado Nutricional , Animais , Triglicerídeos , Hidroxibutiratos , AvesRESUMO
Breeding is costly for many animals, including birds that must deliver food to a central place (i.e. nest). Measuring energy expenditure throughout the breeding season can provide valuable insight into physiological limitations by highlighting periods of high demand, and ultimately allows improvement of conservation strategies. However, quantifying energy expenditure in wildlife can be challenging, as existing methods do not measure both active (e.g. foraging) and resting energy costs across short and long time scales. Here, we developed a novel method for comparing active and resting costs in 66 pre-breeding and breeding seabirds (black-legged kittiwakes, Rissa tridactyla) by combining accelerometry and triiodothyronine (T3) as proxies for active and resting costs, respectively. Active energy costs were higher during incubation (P=0.0004) and chick rearing (P<0.0001) than during pre-laying, because of an increase in the time spent in flight of 11% (P=0.0005) and 15% (P<0.0001), respectively. Levels of T3, reflecting resting costs, peaked marginally during incubation with a mean (±s.d.) concentration of 4.71±1.97â pgâ ml-1 in comparison to 2.66±1.30â pgâ ml-1 during pre-laying (P=0.05) and 3.16±2.85â pgâ ml-1 during chick rearing (P=0.11). Thus, although chick rearing is often assumed to be the costliest breeding stage by multiple studies, our results suggest that incubation could be more costly as a result of high resting costs. We highlight the importance of accounting for both active and resting costs when assessing energy expenditure.
Assuntos
Charadriiformes , Acelerometria , Animais , Regiões Árticas , Aves/fisiologia , Charadriiformes/fisiologia , Metabolismo Energético/fisiologiaRESUMO
Predicting the impacts of changing environments on phenotypes in wild populations remains a challenge. Growth, a trait that frequently influences fitness, is difficult to study as it is influenced by many environmental variables. To address this, we used a sliding window approach to determine the time windows when sea-surface and air temperatures have the potential to affect growth of black-legged kittiwakes (Rissa tridactyla) on a colony in the Northeast Pacific. We examined environmental drivers influencing nestling growth using data from a long-term (21-year) study, that food supplements a portion of the colony. The associations between kittiwake growth and climatic conditions in our study indicated that warmer environmental conditions can both positively and negatively impact nestling growth parameters depending on hatching order. We found that first-hatched nestlings had a heavier maximum mass under warm air temperatures and cold sea conditions. Warmer air temperatures negatively affected the second-hatched nestling in a brood. However, when air temperatures were warm, warmer sea-surface temperatures predicted heavy, fast-growing second-hatched nestlings in contrast to what we observed for first-hatched nestlings. Food supplementation alleviated the temperature effects, and competition among nestlings influenced how strongly a variable affected growth. We identified windows that might indicate specific biological pathways through which environmental variation affected growth directly or indirectly. Overall, our windows suggest that nestlings in shared nests will be most affected by warming conditions.
Assuntos
Charadriiformes , Animais , Alimentos , Estações do AnoRESUMO
Breeding animals trade off maximizing energy output to increase their number of offspring with conserving energy to ensure their own survival, leading to an energetic ceiling influenced by external, environmental factors or by internal, physiological factors. We examined whether internal or external factors limited energy expenditure by supplementally feeding breeding black-legged kittiwakes varying in individual quality, based on earlier work that defined late breeders as low-quality and early breeders as high-quality individuals. We tested whether energy expenditure increased when food availability decreased in both low- and high-quality birds; we predicted this would only occur in high-quality individuals capable of sustaining high levels of energy expenditure. Here, we find that food-supplemented birds expended less energy than control birds because they spent more time at the colony. However, foraging trips of food-supplemented birds were only slightly shorter than control birds, implying that food-supplemented birds were limited by food availability at sea similarly to control birds. Late breeders expended less energy, suggesting that low-quality individuals may not intake the energy necessary for sustaining high-energy output. Food-supplemented birds had more offspring than control birds, but offspring number did not influence energy expenditure, supporting the idea that the birds reached an energy ceiling. Males and lighter birds expended more energy, possibly compensating for relatively higher energy intake. Chick-rearing birds were working near their maximum, with highest levels of expenditure for early-laying (high-quality) individuals foraging at sea. Due to fluctuating marine environments, kittiwakes may be forced to change their foraging behaviors to maintain the balance between reproduction and survival.
Assuntos
Charadriiformes , Reprodução , Animais , Aves/fisiologia , Charadriiformes/fisiologia , Metabolismo Energético/fisiologia , Abastecimento de Alimentos , Masculino , Reprodução/fisiologiaRESUMO
During the Pacific marine heatwave of 2014-2016, abundance and quality of several key forage fish species in the Gulf of Alaska were simultaneously reduced throughout the system. Capelin (Mallotus catervarius), sand lance (Ammodytes personatus), and herring (Clupea pallasii) populations were at historically low levels, and within this community abrupt declines in portfolio effects identify trophic instability at the onset of the heatwave. Although compensatory changes in age structure, size, growth or energy content of forage fish were observed to varying degrees among all these forage fish, none were able to fully mitigate adverse impacts of the heatwave, which likely included both top-down and bottom-up forcing. Notably, changes to the demographic structure of forage fish suggested size-selective removals typical of top-down regulation. At the same time, changes in zooplankton communities may have driven bottom-up regulation as copepod community structure shifted toward smaller, warm water species, and euphausiid biomass was reduced owing to the loss of cold-water species. Mediated by these impacts on the forage fish community, an unprecedented disruption of the normal pelagic food web was signaled by higher trophic level disruptions during 2015-2016, when seabirds, marine mammals, and groundfish experienced shifts in distribution, mass mortalities, and reproductive failures. Unlike decadal-scale variability underlying ecosystem regime shifts, the heatwave appeared to temporarily overwhelm the ability of the forage fish community to buffer against changes imposed by warm water anomalies, thereby eliminating any ecological advantages that may have accrued from having a suite of coexisting forage species with differing life-history compensations.
Assuntos
Ecossistema , Peixes , Alaska , Animais , Cadeia Alimentar , ZooplânctonRESUMO
Current food supply is a major driver of timing of breeding in income-breeding animals, likely because increased net energy balance directly increases reproductive hormones and advances breeding. In capital breeders, increased net energy balance increases energy reserves, which eventually leads to improved reproductive readiness and earlier breeding. To test the hypothesis that phenology of income-breeding birds is independent of energy reserves, we conducted an experiment on food-supplemented ("fed") and control female black-legged kittiwakes (Rissa tridactyla). We temporarily increased energy costs (via weight handicap) in a 2 × 2 design (fed/unfed; handicapped/unhandicapped) during the pre-laying period and observed movement via GPS-accelerometry. We measured body mass, baseline hormones (corticosterone; luteinising hormone) before and after handicap manipulation, and conducted a gonadotropin-releasing hormone challenge. Females from all treatment groups foraged in similar areas, implying that individuals could adjust time spent foraging, but had low flexibility to adjust foraging distance. Consistent with the idea that income breeders do not accumulate reserves in response to increased food supply, fed birds remained within an energy ceiling by reducing time foraging instead of increasing energy reserves. Moreover, body mass remained constant until the onset of follicle development 20 days prior to laying regardless of feeding or handicap, implying that females were using a 'lean and fit' approach to body mass rather than accumulating lipid reserves for breeding. Increased food supply advanced endocrine and laying phenology and altered interactions between the hypothalamic-pituitary-adrenal axis and the hypothalamic-pituitary-gonadal axis, but higher energy costs (handicap) had little effect. Consistent with our hypothesis, increased food supply (but not net energy balance) advanced endocrine and laying phenology in income-breeding birds without any impact on energy reserves.
Assuntos
Charadriiformes/fisiologia , Abastecimento de Alimentos , Hormônios Gonadais/metabolismo , Comportamento Sexual Animal/fisiologia , Animais , Aves/fisiologia , Composição Corporal , Corticosterona/metabolismo , Metabolismo Energético/fisiologia , Comportamento Exploratório/fisiologia , Comportamento Alimentar/fisiologia , Feminino , Alimentos , Hormônio Liberador de Gonadotropina/metabolismo , Sistema Hipotálamo-Hipofisário/metabolismo , Hormônio Luteinizante/metabolismo , Masculino , Sistema Hipófise-Suprarrenal/metabolismo , Reprodução/fisiologia , Fatores de TempoRESUMO
Genes of the major histocompatibility complex (MHC) play a pivotal role in parasite resistance, and their allelic diversity has been associated with fitness variations in several taxa. However, studies report inconsistencies in the direction of this association, with either positive, quadratic or no association being described. These discrepancies may arise because the fitness costs and benefits of MHC diversity differ among individuals depending on their exposure and immune responses to parasites. Here, we investigated in black-legged kittiwake (Rissa tridactyla) chicks whether associations between MHC class-II diversity and fitness vary with sex and hatching order. MHC-II diversity was positively associated with growth and tick clearance in female chicks, but not in male chicks. Our data also revealed a positive association between MHC-II diversity and survival in second-hatched female chicks (two eggs being the typical clutch size). These findings may result from condition-dependent parasite infections differentially impacting sexes in relation to hatching order. We thus suggest that it may be important to account for individual heterogeneities in traits that potentially exert selective pressures on MHC diversity in order to properly predict MHC-fitness associations.
Assuntos
Charadriiformes , Parasitos , Alelos , Animais , Charadriiformes/genética , Feminino , Variação Genética , Antígenos de Histocompatibilidade Classe II/genética , Complexo Principal de Histocompatibilidade/genética , Masculino , Seleção GenéticaRESUMO
Muscle ultrastructure is closely linked with athletic performance in humans and lab animals, and presumably plays an important role in the movement ecology of wild animals. Movement is critical for wild animals to forage, escape predators and reproduce. However, little evidence directly links muscle condition to locomotion in the wild. We used GPS-accelerometers to examine flight behaviour and muscle biopsies to assess muscle ultrastructure in breeding black-legged kittiwakes (Rissa tridactyla). Biopsied kittiwakes showed similar reproductive success and subsequent over-winter survival to non-biopsied kittiwakes, suggesting that our study method did not greatly impact foraging ability. Muscle fibre diameter was negatively associated with wing beat frequency, likely because larger muscle fibres facilitate powered flight. The number of nuclei per fibre was positively associated with average air speed, likely because higher power output needed by faster-flying birds required plasticity for muscle fibre recruitment. These results suggest the potential for flight behaviour to predict muscle ultrastructure.
Assuntos
Animais Selvagens , Charadriiformes , Acelerometria , Animais , Aves , Voo Animal , Humanos , Fibras Musculares EsqueléticasRESUMO
Individual condition at one stage of the annual cycle is expected to influence behaviour during subsequent stages, yet experimental evidence of food-mediated carry-over effects is scarce. We used a food supplementation experiment to test the effects of food supply during the breeding season on migration phenology and non-breeding behaviour. We provided an unlimited supply of fish to black-legged kittiwakes (Rissa tridactyla) during their breeding season on Middleton Island, Alaska, monitored reproductive phenology and breeding success, and used light-level geolocation to observe non-breeding behaviour. Among successful breeders, fed kittiwakes departed the colony earlier than unfed controls. Fed kittiwakes travelled less than controls during the breeding season, contracting their non-breeding range. Our results demonstrate that food supply during the breeding season affects non-breeding phenology, movement and distribution, providing a potential behavioural mechanism underlying observed survival costs of reproduction.
Assuntos
Charadriiformes , Alaska , Animais , Cruzamento , Abastecimento de Alimentos , Ilhas , Reprodução , Estações do AnoRESUMO
Carotenoid-based ornaments are common signaling features in animals. Although the mechanisms that link color-based signals to individual condition is key to understanding the evolution and function of these ornaments, they are most often poorly known. Several hypotheses have been posited. They include: (i) the role of foraging abilities on carotenoid acquisition and thereby carotenoid-based ornaments, and (ii) the role of internal processes linked to individual quality on the allocation and conversion of carotenoids in integuments. Here, we tested the influence of dietary carotenoid access versus internal process on gape coloration in black-legged kittiwakes (Rissa tridactyla). This seabird displays a vibrant red gape, whose coloration varies with individual quality in males and is due to the deposition of red ketocarotenoids, such as astaxanthin. We decreased hydroxycarotenoid and ketocarotenoid levels in plasma, but increased efficiency in internal processes linked to nutritional condition, by supplementing breeding males with capelin, a natural energy-rich fish prey. We found that, despite having lower carotenoid levels in plasma, supplemented birds developed redder coloration than control birds, but only in the year when dietary levels of astaxanthin in the natural diet were low. In contrast, in the astaxanthin-rich year, supplemented males had a less-red gape than unsupplemented birds. These results suggest that inter-individual differences in internal processes may be sufficient to maintain the honesty of gape coloration under conditions of low dietary astaxanthin levels. Nonetheless, when inter-individual variations in dietary astaxanthin levels are elevated (such as in the crustacean-rich year), carotenoid access seems a more limiting factor to the expression of gape coloration than internal processes. Therefore, our study revealed a complex mechanism of gape color production in kittiwakes, and suggests that the main factor maintaining the condition dependency of this ornaments may vary with environmental conditions and diet composition.
Assuntos
Charadriiformes/fisiologia , Cor , Dieta , Estado Nutricional , Ração Animal/análise , Animais , Suplementos Nutricionais/análise , Masculino , Pigmentação , Xantofilas/administração & dosagem , Xantofilas/metabolismoRESUMO
Early-life conditions can drive ageing patterns and life history strategies throughout the lifespan. Certain social, genetic and nutritional developmental conditions are more likely to produce high-quality offspring: those with good likelihood of recruitment and productivity. Here, we call such conditions "favoured states" and explore their relationship with physiological variables during development in a long-lived seabird, the black-legged kittiwake (Rissa tridactyla). Two favoured states were experimentally generated by manipulation of food availability and brood size, while hatching order and sex were also explored as naturally generating favoured states. Thus, the favoured states we explored were high food availability, lower levels of sibling competition, hatching first and male sex. We tested the effects of favoured developmental conditions on growth, stress, telomere length (a molecular marker associated with lifespan) and nestling survival. Generation of favoured states through manipulation of both the nutritional and social environments furthered our understanding of their relative contributions to development and phenotype: increased food availability led to larger body size, reduced stress and higher antioxidant status, while lower sibling competition (social environment) led to lower telomere loss and longer telomere lengths in fledglings. Telomere length predicted nestling survival, and wing growth was also positively correlated with telomere length, supporting the idea that telomeres may indicate individual quality, mediated by favoured states.
Assuntos
Tamanho Corporal , Charadriiformes/crescimento & desenvolvimento , Estresse Fisiológico , Telômero/ultraestrutura , Animais , Charadriiformes/genética , Tamanho da Ninhada , Feminino , Alimentos , Masculino , Fenótipo , Meio SocialRESUMO
The integral of the dynamic component of acceleration over time has been proposed as a measure of energy expenditure in wild animals. We tested that idea by attaching accelerometers to the tails of free-ranging pelagic cormorants (Phalacrocorax pelagicus) and simultaneously estimating energy expenditure using doubly labelled water. Two different formulations of dynamic body acceleration, [vectorial and overall DBA (VeDBA and ODBA)], correlated with mass-specific energy expenditure (both R(2)=0.91). VeDBA models combining and separately parameterizing flying, diving, activity on land and surface swimming were consistently considered more parsimonious than time budget models and showed less variability in model fit. Additionally, we observed evidence for the presence of hypometabolic processes (i.e. reduced heart rate and body temperature; shunting of blood away from non-essential organs) that suppressed metabolism in cormorants while diving, which was the most metabolically important activity. We concluded that a combination of VeDBA and physiological processes accurately measured energy expenditure for cormorants.
Assuntos
Aceleração , Aves/fisiologia , Ecossistema , Metabolismo Energético/fisiologia , Alaska , Animais , Peso Corporal , Deutério , Feminino , Ilhas , Masculino , Isótopos de Oxigênio , Água/químicaRESUMO
Flight is a key adaptive trait. Despite its advantages, flight has been lost in several groups of birds, notably among seabirds, where flightlessness has evolved independently in at least five lineages. One hypothesis for the loss of flight among seabirds is that animals moving between different media face tradeoffs between maximizing function in one medium relative to the other. In particular, biomechanical models of energy costs during flying and diving suggest that a wing designed for optimal diving performance should lead to enormous energy costs when flying in air. Costs of flying and diving have been measured in free-living animals that use their wings to fly or to propel their dives, but not both. Animals that both fly and dive might approach the functional boundary between flight and nonflight. We show that flight costs for thick-billed murres (Uria lomvia), which are wing-propelled divers, and pelagic cormorants (Phalacrocorax pelagicus) (foot-propelled divers), are the highest recorded for vertebrates. Dive costs are high for cormorants and low for murres, but the latter are still higher than for flightless wing-propelled diving birds (penguins). For murres, flight costs were higher than predicted from biomechanical modeling, and the oxygen consumption rate during dives decreased with depth at a faster rate than estimated biomechanical costs. These results strongly support the hypothesis that function constrains form in diving birds, and that optimizing wing shape and form for wing-propelled diving leads to such high flight costs that flying ceases to be an option in larger wing-propelled diving seabirds, including penguins.
Assuntos
Evolução Biológica , Charadriiformes/fisiologia , Mergulho/fisiologia , Voo Animal/fisiologia , Modelos Biológicos , Spheniscidae/fisiologia , Alaska , Animais , Fenômenos Biomecânicos , Metabolismo Energético/fisiologia , Nunavut , Análise de Regressão , Especificidade da EspécieRESUMO
Supplementation of food to wild animals is extensively applied as a conservation tool to increase local production of young. However, in long-lived migratory animals, the carry-over effects of food supplementation early in life on the subsequent recruitment of individuals into natal populations and their lifetime reproductive success are largely unknown. We examine how experimental food supplementation early in life affects: (i) recruitment as breeders of kittiwakes Rissa tridactyla born in a colony on Middleton Island (Alaska) between 1996 and 2006 (n = 1629) that bred in the same colony through 2013 (n = 235); and (ii) breeding success of individuals that have completed their life cycle at the colony (n = 56). Birds were raised in nests that were either supplemented with food (Fed) or unsupplemented (Unfed). Fledging success was higher in Fed compared with Unfed nests. After accounting for hatching rank, growth and oceanic conditions at fledging, Fed fledglings had a lower probability of recruiting as breeders in the Middleton colony than Unfed birds. The per-nest contribution of breeders was still significantly higher for Fed nests because of their higher productivity. Lifetime reproductive success of a subset of kittiwakes that thus far had completed their life cycle was not affected by the food supplementation during development. Our results cast light on the carry-over effects of early food conditions on the vital rates of long-lived animals and support food supplementation as an effective conservation strategy for long-lived seabirds.
Assuntos
Charadriiformes/fisiologia , Suplementos Nutricionais/análise , Comportamento de Nidação , Reprodução , Alaska , Animais , Conservação dos Recursos Naturais , Dieta , Feminino , MasculinoRESUMO
The reproductive success of wild animals usually increases with age before declining at the end of life, but the proximate mechanisms underlying those patterns remain elusive. Young animals are expected to invest less in current reproduction due to high prospects for future reproduction (the "restraint" hypothesis). The oldest animals may also show restraint when conditions are sub-optimal where even a small increase in reproductive investment may lead to death ("terminal restraint"). Alternatively, reproduction may be constrained by lack of experience and senescence (the "constraint" hypothesis). In two species of breeding seabirds, behavioural (time to return the offspring, calmness during restraint) and physiological (metabolism, glucose and corticosterone) parameters responded similarly to stress with advancing age, implying a generalized stress response. Across those parameters, birds were "shy" (high stress response) when young or old, and "bold" (low stress response) when middle-aged. Specifically, free corticosterone, the principal avian glucocorticoid responsible for directing energy away from reproduction and towards immediate survival following stress, was highest in both young and very old stressed birds. All age groups had a similar adrenal capacity to produce corticosterone, implying that middle-aged birds were showing restraint. Because the stress response, was highest at ages when the probability of current reproduction was lowest rather than at ages when the probability of future reproduction was highest we concluded that birds restrained reproductive investment based on current conditions rather than potential future opportunities. In particular, old birds showed terminal restraint when stressed. Hormonal cues promoted investment in adult survival over reproductive output at both the start and end of life consistent with the restraint hypothesis.
Assuntos
Adaptação Psicológica/fisiologia , Envelhecimento/fisiologia , Charadriiformes/fisiologia , Comportamento de Nidação/fisiologia , Reprodução/fisiologia , Adaptação Psicológica/efeitos dos fármacos , Animais , Comportamento Animal/efeitos dos fármacos , Corticosterona/metabolismo , Dexametasona/farmacologia , Feminino , Longevidade/fisiologia , Masculino , Comportamento de Nidação/efeitos dos fármacos , Reprodução/efeitos dos fármacos , Estresse Psicológico/etiologia , Estresse Psicológico/fisiopatologiaRESUMO
In nest-bound avian offspring, food shortages typically trigger a release of the stress hormone corticosterone (CORT). Recent studies indicate that CORT is passively deposited in the tissue of growing feathers and thus may provide an integrated measure of stress incurred during development in the nest. The current hypothesis predicts that, assuming a constant rate of feather growth, elevated CORT circulating in the blood corresponds to higher levels of CORT in feather tissue, but experimental evidence for nutritionally stressed chicks is lacking. Here, we examined how food limitation affects feather CORT content in the rhinoceros auklet (Cerorhinca moncerata). We (i) used captive chicks reared on control versus restricted diets, and (ii) applied this technique to free-living chicks with unknown nutritional histories that fledged at three separate colonies. We found that (i) feather growth was not affected by experimentally induced nutritional stress; (ii) captive chicks raised on a restricted diet had higher levels of CORT in their primary feathers; (iii) feather CORT deposition is a sensitive method of detecting nutritional stress; and (iv) free-living fledglings from the colony with poor reproductive performance had higher CORT in their primary feathers. We conclude that feather CORT is a sensitive integrated measure revealing the temporal dynamics of food limitations experienced by rhinoceros auklet nestlings. The use of feather CORT may be a powerful endocrine tool in ecological and evolutionary studies of bird species with similar preferential allocation of limited resources to feather development.
Assuntos
Charadriiformes/fisiologia , Corticosterona/metabolismo , Dieta , Plumas/química , Alaska , Animais , Charadriiformes/crescimento & desenvolvimento , Corticosterona/sangue , Meio Ambiente , Japão , Reprodução , Estresse FisiológicoRESUMO
Nest-bound chicks depend entirely on their parents for food, often leading to high sibling competition. Asynchronous hatching, resulting from the onset of incubation before clutch completion, facilitates the establishment of within-nest hierarchy, with younger chicks being subject to lower feeding and growth rates. Because social and nutritional stresses affect baseline stress hormone levels in birds, younger chicks are expected to have higher levels of corticosterone than their siblings. As previous studies showed that hatching asynchrony magnitude influences the course of sibling competition, it should also affect baseline corticosterone. We measured baseline corticosterone at age 5 days in nestling black-legged kittiwakes (Rissa tridactyla) in 3 types of experimental broods: synchronous, asynchronous, and highly asynchronous. Sexual dimorphism takes place during chick-rearing and might also influence baseline corticosterone, we thus included chick sex in our analyses and also monitored chick growth and survival. Baseline corticosterone did not differ among A-chicks, but was higher in B-chicks from highly asynchronous broods compared with the other brood types, in line with the presumed increase in nutritional stress. In asynchronous broods, A-chicks had higher baseline corticosterone than their siblings, contrary to our expectations. We interpret that result as a cost of dominance among A-chicks. In line with previous studies, mass gain was negatively correlated with baseline corticosterone levels. We found that baseline corticosterone predicted survival in a sex-specific way. Regardless of hatching rank, males with higher baseline corticosterone suffered higher mortality, suggesting that males were more sensitive to high level of stress, independently of its cause.
Assuntos
Cruzamento , Charadriiformes/fisiologia , Comportamento de Nidação/fisiologia , Estresse Fisiológico/fisiologia , Animais , Corticosterona/sangue , Feminino , MasculinoRESUMO
Because of ongoing rapid climate change, many ecosystems are becoming both warmer and more variable, and these changes are likely to alter the magnitude and variability of natural selection acting on wild populations. Critically, changes and fluctuations in selection can impact both population demography and evolutionary change. Therefore, predicting the impacts of climate change depends on understanding the magnitude and variation in selection on traits across different life stages and environments. Long-term experiments in wild settings are a great opportunity to determine the impact of environmental conditions on selection. Here we examined variability in the strength of selection on size traits of nestling black-legged kittiwakes (Rissa tridactyla) in a 25-year study including a food supplementation experiment on Middleton Island in the Gulf of Alaska. Using mixed effect models, we examined the annual variability of stage-specific and resource-specific selection gradients across 25 years. We found that (a) larger and heavier hatchlings were the most likely to survive during early ontogeny, (b) non-food supplemented younger nestlings in a brood experienced the strongest selection, and (c) warmer conditions increased the magnitude of selection on nestling mass and affected non-food supplemented and second-hatched nestlings the most. Our results suggested that variable resource dynamics likely caused some of the changes in selection from year to year and that warming conditions increased the strength of selection on subarctic seabird growth. However, our experimental manipulation revealed that local environmental heterogeneity could buffer the selection expected from broader climatic changes. Consequently, understanding the interactive effects of local conditions and general changes in climate seems likely to improve our ability to predict future selection gradients.