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1.
Proc Natl Acad Sci U S A ; 110(21): 8696-701, 2013 May 21.
Artigo em Inglês | MEDLINE | ID: mdl-23650377

RESUMO

Organisms that use ammonium as the sole nitrogen source discriminate between [(15)N] and [(14)N] ammonium. This selectivity leaves an isotopic signature in their biomass that depends on the external concentration of ammonium. To dissect how differences in discrimination arise molecularly, we examined a wild-type (WT) strain of Escherichia coli K12 and mutant strains with lesions affecting ammonium-assimilatory proteins. We used isotope ratio mass spectrometry (MS) to assess the nitrogen isotopic composition of cell material when the strains were grown in batch culture at either high or low external concentrations of NH3 (achieved by controlling total NH4Cl and pH of the medium). At high NH3 (≥ 0.89 µM), discrimination against the heavy isotope by the WT strain (-19.2‰) can be accounted for by the equilibrium isotope effect for dissociation of NH4(+) to NH3 + H(+). NH3 equilibrates across the cytoplasmic membrane, and glutamine synthetase does not manifest an isotope effect in vivo. At low NH3 (≤ 0.18 µM), discrimination reflects an isotope effect for the NH4(+) channel AmtB (-14.1‰). By making E. coli dependent on the low-affinity ammonium-assimilatory pathway, we determined that biosynthetic glutamate dehydrogenase has an inverse isotope effect in vivo (+8.8‰). Likewise, by making unmediated diffusion of NH3 across the cytoplasmic membrane rate-limiting for cell growth in a mutant strain lacking AmtB, we could deduce an in vivo isotope effect for transport of NH3 across the membrane (-10.9‰). The paper presents the raw data from which our conclusions were drawn and discusses the assumptions underlying them.


Assuntos
Proteínas de Transporte de Cátions/metabolismo , Membrana Celular/metabolismo , Escherichia coli K12/metabolismo , Proteínas de Escherichia coli/metabolismo , Nitrogênio/metabolismo , Compostos de Amônio Quaternário/metabolismo , Transporte de Íons/fisiologia , Isótopos de Nitrogênio/metabolismo
2.
PLoS Biol ; 6(2): e27, 2008 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-18271625

RESUMO

Identifying the genomic regions bound by sequence-specific regulatory factors is central both to deciphering the complex DNA cis-regulatory code that controls transcription in metazoans and to determining the range of genes that shape animal morphogenesis. We used whole-genome tiling arrays to map sequences bound in Drosophila melanogaster embryos by the six maternal and gap transcription factors that initiate anterior-posterior patterning. We find that these sequence-specific DNA binding proteins bind with quantitatively different specificities to highly overlapping sets of several thousand genomic regions in blastoderm embryos. Specific high- and moderate-affinity in vitro recognition sequences for each factor are enriched in bound regions. This enrichment, however, is not sufficient to explain the pattern of binding in vivo and varies in a context-dependent manner, demonstrating that higher-order rules must govern targeting of transcription factors. The more highly bound regions include all of the over 40 well-characterized enhancers known to respond to these factors as well as several hundred putative new cis-regulatory modules clustered near developmental regulators and other genes with patterned expression at this stage of embryogenesis. The new targets include most of the microRNAs (miRNAs) transcribed in the blastoderm, as well as all major zygotically transcribed dorsal-ventral patterning genes, whose expression we show to be quantitatively modulated by anterior-posterior factors. In addition to these highly bound regions, there are several thousand regions that are reproducibly bound at lower levels. However, these poorly bound regions are, collectively, far more distant from genes transcribed in the blastoderm than highly bound regions; are preferentially found in protein-coding sequences; and are less conserved than highly bound regions. Together these observations suggest that many of these poorly bound regions are not involved in early-embryonic transcriptional regulation, and a significant proportion may be nonfunctional. Surprisingly, for five of the six factors, their recognition sites are not unambiguously more constrained evolutionarily than the immediate flanking DNA, even in more highly bound and presumably functional regions, indicating that comparative DNA sequence analysis is limited in its ability to identify functional transcription factor targets.


Assuntos
Blastoderma/metabolismo , Drosophila melanogaster/embriologia , Fatores de Transcrição/metabolismo , Animais , Sítios de Ligação , DNA/metabolismo , Evolução Molecular , MicroRNAs/metabolismo
3.
J Bacteriol ; 192(16): 4089-102, 2010 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-20400551

RESUMO

The Rut pathway is composed of seven proteins, all of which are required by Escherichia coli K-12 to grow on uracil as the sole nitrogen source. The RutA and RutB proteins are central: no spontaneous suppressors arise in strains lacking them. RutA works in conjunction with a flavin reductase (RutF or a substitute) to catalyze a novel reaction. It directly cleaves the uracil ring between N-3 and C-4 to yield ureidoacrylate, as established by both nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry. Although ureidoacrylate appears to arise by hydrolysis, the requirements for the reaction and the incorporation of (18)O at C-4 from molecular oxygen indicate otherwise. Mass spectrometry revealed the presence of a small amount of product with the mass of ureidoacrylate peracid in reaction mixtures, and we infer that this is the direct product of RutA. In vitro RutB cleaves ureidoacrylate hydrolytically to release 2 mol of ammonium, malonic semialdehyde, and carbon dioxide. Presumably the direct products are aminoacrylate and carbamate, both of which hydrolyze spontaneously. Together with bioinformatic predictions and published crystal structures, genetic and physiological studies allow us to predict functions for RutC, -D, and -E. In vivo we postulate that RutB hydrolyzes the peracid of ureidoacrylate to yield the peracid of aminoacrylate. We speculate that RutC reduces aminoacrylate peracid to aminoacrylate and RutD increases the rate of spontaneous hydrolysis of aminoacrylate. The function of RutE appears to be the same as that of YdfG, which reduces malonic semialdehyde to 3-hydroxypropionic acid. RutG appears to be a uracil transporter.


Assuntos
Escherichia coli K12/metabolismo , Proteínas de Escherichia coli/genética , Proteínas de Escherichia coli/metabolismo , Redes e Vias Metabólicas , Nitrogênio/metabolismo , Oxirredutases/genética , Oxirredutases/metabolismo , Uracila/metabolismo , Dióxido de Carbono/metabolismo , Escherichia coli K12/crescimento & desenvolvimento , Proteínas de Escherichia coli/química , Deleção de Genes , Espectroscopia de Ressonância Magnética , Malondialdeído/análogos & derivados , Malondialdeído/metabolismo , Espectrometria de Massas , Modelos Biológicos , Oxirredutases/química , Compostos de Amônio Quaternário/metabolismo
4.
Proc Natl Acad Sci U S A ; 104(47): 18706-11, 2007 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-17998534

RESUMO

The Amt/Mep ammonium channels are trimers in which each monomer contains a long, narrow, hydrophobic pore. Whether the substrate conducted by these pores is NH(3) or NH(4)(+) remains controversial. Substitution of leucine for the highly conserved tryptophan 148 residue at the external opening to Escherichia coli AmtB pores allowed us to address this issue. A strain carrying AmtB(W148L) accumulates much larger amounts of both [(14)C]methylammonium and [(14)C]methylglutamine in a washed cell assay than a strain carrying wild-type AmtB. Accumulation of methylammonium occurs within seconds and appears to reflect channel conductance, whereas accumulation of methylglutamine, which depends on the ATP-dependent activity of glutamine synthetase, increases for many minutes. Concentration of methylammonium was most easily studied in strains that lack glutamine synthetase. It is eliminated by the protonophore carbonyl cyanide m-chlorophenyl hydrazone and is approximately 10-fold higher in the strain carrying AmtB(W148L) than wild-type AmtB. The results indicate that AmtB allows accumulation of CH(3)NH(3)(+) ion in response to the electrical potential across the membrane and that the rate of flux through AmtB(W148L) is approximately 10 times faster than through wild-type AmtB. We infer that both mutant and wild-type proteins also carry NH(4)(+). Contrary to our previous views, we assess that E. coli AmtB does not differ from plant Amt proteins in this regard; both carry ions. We address the role of W148 in decreasing the activity and increasing the selectivity of AmtB and the implications of our findings with respect to the function of Rh proteins, the only known homologues of Amt/Mep proteins.


Assuntos
Proteínas de Transporte de Cátions/metabolismo , Proteínas de Escherichia coli/metabolismo , Escherichia coli/metabolismo , Proteínas de Transporte de Cátions/genética , Cromatografia em Camada Fina , Escherichia coli/citologia , Escherichia coli/genética , Proteínas de Escherichia coli/genética , Regulação Bacteriana da Expressão Gênica , Íons/química , Íons/metabolismo , Lisina/genética , Lisina/metabolismo , Metilaminas/metabolismo , Viabilidade Microbiana , Mutação/genética , Triptofano/genética , Triptofano/metabolismo
5.
Genetics ; 173(4): 2005-19, 2006 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-16702425

RESUMO

The AMT4 locus of the green alga Chlamydomonas reinhardtii, which we mapped to the long arm of chromosome 8, provides a good experimental system for the study of transposition. Most mutations that confer resistance to the toxic ammonium analog methylammonium are in AMT4 and a high proportion of spontaneous mutations are caused by transposon-related events. Among the 15 such events that we have characterized at the molecular level, 9 were associated with insertions of the retrotransposon TOC1, 2 with a small Gulliver-related transposon, and 1 with the Tcr1 transposon. We found that Tcr1 is apparently a foldback transposon with terminal inverted repeats that are much longer and more complex than previously realized. A duplication of Tcr1 yielded a configuration thought to be important for chromosomal evolution. Other mutations in AMT4 were caused by two mobile elements that have not been described before. The sequence of one, which we propose to call the Bill element, indicates that it probably transposes by way of a DNA intermediate and requires functions that it does not encode. The sequence of the other and bioinformatic analysis indicates that it derives from a miniature retrotransposon or TRIM, which we propose to call MRC1 (miniature retrotransposon of Chlamydomonas).


Assuntos
Chlamydomonas reinhardtii/genética , DNA de Algas/genética , Resistência a Medicamentos/genética , Genes de Protozoários/genética , Locos de Características Quantitativas/genética , Retroelementos/genética , Animais , Sequência de Bases , Chlamydomonas reinhardtii/metabolismo , Cromossomos/genética , Resistência a Medicamentos/efeitos dos fármacos , Evolução Molecular , Metilaminas/farmacologia , Dados de Sequência Molecular , Mutação
6.
Genetics ; 170(2): 631-44, 2005 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-15802504

RESUMO

Evidence in several microorganisms indicates that Amt proteins are gas channels for NH(3) and CH(3)NH(2), and this has been confirmed structurally. Chlamydomonas reinhardtii has at least four AMT genes, the most reported for a microorganism. Under nitrogen-limiting conditions all AMT genes are transcribed and Chlamydomonas is sensitive to methylammonium toxicity. All 16 spontaneous methylammonium-resistant mutants that we analyzed had defects in accumulation of [(14)C]methylammonium. Genetic crosses indicated that 12 had lesions in a single locus, whereas two each had lesions in other loci. Lesions in different loci were correlated with different degrees of defect in [(14)C]methylammonium uptake. One mutant in the largest class had an insert in the AMT4 gene, and the insert cosegregated with methylammonium resistance in genetic crosses. The other 11 strains in this class also had amt4 lesions, which we characterized at the molecular level. Properties of the amt4 mutants were clearly different from those of rh1 RNAi lines. They indicated that the physiological substrates for Amt and Rh proteins, the only two members of their protein superfamily, are NH(3) and CO(2), respectively.


Assuntos
Proteínas de Transporte de Cátions/genética , Mutação , Compostos de Amônio Quaternário/metabolismo , Alelos , Sequência de Aminoácidos , Animais , Sequência de Bases , Transporte Biológico , Northern Blotting , Dióxido de Carbono/metabolismo , Proteínas de Transporte de Cátions/fisiologia , Chlamydomonas reinhardtii/genética , Chlamydomonas reinhardtii/metabolismo , Cruzamentos Genéticos , DNA/química , Técnicas Genéticas , Genótipo , Metilaminas/farmacologia , Modelos Genéticos , Dados de Sequência Molecular , Nitrogênio/metabolismo , Peptídeos/química , Fenótipo , Reação em Cadeia da Polimerase , RNA/química , RNA/metabolismo , Interferência de RNA , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Análise de Sequência de DNA , Homologia de Sequência de Aminoácidos , Especificidade por Substrato , Fatores de Tempo , Transcrição Gênica
7.
J Mol Biol ; 339(2): 379-94, 2004 May 28.
Artigo em Inglês | MEDLINE | ID: mdl-15136040

RESUMO

Shigella flexneri temperate bacteriophage Sf6 is of interest in part because its prophage expresses the oac gene that alters the antigenic properties of the surface O-antigen polysaccharide of its host bacterium. We have determined the complete sequence of its 39,044 bp genome. The sequence shows that Sf6 is a member of the canonical lambdoid phage group, and like other phages of this type has a highly mosaic genome. It has chromosomal regions that encode proteins >80% identical with at least 15 different previously characterized lambdoid phages and prophages, but 43% of the genome, including the virion assembly genes, is homologous to the genome of one phage, HK620. An analysis of the nucleotide differences between Sf6 and HK620 indicates that even these similar regions are highly mosaic. This mosaicism suggests ways in which the virion structural proteins might interact with each other. The Sf6 early operons are arranged like a typical lambdoid phage, with "boundary sequences" often found between functional modules in the "metabolic" genome domain. By virtue of high degree of similarity in the encoding genes and their DNA target sites, we predict that the integrase, early transcription anti-terminator, CI and Cro repressors, and CII protein of Sf6 have DNA binding specificities very similar to the homologous proteins encoded by phages HK620, lambda, 434 and P22, respectively. The late operon contains two tRNA genes. The Sf6 terminase genes are unusual. Analysis of in vivo initiation of the DNA packaging series showed that the Sf6 apparatus that recognizes DNA for packaging appears to cleave DNA for initiation of packaging series at many sites within a large region of about 1800 bp that includes a possible pac site. This is unlike previously characterized phage packaging mechanisms.


Assuntos
Bacteriófagos/genética , Cromossomos Bacterianos , Mosaicismo , Shigella flexneri/virologia , Sequência de Bases , DNA Viral , Genoma Viral , Dados de Sequência Molecular , Homologia de Sequência do Ácido Nucleico
8.
PLoS One ; 6(2): e16717, 2011 Feb 25.
Artigo em Inglês | MEDLINE | ID: mdl-21364914

RESUMO

We here develop computational methods to facilitate use of 454 whole genome shotgun sequencing to identify mutations in Escherichia coli K12. We had Roche sequence eight related strains derived as spontaneous mutants in a background without a whole genome sequence. They provided difference tables based on assembling each genome to reference strain E. coli MG1655 (NC_000913). Due to the evolutionary distance to MG1655, these contained a large number of both false negatives and positives. By manual analysis of the dataset, we detected all the known mutations (24 at nine locations) and identified and genetically confirmed new mutations necessary and sufficient for the phenotypes we had selected in four strains. We then had Roche assemble contigs de novo, which we further assembled to full-length pseudomolecules based on synteny with MG1655. This hybrid method facilitated detection of insertion mutations and allowed annotation from MG1655. After removing one genome with less than the optimal 20- to 30-fold sequence coverage, we identified 544 putative polymorphisms that included all of the known and selected mutations apart from insertions. Finally, we detected seven new mutations in a total of only 41 candidates by comparing single genomes to composite data for the remaining six and using a ranking system to penalize homopolymer sequencing and misassembly errors. An additional benefit of the analysis is a table of differences between MG1655 and a physiologically robust E. coli wild-type strain NCM3722. Both projects were greatly facilitated by use of comparative genomics tools in the CoGe software package (http://genomevolution.org/).


Assuntos
Escherichia coli K12/genética , Genética Microbiana/métodos , Genoma Bacteriano , Análise de Sequência de DNA/métodos , Algoritmos , Biologia Computacional/métodos , Mapeamento de Sequências Contíguas/métodos , Escherichia coli/classificação , Escherichia coli/genética , Estudos de Associação Genética , Genômica/métodos , Modelos Biológicos , Anotação de Sequência Molecular/métodos , Polimorfismo Genético , Especificidade da Espécie
9.
Genetics ; 183(4): 1327-40, 2009 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-19596908

RESUMO

The Escherichia coli ammonium channel AmtB is a trimer in which each monomer carries a pore for substrate conduction and a cytoplasmic C-terminal extension of approximately 25 residues. Deletion of the entire extension leaves the protein with intermediate activity, but some smaller lesions in this region completely inactivate AmtB, as do some lesions in its cytoplasmic loops. We here provide genetic evidence that inactivation depends on the essential protease HflB, which appears to cause inactivation not as a protease but as a chaperone. Selection for restored function of AmtB is a positive selection for loss of the ATPase/chaperone activity of HflB and reveals that the conditional lethal phenotype for hflB is cold sensitivity. Deletion of only a few residues from the C terminus of damaged AmtB proteins seems to prevent HflB from acting on them. Either yields the intermediate activity of a complete C-terminal deletion. HflB apparently "tacks" damaged AmtB tails to the adjacent monomers. Knowing that HflB has intervened is prerequisite to determining the functional basis for AmtB inactivation.


Assuntos
Proteases Dependentes de ATP/metabolismo , Proteínas de Transporte de Cátions/genética , Epistasia Genética , Proteínas de Escherichia coli/genética , Proteínas de Escherichia coli/metabolismo , Escherichia coli/genética , Escherichia coli/metabolismo , Chaperonas Moleculares/metabolismo , Proteínas Mutantes/genética , Proteases Dependentes de ATP/química , Sequência de Aminoácidos , Proteínas de Transporte de Cátions/química , Proteínas de Transporte de Cátions/metabolismo , Proteínas de Escherichia coli/química , Modelos Moleculares , Chaperonas Moleculares/química , Dados de Sequência Molecular , Proteínas Mutantes/química , Proteínas Mutantes/metabolismo , Conformação Proteica , Supressão Genética
10.
Genetics ; 183(4): 1341-55, 2009 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-19884311

RESUMO

Ammonium channels, called Amt or Mep, concentrate NH(4)(+) against a gradient. Each monomer of the trimer has a pore through which substrate passes and a C-terminal cytoplasmic extension. The importance of the C-terminal extension to AmtB activity remains unclear. We have described lesions in conserved C-terminal residues that inactivate AmtB and here characterize 38 intragenic suppressors upstream of the C terminus ( approximately 1/3 of total suppressors). Three that occurred repeatedly, including the previously characterized W148L at the pore entry, restored growth at low NH(3) to nearly wild-type levels and hence restored high activity. V116L completely restored function to two of the mutant proteins and, when separated from other lesions, did not damage wild-type AmtB. A179E notably altered folding of AmtB, compensated for all inactivating C-terminal lesions, and damaged wild-type AmtB. V116L and A179E lie at the cytoplasmic end of transmembrane-spanning segments (TM) 3 and 5, respectively, and the proximal part of the C-terminal tail makes intimate contacts with the loops following them before crossing to the adjacent monomer. Collectively, the properties of intragenic suppressor strains lead us to postulate that the C-terminal tail facilitates an oscillation of TM 5 that is required for coordinated pore function and high AmtB activity. Movement of TM 5 appears to control the opening of both the periplasmic entry and the cytoplasmic exit to the pore.


Assuntos
Proteínas de Transporte de Cátions/genética , Proteínas de Transporte de Cátions/metabolismo , Membrana Celular/metabolismo , Proteínas de Escherichia coli/genética , Proteínas de Escherichia coli/metabolismo , Escherichia coli/citologia , Escherichia coli/genética , Proteases Dependentes de ATP/metabolismo , Sequência de Aminoácidos , Proteínas de Transporte de Cátions/química , Eletroforese em Gel de Poliacrilamida , Escherichia coli/metabolismo , Proteínas de Escherichia coli/química , Marcação por Isótopo , Modelos Moleculares , Dados de Sequência Molecular , Nucleotidiltransferases/metabolismo , Proteínas PII Reguladoras de Nitrogênio/metabolismo , Conformação Proteica , Deleção de Sequência , Supressão Genética
11.
Mol Plant ; 1(6): 925-37, 2008 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-19825593

RESUMO

Chlamydomonas reinhardtii strains lacking phytoene synthase, the first enzyme of carotenoid biosynthesis, are white. They lack carotenoid pigments, have very low levels of chlorophyll, and can grow only heterotrophically in the dark. Our electron and fluorescence microscopic studies showed that such a mutant strain (lts1-204) had a proliferated plastid envelope membrane but no stacks of thylakoid membranes within the plastid. It accumulated cytoplasmic compartments that appeared to be autophagous vacuoles filled with membranous material. The lts1 mutants apparently lacked pyrenoid bodies, which normally house ribulose bisphosphate carboxylase-oxygenase (Rubisco), and accumulated many starch granules. Although these mutant strains cannot synthesize the carotenoid and carotenoid-derived pigments present in the phototactic organelle (eyespot), the mutant we examined made a vestigial eyespot that was disorganized and often mislocalized to the posterior end of the cell. The absence of a pyrenoid body, the accumulation of starch, and the disorganization of the eyespot may all result from the absence of thylakoids. The ultrastructure of lts1 mutant strains is similar to but distinct from that of previously described white and yellow mutant strains of C. reinhardtii and is similar to that of naturally colorless algae of the Polytoma group.


Assuntos
Alquil e Aril Transferases/deficiência , Chlamydomonas reinhardtii/enzimologia , Chlamydomonas reinhardtii/ultraestrutura , Pigmentação/fisiologia , Acetatos/farmacologia , Proteínas de Algas/metabolismo , Autofagia/efeitos dos fármacos , Western Blotting , Membrana Celular/ultraestrutura , Núcleo Celular/efeitos dos fármacos , Núcleo Celular/ultraestrutura , Chlamydomonas reinhardtii/efeitos dos fármacos , Chlamydomonas reinhardtii/crescimento & desenvolvimento , Flagelos/efeitos dos fármacos , Flagelos/ultraestrutura , Geranil-Geranildifosfato Geranil-Geraniltransferase , Membranas Intracelulares/efeitos dos fármacos , Membranas Intracelulares/ultraestrutura , Proteínas de Membrana/metabolismo , Microscopia de Fluorescência , Mitocôndrias/efeitos dos fármacos , Mitocôndrias/ultraestrutura , Pigmentação/efeitos dos fármacos , Reprodutibilidade dos Testes , Amido/metabolismo , Tilacoides/efeitos dos fármacos , Tilacoides/metabolismo , Tilacoides/ultraestrutura , Vacúolos/efeitos dos fármacos , Vacúolos/ultraestrutura
12.
Mol Plant ; 1(6): 1007-20, 2008 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-19825599

RESUMO

The major Rhesus (Rh) protein of the green alga Chlamydomonas reinhardtii, Rh1, is homologous to Rh proteins of humans. It is an integral membrane protein involved in transport of carbon dioxide. To localize a fusion of intact Rh1 to the green fluorescent protein (GFP), we used as host a white (lts1) mutant strain of C. reinhardtii, which is blocked at the first step of carotenoid biosynthesis. The lts1 mutant strain accumulated normal amounts of Rh1 heterotrophically in the dark and Rh1-GFP was at the periphery of the cell co-localized with the cytoplasmic membrane dye FM4-64. Although Rh1 carries a potential chloroplast targeting sequence at its N-terminus, Rh1-GFP was clearly not associated with the chloroplast envelope membrane. Moreover, the N-terminal half of the protein was not imported into chloroplasts in vitro and N-terminal regions of Rh1 did not direct import of the small subunit of ribulose bisphosphate carboxylase (SSU). Despite caveats to this interpretation, which we discuss, current evidence indicates that Rh1 is a cytoplasmic membrane protein and that Rh1-GFP is among the first cytoplasmic membrane protein fusions to be obtained in C. reinhardtii. Although lts1 (white) mutant strains cannot be used to localize proteins within sub-compartments of the chloroplast because they lack thylakoid membranes, they should nonetheless be valuable for localizing many GFP fusions in Chlamydomonas.


Assuntos
Proteínas de Algas/química , Chlamydomonas reinhardtii/metabolismo , Citoplasma/metabolismo , Proteínas de Fluorescência Verde/metabolismo , Membranas Intracelulares/metabolismo , Mutação/genética , Proteínas Recombinantes de Fusão/metabolismo , Proteínas de Algas/metabolismo , Sequência de Aminoácidos , Chlamydomonas reinhardtii/citologia , Chlamydomonas reinhardtii/genética , Cloroplastos/metabolismo , DNA Complementar/genética , Fluorescência , Imageamento Tridimensional , Microscopia Confocal , Mitocôndrias/metabolismo , Dados de Sequência Molecular , Sinais Direcionadores de Proteínas , Transporte Proteico , Alinhamento de Sequência , Transformação Genética
13.
Science ; 318(5848): 245-50, 2007 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-17932292

RESUMO

Chlamydomonas reinhardtii is a unicellular green alga whose lineage diverged from land plants over 1 billion years ago. It is a model system for studying chloroplast-based photosynthesis, as well as the structure, assembly, and function of eukaryotic flagella (cilia), which were inherited from the common ancestor of plants and animals, but lost in land plants. We sequenced the approximately 120-megabase nuclear genome of Chlamydomonas and performed comparative phylogenomic analyses, identifying genes encoding uncharacterized proteins that are likely associated with the function and biogenesis of chloroplasts or eukaryotic flagella. Analyses of the Chlamydomonas genome advance our understanding of the ancestral eukaryotic cell, reveal previously unknown genes associated with photosynthetic and flagellar functions, and establish links between ciliopathy and the composition and function of flagella.


Assuntos
Proteínas de Algas/genética , Proteínas de Algas/fisiologia , Evolução Biológica , Chlamydomonas reinhardtii/genética , Genoma , Animais , Chlamydomonas reinhardtii/fisiologia , Cloroplastos/metabolismo , Biologia Computacional , DNA de Algas/genética , Flagelos/metabolismo , Genes , Genômica , Proteínas de Membrana Transportadoras/genética , Proteínas de Membrana Transportadoras/fisiologia , Dados de Sequência Molecular , Família Multigênica , Fotossíntese/genética , Filogenia , Plantas/genética , Proteoma , Análise de Sequência de DNA
14.
Proc Natl Acad Sci U S A ; 103(13): 5114-9, 2006 Mar 28.
Artigo em Inglês | MEDLINE | ID: mdl-16540542

RESUMO

The b1012 operon of Escherichia coli K-12, which is composed of seven unidentified ORFs, is one of the most highly expressed operons under control of nitrogen regulatory protein C. Examination of strains with lesions in this operon on Biolog Phenotype MicroArray (PM3) plates and subsequent growth tests indicated that they failed to use uridine or uracil as the sole nitrogen source and that the parental strain could use them at room temperature but not at 37 degrees C. A strain carrying an ntrB(Con) mutation, which elevates transcription of genes under nitrogen regulatory protein C control, could also grow on thymidine as the sole nitrogen source, whereas strains with lesions in the b1012 operon could not. Growth-yield experiments indicated that both nitrogens of uridine and thymidine were available. Studies with [(14)C]uridine indicated that a three-carbon waste product from the pyrimidine ring was excreted. After trimethylsilylation and gas chromatography, the waste product was identified by mass spectrometry as 3-hydroxypropionic acid. In agreement with this finding, 2-methyl-3-hydroxypropionic acid was released from thymidine. Both the number of available nitrogens and the waste products distinguished the pathway encoded by the b1012 operon from pyrimidine catabolic pathways described previously. We propose that the genes of this operon be named rutA-G for pyrimidine utilization. The product of the divergently transcribed gene, b1013, is a tetracycline repressor family regulator that controls transcription of the b1012 operon negatively.


Assuntos
Escherichia coli/metabolismo , Pirimidinas/metabolismo , Carbono/metabolismo , Proliferação de Células , Biologia Computacional , Escherichia coli/química , Escherichia coli/citologia , Escherichia coli/genética , Regulação Bacteriana da Expressão Gênica , Estrutura Molecular , Análise de Sequência com Séries de Oligonucleotídeos , Óperon/genética , Fenótipo , Pirimidinas/química , Espectrometria de Massas por Ionização por Electrospray
15.
Proc Natl Acad Sci U S A ; 101(20): 7787-92, 2004 May 18.
Artigo em Inglês | MEDLINE | ID: mdl-15096599

RESUMO

Although Rhesus (Rh) proteins are best known as antigens on human red blood cells, they are not restricted to red cells or to mammals, and hence their primary biochemical functions can be studied in more tractable organisms. We previously established that the Rh1 protein of the green alga Chlamydomonas reinhardtii is highly expressed in cultures bubbled with air containing high CO(2) (3%), conditions under which Chlamydomonas grows rapidly. By RNA interference, we have now obtained Chlamydomonas rh mutants (epigenetic), which are among the first in nonhuman cells. These mutants have essentially no mRNA or protein for RH1 and grow slowly at high CO(2), apparently because they fail to equilibrate this gas rapidly. They grow as well as their parental strain in air and on acetate plus air. However, during growth on acetate, rh1 mutants fail to express three proteins that are known to be down-regulated by high CO(2): periplasmic and mitochondrial carbonic anhydrases and a chloroplast envelope protein. This effect is parsimoniously rationalized if the small amounts of Rh1 protein present in acetate-grown cells of the parental strain facilitate leakage of CO(2) generated internally. Together, these results support our hypothesis that the Rh1 protein is a bidirectional channel for the gas CO(2). Our previous studies in a variety of organisms indicate that the only other members of the Rh superfamily, the ammonium/methylammonium transport proteins, are bidirectional channels for the gas NH(3). Physiologically, both types of gas channels can apparently function in acquisition of nutrients and/or waste disposal.


Assuntos
Dióxido de Carbono/metabolismo , Chlamydomonas reinhardtii/crescimento & desenvolvimento , Sistema do Grupo Sanguíneo Rh-Hr/metabolismo , Animais , Chlamydomonas reinhardtii/genética , Chlamydomonas reinhardtii/metabolismo , Expressão Gênica , Fenótipo , Compostos de Amônio Quaternário/metabolismo , Interferência de RNA , RNA Mensageiro/metabolismo , Sistema do Grupo Sanguíneo Rh-Hr/genética
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