RESUMO
Studies of the HEDGEHOG signalling pathway in Drosophila have revealed a functional link between two genes, cubitus interruptus and patched, whose human homologues are, respectively, a proto-oncogene and a tumour suppressor. While the former has been implicated as a transcription factor, controversy has surrounded the function of the transmembrane protein encoded by the latter. Somewhere in the signal-transduction pathway between these two lies protein kinase A (PKA), and now SMOOTHENED, whose similarity to G-protein-coupled receptors suggests a link with PKA, has also been implicated in the pathway. This article summarizes the current understanding of the pathway and the interactions between these proteins.
RESUMO
Hedgehog proteins constitute one of the major classes of intercellular signals that control inductive interactions during animal development. These proteins undergo unusual lipid modifications and signal through an unconventional transmembrane protein receptor that is characterized by a sequence motif implicated in sterol sensing. Recent studies suggest that the lipid adducts regulate the range and potency of the signals, whereas the sterol-sensing domain is essential for receptor activity.
Assuntos
Colesterol/metabolismo , Proteínas de Insetos/metabolismo , Metabolismo dos Lipídeos , Transdução de Sinais , Transativadores/metabolismo , Animais , Proteínas de Drosophila/química , Proteínas de Drosophila/genética , Proteínas de Drosophila/metabolismo , Proteínas Hedgehog , Humanos , Proteínas de Insetos/química , Proteínas de Insetos/genética , Ligação Proteica , Processamento de Proteína Pós-Traducional , Transporte Proteico , Transativadores/química , Transativadores/genéticaRESUMO
The formation of complex structures in any multicellular organism requires cooperative behaviour within cell populations. Genetic and molecular analysis of pattern formation in the Drosophila embryo is providing us with new insights into the cellular basis of this process, implicating a diversity of molecules, some familiar, others novel, in intercellular communication.
Assuntos
Drosophila/embriologia , Embrião não Mamífero/ultraestrutura , Regulação da Expressão Gênica , Genes Reguladores , Animais , Comunicação Celular , Drosophila/genética , Genes , Genes Homeobox , Larva/ultraestrutura , Morfogênese , Fenótipo , Proteínas/genética , Proteínas/fisiologiaRESUMO
The cloning of vertebrate homologues of the Drosophila segment polarity gene patched has led to confirmation of a role for the multipass transmembrane protein which it encodes as a receptor for secreted signalling proteins of the Hedgehog family. In addition, human patched has been identified as a tumour suppressor gene implicated in basal cell carcinomas and medullablastomas.
Assuntos
Carcinoma Basocelular/genética , Proteínas de Drosophila , Meduloblastoma/genética , Proteínas de Membrana/genética , Vertebrados/crescimento & desenvolvimento , Animais , Síndrome do Nevo Basocelular/genética , Proteínas Hedgehog , Humanos , Proteínas de Insetos/metabolismo , Mutação , Receptores Patched , Receptores de Superfície Celular , Transdução de SinaisRESUMO
Members of the hedgehog gene family encode a novel class of secreted proteins and are expressed in embryonic cells known to possess important signalling activities in organisms as diverse as flies and chickens. Proteins of the hedgehog family act in these different developmental contexts as both permissive and instructive signals. How this signalling activity is transduced is (as yet) poorly understood, but recent studies point to the involvement of protein kinase A in both Drosophila and vertebrates.
Assuntos
Drosophila/embriologia , Indução Embrionária , Proteínas/fisiologia , Transdução de Sinais/fisiologia , Transativadores , Vertebrados/embriologia , Animais , Desenvolvimento Embrionário e Fetal , Proteínas Hedgehog , Botões de Extremidades/embriologiaRESUMO
Recent findings indicate that the Drosophila gene hedgehog and its vertebrate homologues act in analogous ways as signalling molecules that help provide cells with positional information during development.
Assuntos
Drosophila/embriologia , Animais , Polaridade Celular/genética , Drosophila/genética , Genes de Insetos , Mutação , Transdução de Sinais/genéticaRESUMO
By coupling to cholesterol, Hedgehog can be anchored to the cells where it is made, yet to act as a morphogen, it must be able to move away from its source. Novel genes have now been identified that control the release and dispersal of Hedgehog, shedding new light on the part played by cholesterol in these processes.
Assuntos
Colesterol/fisiologia , Proteínas de Drosophila , Proteínas de Insetos/metabolismo , Transdução de Sinais , Animais , Ligação Competitiva , Drosophila/genética , Proteínas Hedgehog , Proteínas de Insetos/genética , Modelos Biológicos , Proteoglicanas/metabolismo , Asas de Animais/citologiaRESUMO
BACKGROUND: Members of the hedgehog (hh) gene family encode a novel class of proteins implicated in positional signalling in both invertebrates and vertebrates. In Drosophila, the hh gene has been shown to regulate patterning of the imaginal discs, the precursors of the insect limbs. In a remarkably similar fashion, the function and expression of the sonic hedgehog (shh) gene is closely associated with the 'zone of polarizing activity' (ZPA) that controls antero-posterior patterning of the vertebrate limb. Both of these functions suggest a role for hedgehog family proteins as morphogens. An alternative possibility, however, is that hh and its homologues act to control the expression of other instructive signalling molecules. RESULTS: We have explored this issue by examining the effects on Drosophila wing patterning of ectopically expressing varying levels of hh and shh, as well as of the putative hh target gene, decapentaplegic (dpp), a member of the transforming growth factor-beta family of signalling molecules. We find that different levels of hh activity can induce graded changes in the patterning of the wing, and that zebrafish shh acts in a similar though attenuated fashion. Varying levels of ectopic hh and shh activity can differentially activate transcription of the patched and dpp genes. Furthermore, ectopic expression of dpp alone is sufficient to induce the pattern alterations caused by ectopic hh or shh activity. CONCLUSION: Thus, hh family proteins can elicit different responses in a dose-dependent manner in the imaginal disc. The principal function of hh, however, is to activate transcription of dpp at the compartment boundary, thereby establishing a source of dpp activity that is the primary determinant of antero-posterior patterning.
Assuntos
Proteínas de Drosophila , Drosophila/embriologia , Hormônios de Inseto/fisiologia , Proteínas/fisiologia , Transativadores , Animais , Drosophila/fisiologia , Regulação da Expressão Gênica , Proteínas Hedgehog , Proteínas de Membrana/fisiologia , Receptores de Superfície Celular , Asas de Animais/fisiologia , Peixe-ZebraRESUMO
Recent studies have implicated the signaling factor Sonic hedgehog (Shh) as a negative regulator of pancreatic development, but as a positive regulator of pancreas function in amniotes [1-4]. Here, using genetic analysis, we show that specification of the pancreas in the teleost embryo requires the activity of Hh proteins. Zebrafish embryos compromised in Hh signaling exhibit disruption in the expression of the pancreas-specifying homeobox gene pdx-1 and concomitantly show almost complete absence of the endocrine pancreas. Reciprocally, ubiquitous activation of the Hh pathway in wild-type embryos causes ectopic induction of endodermal pdx-1 expression and the differentiation of supernumerary endocrine cells. Our results suggest that Hh proteins influence pancreas specification via inductive interactions from the axial midline rather than through their localized expression in the endodermal cells themselves.
Assuntos
Proteínas de Homeodomínio , Pâncreas/embriologia , Receptores Acoplados a Proteínas G , Transdução de Sinais/fisiologia , Transativadores/metabolismo , Proteínas de Peixe-Zebra , Animais , Expressão Gênica , Proteínas Hedgehog , Receptores de Superfície Celular/genética , Receptores de Superfície Celular/metabolismo , Transdução de Sinais/genética , Receptor Smoothened , Transativadores/genética , Peixe-Zebra/embriologiaRESUMO
BACKGROUND: The Drosophila segment polarity gene hedgehog encodes a member of a family of secreted proteins that are involved in a variety of patterning processes, in both vertebrates and invertebrates. Some of these processes depend upon short-range or contact-dependent interactions, whereas others seem to involve long-range signalling. Two different models have been proposed to account for the execution of these contrasting processes by the same proteins: one postulates that Hedgehog acts exclusively over short distances, its long-range influences being effected through regulation of other signalling factors; the second postulates that different aspects of Hedgehog activity are mediated by distinct forms of the protein that are generated by autoproteolysis. RESULTS: We have investigated these models by mutating the hedgehog coding region such that only the amino-terminal or carboxy-terminal half of the protein is secreted. Deletion of the carboxy-terminal portion has little effect on the signalling activity of the protein, whereas abolishing the secretion of the amino-terminal half leads to a complete loss of signalling. In addition, we find that increases in the level of expression within the normal hedgehog transcriptional domain of either the wild-type protein or the carboxy-terminal-deleted form expand the range of activity to a limited extent, but have only minor effects on cell identity. CONCLUSIONS: In Drosophila, all of the signalling activity of Hedgehog resides in the amino-terminal portion of the protein, the secretion of which is essential for its function. The range of Hedgehog is limited by the close association of the amino-terminal peptide with the cell surface but can be extended by elevating the level of its expression.
Assuntos
Proteínas de Drosophila , Drosophila melanogaster/fisiologia , Fragmentos de Peptídeos/fisiologia , Proteínas/fisiologia , Transdução de Sinais , Sequência de Aminoácidos , Animais , Sequência de Bases , Drosophila melanogaster/embriologia , Drosophila melanogaster/genética , Regulação da Expressão Gênica , Proteínas Hedgehog , Hormônios de Inseto/fisiologia , Larva , Dados de Sequência Molecular , Morfogênese/genética , Proteínas/química , Proteínas Recombinantes/metabolismo , Transcrição Gênica , Asas de Animais/embriologia , Asas de Animais/crescimento & desenvolvimentoRESUMO
Hedgehog (Hh) signalling plays a central role in many developmental processes in both vertebrates and invertebrates [1]. The multipass membrane-spanning proteins Patched (Ptc) [2-4] and Smoothened (Smo) [5-7] have been proposed to act as subunits of a putative Hh receptor complex. According to this view, Smo functions as the transducing subunit, the activity of which is blocked by a direct interaction with the ligand-binding subunit, Ptc [8]. Activation of the intracellular signalling pathway occurs when Hh binds to Ptc [8-11], an event assumed to release Smo from Ptc-mediated inhibition. Evidence for a physical interaction between Smo and Ptc is so far limited to studies of the vertebrate versions of these proteins when overexpressed in tissue culture systems [8,12]. To test this model, we have overexpressed the Drosophila Smo protein in vivo and found that increasing the levels of Smo protein per se was not sufficient for activation of the pathway. Immunohistochemical staining of wild-type and transgenic embryos revealed distinct patterns of Smo distribution, depending on which region of the protein was detected by the antibody. Our findings suggest that Smo is modified to yield a non-functional form and this modification is promoted by Ptc in a non-stoichiometric manner.
Assuntos
Proteínas de Drosophila , Embrião não Mamífero/fisiologia , Proteínas de Insetos/fisiologia , Proteínas de Membrana/fisiologia , Receptores de Superfície Celular/metabolismo , Receptores Acoplados a Proteínas G , Animais , Drosophila/embriologia , Embrião não Mamífero/citologia , Proteínas Hedgehog , Morfogênese , Receptores de Superfície Celular/fisiologia , Proteínas Recombinantes/análise , Transdução de Sinais/fisiologia , Receptor Smoothened , Transfecção , beta-Galactosidase/análiseRESUMO
The tumor suppressor gene patched (ptc) encodes an approximately 140 kDa polytopic transmembrane protein [1-3] [corrected] that binds members of the Hedgehog (Hh) family of signaling proteins [4-6] [corrected] and regulates the activity of Smoothened (Smo), a G protein-coupled receptor-like protein essential for Hh signal transduction [7-9] [corrected]. Ptc contains a sterol-sensing domain (SSD) [10, 11] [corrected], a motif found in proteins implicated in the intracellular trafficking of cholesterol [12] [corrected], and/or other cargoes [13-15] [corrected]. Cholesterol plays a critical role in Hedgehog (Hh) signaling by facilitating the regulated secretion and sequestration of the Hh protein [16] [corrected], to which it is covalently coupled. In addition, cholesterol synthesis inhibitors block the ability of cells to respond to Hh [18, 19] [corrected], and this finding points to an additional requirement for the lipid in regulating downstream components of the Hh signaling pathway. Although the SSD of Ptc has been linked to both the sequestration of, and the cellular response to Hh [16, 20, 21] [corrected], definitive evidence for its function has so far been lacking. Here we describe the identification and characterization of two missense mutations in the SSD of Drosophila Ptc; strikingly, while both mutations abolish Smo repression, neither affects the ability of Ptc to interact with Hh. We speculate that Ptc may control Smo activity by regulating an intracellular trafficking process dependent upon the integrity of the SSD.
Assuntos
Proteínas de Drosophila , Proteínas de Insetos/fisiologia , Proteínas de Membrana/fisiologia , Mutação de Sentido Incorreto , Receptores de Superfície Celular/metabolismo , Receptores Acoplados a Proteínas G , Sequência de Aminoácidos , Animais , Transporte Biológico , Drosophila/genética , Drosophila/crescimento & desenvolvimento , Drosophila/metabolismo , Feminino , Proteínas Hedgehog , Proteínas de Insetos/genética , Proteínas de Insetos/metabolismo , Masculino , Proteínas de Membrana/genética , Proteínas de Membrana/metabolismo , Dados de Sequência Molecular , Transdução de Sinais/fisiologia , Receptor Smoothened , Esteróis , Frações SubcelularesRESUMO
Mutations of the hairy locus in Drosophila may affect both adult chaeta differentiation and embryonic segmentation. In an effort to understand this phenotypic complexity, we have analyzed 30 mutant alleles of the locus. We find that the alleles fall into four groups according to their complementation properties, suggesting a structurally complex locus in which two distinct functions share a common coding region.
RESUMO
How somitic cells become restricted to the muscle fate has been investigated on a number of levels. Classical embryological manipulations have attempted to define the source of inductive signals that control the formation of the myotome. Recently, these studies have converged with others dissecting the role of secreted proteins in embryonic patterning to demonstrate a role for specific peptides in inducing individual cell types of the myotome. Collectively, these investigations have implicated the products of the Wnt, Hedgehog (Hh) and Bone morphogenetic protein (Bmp) gene families as key myogenic regulators; simultaneously controlling both the initiation of myogenesis and the fate of individual myoblasts.
Assuntos
Proteínas de Drosophila , Músculo Esquelético/embriologia , Vertebrados/embriologia , Animais , Diferenciação Celular/genética , Diferenciação Celular/fisiologia , Indução Embrionária/genética , Regulação da Expressão Gênica no Desenvolvimento/genética , Regulação da Expressão Gênica no Desenvolvimento/fisiologia , Proteínas Hedgehog , Proteínas de Insetos/genética , Mesoderma/citologia , Mesoderma/fisiologia , Músculo Esquelético/citologia , Processamento de Proteína Pós-Traducional/genética , Fatores de Transcrição/fisiologiaRESUMO
T-box (tbx) genes constitute a large family of transcriptional regulators involved in developmental patterning processes. In tetrapods, tbx5 has been implicated in specifying forelimb type identity. Here, we report the cloning of the zebrafish tbx5.1 gene and characterise its expression during zebrafish embryogenesis and early larval development of wild type and mutant embryos that affect pectoral fin patterning. tbx5.1 is expressed during development of the heart, the pectoral fins and the eye. Notably, its expression in the lateral plate mesoderm defines a single and continuous region of heart and pectoral fin precursor cells, and constitutes the earliest specific marker for pectoral fin development in the zebrafish.
Assuntos
Regulação da Expressão Gênica no Desenvolvimento , Proteínas com Domínio T/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , DNA Complementar , Humanos , Camundongos , Dados de Sequência Molecular , Homologia de Sequência de Aminoácidos , Distribuição Tecidual , Peixe-ZebraRESUMO
The segment polarity genes patched (ptc) and hedgehog (hh) are thought to encode a receptor and signal molecule respectively, components of a signal transduction pathway that regulates the transcription of the wingless gene in the Drosophila embryo. Here we describe the production of antibodies specific for the products of these two genes and the patterns of protein distribution that they reveal in the developing embryo. The results are consistent with the hh protein being secreted by cells in which it is expressed and support a role for ptc in the reception of the putative hh encoded signal.
Assuntos
Proteínas de Drosophila , Drosophila/metabolismo , Hormônios de Inseto/metabolismo , Proteínas de Membrana/metabolismo , Proteínas/metabolismo , Transdução de Sinais/genética , Animais , Anticorpos Monoclonais , Especificidade de Anticorpos , Drosophila/embriologia , Embrião não Mamífero/metabolismo , Desenvolvimento Embrionário , Receptores de Superfície CelularRESUMO
The Hedgehog signalling pathway is deployed repeatedly during normal animal development and its inappropriate activity is associated with various tumours in human. The serpentine protein Smoothened (Smo) is essential for cells to respond to the Hedeghog (Hh) signal; oncogenic forms of Smo have been isolated from human basal cell carcinomas. Despite similarities with ligand binding G-protein coupled receptors, the molecular basis of Smo activity and its regulation remains unclear. In non-responding cells, Smo is suppressed by the activity of another multipass membrane spanning protein Ptc, which acts as the Hh receptor. In Drosophila, binding of Hh to Ptc has been shown to cause an accumulation of phosphorylated Smo protein and a concomitant stabilisation of the activated form of the Ci transcription factor. Here, we identify domains essential for Smo activity and investigate the sub-cellular distribution of the wild type protein in vivo. We find that deletion of the amino terminus and the juxtamembrane region of the carboxy terminus of the protein result in the loss of normal Smo activity. Using Green Fluorescent Protein (GFP) and horseradish peroxidase fusion proteins we show that Smo accumulates in the plasma membrane of cells in which Ptc activity is abrogated by Hh but is targeted to the degradative pathway in cells where Ptc is active. We further demonstrate that Smo accumulation is likely to be a cause, rather than a consequence, of Hh signal transduction.
Assuntos
Proteínas de Drosophila/biossíntese , Proteínas de Drosophila/química , Proteínas de Drosophila/metabolismo , Regulação da Expressão Gênica no Desenvolvimento , Receptores Acoplados a Proteínas G/biossíntese , Receptores Acoplados a Proteínas G/química , Transdução de Sinais , Alelos , Animais , Proteínas de Bactérias/metabolismo , Células COS , Proteínas Quinases Dependentes de AMP Cíclico/metabolismo , Proteínas de Ligação a DNA/metabolismo , Drosophila melanogaster , Deleção de Genes , Proteínas de Fluorescência Verde/metabolismo , Proteínas Hedgehog , Homozigoto , Peroxidase do Rábano Silvestre/metabolismo , Ligantes , Proteínas Luminescentes/metabolismo , Proteínas de Membrana/metabolismo , Microscopia , Microscopia Confocal , Microscopia Eletrônica de Transmissão , Mutagênese , Proteínas Mutantes Quiméricas , Mutação , Fenótipo , Fosforilação , Mutação Puntual , Ligação Proteica , Conformação Proteica , Estrutura Terciária de Proteína , Receptores de Superfície Celular , Proteínas Recombinantes de Fusão/metabolismo , Receptor Smoothened , Fatores de TranscriçãoRESUMO
Fli-1 is an ETS-domain transcription factor whose locus is disrupted in Ewing's Sarcoma and F-MuLV induced erythroleukaemia. To gain a better understanding of its normal function, we have isolated the zebrafish homologue. Similarities with other vertebrates, in the amino acid sequence and DNA binding properties of Fli-1 from zebrafish, suggest that its function has been conserved during vertebrate evolution. The initial expression of zebrafish fli-1 in the posterior lateral mesoderm overlaps with that of gata2 in a potential haemangioblast population which likely contains precursors of blood and endothelium. Subsequently, fli-1 and gata2 expression patterns diverge, with separate fli-1 and gata2 expression domains arising in the developing vasculature and in sites of blood formation respectively. Elsewhere in the embryo, fli-1 is expressed in sites of vasculogenesis. The expression of fli-1 was investigated in a number of zebrafish mutants, which affect the circulatory system. In cloche, endothelium is absent and blood is drastically reduced. In contrast to the blood and endothelial markers that have been studied previously, fli-1 expression was initiated normally in cloche embryos, indicating that induction of fli-1 is one of the earliest indicators of haemangioblast formation. Furthermore, although fli-1 expression in the trunk was not maintained, the normal expression pattern in the anterior half of the embryo was retained. These anterior cells did not, however, condense to form blood vessels. These data indicate that cloche has previously unsuspected roles at multiple stages in the formation of the vasculature. Analysis of fli-1 expression in midline patterning mutants floating head and squint, confirms a requirement for the notochord in the formation of the dorsal-aorta. The formation of endothelium in one-eyed pinhead, cyclops and squint embryos indicates a novel role for the endoderm in the formation of the axial vein. The phenotype of sonic-you mutants implies a likely role for Sonic Hedgehog in mediating these processes.
Assuntos
Proteínas de Ligação a DNA/genética , Neovascularização Fisiológica/fisiologia , Proteínas Proto-Oncogênicas , Transativadores/genética , Proteínas de Peixe-Zebra , Sequência de Aminoácidos , Animais , Sequência de Bases , Clonagem Molecular , DNA/metabolismo , Endotélio Vascular/citologia , Endotélio Vascular/metabolismo , Fator de Transcrição GATA2 , Expressão Gênica , Células-Tronco Hematopoéticas/metabolismo , Proteínas de Homeodomínio/genética , Humanos , Peptídeos e Proteínas de Sinalização Intracelular , Camundongos , Dados de Sequência Molecular , Mutação , Ligantes da Sinalização Nodal , Proteína Proto-Oncogênica c-fli-1 , Homologia de Sequência de Aminoácidos , Células-Tronco/citologia , Células-Tronco/metabolismo , Fatores de Transcrição/genética , Fator de Crescimento Transformador beta/genética , Peixe-ZebraRESUMO
Animals homozygous for a spontaneously arising allele of the trithorax (trx) gene exhibit highly variegated homoeotic transformations of their thoracic and abdominal segments. This paper retraces the sometimes tortuous history of the analysis of trx function, from the fortuitous recovery of the first trx allele, to the present understanding that trx encodes a highly conserved chromatin binding protein that is required to maintain the expression of the Antennapedia and Bithorax complex genes.