LncRNA AFAP1-AS1 Promotes the Progression of Colorectal Cancer through miR-195-5p and WISP1.

OBJECTIVE: Colorectal cancer (CRC) is the most common cancer. But, the molecular mechanisms of CRC progression are not fully understood. This study was conducted to explore how the long noncoding RNA actin filament-associated protein 1-antisense RNA1 (lncRNA AFAP1-AS1) participates in CRC progression through the regulation of microRNA-195-5p (miR-195-5p) and wingless-type inducible signaling pathway protein-1 (WISP1). METHODS: The expressions of AFAP1-AS1, miR-195-5p, and WISP1 were detected by RT-qPCR or western blot. A dual-luciferase assay confirmed the target relationship of AFAP1-AS1, miR-195-5p, and WISP1. Colony formation, wound-healing, and Transwell assays were used to detect the growth, migration, and invasion abilities of cells, respectively. RESULTS: AFAP1-AS1 and WISP1 expressions were notably increased, and miR-195-5p expression was markedly reduced in CRC. The dual-luciferase assay verified that AFAP1-AS1 could bind to miR-195-5p. AFAP1-AS1 knockdown could inhibit the malignant behavior of CRC cells. miR-195-5p could target and regulate WISP1 expression. Overexpression of WISP1 or miR-195-5p inhibition reversed the inhibition effect of AFAP1-AS1 knockdown on the biological activity of CRC cells. CONCLUSIONS: AFAP1-AS1 knockdown may inhibit the proliferation, migration, and invasion of CRC cells through the miR-195-5p/WISP1 axis.

Three-dimensional pore characterization of intact loess and compacted loess with micron scale computed tomography and mercury intrusion porosimetry.

The pore structure is one of the most important properties of soil, which can directly affect the other properties such as water content, permeability and strength. It is of great significance to study the soil pore structure for agricultural cultivation, water and soil conservation and engineering construction. This paper investigates the 3D pore characterization of intact loess and four kinds of compacted loess (with different dry density) in northwest China. Micro scale computed tomography and mercury intrusion porosimetry tests were performed to get the porosity, specific surface area, pore size distribution, connected pores content and isolated pores content of different samples. Results show that the intact loess has more connected pores than the compacted loess, and the compacted loess whose dry density appears to be modelled well still have different pore structure with the intact loess. In addition, as the compactness increasing, the large pores (>13 µm) were firstly broken into medium pores (8~13 µm) and some small pores (<8 µm) until the pore structure was close to the natural structure of the intact loess, after that medium pores began to be broken into small pores.