RESUMO
Daidzein is a common isoflavone, having multiple biological effects such as anti-inflammation, anti-allergy, and anti-aging. α-Tocopherol is the tocopherol isoform with the highest vitamin E activity including anti-allergic activity and anti-cancer activity. Hesperetin is a flavone, which shows potent anti-inflammatory effects. These compounds have shortcomings, i.e., water-insolubility and poor absorption after oral administration. The glycosylation of bioactive compounds can enhance their water-solubility, physicochemical stability, intestinal absorption, and biological half-life, and improve their bio- and pharmacological properties. They were transformed by cultured Nicotiana tabacum cells to 7-ß-glucoside and 7-ß-gentiobioside of daidzein, and 3'- and 7-ß-glucosides, 3',7-ß-diglucoside, and 7-ß-gentiobioside of hesperetin. Daidzein and α-tocopherol were glycosylated by galactosylation with ß-glucosidase to give 4'- and 7-ß-galactosides of daidzein, which were new compounds, and α-tocopherol 6-ß-galactoside. These nine glycosides showed higher anti-allergic activity, i.e., inhibitory activity toward histamine release from rat peritoneal mast cells, than their respective aglycones. In addition, these glycosides showed higher tyrosinase inhibitory activity than the corresponding aglycones. Glycosylation of daidzein, α-tocopherol, and hesperetin greatly improved their biological activities.
Assuntos
Antialérgicos/síntese química , Cosméticos/síntese química , Glicosídeos/síntese química , Hesperidina/síntese química , Isoflavonas/síntese química , alfa-Tocoferol/síntese química , Animais , Antialérgicos/metabolismo , Biocatálise , Técnicas de Cultura de Células , Cosméticos/metabolismo , Alimento Funcional/análise , Glicosídeos/metabolismo , Glicosilação , Hesperidina/metabolismo , Humanos , Isoflavonas/metabolismo , Masculino , Mastócitos/citologia , Mastócitos/efeitos dos fármacos , Mastócitos/metabolismo , Monofenol Mono-Oxigenase/antagonistas & inibidores , Monofenol Mono-Oxigenase/metabolismo , Células Vegetais/metabolismo , Cultura Primária de Células , Ratos , Ratos Wistar , Solubilidade , Nicotiana/citologia , Nicotiana/metabolismo , alfa-Tocoferol/metabolismoRESUMO
DNA elongation induced by fluidic stress was investigated on a microfluidic chip composed of a large inlet pool and a narrow channel. Through single-DNA observation with fluorescence microscopy, the manner of stretching of individual T4 DNA molecules (166 kbp) was monitored near the area of accelerating flow with narrowing streamlines. The results showed that the DNA long-axis length increased in a sigmoidal manner depending on the magnitude of flow acceleration, or shear, along the DNA chain. To elucidate the physical mechanism of DNA elongation, we performed a theoretical study by adopting a model of a coarse-grained nonlinear elastic polymer chain elongated by shear stress due to acceleration flow along the chain direction.
Assuntos
DNA/química , Técnicas Analíticas Microfluídicas , Modelos MolecularesRESUMO
In the early stage of tooth germ development, the bud of the dental epithelium is invaginated by the underlying mesenchyme, resulting in the formation of a cap-like folded shape. This bud-to-cap transition plays a critical role in determining the steric design of the tooth. The epithelial-mesenchymal interaction within a tooth germ is essential for mediating the bud-to-cap transition. Here, we present a theoretical model to describe the autonomous process of the morphological transition, in which we introduce mechanical interactions among cells. Based on our observations, we assumed that peripheral cells of the dental epithelium bound tightly to each other to form an elastic sheet, and mesenchymal cells that covered the tooth germ would restrict its growth. By considering the time-dependent growth of cells, we were able to numerically show that the epithelium within the tooth germ buckled spontaneously, which is reminiscent of the cap-stage form. The difference in growth rates between the peripheral and interior parts of the dental epithelium, together with the steric size of the tooth germ, were determining factors for the number of invaginations. Our theoretical results provide a new hypothesis to explain the histological features of the tooth germ.
Assuntos
Comunicação Celular , Germe de Dente/citologia , Germe de Dente/fisiologia , Animais , Fenômenos Biomecânicos , Simulação por Computador , Camundongos Endogâmicos C57BL , Modelos Biológicos , Análise Numérica Assistida por ComputadorRESUMO
We performed monomolecular observations on linear and circular giant DNAs (208 kbp) in an aqueous solution by the use of fluorescence microscopy. The results showed that the degree of conformational fluctuation in circular DNA was ca. 40% less than that in linear DNA, although the long-axis length of circular DNA was only 10% smaller than that of linear DNA. Additionally, the relaxation time of a circular chain was shorter than that of a linear chain by at least one order of magnitude. The essential features of this marked difference between linear and circular DNAs were reproduced by numerical simulations on a ribbon-like macromolecule as a coarse-grained model of a long semiflexible, double-helical DNA molecule. In addition, we calculated the radius of gyration of an interacting chain in a circular form on the basis of the mean field model, which provides a better understanding of the present experimental trend than a traditional theoretical equation.
Assuntos
DNA Circular/química , Conformação de Ácido Nucleico , Cromossomos Artificiais Bacterianos/genética , Difusão , Modelos Moleculares , Método de Monte CarloRESUMO
By use of the single-molecule observation, we count the number of DNA double-strand breaks caused by γ-ray irradiation with genome-sized DNA molecules (166 kbp). We find that P1, the number of double-strand breaks (DSBs) per base pair per unit Gy, is nearly inversely proportional to the DNA concentration above a certain threshold DNA concentration. The inverse relationship implies that the total number of DSBs remains essentially constant. We give a theoretical interpretation of our experimental results in terms of attack of reactive species upon DNA molecules, indicating the significance of the characteristics of genome-sized giant DNA as semiflexible polymers for the efficiency of DSBs.
Assuntos
Quebras de DNA de Cadeia Dupla/efeitos da radiação , DNA/genética , Bacteriófago T4/genética , Bacteriófago T4/efeitos da radiação , DNA/química , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Viral/química , DNA Viral/genética , Relação Dose-Resposta à Radiação , Escherichia coli/genética , Escherichia coli/efeitos da radiação , Raios gama , Humanos , Modelos Biológicos , ProbabilidadeRESUMO
It has been established that, upon the addition of multivalent cations, long DNA chains in an aqueous solution exhibit a remarkable discrete transition from a coil state to a compact state at the level of a single chain. In this study, we investigated the polyelectrolyte nature of DNA with the experimental methodology of single-DNA observation, and provide a theoretical interpretation. We examined the effects of co-ions with different valencies (Cl(-), SO4(2-), PO4(3-)) on DNA compaction. As a result, we found that co-ions with a greater valency induce the coil state rather than the compact state. Based on a simple model with mean-field approximation that considered ion pairing, we show how the increase in entropy of small ions contributes to the stability of the compact state, by overcoming entropic penalties such as elastic confinement of the chain and a decrease in the translational freedom of counterions accompanied by charge neutralization.
Assuntos
DNA/química , Conformação de Ácido Nucleico/efeitos dos fármacos , Fosfatos/farmacologia , Cloreto de Sódio/farmacologia , Espermina/farmacologia , Sulfatos/farmacologia , Microscopia de Fluorescência , TermodinâmicaRESUMO
We consider how the DNA coil-globule transition progresses via the formation of a toroidal ring structure. We formulate a theoretical model of this transition as a phenomenon in which an unstable single loop generated as a result of thermal fluctuation is stabilized through association with other loops along a polyelectrolyte chain. An essential property of the chain under consideration is that it follows a wormlike chain model. A toroidal bundle of loop structures is characterized by a radius and a winding number. The statistical properties of such a chain are discussed in terms of the free energy as a function of the fraction of unfolded segments. We also present an actual experimental observation of the coil-globule transition of single giant DNA molecules, T4 DNA (165.5 kbp), with spermidine (3+), where intrachain phase segregation appears at a NaCl concentration of more than 10 mM. Both the theory and experiments lead to two important points. First, the transition from a partially folded state to a completely folded state has the characteristics of a continuous transition, while the transition from an unfolded state to a folded state has the characteristics of a first-order phase transition. Second, the appearance of a partially folded structure requires a folded structure to be less densely packed than in the fully folded compact state.
Assuntos
DNA/química , DNA/metabolismo , Eletrólitos/química , Eletrólitos/metabolismo , Polímeros/química , Polímeros/metabolismo , Conformação de Ácido Nucleico , Desnaturação de Ácido NucleicoRESUMO
DNA is a genetic material found in all life on Earth. DNA is composed of four types of nucleotide subunits, and forms a double-helical one-dimensional polyelectrolyte chain. If we focus on the microscopic molecular structure, DNA is a rigid rod-like molecule. On the other hand, with coarse graining, a long-chain DNA exhibits fluctuating behavior over the whole molecule due to thermal fluctuation. Owe to its semiflexible nature, individual giant DNA molecule undergoes a large discrete transition in the higher-order structure. In this folding transition into a compact state, small ions in the solution have a critical effect, since DNA is highly charged. In the present article, we interpret the characteristic features of DNA compaction while paying special attention to the role of small ions, in relation to a variety of single-chain morphologies generated as a result of compaction.
Assuntos
DNA/química , Conformação de Ácido Nucleico , Bacteriófago T4/metabolismo , Cátions/química , Simulação por Computador , DNA/ultraestrutura , DNA Viral , Eletrólitos/química , Microscopia de Força Atômica , Modelos Químicos , Água/químicaRESUMO
The photoinduced motion of an oil droplet on an aqueous solution under local irradiation by a green laser is reported. The results showed that a repulsive force is generated on pure water, while an attractive force is observed with an aqueous solution containing a surfactant. The driving force is discussed in terms of a thermal Marangoni effect. The switching on the photothermal effect is interpreted by taking into account the advection caused by the spatial gradient of the surface tension under local heating by a laser. A numerical model revealed that the geometrical profile of the surface tension around the droplet determines the mode of advection around the droplet and causes switching in the direction of migrations.
RESUMO
We investigated the folding transition between an elongated coil state and a compact state on a single polymer chain confined in a small space with different stiffness with the aid of Monte Carlo simulation. In a flexible polymer, the folding transition is retarded in a confined space. In contrast, the transition is promoted for a semiflexible chain, in which the discontinuity of the volume change occupied by a single chain is diminished by confinement. This unique confinement effect is interpreted in terms of conformational entropy and self-avoiding repulsive interaction.
Assuntos
Modelos Moleculares , Conformação Molecular , Polímeros/química , Método de Monte CarloRESUMO
Light irradiation can induce the vectorial motion of an aerosol particle. This phenomenon is often explained in terms of inelastic collision between gas molecules and the aerosol particle under a temperature gradient. We considered the photophoresis of a micron-sized liquid droplet in a rarefied gas atmosphere based on the Boltzmann equation for the atmosphere coupled with the Navier-Stokes equation for the droplet. Two features attributable to induced internal flow in the droplet are analyzed: the contribution of homogeneous energy inflow to the motion of the droplet and the nonlinear scaling of the photophoretic velocity depending on the irradiated light intensity.
RESUMO
Recently, it has been revealed that a semiflexible polyelectrolyte chain can form a partially folded conformation stably as a result of an electrostatic interaction. Interestingly, there are cases where the appearance of this structure requires a high-salt condition of a solution. In order to solve this problem, we consider the double equilibrium of the formation of loops and their aggregation on a single-chain polymer. First, an aggregate with a typical surface energy is examined as a test case. The basic nature of the folding transition is discussed with regard to the chemical potential of loop structures. Next, we consider a charged aggregate for which the interior is completely neutralized by counter ions. In this model, a partially folded chain appears with a high-salt condition. Based on this model, screened interactions between surface charges and a toroidal shape of a folded structure are considered essential factors bihind this phenomenon.
Assuntos
Biopolímeros/química , Modelos Químicos , Modelos Moleculares , Solventes/química , Simulação por Computador , Conformação MolecularRESUMO
The structure of two-dimensional (2D) hard-sphere fluids on a cylindrical surface is investigated by means of the Ornstein-Zernike integral equation with the Percus-Yevick and the hypernetted-chain approximation. The 2D cylindrical coordinate breaks the spherical symmetry. Hence, the pair-correlation function is reformulated as a two-variable function to account for the packing along and around the cylinder. Detailed pair-correlation function calculations based on the two integral equation theories are compared with Monte Carlo simulations. In general, the Percus-Yevick theory is more accurate than the hypernetted-chain theory, but exceptions are observed for smaller cylinders. Moreover, analysis of the angular-dependent contact values shows that particles are preferentially packed anisotropically. The origin of such an anisotropic packing is driven by the entropic effect because the energy of all the possible system configurations of a dense hard-sphere fluid is the same. In addition, the anisotropic packing observed in our model studies serves as a basis for linking the close packing with the morphology of an ordered structure for particles adsorbed onto a cylindrical nanotube.