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Exp Dermatol ; 15(6): 421-31, 2006 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-16689858

RESUMO

The accelerated migration of Langerhans cells (LCs) out of the epidermis and up-regulation of maturation markers, upon treatment with subtoxic concentrations of chemicals, were used as the criteria to determine the potential of allergenic chemicals capable of inducing a hapten-specific delayed-type hypersensitivity reaction. Here we report the findings of a study in which seven chemicals, coded and tested in a blind fashion, were classified as contact allergens or non-allergens using the human organotypic skin explant culture (hOSEC) model. All chemicals that were identified as a contact sensitizer on decoding induced a definite decrease in the number of CD1a and HLA-DR-positive epidermal LCs in the epidermis of the skin explants, as determined by both semiquantitative immunohistochemistry and quantitative flow cytometric analysis. A significant increase in the number of CD83(+) cells was accompanied by up-regulation of activation molecules in the epidermis of hOSEC exposed specifically to contact allergens. In contrast, there were only minor alterations in epidermal LC numbers, expression of CD83 and other activation markers by LCs when the biopsies were treated with non-toxic concentrations of non-allergenic irritants and vehicles. The data suggest that an increased epidermal LC migration and maturation accompanied by increased expression of activation markers could be used as end-point determinants to screen allergens in a non-animal alternative hOSEC model.


Assuntos
Alérgenos/toxicidade , Haptenos/toxicidade , Células de Langerhans/efeitos dos fármacos , Pele/efeitos dos fármacos , Alérgenos/administração & dosagem , Antígenos CD/metabolismo , Diferenciação Celular/efeitos dos fármacos , Movimento Celular/efeitos dos fármacos , Dermatite Alérgica de Contato/etiologia , Dermatite Alérgica de Contato/imunologia , Dermatite Alérgica de Contato/patologia , Haptenos/administração & dosagem , Humanos , Imunoglobulinas/metabolismo , Células de Langerhans/patologia , Células de Langerhans/fisiologia , Glicoproteínas de Membrana/metabolismo , Modelos Biológicos , Pele/patologia , Pele/fisiopatologia , Técnicas de Cultura de Tecidos , Antígeno CD83
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