Diel variations in the estimated refractive index of bulk oceanic particles.

The index of refraction (n) of particles is an important parameter in optical models that aims to extract particle size and carbon concentrations from light scattering measurements. An inadequate choice of n can critically affect the characterization and interpretation of optically-derived parameters, including those from satellite-based models which provide the current view of how biogeochemical processes vary over the global ocean. Yet, little is known about how n varies over time and space to inform such models. Particularly, in situ estimates of n for bulk water samples and at diel-resolving time scales are rare. Here, we demonstrate a method to estimate n using simultaneously and independently collected particulate beam attenuation coefficients, particle size distribution data, and a Mie theory model. We apply this method to surface waters of the North Pacific Subtropical Gyre (NPSG) at hourly resolution. Clear diel cycles in n were observed, marked by minima around local sunrise and maxima around sunset, qualitatively consistent with several laboratory-based estimates of n for specific phytoplankton species. A sensitivity analysis showed that the daily oscillation in n amplitude was somewhat insensitive to broad variations in method assumptions, ranging from 11.3 ± 4.3% to 16.9 ± 2.9%. Such estimates are crucial for improvement of algorithms that extract the particle size and production from bulk optical measurements, and could potentially help establish a link between n variations and changes in cellular composition of in situ particles.

Diel variability of bulk optical properties associated with the growth and division of small phytoplankton in the North Pacific Subtropical Gyre.

Cross-platform observing systems are requisite to capturing the temporal and spatial dynamics of particles in the ocean. We present simultaneous observations of bulk optical properties, including the particulate beam attenuation (cp) and backscattering (bbp) coefficients, and particle size distributions collected in the North Pacific Subtropical Gyre. Clear and coherent diel cycles are observed in all bulk and size-fractionated optical proxies for particle biomass. We show evidence linking diurnal increases in cp and bbp to daytime particle growth and division of cells, with particles <7µm driving the daily cycle of particle production and loss within the mixed layer. Flow cytometry data reveal the nitrogen-fixing cyanobacterium Crocosphaera (∼4-7µm) to be an important driver of cp at the time of sampling, whereas Prochlorococcus dynamics (∼0.5µm) were essential to reproducing temporal variability in bbp. This study is a step towards improved characterization of the particle size range represented by in situ bulk optical properties and a better understanding of the mechanisms that drive variability in particle production in the oligotrophic open ocean.