Spatially resolved analysis of variation in barley (Hordeum vulgare) grain micronutrient accumulation.

Genetic biofortification requires knowledge on natural variation and the underlying mechanisms of micronutrient accumulation. We therefore studied diversity in grain micronutrient concentrations and spatial distribution in barley (Hordeum vulgare), a genetically tractable model cereal and an important crop with widespread cultivation. We assembled a diverse collection of barley cultivars and landraces and analysed grain micronutrient profiles in genebank material and after three independent cultivations. Lines with contrasting grain zinc (Zn) accumulation were selected for in-depth analysis of micronutrient distribution within the grain by micro-proton-induced X-ray emission (µ-PIXE). Also, we addressed association with grain cadmium (Cd) accumulation. The analysis of > 120 lines revealed substantial variation, especially in grain Zn concentrations. A large fraction of this variation is due to genetic differences. Grain dissection and µ-PIXE analysis of contrasting lines showed that differences in grain Zn accumulation apply to all parts of the grain including the endosperm. Cd concentrations exceeded the Codex Alimentarius threshold in most of the representative barley lines after cultivation in a Cd-contaminated agricultural soil. Two important conclusions for biofortification are: first, high-Zn grains contain more Zn also in the consumed parts of the grain; and second, higher micronutrient concentrations are strongly associated with higher Cd accumulation.

[Wind turbine and infrasound: No evidence for health-related impairment - a physical, medical and social report]. / Windenergieanlagen und Infraschall: Keine Evidenz für gesundheitliche Beeinträchtigungen ­ eine physikalische, medizinische und gesellschaftliche Einordnung.

Based on the recent discussion about health impacts of wind turbine noise and infrasound emissions, we present the physical facts and summarize related studies in a narrative review. We are exposed to infrasound emissions from different sources, where wind turbine farms do not cause particularly high infrasound emissions. Epidemiological studies found no association between wind turbine farms and the incidence of diabetes mellitus, heart attacks, strokes and medication with antihypertensive drugs, but a more frequent prescription of sleep medication. In contrast, key indicators of objective sleep outcomes are not impacted by wind turbine noise. Health complaints are more frequently proven, if anti-wind-turbine-groups were active, which is consistent with the psychogenic hypotheses with nocebo effects likely play an important role. Without evidence of health impacts from wind turbine farms, an ongoing slowdown in the urgently needed expansion of renewable energies is not justifiable.