RESUMO
To explore the molecular mechanisms of lipid accumulation in different types of adipose tissue, the transcriptomes of the subcutaneous and intramuscular adipose tissues from the Large White pig were determined using RNA-seq technology and bioinformatics methods. The differential gene expression profiles were identified and analyzed with the Gene Ontology, KEGG pathway and protein-protein interaction network strategies. There were 180 differentially expressed genes between the two adipose tissues. The genes up-regulated in the subcutaneous, as compared to intramuscular, adipose tissues were mainly involved in the biological processes related to lipid metabolism. The down-regulated genes were significantly enriched in MAPK signaling pathway, suggesting that this signaling pathway could have an important regulatory role(s) in adipocyte differentiation. In summary, differentially expressed genes between the subcutaneous and intramuscular adipose tissues were predominantly involved in lipid metabolism/accumulation and regulation of adipogenesis in the Large White pig.
Assuntos
Tecido Adiposo/metabolismo , Expressão Gênica/genética , Músculos/metabolismo , Pele/metabolismo , Suínos/genética , Suínos/metabolismo , Adipócitos/metabolismo , Adipogenia/genética , Animais , Diferenciação Celular/genética , Biologia Computacional/métodos , Regulação para Baixo/genética , Feminino , Ontologia Genética , Análise de Sequência de RNA/métodos , Transdução de Sinais/genéticaRESUMO
Hog deer (Axis porcinus) is a small deer species in family Cervidae and has been undergoing a serious and global decline during the past decades. Chengdu Zoo currently holds a captive population of hog deer with sufficient genetic diversity in China. We sequenced and de novo assembled its genome sequence in the present study. A total of six different insert-size libraries were sequenced and generated 395 Gb of clean data in total. With aid of the linked reads of 10X Genomics, genome sequence was assembled to 2.72 Gb in length (contig N50, 66.04 Kb; scaffold N50, 20.55 Mb), in which 94.5% of expected genes were detected. We comprehensively annotated 22,473 protein-coding genes, 37,019 tRNAs, and 1,058 Mb repeated sequences. The newly generated reference genome is expected to significantly contribute to comparative analysis of genome biology and evolution within family Cervidae.