Demineralization inhibition of direct tooth-colored restorative materials.

This study compared the demineralization inhibition properties of fluoride releasing tooth-colored restorative materials. Materials evaluated included a giomer (Reactmer, Shofu [RM]), a conventional glass ionomer (Fuji II, GC [FJ]), a resin modified glass ionomer (Fuji II LC, GC [FL]) and a compomer (Dyract AP, Dentsply [DY]). A non-fluoride releasing composite (Spectrum TPH, Dentsply [SP]) was used for comparison. Class V preparations on buccal and palatal/lingual were made at the CEJ of 75 freshly extracted molars. The teeth were randomly divided into five groups of 15 and restored with the various materials. The occlusal half of each restoration was in enamel, while the gingival half was in dentin. The restored teeth were stored in distilled water at 37 degrees C for two weeks and subjected to artificial caries challenge (18 hours demineralization [pH 5.0] followed by six hours of remineralization [pH 7.0]) for three days. Sections of 130 +/- 20 microm were examined with a polarized light microscope, and outer lesion depth [OLD] and wall area [WA] lesion/inhibition measurements were made using image analysis software. All data were subjected to statistical analyses at 0.05 significance level. For the various materials, OLD ranged from 54.55 to 65.86 microm and 124.68 to 145.97 microm in enamel and dentin, respectively. WA ranged from -2356.13 to 1398.20 microm2 and -3011.73 to 5095.80 microm2 (positive values indicate wall inhibition, negative values indicate wall lesion) in enamel and dentin, respectively. Results of ANOVA/Scheffe's post-hoc test (p<0.05) were as follows: Enamel OLD--no significant difference between materials; Dentin OLD--SP > FJ, FL & RM; Enamel WA inhibition--FJ, FL & RM > DY & SP and Dentin WA inhibition--FJ > FL > RM > DY > SP. The demineralization inhibition effect of giomers, conventional and resin-modified glass ionomer cements appear to be more evident at the margins of restorations.

Effect of collection tube type and preanalytical handling on myeloperoxidase concentrations.

BACKGROUND: Myeloperoxidase (MPO) has shown potential as a marker for cardiovascular disease. Limited studies have been published with a variety of sample types, resulting in a wide range of MPO values. Little is known or understood about the impact of collection tube type and preanalytical handling of specimens for MPO determination. METHOD: MPO concentration was determined by use of the ARCHITECT(R) MPO research use assay, which is currently under development. Samples were collected into multiple anticoagulant collection tubes from donors and patients presenting to the emergency department with symptoms of acute coronary syndromes. Whole blood was stored on ice or at room temperature for predetermined time periods. We also evaluated serum and plasma after centrifugation followed by storage at room temperature, 2-8 degrees C, and below -10 degrees C. RESULTS: Baseline sample concentrations were dependent on collection tube type as well as handling conditions. MPO concentrations were consistently higher in samples collected in serum and heparin plasma tubes than in samples in EDTA or citrate tubes. Spike recovery was acceptable in all sera and plasma tested, indicating that the increased MPO concentrations were not due directly to an anticoagulant interference. CONCLUSIONS: The collection tube type and preanalytical handling are critical for accurate and consistent MPO measurement. The preferred anticoagulant and tubes are the EDTA or EDTA plasma preparation tube. MPO concentrations in samples collected in these tubes are stable before centrifugation as whole blood as well as plasma after processing.